MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3201–3300 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3179 992.5799 1983.1452
3180 992.8117 1983.6088
3211 669.7326 2006.1760
3215 670.0647 2007.1723
3217 670.3983 2008.1731
3236 674.0550 2019.1432
3237 674.0580 2019.1522
3250 675.4089 2023.2049
3305 686.3891 2056.1455
3325 692.7192 2075.1358
3326 692.7201 2075.1385
3328 693.3871 2077.1395
3329 520.3152 2077.2317
3330 520.3156 2077.2333
3337 695.0551 2082.1435
3340 696.0182 2085.0328
3354 697.7406 2090.2000
3362 524.8184 2095.2445
3376 1053.7822 2105.5498
3424 710.4197 2128.2373
3434 712.4049 2134.1929
3441 713.3871 2137.1395
3463 1074.6552 2147.2958
3468 717.3887 2149.1443
3475 718.4150 2152.2232
3490 1083.5507 2165.0868
3493 723.7316 2168.1730
3494 1085.5952 2169.1758
3495 724.0663 2169.1771
3512 544.5361 2174.1153
3518 727.0806 2178.2200
3576 737.0707 2208.1903
3577 737.0918 2208.2536
3579 737.3777 2209.1113
3615 746.7286 2237.1640
3616 746.7298 2237.1676
3621 1122.1243 2242.2340
3622 748.4190 2242.2352
3628 562.0588 2244.2061
3633 750.0952 2247.2638
3636 750.4468 2248.3186
3643 751.4294 2251.2664
3651 754.3953 2260.1641
3654 755.4178 2263.2316
3678 762.7822 2285.3248
3681 764.3948 2290.1626
3690 765.4316 2293.2730
3695 766.4325 2296.2757
3704 768.0392 2301.0958
3719 1154.0553 2306.0960
3736 463.6562 2313.2446
3737 579.3215 2313.2569
3739 463.8574 2314.2506
3752 777.4475 2329.3207
3753 779.0131 2334.0175
3761 781.4146 2341.2220
3764 1173.6907 2345.3668
3765 1173.6945 2345.3744
3766 782.8002 2345.3788
3767 782.8010 2345.3812
3770 784.4645 2350.3717
3772 785.4490 2353.3252
3775 786.4386 2356.2940
3788 789.1013 2364.2821
3816 1195.2103 2388.4060
3836 803.0776 2406.2110
3838 805.1303 2412.3691
3839 805.1304 2412.3694
3841 604.3361 2413.3153
3842 805.4595 2413.3567
3844 805.8022 2414.3848
3849 806.7517 2417.2333
3856 605.3365 2417.3169
3861 808.4608 2422.3606
3869 813.1096 2436.3070
3874 610.8054 2439.1925
3877 611.2974 2441.1605
3880 816.1512 2445.4318
3881 816.4172 2446.2298
3895 823.1387 2466.3943
3904 827.4644 2479.3714
3912 830.4687 2488.3843
3913 623.1071 2488.3993
3914 830.4777 2488.4113
3915 831.4254 2491.2544
3928 836.4247 2506.2523
3934 837.4521 2509.3345
3935 837.4653 2509.3741
3937 837.4661 2509.3765
3938 837.4681 2509.3825
3940 837.7913 2510.3521
3941 837.8011 2510.3815
3943 839.1446 2514.4120
3950 842.1395 2523.3967
3952 842.4592 2524.3558
3955 844.4307 2530.2703
3965 849.8091 2546.4055
3974 639.6101 2554.4113
3975 640.3418 2557.3381
3980 641.8585 2563.4049
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