MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
241 519.6255 518.6182
242 522.2712 521.2639
243 524.2372 523.2299
244 527.6387 526.6314
245 529.2871 528.2798
252 533.1218 532.1145
253 537.8004 536.7931
254 538.3184 537.3111
255 544.2919 543.2846
261 552.8251 551.8178
262 555.1040 554.0967
263 555.1058 554.0985
264 557.0794 556.0721
265 557.0818 556.0745
274 567.3196 566.3123
275 568.0024 566.9951
276 569.1197 568.1124
278 569.7974 568.7901
279 571.0682 570.0609
280 571.0703 570.0630
281 571.8078 570.8005
287 581.8282 580.8209
295 586.8548 585.8475
299 599.6764 598.6691
300 600.0120 599.0047
343 618.8094 617.8021
344 621.8359 620.8286
467 683.8615 682.8542
558 723.3613 722.3540
594 737.8589 736.8516
607 745.9124 744.9051
619 751.8557 750.8484
620 752.3832 751.3759
626 759.0790 758.0717
654 765.3919 764.3846
655 765.4330 764.4257
682 776.4208 775.4135
703 785.9294 784.9221
720 793.9300 792.9227
743 801.9537 800.9464
749 807.9477 806.9404
757 813.8995 812.8922
764 817.9401 816.9328
766 818.4419 817.4346
804 836.9447 835.9374
805 837.1247 836.1174
862 856.9532 855.9459
883 861.9592 860.9519
899 872.4425 871.4352
1009 918.4720 917.4647
1249 1005.0532 1004.0459
1254 1006.7820 1005.7747
1416 1083.2784 1082.2711
1437 546.8095 1091.6044
1473 557.3683 1112.7220
1536 569.3579 1136.7012
1555 382.9265 1145.7577
1750 410.9347 1229.7823
1995 676.9224 1351.8302
2081 463.9589 1388.8549
2094 349.9470 1395.7589
2159 713.1525 1424.2904
2184 720.3999 1438.7852
2205 484.3293 1449.9661
2222 730.9135 1459.8124
2227 489.6347 1465.8823
2315 755.3884 1508.7622
2368 766.6823 1531.3500
2372 383.9888 1531.9261
2373 383.9889 1531.9265
2438 392.4600 1565.8109
2452 787.4836 1572.9526
2473 790.9689 1579.9232
2517 802.9601 1603.9056
2534 806.0114 1610.0082
2571 809.9976 1617.9806
2597 544.6599 1630.9579
2610 546.9962 1637.9668
2620 821.4634 1640.9122
2662 834.1959 1666.3772
2703 842.4060 1682.7974
2707 562.6559 1684.9459
2714 844.4849 1686.9552
2748 852.4757 1702.9368
2784 861.2086 1720.4026
2815 867.5023 1732.9900
2834 879.9077 1757.8008
2849 588.6682 1762.9828
2868 887.4522 1772.8898
2907 600.3361 1797.9865
2951 455.7205 1818.8529
2952 456.4897 1821.9297
3002 619.6191 1855.8355
3082 636.3448 1906.0126
3086 637.3643 1909.0711
3095 479.7757 1915.0737
3112 966.5468 1931.0790
3118 645.6891 1934.0455
3127 973.5063 1944.9980
3161 660.3871 1978.1395
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