MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
108 398.2471 397.2398
109 399.2314 398.2241
110 399.2373 398.2300
111 401.2873 400.2800
112 401.2881 400.2808
113 401.2885 400.2812
114 401.2888 400.2815
115 401.2893 400.2820
116 401.7265 400.7192
117 402.2179 401.2106
118 402.2477 401.2404
119 402.2478 401.2405
120 403.2313 402.2240
122 403.2319 402.2246
124 404.2517 403.2444
125 407.2280 406.2207
126 407.2281 406.2208
127 407.2282 406.2209
128 407.2284 406.2211
129 407.2285 406.2212
130 407.2287 406.2214
131 407.7444 406.7371
132 409.2437 408.2364
133 409.2439 408.2366
134 409.2440 408.2367
135 409.2441 408.2368
136 409.2442 408.2369
137 409.2443 408.2370
138 409.2446 408.2373
139 416.2632 415.2559
140 416.2632 415.2559
141 416.2634 415.2561
142 416.2639 415.2566
143 416.2881 415.2808
144 416.2883 415.2810
145 416.2888 415.2815
146 416.2889 415.2816
151 418.2675 417.2602
152 421.2572 420.2499
153 421.2580 420.2507
154 423.2946 422.2873
155 425.2282 424.2209
156 425.2387 424.2314
157 425.2390 424.2317
158 425.2396 424.2323
159 425.2398 424.2325
160 430.2791 429.2718
161 430.2793 429.2720
162 430.2796 429.2723
163 430.2797 429.2724
164 430.3035 429.2962
165 430.3038 429.2965
166 430.3041 429.2968
167 430.3046 429.2973
168 430.3047 429.2974
169 430.8579 429.8506
170 432.2590 431.2517
171 432.2593 431.2520
172 433.7040 432.6967
173 437.2168 436.2095
174 440.7321 439.7248
175 443.2505 442.2432
176 444.2947 443.2874
177 444.2947 443.2874
178 444.2949 443.2876
179 446.2737 445.2664
180 446.2743 445.2670
181 446.2745 445.2672
182 446.2996 445.2923
183 447.7397 446.7324
184 448.2566 447.2493
185 450.2484 449.2411
186 451.2679 450.2606
187 451.2685 450.2612
188 451.2688 450.2615
189 457.7762 456.7689
190 458.3124 457.3051
191 460.2934 459.2861
192 460.3145 459.3072
193 462.2361 461.2288
194 472.3261 471.3188
202 475.2568 474.2495
203 475.9592 474.9519
204 476.2122 475.2049
205 478.7816 477.7743
206 482.2705 481.2632
207 484.3293 483.3220
208 485.7841 484.7768
215 489.9468 488.9395
216 493.2792 492.2719
217 496.2855 495.2782
226 506.3108 505.3035
227 506.3108 505.3035
228 508.2906 507.2833
229 510.2369 509.2296
230 510.2382 509.2309
231 510.2390 509.2317
232 510.2812 509.2739
233 511.2894 510.2821
234 515.3336 514.3263
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