MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 201–300 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1693 606.3456 1210.6766 1210.6670 7.98 0 25 0.81 +1Score > 38 indicates identity
Score > 36 indicates homology		 QVTINDLPVGR
1790 625.3260 1248.6374 1248.6350 1.95 1 25 0.34 +1Score > 42 indicates identity
Score > 32 indicates homology		 ATFLQVRVQGE + 2 Deamidated (NQ)
1051 310.5132 928.5178 928.5342 -17.7 1 25 0.94 +1Score > 41 indicates identity
Score > 37 indicates homology		 KVVTPQTR + Deamidated (NQ)
622 377.2176 752.4206 752.4181 3.42 0 24 0.12 +1Score > 38 indicates identity
Score > 28 indicates homology		 ALDVHAK
350 313.1905 624.3664 624.3635 4.68 0 24 0.071 +1Score > 37 indicates identity
Score > 25 indicates homology		 FFIAK
1495 374.5378 1120.5916 1120.5764 13.5 0 24 0.97 +1Score > 41 indicates identity
Score > 37 indicates homology		 KPSYNVGINK + 2 Deamidated (NQ)
495 350.7223 699.4300 699.4167 19.1 1 24 0.74 +1Score > 41 indicates identity
Score > 36 indicates homology		 AIIEVGV
671 388.2028 774.3910 774.4058 -19.1 1 24 0.24 +1Score > 44 indicates identity
Score > 31 indicates homology		 VECVLR
282 572.3541 571.3468 571.3442 4.60 0 24 0.18 +1Score > 42 indicates identity
Score > 29 indicates homology		 AGAVVR
987 456.7320 911.4494 911.4461 3.68 1 24 0.11 +1Score > 40 indicates identity
Score > 27 indicates homology		 ESVIHDGR
581 365.2303 728.4460 728.4432 3.88 1 24 0.6 +1Score > 42 indicates identity
Score > 35 indicates homology		 VAIEAVK
2474 791.3884 1580.7622 1580.7769 -9.30 1 24 0.32 +1Score > 41 indicates identity
Score > 32 indicates homology		 QDIQKLFSTWCR
1321 345.5029 1033.4869 1033.4862 0.61 1 24 0.16 +1Score > 41 indicates identity
Score > 29 indicates homology		 EMDAGVSGLR
1014 460.2543 918.4940 918.4844 10.5 1 24 0.69 +1Score > 41 indicates identity
Score > 35 indicates homology		 IQMELIR + Deamidated (NQ); Oxidation (M)
789 415.7429 829.4712 829.4657 6.64 0 24 0.83 +1Score > 42 indicates identity
Score > 36 indicates homology		 QINNITK
2132 707.3747 1412.7348 1412.7147 14.3 0 24 0.7 +1Score > 40 indicates identity
Score > 35 indicates homology		 NAVVLLNTQGEPR + 3 Deamidated (NQ)
442 673.4001 672.3928 672.3918 1.45 1 24 0.84 +1Score > 43 indicates identity
Score > 36 indicates homology		 AGQLKR + Deamidated (NQ)
785 414.7475 827.4804 827.4753 6.26 0 24 0.48 +1Score > 40 indicates identity
Score > 33 indicates homology		 LPSADVVK
431 336.2270 670.4394 670.4377 2.54 0 24 0.19 +1Score > 37 indicates identity
Score > 29 indicates homology		 VAGAILK
2011 679.8689 1357.7232 1357.6990 17.9 1 24 0.3 +1Score > 40 indicates identity
Score > 31 indicates homology		 NVITWQELQAR + Deamidated (NQ)
1873 432.2772 1293.8098 1293.8132 -2.69 1 24 0.11 +1Score > 27 indicates identity QAVRALVLVPTK
711 395.7015 789.3884 789.3981 -12.2 0 24 0.88 +1Score > 43 indicates identity
Score > 36 indicates homology		 SDNVLSR
2397 387.9576 1547.8013 1547.8308 -19.0 1 24 0.69 +1Score > 40 indicates identity
Score > 35 indicates homology		 IFVDTDSDIRLVR
1438 547.2839 1092.5532 1092.5564 -2.85 1 24 0.2 +1Score > 42 indicates identity
Score > 30 indicates homology		 LAGEFTQATR
1137 479.8030 957.5914 957.5859 5.83 0 24 0.22 +1Score > 34 indicates identity
Score > 30 indicates homology		 IILSGNTIK
1238 500.2848 998.5550 998.5509 4.20 1 24 0.75 +1Score > 39 indicates identity
Score > 35 indicates homology		 QLQEIAAAR
925 442.2374 882.4602 882.4559 4.89 0 24 0.87 +1Score > 39 indicates identity
Score > 36 indicates homology		 SNVDVPPR
501 351.6933 701.3720 701.3821 -14.3 0 24 0.67 +1Score > 45 indicates identity
Score > 35 indicates homology		 GGGGSLVR
1487 560.8058 1119.5970 1119.5924 4.15 0 24 0.38 +1Score > 41 indicates identity
Score > 32 indicates homology		 GAVVAYANDLK
762 408.2356 814.4566 814.4548 2.21 1 24 0.7 +1Score > 43 indicates identity
Score > 35 indicates homology		 EIIIDGR
1193 491.7404 981.4662 981.4477 18.9 0 24 0.31 +1Score > 41 indicates identity
Score > 31 indicates homology		 QFVQAMIE + Deamidated (NQ); Oxidation (M)
630 380.2354 758.4562 758.4538 3.23 1 24 0.91 +1Score > 40 indicates identity
Score > 36 indicates homology		 IVETAVK
629 380.2353 758.4560 758.4538 2.97 0 24 0.65 +1Score > 40 indicates identity
Score > 34 indicates homology		 VTLLGQK + Deamidated (NQ)
1595 390.2024 1167.5854 1167.5884 -2.58 1 24 0.37 +1Score > 40 indicates identity
Score > 32 indicates homology		 QGSHQQLLEK + Deamidated (NQ)
1169 486.7563 971.4980 971.5036 -5.70 1 24 0.39 +1Score > 41 indicates identity
Score > 32 indicates homology		 QLIQQQRG + 2 Deamidated (NQ)
368 632.3753 631.3680 631.3653 4.30 1 23 0.17 +1Score > 41 indicates identity
Score > 28 indicates homology		 KQVTR + Deamidated (NQ)
1061 466.2642 930.5138 930.5134 0.47 1 23 0.38 +1Score > 42 indicates identity
Score > 32 indicates homology		 KLTQAQNK + Deamidated (NQ)
451 338.1882 674.3618 674.3599 2.90 1 23 0.15 +1Score > 44 indicates identity
Score > 28 indicates homology		 ATVGEAK
551 360.1780 718.3414 718.3432 -2.44 0 23 0.94 +1Score > 43 indicates identity
Score > 36 indicates homology		 APTMQR + Oxidation (M)
388 324.1671 646.3196 646.3187 1.47 0 23 0.68 +1Score > 44 indicates identity
Score > 34 indicates homology		 SWQAR
1234 499.3060 996.5974 996.5855 12.0 1 23 0.49 +1Score > 35 indicates identity
Score > 33 indicates homology		 EVIAIDLPK
651 382.7332 763.4518 763.4480 5.10 0 23 0.45 +1Score > 35 indicates identity
Score > 32 indicates homology		 IINYIK + Deamidated (NQ)
1800 629.8571 1257.6996 1257.7002 -0.46 0 23 0.7 +1Score > 39 indicates identity
Score > 34 indicates homology		 LVQIIMGQNIK + 2 Deamidated (NQ)
3482 721.3838 2161.1296 2161.0903 18.2 0 23 0.06 +1Score > 38 indicates identity
Score > 24 indicates homology		 TDPYVTPGSVINNLSINTVR + 2 Deamidated (NQ)
1301 342.8575 1025.5507 1025.5368 13.5 1 23 0.28 +1Score > 39 indicates identity
Score > 30 indicates homology		 AFKFPTMGK
542 358.2285 714.4424 714.4500 -10.6 1 23 0.94 +1Score > 41 indicates identity
Score > 35 indicates homology		 LTRGLR
1730 408.5610 1222.6612 1222.6710 -8.02 0 23 0.19 +1Score > 39 indicates identity
Score > 28 indicates homology		 LNLFLYINGR + Deamidated (NQ)
474 344.7041 687.3936 687.3915 3.09 1 23 0.65 +1Score > 45 indicates identity
Score > 34 indicates homology		 LGLTER
624 379.2324 756.4502 756.4646 -19.0 0 23 0.18 +1Score > 42 indicates identity
Score > 28 indicates homology		 KPLFPR
437 336.7018 671.3890 671.3827 9.45 1 23 0.46 +1Score > 42 indicates identity
Score > 32 indicates homology		 AGVGRGR
1205 494.7848 987.5550 987.5726 -17.8 1 23 0.75 +1Score > 40 indicates identity
Score > 34 indicates homology		 LPHPAARAR
798 417.2505 832.4864 832.4807 6.93 1 23 0.24 +1Score > 37 indicates identity
Score > 29 indicates homology		 IFGEVIR
867 429.7397 857.4648 857.4606 4.90 1 23 0.65 +1Score > 42 indicates identity
Score > 34 indicates homology		 INENVIR + Deamidated (NQ)
2430 781.4619 1560.9092 1560.9167 -4.77 0 23 0.21 +1Score > 32 indicates identity
Score > 29 indicates homology		 IFLFLAILLQQLE + Deamidated (NQ)
3505 725.0691 2172.1855 2172.1711 6.60 1 23 0.14 +1Score > 35 indicates identity
Score > 27 indicates homology		 VDTALDVLLELIVLNASAMR + Deamidated (NQ); Oxidation (M)
2519 803.9279 1605.8412 1605.8436 -1.47 1 23 0.31 +1Score > 39 indicates identity
Score > 30 indicates homology		 YQLVIEGMVLAGQGK + Deamidated (NQ)
1245 502.2583 1002.5020 1002.4982 3.87 0 23 0.54 +1Score > 42 indicates identity
Score > 33 indicates homology		 IANISGDGQK + Deamidated (NQ)
2976 920.4615 1838.9084 1838.9131 -2.55 1 23 0.3 +1Score > 40 indicates identity
Score > 30 indicates homology		 AFLMRCDLLATGTTGGR
1310 343.5318 1027.5736 1027.5886 -14.7 1 23 0.99 +1Score > 40 indicates identity
Score > 35 indicates homology		 ARQTAAIAAR
2593 816.4235 1630.8324 1630.8236 5.41 1 23 0.057 +1Score > 40 indicates identity
Score > 23 indicates homology		 DVVMDGIQSPIKADK + Oxidation (M)
1414 540.3040 1078.5934 1078.6022 -8.15 1 23 0.74 +1Score > 39 indicates identity
Score > 34 indicates homology		 IISQQFKSK + Deamidated (NQ)
631 380.2354 758.4562 758.4650 -11.6 0 23 0.33 +1Score > 40 indicates identity
Score > 30 indicates homology		 VTLLASR
1334 346.8503 1037.5291 1037.5115 16.9 0 23 0.27 +1Score > 40 indicates identity
Score > 30 indicates homology		 GGPGGGRPGGGGR
1647 594.3212 1186.6278 1186.6346 -5.68 1 23 0.79 +1Score > 41 indicates identity
Score > 34 indicates homology		 AKASLIAQAWE
1222 497.8391 993.6636 993.6586 5.03 0 23 0.078 +1Score > 24 indicates identity KPLVVLTPK
1315 1031.5145 1030.5072 1030.5005 6.52 0 23 0.89 +1Score > 41 indicates identity
Score > 35 indicates homology		 LTDPMPVDK + Oxidation (M)
371 316.7036 631.3926 631.3905 3.45 0 23 0.41 +1Score > 31 indicates identity ITGTIK
1066 468.2758 934.5370 934.5348 2.37 1 23 0.11 +1Score > 37 indicates identity
Score > 26 indicates homology		 GRHPLLDK
2586 814.9652 1627.9158 1627.9144 0.87 1 23 0.06 +1Score > 35 indicates identity
Score > 23 indicates homology		 LGINASIGIANSKVAAK + 2 Deamidated (NQ)
650 382.7210 763.4274 763.4228 6.08 1 23 0.12 +1Score > 39 indicates identity
Score > 26 indicates homology		 IIAEYR
397 652.3084 651.3011 651.3010 0.20 0 23 0.14 +1Score > 40 indicates identity
Score > 27 indicates homology		 MATASR + Oxidation (M)
2629 550.6290 1648.8652 1648.8533 7.23 1 23 0.089 +1Score > 39 indicates identity
Score > 25 indicates homology		 LAELAQLQDQLDHR
435 672.3811 671.3738 671.3715 3.52 0 23 0.9 +1Score > 40 indicates identity
Score > 35 indicates homology		 QQGAIR
1610 586.8522 1171.6898 1171.6812 7.38 1 23 0.6 +1Score > 36 indicates identity
Score > 33 indicates homology		 VNNIKITQIK + 2 Deamidated (NQ)
637 380.7206 759.4266 759.4126 18.4 0 22 0.59 +1Score > 42 indicates identity
Score > 33 indicates homology		 LSQGNLK + Deamidated (NQ)
3309 687.3580 2059.0522 2059.0143 18.4 1 22 0.091 +1Score > 39 indicates identity
Score > 25 indicates homology		 TTDLQTTLQLSMKAIQHE + 2 Deamidated (NQ)
2520 803.9289 1605.8432 1605.8436 -0.23 1 22 0.15 +1Score > 39 indicates identity
Score > 27 indicates homology		 YQLVIEGMVLAGQGK + Deamidated (NQ)
236 516.2931 515.2858 515.2816 8.26 0 22 0.51 +1Score > 41 indicates identity
Score > 32 indicates homology		 AGAAAR
1001 458.2829 914.5512 914.5437 8.29 0 22 0.43 +1Score > 38 indicates identity
Score > 31 indicates homology		 LGGDLSLIK
1324 518.2779 1034.5412 1034.5284 12.4 1 22 0.66 +1Score > 41 indicates identity
Score > 33 indicates homology		 LFEQIAANK + 2 Deamidated (NQ)
742 401.2518 800.4890 800.4868 2.79 1 22 0.85 +1Score > 40 indicates identity
Score > 34 indicates homology		 KLAVSQR
353 314.6758 627.3370 627.3340 4.83 0 22 0.23 +1Score > 38 indicates identity
Score > 28 indicates homology		 IGADPR
1916 439.8839 1316.6299 1316.6096 15.4 0 22 0.78 +1Score > 41 indicates identity
Score > 34 indicates homology		 NATSDILVSPGNE + Deamidated (NQ)
1804 631.3569 1260.6992 1260.6826 13.2 1 22 0.79 +1Score > 39 indicates identity
Score > 34 indicates homology		 FQLITRNLQE
1879 432.9159 1295.7259 1295.7197 4.74 1 22 0.29 +1Score > 37 indicates identity
Score > 30 indicates homology		 QGKAGGAEPLLGAK
548 359.1928 716.3710 716.3817 -14.8 0 22 0.74 +1Score > 44 indicates identity
Score > 34 indicates homology		 LAGQTAR + Deamidated (NQ)
636 380.7200 759.4254 759.4126 16.9 0 22 0.63 +1Score > 42 indicates identity
Score > 33 indicates homology		 INSADLK
1302 514.2581 1026.5016 1026.4870 14.3 0 22 0.15 +1Score > 40 indicates identity
Score > 27 indicates homology		 TTGAVSYTVE
2157 712.8661 1423.7176 1423.7347 -12.0 0 22 0.24 +1Score > 41 indicates identity
Score > 29 indicates homology		 LGGFFGTQQGLIGK + 2 Deamidated (NQ)
3929 837.0778 2508.2116 2508.1737 15.1 1 22 0.69 +1Score > 38 indicates identity
Score > 33 indicates homology		 RMGQSGCDIVSVDWTVDLLDAR + Oxidation (M)
1655 398.2012 1191.5818 1191.5884 -5.57 1 22 0.62 +1Score > 41 indicates identity
Score > 33 indicates homology		 GAEGGLDFGTLR
1661 399.8719 1196.5939 1196.6037 -8.21 0 22 0.78 +1Score > 40 indicates identity
Score > 34 indicates homology		 AINDAVIHTDK + Deamidated (NQ)
1032 463.2813 924.5480 924.5392 9.52 0 22 0.085 +1Score > 36 indicates identity
Score > 24 indicates homology		 GLQIAAVPR + Deamidated (NQ)
718 397.2108 792.4070 792.4017 6.69 1 22 0.34 +1Score > 42 indicates identity
Score > 30 indicates homology		 FEQAGLK + Deamidated (NQ)
1858 644.3289 1286.6432 1286.6652 -17.1 0 22 0.078 +1Score > 41 indicates identity
Score > 24 indicates homology		 QALMQLGQQLR + 2 Deamidated (NQ)
381 322.2115 642.4084 642.4064 3.14 0 22 0.84 +1Score > 41 indicates identity
Score > 34 indicates homology		 AIAGLAK
1372 529.8052 1057.5958 1057.5768 18.1 1 22 0.57 +1Score > 41 indicates identity
Score > 32 indicates homology		 QTQAILGAEK
944 894.4712 893.4639 893.4606 3.67 0 22 0.99 +1Score > 41 indicates identity
Score > 35 indicates homology		 NALSLGYR + Deamidated (NQ)
1096 473.2813 944.5480 944.5443 3.92 0 22 0.12 +1Score > 38 indicates identity
Score > 25 indicates homology		 IYPGQVLR
1401 536.7876 1071.5606 1071.5672 -6.14 1 22 0.55 +1Score > 42 indicates identity
Score > 32 indicates homology		 VQAAAQRQAK + 2 Deamidated (NQ)
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