MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4026 659.1160 2632.4349 2632.4087 9.95 1 1 1 +1Score > 33 indicates identity
Score > 13 indicates homology
LMKIVFDIIHSGTWNNIDILFK + Oxidation (M)
4170 933.8559 2798.5459 2798.4913 19.5 1 1 1 +1Score > 31 indicates identity
Score > 13 indicates homology
DQDAALTIVTVLDKVAAIVDSVQASQK + Deamidated (NQ)
4406 785.3941 3137.5473 3137.5954 -15.3 1 1 1 +1Score > 36 indicates identity
Score > 13 indicates homology
VNQMLKPTKNLTVNLINIGFDAYADSQK + 3 Deamidated (NQ)
4093 908.1564 2721.4474 2721.4160 11.5 1 1 0.92 +1Score > 35 indicates identity
Score > 13 indicates homology
ILSGIFNGMTDGTPITAIIFNKDQR
4445 641.3645 3201.7861 3201.7954 -2.91 1 1 0.99 +1Score > 29 indicates identity
Score > 13 indicates homology
YITFRTGAALFTSALIVFLFGPTIINSLR
2636 550.9853 1649.9341 1649.9464 -7.50 1 1 1 +1Score > 34 indicates identity
Score > 13 indicates homology
VLLQIHTNDVKTIR + Deamidated (NQ)
4157 697.8798 2787.4901 2787.5072 -6.14 1 1 0.95 +1Score > 34 indicates identity
Score > 13 indicates homology
GQPLGTPQSFGGPYLGIFTVKQQLVR
4290 988.8249 2963.4529 2963.4777 -8.38 1 1 1 +1Score > 37 indicates identity
Score > 13 indicates homology
LINTHQNFVPPNNRFSQQIHVSQLK + 5 Deamidated (NQ)
4659 1011.7505 4042.9729 4042.9249 11.9 1 1 0.97 +1Score > 34 indicates identity
Score > 13 indicates homology
RFSSLHAVLYFSPDFMSLGFMVWASGSMVFFLYR + 3 Oxidation (M)
4265 976.4639 2926.3699 2926.3185 17.6 1 0 1 +1Score > 37 indicates identity
Score > 13 indicates homology
NQFALNSGSMLQNQSQRQNIMDNIR + 4 Deamidated (NQ); Oxidation (M)
1160 970.4799 969.4726 969.4879 -15.8 0 0 1 +1Score > 40 indicates identity
Score > 13 indicates homology
AVAAARPDAE
774 823.4313 822.4240 822.4123 14.2 0 0 0.98 +1Score > 41 indicates identity
Score > 13 indicates homology
QLSSFLE
4181 703.1396 2808.5293 2808.4810 17.2 1 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
IPAQVRPLYQLWRDGQALQLQLAE + 3 Deamidated (NQ)
4287 740.3878 2957.5221 2957.5307 -2.91 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
LEALLNFQQMVIDLTGLPLANASLLDE + Deamidated (NQ); Oxidation (M)
975 904.4822 903.4749 903.4708 4.52 1 0 1 +1Score > 42 indicates identity
Score > 13 indicates homology
KPSRMNR + Oxidation (M)
4011 650.3502 2597.3717 2597.4138 -16.2 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
VGLLVQLANLPQHPESVPVNMLVK + 3 Deamidated (NQ)
4282 985.1932 2952.5578 2952.5040 18.2 0 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
AGVDAASLTSADLAAVTGPDVSPQATALVQR + Deamidated (NQ)
2814 578.6675 1732.9807 1732.9584 12.8 1 0 0.95 +1Score > 34 indicates identity
Score > 13 indicates homology
QQLATAHHTLTLGKSK
4137 925.1373 2772.3901 2772.4123 -8.01 1 0 0.98 +1Score > 37 indicates identity
Score > 13 indicates homology
INGESAAGVVQDLKPPPPPSFSGPPWK + Deamidated (NQ)
4354 1019.2176 3054.6310 3054.6657 -11.4 1 0 1 +1Score > 33 indicates identity
Score > 13 indicates homology
NLLILVFFGMILFIPVQVFSQSRFPE + Deamidated (NQ)
4372 1028.5256 3082.5550 3082.5471 2.54 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
NQLVNSDALLHQQQNHLSLEIINPWR + 3 Deamidated (NQ)
4330 756.0971 3020.3593 3020.3081 17.0 1 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
SDVITNVIDANADTGLDDDPWMKDGTPE + Deamidated (NQ); Oxidation (M)
3410 1060.5602 2119.1058 2119.0918 6.64 1 0 1 +1Score > 38 indicates identity
Score > 13 indicates homology
SPGLMRQLNINGALYVGGMK + Deamidated (NQ)
4772 816.4271 6523.3586 6523.4225 -9.80 1 0 0.97 +1Score > 25 indicates identity
Score > 13 indicates homology
INQVEDRPGGAANVALNIATLGGQVQLAGLVGQDDTAIALTLGVQTLGVEPQWLSIADKPTITK + 5 Deamidated (NQ)
4636 979.4675 3913.8409 3913.8370 0.99 1 0 0.97 +1Score > 33 indicates identity
Score > 13 indicates homology
HLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLE + Deamidated (NQ); Oxidation (M)
4439 799.4266 3193.6773 3193.6594 5.60 1 0 0.94 +1Score > 33 indicates identity
Score > 13 indicates homology
NTRMALAVVDTFVWQSLASVAIPGFTINR + Deamidated (NQ); Oxidation (M)
4443 800.7023 3198.7801 3198.7191 19.1 1 0 1 +1Score > 29 indicates identity
Score > 13 indicates homology
KVAIIFAGAGIVTAPIYLLQHYFYTLMGE
3847 605.3149 2417.2305 2417.2083 9.20 1 0 0.93 +1Score > 38 indicates identity
Score > 13 indicates homology
QVADIILPMLAKQQMHIDSHE + Deamidated (NQ)
4683 826.2094 4126.0106 4126.0602 -12.0 0 0 1 +1Score > 32 indicates identity
Score > 13 indicates homology
NVQPIVSSTHLQQVMEPAVLMQGQEPLTASLLAGAPPQE + 2 Deamidated (NQ); Oxidation (M)
4690 1052.2950 4205.1509 4205.1469 0.96 1 0 1 +1Score > 31 indicates identity
Score > 13 indicates homology
YNVIDTVLALNCAFFVNIAILMLAASVFWKSNIQVTE + 2 Deamidated (NQ); Oxidation (M)
4052 669.3249 2673.2705 2673.3016 -11.6 1 0 1 +1Score > 38 indicates identity
Score > 13 indicates homology
NYTKPRQLCHMSHHLGTIHSVR + 2 Deamidated (NQ)
4721 1175.0962 4696.3557 4696.2649 19.3 0 0 1 +1Score > 30 indicates identity
Score > 13 indicates homology
AAVFGASWNPVHAAGANAVPAAVYHHHHHPYVHPQAPVAAAAPDGR + 3 Deamidated (NQ)
3696 766.7669 2297.2789 2297.2379 17.8 1 0 0.94 +1Score > 33 indicates identity
Score > 13 indicates homology
LQVASIRLGSVFGVHSAIIDSE
3521 546.2836 2181.1053 2181.0670 17.6 1 0 1 +1Score > 39 indicates identity
Score > 13 indicates homology
QGDLQQAMSLYMKANKPAR + 2 Oxidation (M)
4288 988.4935 2962.4587 2962.4027 18.9 1 0 1 +1Score > 37 indicates identity
Score > 13 indicates homology
IITYGKMPYSGMTGAQVIHMLGQNYR + 2 Deamidated (NQ); 2 Oxidation (M)
4774 816.4278 6523.3642 6523.3743 -1.55 1 0 1 +1Score > 25 indicates identity
Score > 13 indicates homology
ALIGVGFLGALTTFSTFSMDSLLLLQQGHFIKMALNIILNVMVCIFMAWLGLQLVMQK + 4 Deamidated (NQ); 4 Oxidation (M)
3927 835.7831 2504.3275 2504.3448 -6.91 0 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
VIQLVALDSQQVMVILVLDTYE + Oxidation (M)
4540 1120.9550 3359.8432 3359.8262 5.05 1 0 0.96 +1Score > 30 indicates identity
Score > 13 indicates homology
ISTPQTNTVPMKPLISTPPVSSQPKVSTPVVK + 2 Deamidated (NQ)
672 775.4270 774.4197 774.4123 9.58 1 0 0.97 +1Score > 43 indicates identity
Score > 13 indicates homology
KNVIATE + Deamidated (NQ)
4634 976.5079 3902.0025 3901.9441 15.0 1 0 1 +1Score > 33 indicates identity
Score > 13 indicates homology
ALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVR + Deamidated (NQ); 2 Oxidation (M)
4739 819.4409 5729.0354 5729.0017 5.88 1 0 1 +1Score > 26 indicates identity
Score > 13 indicates homology
LFGSLGFGVAVFAMGQVTNQLYPIHVIFIFGCAFLCIAAILASQVPGQQKTTK + Deamidated (NQ)
400 327.2038 652.3930 652.4020 -13.8 0 0 1 +1Score > 30 indicates identity
Score > 13 indicates homology
GKPVPR
1553 572.8663 1143.7180 1143.7227 -4.06 1 0 1 +1Score > 27 indicates identity
Score > 13 indicates homology
ITLIGTSLAKK
4753 1194.7882 5968.9046 5968.8856 3.19 1 0 1 +1Score > 27 indicates identity
Score > 13 indicates homology
LAGFKPDIIISGINDGPNLGYDVLYSGTVSAAIEGWMMGYTSIAVSLNSNGQYHFK + 2 Deamidated (NQ); Oxidation (M)
3667 758.7435 2273.2087 2273.1804 12.4 1 0 0.96 +1Score > 37 indicates identity
Score > 13 indicates homology
HNVGFVVADRLAQLAGASFTAK + 2 Deamidated (NQ)
3689 765.4301 2293.2685 2293.2529 6.78 1 0 1 +1Score > 33 indicates identity
Score > 13 indicates homology
KPIEVPAGVTVNVDSNNLVTVK + Deamidated (NQ)
4153 696.3712 2781.4557 2781.4007 19.8 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
NVESIPLMWALSGAGDPDVALNQLVR + Deamidated (NQ); Oxidation (M)
4568 859.4360 3433.7149 3433.7043 3.07 1 0 0.99 +1Score > 35 indicates identity
Score > 13 indicates homology
FVPNNIAMNNLDQQILMITGPNMAGKSTVLR + 2 Deamidated (NQ); 2 Oxidation (M)
4311 998.4980 2992.4722 2992.4384 11.3 1 0 0.98 +1Score > 37 indicates identity
Score > 13 indicates homology
EQISAYGDQIPIVMAPNMAVGVNLMWK + 2 Deamidated (NQ); Oxidation (M)
4351 1018.8349 3053.4829 3053.4362 15.3 1 0 1 +1Score > 37 indicates identity
Score > 13 indicates homology
NVSLQSYVRSISVSSTMGDGCFVDFLAL + 2 Deamidated (NQ)
4639 784.7751 3918.8391 3918.8428 -0.94 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
FPWMNYVSMTAIFLFVSFFEIGPGPIPWFMVAE + Deamidated (NQ); 3 Oxidation (M)
4652 803.3992 4011.9596 4012.0139 -13.5 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
FVSAILQHIRASLNEPQNALYLVLQMLQQATPDSAE + 4 Deamidated (NQ)
3595 741.6849 2222.0329 2222.0195 6.03 1 0 0.98 +1Score > 38 indicates identity
Score > 13 indicates homology
TISVSDLPNNCLNASSLKCE + Deamidated (NQ)
4204 948.5577 2842.6513 2842.6307 7.24 1 0 1 +1Score > 25 indicates identity
Score > 13 indicates homology
ASLVLPFIGKTVDISVSLDLINSLSIK + Deamidated (NQ)
984 909.4601 908.4528 908.4491 4.10 1 0 1 +1Score > 41 indicates identity
Score > 13 indicates homology
ITAIEFSE
2771 572.2845 1713.8317 1713.8243 4.28 0 0 1 +1Score > 40 indicates identity
Score > 13 indicates homology
AQAAPMLAAGDVVQLDE + Oxidation (M)
3700 767.7573 2300.2501 2300.2046 19.8 1 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
NIPVSAGLAGGSTDAAGVLKLMNK + Deamidated (NQ); Oxidation (M)
3754 779.3958 2335.1656 2335.1254 17.2 1 0 1 +1Score > 39 indicates identity
Score > 13 indicates homology
SLSQTFLGGGLDVDMVKVEPNE + Deamidated (NQ)
3823 599.5807 2394.2937 2394.3019 -3.42 1 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
NGIAHLVFNAAGSVNKLNIATLR + 2 Deamidated (NQ)
4701 1115.5759 4458.2745 4458.3003 -5.78 1 0 1 +1Score > 31 indicates identity
Score > 13 indicates homology
SNWLMMHVSVMMLSYSALIVGSLLAIAFLIVTFGQPVELK + 2 Deamidated (NQ); 3 Oxidation (M)
4497 820.4231 3277.6633 3277.6120 15.7 1 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
NYAYLGLKPVFIFFDEYVAFMDLLDMK + Oxidation (M)
4698 868.8597 4339.2621 4339.1803 18.8 1 0 1 +1Score > 30 indicates identity
Score > 13 indicates homology
ASGFAGSMFGSVALGSVCPSLCPPGGIHQVTINKSLLAPLNVE
3884 818.7842 2453.3308 2453.3181 5.16 0 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
FFLLMAVIYTTLAIAPVHFDR + Oxidation (M)
4479 1080.5322 3238.5748 3238.6074 -10.1 0 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
ALNNAGVSGANMLVVLNDNDMSISPPVGALNR + Oxidation (M)
3834 602.3286 2405.2853 2405.2624 9.52 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
NSSVNKMNSVNLSIVFAPNLLK + Deamidated (NQ); Oxidation (M)
4053 892.4530 2674.3372 2674.3553 -6.76 1 0 1 +1Score > 38 indicates identity
Score > 13 indicates homology
RTVLWVIFFMSAVMLFDNWQR + Oxidation (M)
4055 895.7537 2684.2393 2684.2211 6.78 0 0 1 +1Score > 37 indicates identity
Score > 13 indicates homology
LGDIGSLFGVGADAHGAGMHGIDMLQE + Deamidated (NQ); Oxidation (M)
4117 916.5348 2746.5826 2746.5893 -2.45 1 0 0.98 +1Score > 27 indicates identity
Score > 13 indicates homology
SVNGTLKGFLLLLMVIMLAIPFLAR + Deamidated (NQ); Oxidation (M)
3953 842.4677 2524.3813 2524.3974 -6.41 1 0 1 +1Score > 33 indicates identity
Score > 13 indicates homology
IAEINTGIIITPTGQLAMWLGALK + Deamidated (NQ)
4013 651.1201 2600.4513 2600.4061 17.4 1 0 1 +1Score > 31 indicates identity
Score > 13 indicates homology
IDTVLDILVAGDTHSAIHNLEILK + Deamidated (NQ)
3778 590.3234 2357.2645 2357.2750 -4.45 1 0 0.98 +1Score > 36 indicates identity
Score > 13 indicates homology
AAGTAINHSHMLLQRLQDLLR
4318 750.6530 2998.5829 2998.5918 -2.97 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
ASISFNLLSLAGLPPFLGFFIKWMSLE + Deamidated (NQ)
4468 808.8887 3231.5257 3231.5068 5.84 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
GNGEPRVIHINDSLANSSFGYSDNHISTTK + 2 Deamidated (NQ)
4478 810.6469 3238.5585 3238.5088 15.3 0 0 0.99 +1Score > 36 indicates identity
Score > 13 indicates homology
NGNTLNVIVGNGMWFNSSNQLQLDLSGQSK + 4 Deamidated (NQ)
4684 1041.0579 4160.2025 4160.1461 13.6 1 0 1 +1Score > 31 indicates identity
Score > 13 indicates homology
QYMPDDLDMILTSVITLLIMGAVTFLIIMPIGGELFK + Deamidated (NQ); 3 Oxidation (M)
4741 1149.0293 5740.1101 5740.0285 14.2 1 0 1 +1Score > 26 indicates identity
Score > 13 indicates homology
FSSILASGQGSIMQLGIGPIVTASIVLQLLGGADLLGLNTQDDPRDQILYQGLQK + 2 Deamidated (NQ); Oxidation (M)
4455 1070.5636 3208.6690 3208.6705 -0.48 0 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
DIIALGFMTFALFVGAGNIIFPPMVGLQAGE
4600 908.7581 3631.0033 3631.0132 -2.74 0 0 1 +1Score > 28 indicates identity
Score > 13 indicates homology
DILLLLLGLIVLVNIGINVATMMWHGLQNALDK + 2 Deamidated (NQ); Oxidation (M)
2700 335.6097 1673.0121 1672.9849 16.3 1 0 1 +1Score > 26 indicates identity
Score > 13 indicates homology
LRLLGPGAARPASPAAR
3378 1055.5327 2109.0508 2109.0412 4.58 1 0 1 +1Score > 39 indicates identity
Score > 13 indicates homology
LLGEALGLSSDQIGMAYQSR + Deamidated (NQ)
4366 769.4334 3073.7045 3073.6560 15.8 1 0 1 +1Score > 30 indicates identity
Score > 13 indicates homology
NQPTLLRLINSSAQAQFQYQQLTAIVK + Deamidated (NQ)
3524 728.0615 2181.1627 2181.1794 -7.65 1 0 1 +1Score > 37 indicates identity
Score > 13 indicates homology
VNGQRVNIPSFLVKPGDVIE + Deamidated (NQ)
4384 1033.5526 3097.6360 3097.6079 9.05 1 0 1 +1Score > 33 indicates identity
Score > 13 indicates homology
LLVPGTYNLTALSTGYMPLTINNIMVKE + 2 Oxidation (M)
4320 1002.1170 3003.3292 3003.3457 -5.51 0 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
VSAGNSVLWSCGQQVQSNPSASWSYFR + 2 Deamidated (NQ)
4710 1138.6033 4550.3841 4550.3373 10.3 1 0 1 +1Score > 29 indicates identity
Score > 13 indicates homology
YGDIQAVSLSEIVQHPPFDLIINATSLGLQGGYIALPNHLFE + Deamidated (NQ)
3386 704.3742 2110.1008 2110.0794 10.1 1 0 1 +1Score > 38 indicates identity
Score > 13 indicates homology
ILSDLEVNLGDVANGLDQPK + Deamidated (NQ)
4541 673.3791 3361.8591 3361.8474 3.50 1 0 1 +1Score > 29 indicates identity
Score > 13 indicates homology
NVYIMLLLISIIWMGLIGFIDDYIKVFK + 2 Oxidation (M)
1953 666.3922 1330.7698 1330.7510 14.2 1 0 1 +1Score > 35 indicates identity
Score > 13 indicates homology
FPTVPIFGRAAR
4680 1030.7928 4119.1421 4119.0960 11.2 1 0 1 +1Score > 31 indicates identity
Score > 13 indicates homology
WSHSGSSTARLLLIGVLFASCSTAILGAQRPPINSAGGHE + Deamidated (NQ)
4304 997.5184 2989.5334 2989.5291 1.42 1 0 1 +1Score > 36 indicates identity
Score > 13 indicates homology
DQLQSVLGTAGHKHLMALQSSPSSGVVDK
4470 809.1932 3232.7437 3232.6836 18.6 0 0 1 +1Score > 31 indicates identity
Score > 13 indicates homology
DQHMISQLIGTIFTCVGITTLIQTTVGIR + Deamidated (NQ); Oxidation (M)
1002 916.4735 915.4662 915.4563 10.9 1 0 1 +1Score > 42 indicates identity
Score > 13 indicates homology
SAFIEGHR
4565 1144.5961 3430.7665 3430.7079 17.1 1 0 1 +1Score > 34 indicates identity
Score > 13 indicates homology
FSIAALRGEPVDSYGVGTSLVTGSGAPTANMVYK + Oxidation (M)
1 301.9112 300.9039
2 302.1780 301.1707
3 303.0619 302.0546
4 303.1784 302.1711
5 303.1785 302.1712
6 303.1786 302.1713
7 303.1788 302.1715
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