MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4159 558.7022 2788.4746 2788.4568 6.38 0 2 0.75 +1Score > 35 indicates identity
Score > 13 indicates homology
IIMLGTAIDDNVANSIVAQLLFLQAE + Deamidated (NQ); Oxidation (M)
4218 718.3855 2869.5129 2869.4614 18.0 0 2 0.75 +1Score > 35 indicates identity
Score > 13 indicates homology
SALGMAIVMLLMGMAQNIWQFLILR + 2 Deamidated (NQ); 3 Oxidation (M)
3970 852.1124 2553.3154 2553.2697 17.9 0 2 1 +1Score > 37 indicates identity
Score > 14 indicates homology
DDAIIILDPVNQDVITDGLNNGVK + 3 Deamidated (NQ)
2313 754.9254 1507.8362 1507.8069 19.5 1 2 0.83 +1Score > 37 indicates identity
Score > 13 indicates homology
VGIATFLVGESLMR + Oxidation (M)
2867 591.9647 1772.8723 1772.8846 -6.94 1 2 1 +1Score > 40 indicates identity
Score > 14 indicates homology
LWQYTLAEAHDLTGR
848 852.5742 851.5669 851.5593 9.01 0 2 1 +1Score > 24 indicates identity
Score > 14 indicates homology
VIAIIPAR
4323 1004.1929 3009.5569 3009.5456 3.75 1 2 0.85 +1Score > 35 indicates identity
Score > 13 indicates homology
GIKTHAPNAFVICITNPLDAMVWALQK + 2 Deamidated (NQ)
3630 749.7452 2246.2138 2246.1776 16.1 1 2 1 +1Score > 35 indicates identity
Score > 14 indicates homology
AVTHAIVGMLPHNSLGRQMAK + Oxidation (M)
4309 998.4959 2992.4659 2992.4272 12.9 1 2 1 +1Score > 37 indicates identity
Score > 14 indicates homology
IQINAFAEQTPVVMAPNMSVGVNLMWK + 5 Deamidated (NQ)
2911 901.5062 1800.9978 1800.9655 17.9 1 2 1 +1Score > 36 indicates identity
Score > 14 indicates homology
LVVLGGAMGATTLNAEGVK + Deamidated (NQ)
4043 885.4592 2653.3558 2653.3996 -16.5 1 2 1 +1Score > 37 indicates identity
Score > 14 indicates homology
VTINQSLLTPMNLQIDPTIQRVR + 4 Deamidated (NQ)
3459 716.3770 2146.1092 2146.0827 12.3 1 2 0.84 +1Score > 38 indicates identity
Score > 13 indicates homology
DASLKALNNLDILGLNMDAK + 2 Deamidated (NQ); Oxidation (M)
3820 797.4386 2389.2940 2389.2709 9.67 1 2 0.96 +1Score > 35 indicates identity
Score > 14 indicates homology
MNPVISITLLLSVLQMSRGQR + 2 Deamidated (NQ); 2 Oxidation (M)
4124 918.8073 2753.4001 2753.4132 -4.77 1 2 0.97 +1Score > 37 indicates identity
Score > 14 indicates homology
NPLAAVMMGLIYVNPEGVDGKPDPLK + Oxidation (M)
4449 802.4233 3205.6641 3205.6562 2.46 1 2 1 +1Score > 35 indicates identity
Score > 14 indicates homology
ILVQILQCWIQDVPFLHFLQFFLRE + 4 Deamidated (NQ)
4735 887.4508 5318.6611 5318.6155 8.57 0 2 1 +1Score > 29 indicates identity
Score > 14 indicates homology
YPSYMNNNLIGLTAPLIPTPQLHFLMTGYTPLTSDSDIHTQQLVNVR + 3 Deamidated (NQ); 2 Oxidation (M)
3471 1076.5587 2151.1028 2151.0848 8.41 1 2 0.89 +1Score > 38 indicates identity
Score > 14 indicates homology
KPHTTTVAPPQINFIKNNE + 3 Deamidated (NQ)
758 814.4003 813.3930 813.3981 -6.19 0 2 1 +1Score > 39 indicates identity
Score > 14 indicates homology
AVQGNAPR + 2 Deamidated (NQ)
2915 601.6091 1801.8055 1801.8105 -2.81 0 2 0.98 +1Score > 38 indicates identity
Score > 14 indicates homology
GISLDNTTLSSTQTFSE + 2 Deamidated (NQ)
3130 650.3806 1948.1200 1948.1431 -11.9 1 2 0.94 +1Score > 30 indicates identity
Score > 14 indicates homology
AIIGSVVPNLMPTIPKAVK + Deamidated (NQ)
4594 903.4889 3609.9265 3609.8865 11.1 1 1 1 +1Score > 32 indicates identity
Score > 14 indicates homology
NTLPQLAATIIALSLQGVCLGQRNLPPPDYFTE
4274 978.8447 2933.5123 2933.4732 13.3 1 1 0.85 +1Score > 36 indicates identity
Score > 13 indicates homology
LAQGLVGQPLLMPVAVPVDDNWNEVIE + 2 Deamidated (NQ); Oxidation (M)
2093 698.3785 1394.7424 1394.7630 -14.7 1 1 0.91 +1Score > 39 indicates identity
Score > 14 indicates homology
DKNHLLASASAIR
4583 891.4622 3561.8197 3561.8801 -17.0 0 1 1 +1Score > 34 indicates identity
Score > 14 indicates homology
TMTLLIFFFFLYYITSLLVAFSYLITNYK + Deamidated (NQ); Oxidation (M)
3607 744.7487 2231.2243 2231.1806 19.6 1 1 1 +1Score > 34 indicates identity
Score > 14 indicates homology
VTGNMRAILIDWLVQVQMK + Deamidated (NQ); Oxidation (M)
3671 1139.5819 2277.1492 2277.1311 7.98 1 1 0.81 +1Score > 39 indicates identity
Score > 13 indicates homology
LCSFIAQGQNRSPLIQTLTQ + 3 Deamidated (NQ)
4421 529.9227 3173.4925 3173.4883 1.33 1 1 0.82 +1Score > 36 indicates identity
Score > 13 indicates homology
SGFWNFDALFVPQQHSARDAQDTFFLK + 2 Deamidated (NQ)
4260 973.8676 2918.5810 2918.5423 13.3 1 1 0.81 +1Score > 33 indicates identity
Score > 13 indicates homology
ILDKTAPMASDLNVQVPGPTVSPKPLGR + 2 Deamidated (NQ); Oxidation (M)
4306 748.4000 2989.5709 2989.5742 -1.12 1 1 1 +1Score > 34 indicates identity
Score > 14 indicates homology
LLWHGTNMAVVAAILTSGLRIMPHSGGR + 2 Oxidation (M)
4348 1015.8792 3044.6158 3044.5940 7.16 1 1 1 +1Score > 34 indicates identity
Score > 14 indicates homology
ALIGIVIGGFWSMSAATAMRLVPANDVPR + 2 Oxidation (M)
3283 681.0325 2040.0757 2040.0640 5.73 1 1 0.76 +1Score > 38 indicates identity
Score > 13 indicates homology
AISPGNGAKVNLIAPDDFNK
4272 978.8281 2933.4625 2933.5096 -16.1 1 1 0.97 +1Score > 37 indicates identity
Score > 14 indicates homology
TGVFTNTAGLQKLMDIIQIVFSVDPPE + Deamidated (NQ)
4643 792.0314 3955.1206 3955.0587 15.7 1 1 1 +1Score > 30 indicates identity
Score > 14 indicates homology
GSPQHQRNVLIVLGLFGAALLYGDGMITPAISVLSAME + Deamidated (NQ); Oxidation (M)
2850 882.9756 1763.9366 1763.9128 13.5 1 1 0.98 +1Score > 39 indicates identity
Score > 14 indicates homology
TGLFTLQSELMVTPAR + Deamidated (NQ)
3315 1030.5450 2059.0754 2059.0626 6.24 1 1 1 +1Score > 38 indicates identity
Score > 14 indicates homology
QLEANLGYQLPVDFVPVR + 2 Deamidated (NQ)
3331 693.4196 2077.2370 2077.2074 14.2 1 1 1 +1Score > 25 indicates identity
Score > 14 indicates homology
FINILSNLLQKGTLIFIK + 3 Deamidated (NQ)
3780 787.4255 2359.2547 2359.2787 -10.2 1 1 0.76 +1Score > 36 indicates identity
Score > 13 indicates homology
LLPPALYSRLAGEPGAAEPLPVE
4700 887.6166 4433.0466 4433.0113 7.97 1 1 1 +1Score > 31 indicates identity
Score > 14 indicates homology
NGYAYHGVALNVDMDLSPFLGINPCGYEGLVTVDMAACGVR + 2 Deamidated (NQ); Oxidation (M)
3254 677.0103 2028.0091 2028.0058 1.60 1 1 0.77 +1Score > 40 indicates identity
Score > 13 indicates homology
VTMVSLISGAANNGELSHGR + Oxidation (M)
4405 1046.5663 3136.6771 3136.6366 12.9 1 1 0.99 +1Score > 33 indicates identity
Score > 14 indicates homology
NYLKQIQIIPIQPDMAVAILVTNTGHVE + 3 Deamidated (NQ); Oxidation (M)
4448 1068.8607 3203.5603 3203.6179 -18.0 1 1 0.8 +1Score > 36 indicates identity
Score > 13 indicates homology
MVGSNVAGASLGAALDGMLTTAGIAGRWVQAAR + 2 Oxidation (M)
1357 1053.5304 1052.5231 1052.5107 11.8 1 1 1 +1Score > 41 indicates identity
Score > 14 indicates homology
KCLSVGMSR + Oxidation (M)
4191 706.3456 2821.3533 2821.3527 0.21 1 1 1 +1Score > 37 indicates identity
Score > 14 indicates homology
RQQAALCAQHALNMLLQDSLFTYE + Deamidated (NQ)
4556 681.9844 3404.8856 3404.9084 -6.70 1 1 1 +1Score > 28 indicates identity
Score > 14 indicates homology
AWLLFAHQGAEPGHKTLLAALQAEPLLALGLR
3956 844.4328 2530.2766 2530.3206 -17.4 0 1 0.92 +1Score > 38 indicates identity
Score > 13 indicates homology
TLAPNPSLLLLDEPFSALDFQTK + Deamidated (NQ)
4526 1111.5938 3331.7596 3331.8014 -12.6 0 1 1 +1Score > 33 indicates identity
Score > 14 indicates homology
NSVVALIPAIILSDGVVTLDGVPAISDVDNLIE + Deamidated (NQ)
1629 591.3667 1180.7188 1180.7404 -18.2 1 1 1 +1Score > 32 indicates identity
Score > 14 indicates homology
KARPQLILSR
3526 728.3784 2182.1134 2182.1277 -6.55 1 1 1 +1Score > 39 indicates identity
Score > 14 indicates homology
ATRPVQMPGASLASAPTARGSR + Deamidated (NQ)
900 872.4522 871.4449 871.4399 5.72 1 1 0.77 +1Score > 40 indicates identity
Score > 13 indicates homology
SPIEGVDR
3572 736.3896 2206.1470 2206.1746 -12.5 1 1 0.82 +1Score > 38 indicates identity
Score > 13 indicates homology
ADNINWANLIEKPITPLQR + Deamidated (NQ)
3075 634.0179 1899.0319 1899.0313 0.31 1 1 1 +1Score > 37 indicates identity
Score > 14 indicates homology
NVRGILADIGVSSLQLDK + 2 Deamidated (NQ)
3875 814.3987 2440.1743 2440.2097 -14.5 1 1 0.81 +1Score > 38 indicates identity
Score > 13 indicates homology
IADGVVRFVFTTGMHALAYVQE + Deamidated (NQ); Oxidation (M)
4564 687.1553 3430.7401 3430.7896 -14.4 0 1 0.92 +1Score > 35 indicates identity
Score > 13 indicates homology
AIDVMNPSLGWMIGFMFVVSFLGLFSLVALR + Deamidated (NQ)
4615 744.9982 3719.9546 3719.9439 2.88 1 1 0.84 +1Score > 33 indicates identity
Score > 13 indicates homology
AAAQMISYEIPLVMSALGVVLLAGSMNLVDIVAAQK + 2 Deamidated (NQ); 2 Oxidation (M)
4703 901.1160 4500.5436 4500.4788 14.4 1 1 1 +1Score > 22 indicates identity
Score > 14 indicates homology
VPYIIIGQLATIFYFSYFMILLPLISILENMLFYLAIK + Deamidated (NQ); Oxidation (M)
3136 653.0334 1956.0784 1956.0502 14.4 0 1 0.9 +1Score > 35 indicates identity
Score > 13 indicates homology
NYLIPQGLAMLATPANLR + Deamidated (NQ)
3413 708.0319 2121.0739 2121.1140 -18.9 1 1 0.77 +1Score > 39 indicates identity
Score > 13 indicates homology
DTYRVLTAVDAAVMVIDAAK
2709 843.9457 1685.8768 1685.8526 14.4 0 1 1 +1Score > 40 indicates identity
Score > 14 indicates homology
FADTLGVLDPFQHAR
3339 696.0178 2085.0316 2084.9936 18.2 1 1 1 +1Score > 39 indicates identity
Score > 14 indicates homology
MLHTSITVPLSAENVDVNE + Deamidated (NQ); Oxidation (M)
3496 543.5516 2170.1773 2170.1858 -3.93 1 1 0.91 +1Score > 36 indicates identity
Score > 13 indicates homology
DLVHGGAHKLNNTLGQALLAK + Deamidated (NQ)
4005 647.3450 2585.3509 2585.4019 -19.7 1 1 0.98 +1Score > 36 indicates identity
Score > 14 indicates homology
WSIATAIASTAKGFLHLHHHFLK
4035 661.0840 2640.3069 2640.3143 -2.82 1 1 0.98 +1Score > 38 indicates identity
Score > 14 indicates homology
SSAAQPGVAFSVDNTIKRPNPAPGTR + 3 Deamidated (NQ)
3172 661.6758 1982.0056 1982.0010 2.31 0 1 1 +1Score > 39 indicates identity
Score > 14 indicates homology
VNFAVQAFAALNSNNFVR + Deamidated (NQ)
4149 696.0990 2780.3669 2780.3804 -4.84 1 1 0.83 +1Score > 38 indicates identity
Score > 13 indicates homology
AAVPITGVLGDQHAATVGQVCFSPGEAK + Deamidated (NQ)
3688 765.4299 2293.2679 2293.2529 6.52 1 1 1 +1Score > 33 indicates identity
Score > 14 indicates homology
KPIEVPAGVTVNVDSNNLVTVK + Deamidated (NQ)
4473 1079.5653 3235.6741 3235.6765 -0.75 1 1 1 +1Score > 34 indicates identity
Score > 14 indicates homology
AVRASAITDTDGLLTFSAADQPWVFQTLLK + Deamidated (NQ)
2619 820.9631 1639.9116 1639.8907 12.8 1 1 1 +1Score > 37 indicates identity
Score > 14 indicates homology
EPLVVAAKHHVNGNR
3846 484.4529 2417.2281 2417.1944 14.0 0 1 0.94 +1Score > 38 indicates identity
Score > 13 indicates homology
VWYFLLTGGVALDNPFPNPAPE + Deamidated (NQ)
4021 876.7498 2627.2276 2627.2207 2.62 1 1 0.99 +1Score > 37 indicates identity
Score > 14 indicates homology
MNRTFNNGIGMVVVVDAANAQATAK + 4 Deamidated (NQ); 2 Oxidation (M)
4195 942.8179 2825.4319 2825.4242 2.71 1 1 0.97 +1Score > 37 indicates identity
Score > 14 indicates homology
SLITINNVSPLNCVRWRPANQQSR + 3 Deamidated (NQ)
4367 770.4224 3077.6605 3077.6848 -7.89 1 1 0.8 +1Score > 32 indicates identity
Score > 13 indicates homology
IAVAQYTQAMAARAEPSIHFNPILLKPK
4428 1061.5262 3181.5568 3181.5682 -3.59 1 1 1 +1Score > 36 indicates identity
Score > 14 indicates homology
LAMHGTHNMLNSLAAIGVATQMGIADDKIR + Deamidated (NQ); 2 Oxidation (M)
3808 794.4384 2380.2934 2380.3114 -7.57 1 1 0.91 +1Score > 34 indicates identity
Score > 13 indicates homology
LQFLSGAIENPQRPLAAIIGGSK + Deamidated (NQ)
1048 929.4828 928.4755 928.4865 -11.8 0 1 0.95 +1Score > 40 indicates identity
Score > 14 indicates homology
ALLLQGANE + Deamidated (NQ)
3519 727.0826 2178.2260 2178.2008 11.6 1 1 1 +1Score > 32 indicates identity
Score > 14 indicates homology
LIQQLQQQALSEPAVVQKR + 2 Deamidated (NQ)
3813 796.3977 2386.1713 2386.1297 17.4 0 1 0.86 +1Score > 39 indicates identity
Score > 13 indicates homology
DQPPPLSLMPSNPTQMLHPPR + 2 Deamidated (NQ); 2 Oxidation (M)
3893 823.1332 2466.3778 2466.3305 19.2 1 1 1 +1Score > 31 indicates identity
Score > 14 indicates homology
EPNVNPTRLPFIIMLVGVQGSGK + Deamidated (NQ)
4150 557.0807 2780.3671 2780.3651 0.74 1 1 0.99 +1Score > 38 indicates identity
Score > 14 indicates homology
GATGPEGVLPRPLLSCTQALDDDLQR + 2 Deamidated (NQ)
4079 676.6384 2702.5245 2702.4894 13.0 1 1 1 +1Score > 29 indicates identity
Score > 14 indicates homology
VLAYLASLTDAPLLYGGSVKPANIKE
4134 692.3432 2765.3437 2765.3020 15.1 1 1 1 +1Score > 38 indicates identity
Score > 14 indicates homology
ESLFAWMHQNGAKPSDFFSIPANR + Oxidation (M)
3255 677.3657 2029.0753 2029.0567 9.13 0 1 0.82 +1Score > 38 indicates identity
Score > 13 indicates homology
ALWQAFLDAGVKPCGLGAR
4192 941.4794 2821.4164 2821.3956 7.36 1 1 1 +1Score > 37 indicates identity
Score > 14 indicates homology
DNAVRLMQSTPVIDGHNDLPWALLK + 3 Deamidated (NQ); Oxidation (M)
4377 1031.5326 3091.5760 3091.6052 -9.47 1 1 0.94 +1Score > 36 indicates identity
Score > 13 indicates homology
YAQVKPDGTYVKPLSNKLTYGTMVFVR + Deamidated (NQ); Oxidation (M)
3168 661.3100 1980.9082 1980.9431 -17.6 1 1 0.98 +1Score > 39 indicates identity
Score > 14 indicates homology
SMGSKLSPGAQQLMDMIR + 2 Oxidation (M)
3536 730.3834 2188.1284 2188.0994 13.3 1 1 0.99 +1Score > 38 indicates identity
Score > 14 indicates homology
MASPRHPVSAHAVALVQMDR + Oxidation (M)
3909 622.8283 2487.2841 2487.3117 -11.1 0 1 0.82 +1Score > 37 indicates identity
Score > 13 indicates homology
ILLAIALMLSTVMWVSTQQPQE + Oxidation (M)
1350 1046.5503 1045.5430 1045.5444 -1.32 1 1 0.83 +1Score > 42 indicates identity
Score > 13 indicates homology
QDIPEGLFK
4489 652.9694 3259.8106 3259.7939 5.14 1 1 1 +1Score > 28 indicates identity
Score > 14 indicates homology
MLMILLFQRLGIIMILAFLLVNNSYFR + 2 Deamidated (NQ); Oxidation (M)
4209 954.1403 2859.3991 2859.4476 -17.0 1 1 1 +1Score > 38 indicates identity
Score > 14 indicates homology
SIGMIFQHFNLLSSRTAADNIALPLE + 2 Deamidated (NQ)
3279 510.7732 2039.0637 2039.0299 16.6 1 1 0.92 +1Score > 38 indicates identity
Score > 13 indicates homology
GLRVPHAIWPDMVYAGLE + Oxidation (M)
3790 790.7967 2369.3683 2369.4055 -15.7 1 1 1 +1Score > 28 indicates identity
Score > 14 indicates homology
AILVMIMVVIALANRYVLVPR + Oxidation (M)
4256 971.4399 2911.2979 2911.3393 -14.2 1 1 1 +1Score > 35 indicates identity
Score > 14 indicates homology
TLQSGVNMFDIDVLNGLIQESGNTSNQ + 2 Deamidated (NQ); Oxidation (M)
3464 716.7732 2147.2978 2147.2565 19.2 1 1 0.88 +1Score > 22 indicates identity
Score > 13 indicates homology
LQDALASLPDAPALVVIKVSK
4369 770.8929 3079.5425 3079.5132 9.53 1 1 0.93 +1Score > 36 indicates identity
Score > 13 indicates homology
SRYNVDVAQLPGADLSQLTSAQAASLMQK + 2 Deamidated (NQ); Oxidation (M)
4082 676.8580 2703.4029 2703.3935 3.48 1 1 0.8 +1Score > 36 indicates identity
Score > 13 indicates homology
SKCSIDGILLLNKPQGMTSNAALQK + Deamidated (NQ); Oxidation (M)
4322 1004.1920 3009.5542 3009.5198 11.4 1 1 0.94 +1Score > 35 indicates identity
Score > 13 indicates homology
HQILIGNMMSSRSPVDSLILMHGVGTGK + 2 Oxidation (M)
4396 1043.9355 3128.7847 3128.7307 17.2 1 1 1 +1Score > 27 indicates identity
Score > 14 indicates homology
VQILSVTALGNLDAYIRIANLWPMLSIK + Deamidated (NQ); Oxidation (M)
4484 651.9650 3254.7886 3254.7245 19.7 1 1 1 +1Score > 30 indicates identity
Score > 14 indicates homology
SGSLDIQVSKLGQDSVLAQIIQLVQQAQASK + 3 Deamidated (NQ)
3773 786.4217 2356.2433 2356.1984 19.0 1 1 0.9 +1Score > 37 indicates identity
Score > 13 indicates homology
QPAEVIPQQMIGFSDPAVSTLK + Deamidated (NQ)
4595 723.3875 3611.9011 3611.8797 5.94 1 1 1 +1Score > 32 indicates identity
Score > 14 indicates homology
VQNQAMDATLPAHAPTIPLVPATDAPLVGLAFKLE + 3 Deamidated (NQ)
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