MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3145 654.9879 1961.9419 1961.9807 -19.8 1 3 0.77 +1Score > 40 indicates identity
Score > 14 indicates homology
QRASAGQISVQPTFSVQR + 3 Deamidated (NQ)
4028 659.8136 2635.2253 2635.2581 -12.4 1 3 0.84 +1Score > 37 indicates identity
Score > 15 indicates homology
LFPQFSKYQLSQTLLAYDNNIE + 4 Deamidated (NQ)
3706 1151.6401 2301.2656 2301.2216 19.1 0 3 0.82 +1Score > 34 indicates identity
Score > 14 indicates homology
ASQFAAIASALLINVDTLTQPR + 2 Deamidated (NQ)
4032 659.8154 2635.2325 2635.2725 -15.2 1 3 0.99 +1Score > 37 indicates identity
Score > 15 indicates homology
AQAQATSAGNAEIDPLHVLSAAVNQR + 4 Deamidated (NQ)
3449 715.3694 2143.0864 2143.1008 -6.74 1 3 0.82 +1Score > 39 indicates identity
Score > 14 indicates homology
VLANSDVDIIAAESLTDAAQK
3588 740.3928 2218.1566 2218.1456 4.94 1 3 1 +1Score > 38 indicates identity
Score > 15 indicates homology
KVMAILLHGDAAFAGQGIVYE + Oxidation (M)
1851 640.8893 1279.7640 1279.7612 2.23 1 3 0.65 +1Score > 33 indicates identity
Score > 13 indicates homology
IAGPIPKTNLTR
4392 1040.5537 3118.6393 3118.6382 0.35 1 3 0.67 +1Score > 34 indicates identity
Score > 14 indicates homology
LKFVILVCSVACVFGAVVPGGMLPQLDGR + Deamidated (NQ); Oxidation (M)
4531 1114.9415 3341.8027 3341.8210 -5.47 1 3 0.64 +1Score > 31 indicates identity
Score > 13 indicates homology
LNHNHMLLWLLLIGTVPVGIAGLLFNKQAE + 2 Deamidated (NQ); Oxidation (M)
3023 933.5021 1864.9896 1864.9757 7.47 1 3 0.66 +1Score > 38 indicates identity
Score > 13 indicates homology
DPESMVLFGVGALLFVR + Oxidation (M)
3829 800.4465 2398.3177 2398.3294 -4.88 0 3 0.61 +1Score > 34 indicates identity
Score > 13 indicates homology
GPTPISALIQAATMVAAGIFLLAR + Deamidated (NQ); Oxidation (M)
4300 746.8965 2983.5569 2983.5266 10.1 0 3 0.6 +1Score > 35 indicates identity
Score > 13 indicates homology
GQIAFYTGTDPTGPSLHIGHMVPIFALK + Oxidation (M)
4312 998.4985 2992.4737 2992.5256 -17.3 1 3 0.98 +1Score > 37 indicates identity
Score > 15 indicates homology
FIPGMLTNPNLSNYLEVDLLFVVDPR + Deamidated (NQ); Oxidation (M)
2535 806.4175 1610.8204 1610.8264 -3.70 0 3 1 +1Score > 40 indicates identity
Score > 15 indicates homology
TDPGVSAVGNAVLAPSR + Deamidated (NQ)
4139 925.8431 2774.5075 2774.4814 9.39 0 3 1 +1Score > 33 indicates identity
Score > 15 indicates homology
AGQTVIVSGLNPAAILQSTIGGATPAPAAE
3113 645.3254 1932.9544 1932.9265 14.4 1 3 0.62 +1Score > 40 indicates identity
Score > 13 indicates homology
QMFNRFLHDPTQNLR + Deamidated (NQ); Oxidation (M)
4047 666.8476 2663.3613 2663.3411 7.58 0 3 0.81 +1Score > 37 indicates identity
Score > 14 indicates homology
AIQTLVTAMHLVQTNHQIVQMAAE + Deamidated (NQ); Oxidation (M)
4378 773.9227 3091.6617 3091.6039 18.7 1 3 0.61 +1Score > 33 indicates identity
Score > 13 indicates homology
TTVLATVVSMKEPKPGIDFLPLTVNYQE + 2 Deamidated (NQ)
3584 738.3621 2212.0645 2212.0722 -3.48 0 3 0.88 +1Score > 39 indicates identity
Score > 15 indicates homology
VAGNVNIIWPDSNPTIEPMK + 2 Deamidated (NQ); Oxidation (M)
4441 799.6516 3194.5773 3194.5335 13.7 1 3 1 +1Score > 36 indicates identity
Score > 15 indicates homology
VPNLNIPNNYISYIYSDYYIQAKIGQE + 3 Deamidated (NQ)
4747 1165.4225 5822.0761 5822.0526 4.04 1 3 1 +1Score > 25 indicates identity
Score > 15 indicates homology
SPVVDSAVATLSSPSIAVFGGVSFKTNGGLPSGMPLTSILNSLNHCLLVGSAIIQVLE + Deamidated (NQ)
1782 621.3610 1240.7074 1240.6888 15.1 1 3 0.87 +1Score > 38 indicates identity
Score > 15 indicates homology
KGSISNASPKPR
3864 809.4406 2425.3000 2425.3450 -18.6 1 3 0.96 +1Score > 35 indicates identity
Score > 15 indicates homology
RVAMMGIGGAAIIGLIAPNNALFR
4485 1086.5720 3256.6942 3256.7391 -13.8 1 3 0.72 +1Score > 34 indicates identity
Score > 14 indicates homology
DIVVGTPVAGRVHDDLQHIIGMFVNTLAIR + Deamidated (NQ)
716 792.3986 791.3913 791.4025 -14.1 0 3 0.75 +1Score > 42 indicates identity
Score > 14 indicates homology
IDATGTSK
2226 489.6346 1465.8820 1465.8769 3.46 0 3 1 +1Score > 30 indicates identity
Score > 15 indicates homology
HLLGISLQFALVR
2788 432.4744 1725.8685 1725.8897 -12.3 1 3 0.86 +1Score > 40 indicates identity
Score > 14 indicates homology
NAAVLINNQKEPVGTR + 3 Deamidated (NQ)
4298 746.4337 2981.7057 2981.7504 -15.0 1 3 0.93 +1Score > 26 indicates identity
Score > 15 indicates homology
LISPQIMGKNLHIHPITIIFLLLTAGK + Deamidated (NQ)
4778 1175.6140 7047.6403 7047.5237 16.5 1 3 0.69 +1Score > 25 indicates identity
Score > 14 indicates homology
TRLLGHLANCMTQINAMTYPGQAHPALQAPPPPPPSGPAGPQHAPFAPPPPPLVPIPGGAAPPPGSAPCK + 2 Deamidated (NQ); Oxidation (M)
981 907.4651 906.4578 906.4559 2.10 1 3 0.64 +1Score > 42 indicates identity
Score > 13 indicates homology
QRDNFVK + Deamidated (NQ)
3602 744.3751 2230.1035 2230.1403 -16.5 1 3 1 +1Score > 39 indicates identity
Score > 15 indicates homology
GLDSLNDDQLVMLKLTIPTE + Oxidation (M)
3099 640.0093 1917.0061 1917.0030 1.62 1 3 0.61 +1Score > 38 indicates identity
Score > 13 indicates homology
QIPELPDPNLDMGILPR
3540 731.3876 2191.1410 2191.1790 -17.3 1 3 0.67 +1Score > 38 indicates identity
Score > 13 indicates homology
VQYALEGSVFVGGAVIQWIR
4228 959.8413 2876.5021 2876.4767 8.82 1 3 0.75 +1Score > 35 indicates identity
Score > 14 indicates homology
QTLVLNPRGVNPTNGVASLSQAGAVPALE + 4 Deamidated (NQ)
4453 1070.5582 3208.6528 3208.6430 3.03 1 3 1 +1Score > 34 indicates identity
Score > 15 indicates homology
SSANGNLLVYQSPVIKSNINPIYNPIIFK + 6 Deamidated (NQ)
2107 701.4038 1400.7930 1400.7697 16.6 0 3 0.9 +1Score > 37 indicates identity
Score > 15 indicates homology
MTAPILVATLDTR
2569 809.4703 1616.9260 1616.9072 11.7 1 3 1 +1Score > 33 indicates identity
Score > 15 indicates homology
QVMVFKPSNVLKAR + Deamidated (NQ)
2488 794.4052 1586.7958 1586.7827 8.26 0 3 0.59 +1Score > 41 indicates identity
Score > 13 indicates homology
SAFNSSYLLSALANK + 2 Deamidated (NQ)
3734 770.7764 2309.3074 2309.2664 17.7 1 3 1 +1Score > 30 indicates identity
Score > 15 indicates homology
TIDHLIILIQMVQKGTVNQK + 2 Deamidated (NQ); Oxidation (M)
3748 776.0757 2325.2053 2325.1753 12.9 1 3 0.65 +1Score > 38 indicates identity
Score > 13 indicates homology
QHPGSFKYTAVTDTPNLLHAK + Deamidated (NQ)
3763 782.3589 2344.0549 2344.0774 -9.61 1 3 0.64 +1Score > 37 indicates identity
Score > 13 indicates homology
QMSLMALADTIATTSTDIGESR + Deamidated (NQ); 2 Oxidation (M)
3792 475.4759 2372.3431 2372.3355 3.20 0 3 0.96 +1Score > 30 indicates identity
Score > 15 indicates homology
IAAFQALDLDAAVVVAFGQILVK + Deamidated (NQ)
2236 736.3688 1470.7230 1470.7177 3.63 0 3 0.89 +1Score > 41 indicates identity
Score > 15 indicates homology
IGLPAGMFHVDGLE + Oxidation (M)
2462 787.9844 1573.9542 1573.9304 15.2 1 3 1 +1Score > 26 indicates identity
Score > 15 indicates homology
QAALVLYRSLLTAR
3245 1012.5303 2023.0460 2023.0698 -11.7 1 3 1 +1Score > 39 indicates identity
Score > 15 indicates homology
ANEGVVIIAATNRPDVLDR + Deamidated (NQ)
4389 624.1034 3115.4806 3115.4296 16.4 1 3 0.99 +1Score > 36 indicates identity
Score > 15 indicates homology
LTLTLSCPMDLKNFPMDVQTCIMQLE + 2 Deamidated (NQ); Oxidation (M)
4707 908.4626 4537.2766 4537.2747 0.43 1 3 0.85 +1Score > 31 indicates identity
Score > 14 indicates homology
ALQPLNIVGMDLVEVSPAYDQSDITALAGATIALDMLYLQAAK + 3 Deamidated (NQ); 2 Oxidation (M)
3100 640.0117 1917.0133 1917.0261 -6.68 1 3 0.97 +1Score > 38 indicates identity
Score > 15 indicates homology
GNREPFLQALGLLWFR + Deamidated (NQ)
3756 585.0398 2336.1301 2336.1406 -4.49 1 3 0.8 +1Score > 39 indicates identity
Score > 14 indicates homology
VFGMRHFDVQLIGGMVLNDR + Deamidated (NQ); 2 Oxidation (M)
4733 885.4474 5306.6407 5306.7258 -16.0 0 3 1 +1Score > 29 indicates identity
Score > 15 indicates homology
FGFLLMVLAVAGLMFSHSLALFSTAMFILGVVSGITMSIGTFLITQMYE + 2 Oxidation (M)
4146 927.4832 2779.4278 2779.3916 13.0 1 3 0.61 +1Score > 36 indicates identity
Score > 13 indicates homology
VDAGTQDQLQIDSVFKGSNLFVATPK + 2 Deamidated (NQ)
4513 662.3704 3306.8156 3306.8056 3.02 1 3 0.6 +1Score > 30 indicates identity
Score > 13 indicates homology
YPPSIGSILLIIPIFLTFFGNSNAQLWKR + 2 Deamidated (NQ)
3946 840.7792 2519.3158 2519.2916 9.58 1 3 0.87 +1Score > 36 indicates identity
Score > 14 indicates homology
AVWQVYQAVELPLIGMGGIMTAR + Deamidated (NQ); Oxidation (M)
2843 588.0015 1760.9827 1760.9672 8.76 1 2 1 +1Score > 35 indicates identity
Score > 15 indicates homology
VLPSQKGPSGIQPKPVE + Deamidated (NQ)
2832 877.4731 1752.9316 1752.9006 17.7 1 2 0.81 +1Score > 38 indicates identity
Score > 14 indicates homology
RPVSSDDVETAISHIK
3160 495.5148 1978.0301 1978.0047 12.8 0 2 0.86 +1Score > 39 indicates identity
Score > 14 indicates homology
TVQIVPHLTNAIQDWIE + 2 Deamidated (NQ)
2779 860.4294 1718.8442 1718.8183 15.1 1 2 0.91 +1Score > 41 indicates identity
Score > 15 indicates homology
QSAQPNASQATTRTTR + 2 Deamidated (NQ)
4358 1021.5326 3061.5760 3061.6012 -8.24 1 2 0.59 +1Score > 36 indicates identity
Score > 13 indicates homology
SRSGLGLGIGISFFATLVSQLLVYNGVYE + 2 Deamidated (NQ)
4118 917.4539 2749.3399 2749.3388 0.40 1 2 0.87 +1Score > 38 indicates identity
Score > 14 indicates homology
YVQALFSNHYFRLYTNTDVIGVE + Deamidated (NQ)
4357 766.3998 3061.5701 3061.5136 18.5 1 2 1 +1Score > 36 indicates identity
Score > 15 indicates homology
TNMFISMALVEAMPIIVIAMSFVLINE + Deamidated (NQ); 4 Oxidation (M)
3249 1012.5368 2023.0590 2023.0810 -10.9 1 2 0.91 +1Score > 38 indicates identity
Score > 15 indicates homology
HNLRVTTLGAGSNLLVSDR + Deamidated (NQ)
3487 722.4155 2164.2247 2164.1852 18.3 1 2 1 +1Score > 31 indicates identity
Score > 15 indicates homology
DTSIVLDTQLSHRLLPSLR + Deamidated (NQ)
3781 787.4285 2359.2637 2359.2682 -1.92 1 2 0.68 +1Score > 36 indicates identity
Score > 13 indicates homology
TVGLLVVAGDRGLCGAYNANVIK
4402 628.3188 3136.5576 3136.5505 2.26 1 2 0.83 +1Score > 36 indicates identity
Score > 14 indicates homology
SFIHAPGRDLDSNFLLGIYNLFLGQAQE + 2 Deamidated (NQ)
2880 593.6563 1777.9471 1777.9436 1.93 0 2 0.59 +1Score > 38 indicates identity
Score > 13 indicates homology
AMVEPYLLQIGFLQR + Deamidated (NQ)
3431 1067.5970 2133.1794 2133.1654 6.58 1 2 0.66 +1Score > 34 indicates identity
Score > 13 indicates homology
AAASGITQAVILAAGLDARAHR + Deamidated (NQ)
3747 775.3987 2323.1743 2323.2206 -19.9 1 2 0.99 +1Score > 38 indicates identity
Score > 15 indicates homology
SVVRVPTSSMSLLDGTYNLLR + Oxidation (M)
4416 792.4001 3165.5713 3165.5362 11.1 1 2 0.6 +1Score > 36 indicates identity
Score > 13 indicates homology
FKGNNSGQPSTVVDNTLMVLITMYYALR + 2 Deamidated (NQ); 2 Oxidation (M)
4173 936.8436 2807.5090 2807.4579 18.2 1 2 1 +1Score > 34 indicates identity
Score > 15 indicates homology
GHALYVEPHGHGPSLSVQGLSRASPVR
3567 735.3850 2203.1332 2203.1484 -6.92 1 2 0.89 +1Score > 38 indicates identity
Score > 14 indicates homology
AALQLGYVQSNVTARIQQLE + 2 Deamidated (NQ)
2316 755.3896 1508.7646 1508.7722 -5.03 1 2 1 +1Score > 41 indicates identity
Score > 15 indicates homology
IQNGTATIEVTFSK + Deamidated (NQ)
3186 663.3519 1987.0339 1987.0522 -9.24 0 2 1 +1Score > 39 indicates identity
Score > 15 indicates homology
TVQITIMSLIAMTVYFR + Deamidated (NQ)
4163 931.1387 2790.3943 2790.4312 -13.2 0 2 0.75 +1Score > 37 indicates identity
Score > 14 indicates homology
FLVGFIGVFFVAVLALMMGHQSTMK + 3 Oxidation (M)
4277 737.8790 2947.4869 2947.4420 15.2 1 2 0.64 +1Score > 37 indicates identity
Score > 13 indicates homology
RADGYHQVVMVMQTIALADTVTVAVNE + Deamidated (NQ); Oxidation (M)
4614 927.7238 3706.8661 3706.8741 -2.15 1 2 1 +1Score > 34 indicates identity
Score > 15 indicates homology
MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPR + 2 Oxidation (M)
3898 825.4126 2473.2160 2473.2384 -9.05 1 2 0.81 +1Score > 38 indicates identity
Score > 14 indicates homology
LSGALERPGHPLVGQDAGILAGCGE
3977 854.4571 2560.3495 2560.3723 -8.91 1 2 0.82 +1Score > 36 indicates identity
Score > 14 indicates homology
ILYATQYDTKPPKIALVMNRPE
4109 913.5095 2737.5067 2737.5088 -0.77 1 2 1 +1Score > 32 indicates identity
Score > 15 indicates homology
YQATSLPLVPPILVAMVNGADQIKAK + Deamidated (NQ)
4345 760.4365 3037.7169 3037.7732 -18.5 1 2 0.6 +1Score > 27 indicates identity
Score > 13 indicates homology
LAFALAGALIFALALFGNSLVIYVVTRSK
221 502.2586 501.2513 501.2547 -6.74 0 2 0.94 +1Score > 39 indicates identity
Score > 15 indicates homology
AGNLQ
3664 758.3867 2272.1383 2272.1647 -11.6 1 2 0.88 +1Score > 39 indicates identity
Score > 14 indicates homology
AGSLWRQLFQAGARPCGLGAR + Deamidated (NQ)
3857 807.7286 2420.1640 2420.1756 -4.79 1 2 0.76 +1Score > 39 indicates identity
Score > 14 indicates homology
NVLIAMSDMPKFYADILQYR + Deamidated (NQ); 2 Oxidation (M)
3984 642.3397 2565.3297 2565.3115 7.09 1 2 0.78 +1Score > 37 indicates identity
Score > 14 indicates homology
FIVRSQLDAYDPNLPGTGVFDLK + Deamidated (NQ)
4145 926.5179 2776.5319 2776.5237 2.93 1 2 1 +1Score > 31 indicates identity
Score > 15 indicates homology
AKNLILWLVIAVVLMSVFQSFGPSE + Oxidation (M)
4200 710.3536 2837.3853 2837.3946 -3.27 1 2 1 +1Score > 37 indicates identity
Score > 15 indicates homology
SLDEILADIRPINSLHFSFMLDFE + Oxidation (M)
4641 985.9663 3939.8361 3939.7648 18.1 1 2 1 +1Score > 33 indicates identity
Score > 15 indicates homology
MFPNGYTDDNIPDVSTWDAFQIWMRAAALPTFSK + 3 Deamidated (NQ); 2 Oxidation (M)
3848 806.7512 2417.2318 2417.1896 17.4 1 2 0.7 +1Score > 38 indicates identity
Score > 13 indicates homology
SFLPQLSDAMAAQKVTLHASQR + 3 Deamidated (NQ); Oxidation (M)
3810 795.7192 2384.1358 2384.1709 -14.7 1 2 1 +1Score > 39 indicates identity
Score > 15 indicates homology
IVPFTYQLQMGDQIEIITQK + 4 Deamidated (NQ); Oxidation (M)
4178 702.8956 2807.5533 2807.5605 -2.58 1 2 0.64 +1Score > 31 indicates identity
Score > 13 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
4018 873.7424 2618.2054 2618.2356 -11.6 1 2 0.61 +1Score > 37 indicates identity
Score > 13 indicates homology
IIVTNQTTMSQWDVQALMEHVR + 3 Deamidated (NQ); Oxidation (M)
3925 835.0889 2502.2449 2502.2446 0.10 1 2 0.89 +1Score > 38 indicates identity
Score > 14 indicates homology
TAQMGWSLARLIAQMGWGAPAMR
4393 780.9184 3119.6445 3119.6787 -11.0 1 2 0.95 +1Score > 34 indicates identity
Score > 15 indicates homology
SVILINFCLSIISSNALILIGQTQTRNK + 4 Deamidated (NQ)
746 806.4320 805.4247 805.4116 16.3 1 2 0.96 +1Score > 41 indicates identity
Score > 15 indicates homology
DGMISKR
3862 808.4634 2422.3684 2422.4096 -17.0 0 2 1 +1Score > 30 indicates identity
Score > 15 indicates homology
LAILLFALCIVFTMLTTLVVR + Oxidation (M)
4056 672.0703 2684.2521 2684.2606 -3.16 1 2 0.83 +1Score > 37 indicates identity
Score > 14 indicates homology
KFISITVDGVTYNATPNTHSNFQE + 2 Deamidated (NQ)
4426 795.9388 3179.7261 3179.7186 2.37 1 2 1 +1Score > 32 indicates identity
Score > 15 indicates homology
MVIQPSLLLLLLLTLQDVDSCQGPELVR + Deamidated (NQ); Oxidation (M)
4490 817.1578 3264.6021 3264.6428 -12.5 1 2 0.75 +1Score > 36 indicates identity
Score > 14 indicates homology
AFIAGRALAGAANPAQVAGDFHAQIDAIWGGAR + 2 Deamidated (NQ)
4201 711.3450 2841.3509 2841.3347 5.69 1 2 0.9 +1Score > 37 indicates identity
Score > 14 indicates homology
QPGTLEVVSGCTMDMLQAVYGLDGIR + 2 Oxidation (M)
3473 718.3889 2152.1449 2152.1813 -16.9 1 2 0.96 +1Score > 37 indicates identity
Score > 15 indicates homology
TQQIRPLNILILNLMPEK + 3 Deamidated (NQ); Oxidation (M)
3969 851.1239 2550.3499 2550.3006 19.3 0 2 0.79 +1Score > 35 indicates identity
Score > 14 indicates homology
TGNQWKPLTGGIFPLDLLHQIAE + 3 Deamidated (NQ)
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