MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4610 921.7322 3682.8997 3682.9088 -2.46 1 3 0.53 +1Score > 33 indicates identity
Score > 13 indicates homology
MAPPSITKTATQQDVISTVDIGNSPLLSISLDQSR
1569 1156.6233 1155.6160 1155.6248 -7.58 1 3 0.86 +1Score > 40 indicates identity
Score > 15 indicates homology
QPSVVQRTLE
4294 990.1890 2967.5452 2967.4914 18.1 0 3 0.6 +1Score > 35 indicates identity
Score > 14 indicates homology
YMHLNIPFYGVNLGSLGFLMNPLDIK + 2 Deamidated (NQ)
3375 702.7071 2105.0995 2105.1004 -0.45 1 3 0.48 +1Score > 38 indicates identity
Score > 13 indicates homology
EGQAILGALPQGADVIALDPR + 2 Deamidated (NQ)
2673 834.9339 1667.8532 1667.8454 4.73 1 3 0.79 +1Score > 40 indicates identity
Score > 15 indicates homology
RTLTYLSQVYCHK
3428 712.0550 2133.1432 2133.1503 -3.36 1 3 1 +1Score > 37 indicates identity
Score > 16 indicates homology
TALVGAVVKANGYGLGAMQIAK + 2 Deamidated (NQ)
4182 703.1408 2808.5341 2808.4810 18.9 1 3 0.71 +1Score > 32 indicates identity
Score > 14 indicates homology
IPAQVRPLYQLWRDGQALQLQLAE + 3 Deamidated (NQ)
2259 740.3466 1478.6786 1478.6745 2.78 0 3 0.73 +1Score > 40 indicates identity
Score > 14 indicates homology
SDVPLDICTATCK
2908 601.0182 1800.0328 1800.0292 2.01 1 3 0.76 +1Score > 32 indicates identity
Score > 15 indicates homology
VVSVAMVTNNVVVKGLR + Oxidation (M)
3422 710.3893 2128.1461 2128.1099 17.0 1 3 0.95 +1Score > 36 indicates identity
Score > 16 indicates homology
MAQIQFSRGVAEPVIPDVR + Oxidation (M)
2670 834.4654 1666.9162 1666.8964 11.9 1 3 0.81 +1Score > 37 indicates identity
Score > 15 indicates homology
LKPAMDKDGKPLLPE + Oxidation (M)
3390 529.5410 2114.1349 2114.1008 16.1 0 3 0.84 +1Score > 37 indicates identity
Score > 15 indicates homology
SLDLVANSADVANIWVTGLR + Deamidated (NQ)
2160 475.9123 1424.7151 1424.7003 10.3 0 3 1 +1Score > 40 indicates identity
Score > 16 indicates homology
TGTLSMTVQAMLR + Deamidated (NQ); Oxidation (M)
3680 764.3776 2290.1110 2290.1258 -6.49 0 3 0.69 +1Score > 39 indicates identity
Score > 14 indicates homology
ISVCNNSSLPMLLNMILLQE + 2 Deamidated (NQ)
2269 498.2966 1491.8680 1491.8483 13.2 1 3 0.49 +1Score > 32 indicates identity
Score > 13 indicates homology
LHMKTLLPVSKPE
3983 514.0710 2565.3186 2565.3045 5.51 0 3 0.9 +1Score > 37 indicates identity
Score > 15 indicates homology
TPTYMNIMLAFTISLLGMLTYR + Oxidation (M)
4012 650.3509 2597.3745 2597.3676 2.66 1 3 0.57 +1Score > 35 indicates identity
Score > 13 indicates homology
SPVMIGGGAIFGPKPHDYVVGINKK + Deamidated (NQ); Oxidation (M)
4062 897.4612 2689.3618 2689.3303 11.7 1 3 0.83 +1Score > 37 indicates identity
Score > 15 indicates homology
LVDMGEAVGIIAAQSIGEPGTQLTMR + Deamidated (NQ); 2 Oxidation (M)
4404 1046.5635 3136.6687 3136.6656 0.98 1 3 0.74 +1Score > 33 indicates identity
Score > 15 indicates homology
EPQLLVLDEPVQGVDVVGQTELYNLITR
4432 1061.5298 3181.5676 3181.5432 7.65 1 3 0.73 +1Score > 36 indicates identity
Score > 14 indicates homology
HLGMSLPMMQPVLQKLNQLYQVHICGE + 2 Deamidated (NQ); Oxidation (M)
4411 1055.5110 3163.5112 3163.4703 12.9 1 3 0.82 +1Score > 36 indicates identity
Score > 15 indicates homology
QSEFLVCSGGGSYDSGAMALVVNHHGDIIK + Oxidation (M)
3870 610.0842 2436.3077 2436.3012 2.66 1 3 0.75 +1Score > 35 indicates identity
Score > 14 indicates homology
SLKGSNKPFLYTNGTLSLRPNK + 2 Deamidated (NQ)
4434 1064.5581 3190.6525 3190.6218 9.61 1 3 0.66 +1Score > 34 indicates identity
Score > 14 indicates homology
LSHLNNAQKQDLNTQVTNAPNISTVNQVK + 2 Deamidated (NQ)
2059 461.5950 1381.7632 1381.7565 4.83 1 3 0.8 +1Score > 37 indicates identity
Score > 15 indicates homology
IPPDLKTLQSNR + Deamidated (NQ)
3837 805.0841 2412.2305 2412.2094 8.74 1 3 0.64 +1Score > 38 indicates identity
Score > 14 indicates homology
GLNLGDSLVEPESVPVAQQLMAK + 2 Deamidated (NQ); Oxidation (M)
3641 563.7846 2251.1093 2251.0799 13.1 1 3 0.88 +1Score > 39 indicates identity
Score > 15 indicates homology
MTKIVMLDYIPMLNNGGHR + Deamidated (NQ); 3 Oxidation (M)
3669 759.3491 2275.0255 2275.0024 10.1 1 3 0.73 +1Score > 37 indicates identity
Score > 14 indicates homology
LILSNQYYLMSQMDPENAE + Deamidated (NQ); Oxidation (M)
2945 604.9980 1811.9722 1811.9815 -5.15 0 3 0.97 +1Score > 38 indicates identity
Score > 16 indicates homology
LLQSSGLLPVAQQVCAK + Deamidated (NQ)
3888 616.3384 2461.3245 2461.3073 6.99 1 3 0.81 +1Score > 35 indicates identity
Score > 15 indicates homology
SLTKIMPIHGTAAVSLPMTIHVE + Oxidation (M)
4080 901.8501 2702.5285 2702.5040 9.04 0 3 1 +1Score > 29 indicates identity
Score > 16 indicates homology
NAIATVLLGLAMLLPVGAVQAQDGPLR + 2 Deamidated (NQ)
4373 772.4276 3085.6813 3085.6416 12.9 1 3 0.84 +1Score > 31 indicates identity
Score > 15 indicates homology
VQKFFLTQNTILSLLNWSFPIFDLAK + 3 Deamidated (NQ)
3462 537.7672 2147.0397 2147.0416 -0.88 1 3 0.89 +1Score > 40 indicates identity
Score > 15 indicates homology
VLGALGNAVKNVDNLSQAMSE + 2 Deamidated (NQ); Oxidation (M)
3973 852.4568 2554.3486 2554.3551 -2.54 1 3 0.55 +1Score > 36 indicates identity
Score > 13 indicates homology
HIDQQIHGGLLGGAAGVGMLGRLQR + Deamidated (NQ)
4128 920.1796 2757.5170 2757.5132 1.37 1 3 1 +1Score > 32 indicates identity
Score > 16 indicates homology
DMLVILAVLSMAIGNIAAIAQSNLKR + Deamidated (NQ); 2 Oxidation (M)
3947 631.5931 2522.3433 2522.3931 -19.7 0 3 0.58 +1Score > 35 indicates identity
Score > 13 indicates homology
GILTTAHSVIQTPVFMPVGTVGAVK
2783 574.3147 1719.9223 1719.9268 -2.62 1 3 0.74 +1Score > 38 indicates identity
Score > 14 indicates homology
QVAGGRLLNTLYTTGR + Deamidated (NQ)
3352 697.7365 2090.1877 2090.1623 12.1 1 3 1 +1Score > 31 indicates identity
Score > 16 indicates homology
YIVNTVLDLGGQTTKILDK
4527 1111.9320 3332.7742 3332.7149 17.8 1 3 0.8 +1Score > 32 indicates identity
Score > 15 indicates homology
SLPWQGMAQSALITVPPLGCLVIGPATGLAEAN + Deamidated (NQ)
296 587.3516 586.3443 586.3551 -18.4 1 3 0.99 +1Score > 45 indicates identity
Score > 16 indicates homology
AGVGKR
2710 562.9667 1685.8783 1685.8696 5.13 0 3 0.96 +1Score > 40 indicates identity
Score > 15 indicates homology
SSSIHSIGSLASNALSR
3243 1012.5291 2023.0436 2023.0408 1.39 0 3 0.87 +1Score > 39 indicates identity
Score > 15 indicates homology
VTALADGVLSTGQMVTYAAR
3514 726.7471 2177.2195 2177.2017 8.15 1 3 1 +1Score > 32 indicates identity
Score > 16 indicates homology
TVIPSLKIFAGMLDTLTVNK + Deamidated (NQ); Oxidation (M)
3921 833.4047 2497.1923 2497.1907 0.62 1 3 1 +1Score > 38 indicates identity
Score > 16 indicates homology
NLPSLQPLELHGDRPGLCDVHE + 2 Deamidated (NQ)
4262 975.1920 2922.5542 2922.4975 19.4 1 3 0.87 +1Score > 34 indicates identity
Score > 15 indicates homology
IVQYGPSASAVILGQGTSTDIRFDNLAK + 2 Deamidated (NQ)
3907 828.1116 2481.3130 2481.3075 2.21 1 3 0.96 +1Score > 36 indicates identity
Score > 15 indicates homology
QARSPPTSASVSTVIDGPTQILVR + 2 Deamidated (NQ)
3010 620.6550 1858.9432 1858.9173 13.9 1 3 0.66 +1Score > 40 indicates identity
Score > 14 indicates homology
INRDGDVSDIAVPFANR + Deamidated (NQ)
3757 780.3775 2338.1107 2338.1297 -8.13 1 3 0.63 +1Score > 39 indicates identity
Score > 14 indicates homology
ALGMQDVVAKSLGSSNPYNMVR + 2 Deamidated (NQ)
4591 894.4977 3573.9617 3573.8945 18.8 1 3 1 +1Score > 29 indicates identity
Score > 16 indicates homology
NNNIMNLIAAIVLSLSIIFGWQYFVVKPEHK + 3 Deamidated (NQ)
2825 581.6689 1741.9849 1741.9574 15.8 1 3 0.98 +1Score > 34 indicates identity
Score > 16 indicates homology
ALSVQVAVTAQRIQQK + 3 Deamidated (NQ)
3833 602.0776 2404.2813 2404.2651 6.72 1 3 0.59 +1Score > 36 indicates identity
Score > 13 indicates homology
VAILAVRADPAFGNLWHQLNAE
3942 838.4315 2512.2727 2512.2420 12.2 1 3 0.62 +1Score > 38 indicates identity
Score > 14 indicates homology
FEIVLYGATGFSGMLTGQHLAQR + Deamidated (NQ); Oxidation (M)
4076 676.6064 2702.3965 2702.3731 8.67 1 3 1 +1Score > 36 indicates identity
Score > 16 indicates homology
AVQFIDLLQKVYNDFGFNDILVK + 4 Deamidated (NQ)
4194 942.4597 2824.3573 2824.3953 -13.5 1 3 0.77 +1Score > 37 indicates identity
Score > 14 indicates homology
IQTRGVTSLTTIWSISPTPNCSIYE + Deamidated (NQ)
3954 843.0930 2526.2572 2526.2887 -12.5 1 3 0.81 +1Score > 38 indicates identity
Score > 15 indicates homology
LAELGPVALVDCLSDIADGSAIAQK + Deamidated (NQ)
4049 890.4987 2668.4743 2668.4211 19.9 1 3 0.77 +1Score > 31 indicates identity
Score > 14 indicates homology
SPFLLSTLDADIGDAVIEIDGIPIGK
2812 578.6567 1732.9483 1732.9611 -7.38 1 3 0.95 +1Score > 37 indicates identity
Score > 15 indicates homology
LYGSTNALVLLIAQEK + Deamidated (NQ)
3945 630.5921 2518.3393 2518.3339 2.15 1 3 0.89 +1Score > 35 indicates identity
Score > 15 indicates homology
MLLPGHPRPPPAPQSAQNQGLRR + Deamidated (NQ)
4102 683.5801 2730.2913 2730.3456 -19.9 1 3 0.57 +1Score > 38 indicates identity
Score > 13 indicates homology
MRGANVTDIVILVVAADDGVMPQTVE + 2 Deamidated (NQ); Oxidation (M)
4510 827.7092 3306.8077 3306.7890 5.65 0 3 0.96 +1Score > 30 indicates identity
Score > 15 indicates homology
ISLGQLITYNALLVFFTEPLQNIINLQVK + 5 Deamidated (NQ)
1257 1008.5189 1007.5116 1007.4923 19.1 1 3 0.76 +1Score > 42 indicates identity
Score > 14 indicates homology
LGFKSANAAE + Deamidated (NQ)
4025 878.4568 2632.3486 2632.3306 6.83 1 3 0.57 +1Score > 37 indicates identity
Score > 13 indicates homology
VQSYLTSTSSTFKLADTVILICGE
4063 897.7803 2690.3191 2690.3050 5.22 1 3 0.92 +1Score > 38 indicates identity
Score > 15 indicates homology
MIGFNPEQINFVPVASLHGDNVFK + 2 Deamidated (NQ); Oxidation (M)
3252 1014.5035 2026.9924 2026.9921 0.16 1 3 0.88 +1Score > 40 indicates identity
Score > 15 indicates homology
TMSDLLGLLKNWDVDYK + Deamidated (NQ); Oxidation (M)
340 618.3068 617.2995 617.3020 -4.06 0 3 0.85 +1Score > 45 indicates identity
Score > 15 indicates homology
VNDAAK + Deamidated (NQ)
3360 699.3757 2095.1053 2095.0805 11.8 1 3 0.55 +1Score > 37 indicates identity
Score > 13 indicates homology
YNMANSLLVLAAKLGMDVR + Deamidated (NQ); Oxidation (M)
3406 530.5607 2118.2137 2118.2453 -14.9 1 3 1 +1Score > 30 indicates identity
Score > 15 indicates homology
GISAVVELAATVLVTFFLLR
3409 707.3696 2119.0870 2119.0521 16.5 1 3 0.84 +1Score > 39 indicates identity
Score > 15 indicates homology
AYVDVCNGIVPSWADGIKR
3760 780.7206 2339.1400 2339.1063 14.4 0 3 0.78 +1Score > 39 indicates identity
Score > 14 indicates homology
NTNTYAIIQMGGQQLQVQTGR + 3 Deamidated (NQ); Oxidation (M)
4264 732.3988 2925.5661 2925.5239 14.4 0 3 0.75 +1Score > 33 indicates identity
Score > 14 indicates homology
AMQVAGAVTPVPGGVGQMTVACLLVNTLR + Oxidation (M)
2886 596.3292 1785.9658 1785.9924 -14.9 0 3 0.98 +1Score > 38 indicates identity
Score > 15 indicates homology
IGVLIHPQSVIHSMVR + Deamidated (NQ)
4241 724.1239 2892.4665 2892.4633 1.11 1 3 0.57 +1Score > 36 indicates identity
Score > 13 indicates homology
RTILQMSVWQDWLIGLAYVFHTSE
4284 740.1357 2956.5137 2956.4950 6.31 1 3 1 +1Score > 36 indicates identity
Score > 15 indicates homology
NIRPNIILVLTDDQDVELGSLQVMNK + 4 Deamidated (NQ); Oxidation (M)
4359 1021.8846 3062.6320 3062.5780 17.6 0 3 1 +1Score > 33 indicates identity
Score > 15 indicates homology
LIPSGILHNNVTCLIGNGVVLSPSALMQE + Deamidated (NQ); Oxidation (M)
2158 712.8697 1423.7248 1423.7082 11.7 0 3 0.86 +1Score > 40 indicates identity
Score > 15 indicates homology
NPQILQQLQQPL + 5 Deamidated (NQ)
4180 702.8959 2807.5545 2807.5605 -2.15 1 3 0.68 +1Score > 31 indicates identity
Score > 14 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
4263 732.3915 2925.5369 2925.5239 4.45 0 3 0.85 +1Score > 35 indicates identity
Score > 15 indicates homology
AMQVAGAVTPVPGGVGQMTVACLLVNTLR + Oxidation (M)
692 783.3997 782.3924 782.3851 9.42 1 3 0.81 +1Score > 39 indicates identity
Score > 15 indicates homology
EDFLIF
3101 640.3187 1917.9343 1917.9683 -17.8 0 3 0.86 +1Score > 40 indicates identity
Score > 15 indicates homology
YVITNGNQLAIQNDLLE + Deamidated (NQ)
3396 1058.5889 2115.1632 2115.1436 9.27 1 3 0.85 +1Score > 35 indicates identity
Score > 15 indicates homology
QAGLGRAGDSAVLVLPPSPGPR + Deamidated (NQ)
3583 738.0322 2211.0748 2211.1034 -13.0 1 3 0.66 +1Score > 39 indicates identity
Score > 14 indicates homology
WNLAVIQLFSAEGMDTFIR + Deamidated (NQ)
3617 747.0335 2238.0787 2238.1024 -10.6 1 3 0.79 +1Score > 39 indicates identity
Score > 14 indicates homology
TSMSCNLSLYEHLVNLLTK + Oxidation (M)
4374 1029.9052 3086.6938 3086.6607 10.7 1 3 0.74 +1Score > 31 indicates identity
Score > 14 indicates homology
SNTLAGQEVLVNLLLALLLDVMTVSGMQK + Deamidated (NQ); Oxidation (M)
2763 571.3060 1710.8962 1710.9039 -4.54 0 3 0.58 +1Score > 39 indicates identity
Score > 13 indicates homology
IAQIQSTLPNATPNLK + 3 Deamidated (NQ)
4463 803.6701 3210.6513 3210.6812 -9.32 1 3 0.76 +1Score > 35 indicates identity
Score > 14 indicates homology
NGYYVVTPQAWKTTILANSSIVISIQGTGK + 2 Deamidated (NQ)
3194 664.3530 1990.0372 1990.0007 18.3 1 3 0.99 +1Score > 39 indicates identity
Score > 15 indicates homology
GIDDIIAVDDLTQGDKFR
4661 1016.2915 4061.1369 4061.0667 17.3 1 3 1 +1Score > 30 indicates identity
Score > 15 indicates homology
VPNLLLNGASGIAVGMATNIPPHNLGELVDALSALIHNPE + 4 Deamidated (NQ); Oxidation (M)
3853 806.7609 2417.2609 2417.2624 -0.64 0 3 0.59 +1Score > 37 indicates identity
Score > 13 indicates homology
MIYQSTLNKPLTISGIGLHTGR + 2 Deamidated (NQ); Oxidation (M)
3274 1020.4940 2038.9734 2039.0106 -18.2 0 3 1 +1Score > 39 indicates identity
Score > 15 indicates homology
GPVNGDTMTGQNKPVKPNGK + Deamidated (NQ)
2061 692.3896 1382.7646 1382.7881 -17.0 1 3 0.89 +1Score > 38 indicates identity
Score > 15 indicates homology
DALDKAQAILAVR
4440 639.9221 3194.5741 3194.5753 -0.38 0 3 0.61 +1Score > 36 indicates identity
Score > 13 indicates homology
VLQRPVLSSWQALLDYCHTAMAHRPTE + Oxidation (M)
4581 1185.6145 3553.8217 3553.7657 15.7 1 3 1 +1Score > 34 indicates identity
Score > 15 indicates homology
VDSHIMKVVQQIVHQAGAGHQIIVQNVTMDQE + 2 Deamidated (NQ)
2938 904.9915 1807.9684 1807.9866 -10.0 1 3 0.84 +1Score > 37 indicates identity
Score > 15 indicates homology
VVSAYQMGVSALKQALK + Oxidation (M)
3417 709.7292 2126.1658 2126.1591 3.12 1 3 0.79 +1Score > 35 indicates identity
Score > 14 indicates homology
MLTAEMVKPGAIVIDVGINR
3960 846.7980 2537.3722 2537.3741 -0.75 1 3 0.71 +1Score > 34 indicates identity
Score > 14 indicates homology
EINQWTGQLLAIPLTTATPLTVR + 2 Deamidated (NQ)
3751 777.4199 2329.2379 2329.1914 20.0 0 3 0.56 +1Score > 36 indicates identity
Score > 13 indicates homology
HLLNIVPQLNDSIDDPQIAGR + 2 Deamidated (NQ)
2688 557.5912 1669.7518 1669.7583 -3.94 1 3 0.77 +1Score > 39 indicates identity
Score > 14 indicates homology
AAVAQQYPAYSGGRTE + 2 Deamidated (NQ)
3807 794.1094 2379.3064 2379.2692 15.6 1 3 0.83 +1Score > 33 indicates identity
Score > 15 indicates homology
ALNQTGLKCLVISGGVSANHALR + Deamidated (NQ)
4022 876.7502 2627.2288 2627.2652 -13.9 1 3 0.69 +1Score > 37 indicates identity
Score > 14 indicates homology
QPIQITMPFTDIGTFETMWQVK + Deamidated (NQ); Oxidation (M)
4105 911.7939 2732.3599 2732.3327 9.95 1 3 0.7 +1Score > 37 indicates identity
Score > 14 indicates homology
SISINVPPGVMEGNYLTLGDAGNAGLR + 2 Deamidated (NQ); Oxidation (M)
201 474.2921 473.2848 473.2849 -0.25 0 3 0.63 +1Score > 43 indicates identity
Score > 13 indicates homology
ITGIA
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