MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4216 956.4818 2866.4236 2866.4106 4.54 1 4 1 +1Score > 37 indicates identity
Score > 17 indicates homology
FSILSKPGGQPCLILSGNGNQAKCYR + 2 Deamidated (NQ)
3692 574.7958 2295.1541 2295.1464 3.37 1 4 0.98 +1Score > 38 indicates identity
Score > 16 indicates homology
AIAAHAARGGAVLGVCGGLQMLGE + Deamidated (NQ); Oxidation (M)
3771 785.4089 2353.2049 2353.2278 -9.72 1 4 0.42 +1Score > 38 indicates identity
Score > 13 indicates homology
LWQQATQLGQQALLAAGTPTKE + Deamidated (NQ)
4390 1040.5425 3118.6057 3118.6299 -7.76 1 4 1 +1Score > 35 indicates identity
Score > 17 indicates homology
LGIPVVAILDSNSDPRGVTFPIPGNDDAIR + Deamidated (NQ)
2808 867.0349 1732.0552 1732.0359 11.2 1 4 0.75 +1Score > 25 indicates identity
Score > 15 indicates homology
VIKTQNLAALLHAIAR + Deamidated (NQ)
3670 759.7230 2276.1472 2276.1484 -0.54 1 4 0.93 +1Score > 39 indicates identity
Score > 16 indicates homology
NPQSVNIHCLKAHSPLASFR + Deamidated (NQ)
4176 702.8947 2807.5497 2807.5605 -3.86 1 4 0.54 +1Score > 31 indicates identity
Score > 14 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
2614 547.2952 1638.8638 1638.8949 -19.0 0 4 0.98 +1Score > 39 indicates identity
Score > 16 indicates homology
LQMQHLILIVVCR + Deamidated (NQ); Oxidation (M)
3957 633.8309 2531.2945 2531.3206 -10.3 1 4 0.47 +1Score > 38 indicates identity
Score > 13 indicates homology
MTSHVLIATLAIPLPSAHPEFDR + Oxidation (M)
961 450.2891 898.5636 898.5487 16.6 0 4 0.71 +1Score > 37 indicates identity
Score > 15 indicates homology
LISNVKPK + Deamidated (NQ)
1230 997.5155 996.5082 996.5175 -9.30 0 4 0.97 +1Score > 40 indicates identity
Score > 16 indicates homology
QTPVMIHR + Oxidation (M)
3387 705.0613 2112.1621 2112.1467 7.29 0 4 0.63 +1Score > 34 indicates identity
Score > 14 indicates homology
INGGIVLTGGGALFPGISNLIE + Deamidated (NQ)
2114 468.9233 1403.7481 1403.7330 10.7 0 4 0.74 +1Score > 39 indicates identity
Score > 15 indicates homology
IVDSNNQMLLLK + Deamidated (NQ); Oxidation (M)
3659 756.3916 2266.1530 2266.1118 18.2 1 4 0.84 +1Score > 38 indicates identity
Score > 16 indicates homology
AGATTVNIPDTVGYSTPLEFGR + Deamidated (NQ)
3902 827.4254 2479.2544 2479.2853 -12.5 1 4 0.87 +1Score > 38 indicates identity
Score > 16 indicates homology
GLSCAQARIQIVADLQAHGLLSR + 3 Deamidated (NQ)
4521 1107.2097 3318.6073 3318.5714 10.8 1 4 0.7 +1Score > 36 indicates identity
Score > 15 indicates homology
ASSMIFLDQPVGAGFSYSRTQLLNKPSDSGE + Deamidated (NQ); Oxidation (M)
2598 408.7468 1630.9581 1630.9406 10.7 0 4 0.89 +1Score > 31 indicates identity
Score > 16 indicates homology
LIQLGVFTGLSNLTR
2080 463.9355 1388.7847 1388.7888 -2.97 1 4 0.88 +1Score > 36 indicates identity
Score > 16 indicates homology
AVRAATYGGALALR
3566 735.3604 2203.0594 2203.0579 0.66 0 4 0.87 +1Score > 39 indicates identity
Score > 16 indicates homology
MYYSTAVTGNIVNSVNVQSR + Deamidated (NQ)
3797 475.4774 2372.3506 2372.3427 3.34 1 4 0.99 +1Score > 30 indicates identity
Score > 16 indicates homology
RFVSIINGDNLSLSIAAASIIAK
4190 706.3448 2821.3501 2821.3527 -0.92 1 4 0.93 +1Score > 37 indicates identity
Score > 16 indicates homology
RQQAALCAQHALNMLLQDSLFTYE + Deamidated (NQ)
3430 712.0648 2133.1726 2133.1317 19.2 1 4 0.75 +1Score > 35 indicates identity
Score > 15 indicates homology
AGFISSNQLVKVLGNGALTNK + 3 Deamidated (NQ)
3036 624.3665 1870.0777 1870.0676 5.36 0 4 0.66 +1Score > 31 indicates identity
Score > 15 indicates homology
AGPGSLGPVNLPIPVVIDR
3170 991.4892 1980.9638 1980.9826 -9.48 1 4 0.78 +1Score > 40 indicates identity
Score > 15 indicates homology
TVDFRNTVIIMTSNLGAE + Deamidated (NQ)
3656 1134.0795 2266.1444 2266.1780 -14.8 1 4 0.76 +1Score > 39 indicates identity
Score > 15 indicates homology
VQLTQQFCKLFQALGLNTR + 2 Deamidated (NQ)
4169 699.8926 2795.5413 2795.5606 -6.90 0 4 1 +1Score > 31 indicates identity
Score > 16 indicates homology
SIQSGLIEPMVILLILVANATVGVVTE + Deamidated (NQ); Oxidation (M)
3460 716.3995 2146.1767 2146.1382 17.9 1 4 1 +1Score > 35 indicates identity
Score > 16 indicates homology
SRGANSVIALGTHPVLSGPALE + Deamidated (NQ)
4387 1038.2325 3111.6757 3111.6613 4.62 1 4 1 +1Score > 32 indicates identity
Score > 16 indicates homology
NLIRMGMAGLILVVLGILIFQDWHSQR + 3 Deamidated (NQ); Oxidation (M)
3530 730.0247 2187.0523 2187.0122 18.3 1 4 0.92 +1Score > 39 indicates identity
Score > 16 indicates homology
VMLGKVNQCPHQLMNGVSR + 4 Deamidated (NQ); Oxidation (M)
3158 658.3350 1971.9832 1971.9823 0.43 0 4 0.71 +1Score > 40 indicates identity
Score > 15 indicates homology
SVEPMTVSVQPSSVQVVAE
4355 766.1797 3060.6897 3060.6342 18.1 1 4 0.79 +1Score > 30 indicates identity
Score > 15 indicates homology
LQQQQQSLLSQPAVTLAQLPLIQEQQK + 3 Deamidated (NQ)
3442 1070.0186 2138.0226 2138.0316 -4.17 1 4 0.83 +1Score > 39 indicates identity
Score > 16 indicates homology
MFLQTIPELFQMLPGIDE + Deamidated (NQ); Oxidation (M)
4331 756.3460 3021.3549 3021.3563 -0.46 1 4 0.85 +1Score > 35 indicates identity
Score > 16 indicates homology
MTQQTHFIAGQWHAGQGHDIESIDPAK + 3 Deamidated (NQ); Oxidation (M)
3407 707.3671 2119.0795 2119.0580 10.2 1 4 0.93 +1Score > 39 indicates identity
Score > 16 indicates homology
LANAEVTGQSGGGLVSVVMTGR + Deamidated (NQ); Oxidation (M)
4227 959.8298 2876.4676 2876.4655 0.74 1 4 1 +1Score > 36 indicates identity
Score > 16 indicates homology
NILVIKSSVFGDNGNSSALVNAQVDALK + 4 Deamidated (NQ)
1335 519.7720 1037.5294 1037.5366 -6.91 0 4 0.47 +1Score > 40 indicates identity
Score > 13 indicates homology
ASNNLGAVHR
2829 875.4952 1748.9758 1748.9785 -1.50 1 4 1 +1Score > 35 indicates identity
Score > 16 indicates homology
SAGPVKAPSVQPIITQR + Deamidated (NQ)
2883 595.6756 1784.0050 1783.9944 5.90 1 4 0.98 +1Score > 35 indicates identity
Score > 16 indicates homology
FLLQRQSGALVPAAGVR + 2 Deamidated (NQ)
3932 837.1096 2508.3070 2508.3298 -9.10 1 4 0.99 +1Score > 37 indicates identity
Score > 16 indicates homology
VVWLEINTQPGMTPTSLVPDIAK
4230 720.6558 2878.5941 2878.6102 -5.61 1 4 0.76 +1Score > 30 indicates identity
Score > 15 indicates homology
LQSLAQVKMKPVFNLTGIVLHTNLGR + 2 Deamidated (NQ)
3665 758.4108 2272.2106 2272.1733 16.4 1 4 0.93 +1Score > 36 indicates identity
Score > 16 indicates homology
TRDMIDNLLSPDLIDGVLTR + Oxidation (M)
1938 442.5796 1324.7170 1324.7350 -13.6 1 4 0.93 +1Score > 38 indicates identity
Score > 16 indicates homology
LVEGLNSLGIGPR + Deamidated (NQ)
4068 898.4495 2692.3267 2692.2915 13.0 0 4 0.59 +1Score > 38 indicates identity
Score > 14 indicates homology
GPPGPAGPAGLMGPPGLQGPSGLPGDPGDR + Deamidated (NQ); Oxidation (M)
3071 947.5048 1892.9950 1892.9996 -2.40 1 4 0.55 +1Score > 38 indicates identity
Score > 14 indicates homology
KALQSQSPQVYFQVLR + 2 Deamidated (NQ)
3350 1045.0619 2088.1092 2088.0964 6.16 1 4 0.62 +1Score > 37 indicates identity
Score > 14 indicates homology
DPVALIQELNQVVGSHGVGR + Deamidated (NQ)
1109 948.5027 947.4954 947.4858 10.1 1 4 0.92 +1Score > 41 indicates identity
Score > 16 indicates homology
QIAGMQKR + Deamidated (NQ); Oxidation (M)
2370 511.6362 1531.8868 1531.8974 -6.91 0 4 1 +1Score > 33 indicates identity
Score > 16 indicates homology
VPASVLAQKPIPIAE
4140 925.8442 2774.5108 2774.5001 3.86 1 4 0.73 +1Score > 33 indicates identity
Score > 15 indicates homology
VAQVPTTLLAMVDAAIGGKTGVNHPLGK + Deamidated (NQ); Oxidation (M)
4250 969.4878 2905.4416 2905.4686 -9.31 1 4 0.73 +1Score > 37 indicates identity
Score > 15 indicates homology
ITPGMLMAAMRINIPTIFVSGGPMAAGR + Deamidated (NQ); 2 Oxidation (M)
4584 893.4896 3569.9293 3569.9088 5.73 1 4 1 +1Score > 30 indicates identity
Score > 16 indicates homology
RSLHNVANYLILSLAVADLMVAVLVMPLSVVSE + 2 Deamidated (NQ); 2 Oxidation (M)
2533 805.9849 1609.9552 1609.9403 9.30 1 4 0.62 +1Score > 31 indicates identity
Score > 14 indicates homology
LSPAAVLTKLNQLGGK + Deamidated (NQ)
3072 632.0192 1893.0358 1892.9996 19.1 0 4 0.94 +1Score > 36 indicates identity
Score > 16 indicates homology
TALAAFLQYTPGVNTGLR + Deamidated (NQ)
4108 913.1732 2736.4978 2736.4480 18.2 0 4 1 +1Score > 32 indicates identity
Score > 16 indicates homology
NMLNVLQSLVTHLNQAVSQIQQLK + 2 Deamidated (NQ); Oxidation (M)
4054 892.7868 2675.3386 2675.2870 19.3 0 4 0.96 +1Score > 38 indicates identity
Score > 16 indicates homology
VNGHIIDDIMHDIMVKPLPQGCR + 2 Deamidated (NQ); Oxidation (M)
4570 862.4570 3445.7989 3445.8555 -16.4 1 4 1 +1Score > 34 indicates identity
Score > 16 indicates homology
GTVQISGAKNSAVAVLPAALLADSPSVIDNLPDIK + 2 Deamidated (NQ)
3597 557.0524 2224.1805 2224.2063 -11.6 1 4 0.88 +1Score > 36 indicates identity
Score > 16 indicates homology
GTQDLAAIVRSVVSVPDQQIK + Deamidated (NQ)
2367 511.2895 1530.8467 1530.8406 3.99 1 4 0.83 +1Score > 38 indicates identity
Score > 15 indicates homology
AQANPTISFNVLKK + Deamidated (NQ)
2625 412.2276 1644.8813 1644.8909 -5.85 0 4 0.84 +1Score > 39 indicates identity
Score > 15 indicates homology
TLPDASFLPVLSVMR
3486 722.3854 2164.1344 2164.1674 -15.3 1 4 0.54 +1Score > 37 indicates identity
Score > 13 indicates homology
GNLHINLLDSTVILCPKTR + Deamidated (NQ)
683 777.4100 776.4027 776.4068 -5.31 0 4 0.49 +1Score > 42 indicates identity
Score > 13 indicates homology
AGVLAFAE
610 748.4059 747.3986 747.3875 14.9 1 4 0.67 +1Score > 41 indicates identity
Score > 14 indicates homology
NTTGAKR + Deamidated (NQ)
3988 857.1493 2568.4261 2568.4057 7.93 1 4 0.83 +1Score > 31 indicates identity
Score > 15 indicates homology
NVLAIATGIADGMGLGLNARAALITR + Deamidated (NQ); Oxidation (M)
2260 740.3913 1478.7680 1478.7729 -3.26 0 4 0.86 +1Score > 40 indicates identity
Score > 15 indicates homology
GDLPLNINIQEPR + Deamidated (NQ)
4017 873.0919 2616.2539 2616.2953 -15.8 1 4 0.93 +1Score > 38 indicates identity
Score > 16 indicates homology
NSVLSSNVAIDDGAIVEGSVIMPGVR + 2 Deamidated (NQ); Oxidation (M)
4293 990.1862 2967.5368 2967.4914 15.3 0 4 0.66 +1Score > 35 indicates identity
Score > 14 indicates homology
YMHLNIPFYGVNLGSLGFLMNPLDIK + 2 Deamidated (NQ)
3835 803.0704 2406.1894 2406.1884 0.39 1 4 0.54 +1Score > 39 indicates identity
Score > 13 indicates homology
NFPPIKNIVMMGMGEPLLNLK + 3 Deamidated (NQ); 3 Oxidation (M)
3964 849.4554 2545.3444 2545.3653 -8.21 1 4 1 +1Score > 36 indicates identity
Score > 16 indicates homology
NVVIVGGGDSALDWTLNLHPIAKR + Deamidated (NQ)
3205 667.3632 1999.0678 1999.0594 4.16 1 4 0.87 +1Score > 37 indicates identity
Score > 15 indicates homology
SSGKPLAAPKVGMVSLGCPK + Oxidation (M)
3267 1019.5262 2037.0378 2037.0088 14.2 1 4 0.94 +1Score > 39 indicates identity
Score > 16 indicates homology
VQAVFMDLGLSSLQIDER + Deamidated (NQ); Oxidation (M)
3854 605.3235 2417.2649 2417.2914 -11.0 1 4 1 +1Score > 37 indicates identity
Score > 16 indicates homology
LTEANVPPQVLATGGGVVIRPGNR + 3 Deamidated (NQ)
2347 759.9393 1517.8640 1517.8606 2.27 1 4 0.69 +1Score > 35 indicates identity
Score > 14 indicates homology
AKNVQIFLPVDFK
2511 533.6360 1597.8862 1597.8675 11.7 1 4 0.84 +1Score > 37 indicates identity
Score > 15 indicates homology
ITIPNAISSNKSVPR + 2 Deamidated (NQ)
3284 1021.5361 2041.0576 2041.0341 11.6 1 4 0.72 +1Score > 38 indicates identity
Score > 15 indicates homology
DNAFQQVASLRLSHGQLR + 2 Deamidated (NQ)
4286 986.5379 2956.5919 2956.5408 17.3 0 4 0.86 +1Score > 33 indicates identity
Score > 15 indicates homology
YAAYGLVMPSLVLNYFGQGALLIQNPK + Deamidated (NQ); Oxidation (M)
3762 587.0208 2344.0541 2344.0390 6.44 1 4 0.7 +1Score > 37 indicates identity
Score > 15 indicates homology
DVGLPDGQFGNSEVGHMNLGAGR + 2 Deamidated (NQ); Oxidation (M)
3968 851.1226 2550.3460 2550.3006 17.8 0 4 0.92 +1Score > 36 indicates identity
Score > 16 indicates homology
TGNQWKPLTGGIFPLDLLHQIAE + 3 Deamidated (NQ)
1028 924.4808 923.4735 923.4713 2.46 1 4 0.64 +1Score > 40 indicates identity
Score > 14 indicates homology
TVKHVPDE
4452 1070.2333 3207.6781 3207.6486 9.20 0 4 1 +1Score > 34 indicates identity
Score > 16 indicates homology
LDVLNSLSQVCDQLIVGGGIANTFLAAAGYK + Deamidated (NQ)
3966 850.1099 2547.3079 2547.2672 16.0 1 4 1 +1Score > 37 indicates identity
Score > 16 indicates homology
QNKPLSPMSATALLQKAAQMGSTR + 3 Deamidated (NQ); Oxidation (M)
2657 555.2982 1662.8728 1662.8689 2.33 1 4 0.86 +1Score > 40 indicates identity
Score > 15 indicates homology
LRAISSPTFSNNSLR + Deamidated (NQ)
3565 735.3571 2203.0495 2203.0579 -3.84 0 4 1 +1Score > 39 indicates identity
Score > 16 indicates homology
MYYSTAVTGNIVNSVNVQSR + Deamidated (NQ)
768 411.2490 820.4834 820.4807 3.41 1 3 0.93 +1Score > 37 indicates identity
Score > 16 indicates homology
IKQLYR + Deamidated (NQ)
2794 576.9601 1727.8585 1727.8843 -14.9 1 3 0.67 +1Score > 40 indicates identity
Score > 14 indicates homology
VQVAPGFDVADNEIVR
3069 947.4996 1892.9846 1892.9480 19.4 1 3 0.91 +1Score > 39 indicates identity
Score > 16 indicates homology
VQDIISSYAATVQVGGER + Deamidated (NQ)
4138 925.5103 2773.5091 2773.4659 15.6 1 3 0.83 +1Score > 33 indicates identity
Score > 15 indicates homology
RGNVMGIALGGLAMGVLVGPPFGSVLYE
3265 679.3704 2035.0894 2035.0738 7.64 1 3 0.6 +1Score > 37 indicates identity
Score > 14 indicates homology
FLNQLLHIVQHQKTVSE + 2 Deamidated (NQ)
3485 722.3593 2164.0561 2164.0899 -15.6 1 3 0.73 +1Score > 39 indicates identity
Score > 15 indicates homology
GVGYNIAVVKDNITITQNNK + 4 Deamidated (NQ)
4087 903.4880 2707.4422 2707.4268 5.67 1 3 0.69 +1Score > 35 indicates identity
Score > 14 indicates homology
WIGSVNSIGMAAGAFLFGLLADRIGR + Oxidation (M)
4245 967.5058 2899.4956 2899.4657 10.3 1 3 0.76 +1Score > 36 indicates identity
Score > 15 indicates homology
LYRNYLSSPFPGNFSQPSPFLQIAR + Deamidated (NQ)
4618 935.5103 3738.0121 3737.9855 7.11 1 3 1 +1Score > 30 indicates identity
Score > 16 indicates homology
TNSPLDGFATVVIANLFFGAVQLICIGILGEYIGR
399 326.6993 651.3840 651.3816 3.70 0 3 0.48 +1Score > 34 indicates identity
Score > 13 indicates homology
AIGHVR
2404 775.9172 1549.8198 1549.8464 -17.1 1 3 0.74 +1Score > 39 indicates identity
Score > 15 indicates homology
VALELDLHPSTISR
3214 1004.5901 2007.1656 2007.1364 14.6 1 3 0.64 +1Score > 29 indicates identity
Score > 14 indicates homology
DDLLIQALTVAVQVPQRK + Deamidated (NQ)
4125 689.6176 2754.4413 2754.4123 10.5 1 3 1 +1Score > 36 indicates identity
Score > 16 indicates homology
LSGARDPIMLYISGGNTQVIAHLNGR + 2 Deamidated (NQ)
1834 638.8583 1275.7020 1275.6823 15.5 0 3 1 +1Score > 39 indicates identity
Score > 16 indicates homology
GLLNATGPITYR + Deamidated (NQ)
3291 682.3736 2044.0990 2044.0589 19.6 0 3 0.58 +1Score > 37 indicates identity
Score > 14 indicates homology
LGADVSVYPGASIGAGAVIGDR
3962 849.4388 2545.2946 2545.2887 2.33 1 3 0.66 +1Score > 38 indicates identity
Score > 14 indicates homology
LPGTHLDMVRAGIILYGLYPSDE + Oxidation (M)
2728 566.6342 1696.8808 1696.8930 -7.22 1 3 0.91 +1Score > 39 indicates identity
Score > 16 indicates homology
MPSAQAIKSPPVSVNR + Oxidation (M)
4243 966.1475 2895.4207 2895.3776 14.9 0 3 0.75 +1Score > 37 indicates identity
Score > 15 indicates homology
VTDAPCSNMLGMLSGLIADTQISASSTR
3408 1060.5493 2119.0840 2119.0871 -1.45 1 3 0.75 +1Score > 39 indicates identity
Score > 15 indicates homology
VFPISGATGKGMDALLDAVLE + Oxidation (M)
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Not what you expected? Try the select summary.