MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2183 719.9238 1437.8330 1437.8079 17.5 1 5 0.7 +1Score > 35 indicates identity
Score > 16 indicates homology
LVPKVAPQAISSVE + Deamidated (NQ)
2980 614.6528 1840.9366 1840.9723 -19.4 1 5 0.95 +1Score > 39 indicates identity
Score > 17 indicates homology
IANVESSFYLLGYIPR
4399 784.4011 3133.5753 3133.5455 9.51 1 5 0.58 +1Score > 36 indicates identity
Score > 15 indicates homology
ASSLAAVQQLGNLIVFYDHNQISIEGDTK + 3 Deamidated (NQ)
2730 566.9731 1697.8975 1697.8948 1.58 0 5 0.45 +1Score > 39 indicates identity
Score > 14 indicates homology
AGIDALTLADNSLATPR
3210 502.2603 2005.0121 2004.9799 16.0 0 5 0.61 +1Score > 39 indicates identity
Score > 15 indicates homology
MHQQNHLIISVNDSNQK
3658 756.3904 2266.1494 2266.1118 16.6 1 5 0.92 +1Score > 38 indicates identity
Score > 17 indicates homology
AGATTVNIPDTVGYSTPLEFGR + Deamidated (NQ)
3994 645.8130 2579.2229 2579.2173 2.15 1 5 0.95 +1Score > 38 indicates identity
Score > 17 indicates homology
TVSKPVMDNGSGDSDDDKPLAFKR + Deamidated (NQ)
3916 832.0909 2493.2509 2493.2324 7.39 1 5 0.79 +1Score > 38 indicates identity
Score > 16 indicates homology
GLVGRVTGDQMGMLATVMNGLAMR + Oxidation (M)
3006 620.2825 1857.8257 1857.8302 -2.45 1 5 0.63 +1Score > 38 indicates identity
Score > 15 indicates homology
MVDESNAGPVAPAVVADAE + Deamidated (NQ); Oxidation (M)
3388 529.2686 2113.0453 2113.0184 12.7 1 5 0.39 +1Score > 39 indicates identity
Score > 13 indicates homology
TIVGYSPAKSQNMGLMGGLR + 2 Deamidated (NQ); 2 Oxidation (M)
3812 796.0936 2385.2590 2385.2362 9.54 1 5 0.98 +1Score > 36 indicates identity
Score > 17 indicates homology
MTEPVRVQVQTANPYPVIIGR + 2 Deamidated (NQ); Oxidation (M)
4244 966.1843 2895.5311 2895.5011 10.3 1 5 0.87 +1Score > 35 indicates identity
Score > 17 indicates homology
LLVVLNDNDMSISPPVGALSNVLNRAR + 3 Deamidated (NQ); Oxidation (M)
800 834.4469 833.4396 833.4395 0.095 1 5 0.94 +1Score > 42 indicates identity
Score > 17 indicates homology
KDPGYVR
3363 699.6894 2096.0464 2096.0473 -0.44 1 5 0.6 +1Score > 39 indicates identity
Score > 15 indicates homology
NTYVPILSGANMNFDRLR + Oxidation (M)
1036 927.4944 926.4871 926.4960 -9.63 1 5 0.87 +1Score > 39 indicates identity
Score > 16 indicates homology
VLDEILPE
3920 832.4560 2494.3462 2494.3543 -3.27 1 5 0.52 +1Score > 35 indicates identity
Score > 14 indicates homology
NQIIQIKQLYLGTLNSSVAHPR + 2 Deamidated (NQ)
4061 897.4594 2689.3564 2689.3052 19.0 0 5 0.82 +1Score > 37 indicates identity
Score > 16 indicates homology
VDGGITSNDLCMQIQADVLGVDVVR + Oxidation (M)
298 597.3431 596.3358 596.3394 -6.05 0 5 0.65 +1Score > 37 indicates identity
Score > 15 indicates homology
AIHTR
2344 759.9134 1517.8122 1517.8309 -12.3 0 5 0.64 +1Score > 39 indicates identity
Score > 15 indicates homology
LLKPTVMMNLLGR + Deamidated (NQ); 2 Oxidation (M)
4578 886.6952 3542.7517 3542.7096 11.9 1 5 0.62 +1Score > 35 indicates identity
Score > 15 indicates homology
AVQTGIGQLDGINVAIAVMDFQFMGGSMGSVVGEK + Deamidated (NQ); Oxidation (M)
2896 895.5448 1789.0750 1789.0461 16.2 1 4 1 +1Score > 25 indicates identity
Score > 17 indicates homology
GLLSAQRIVVAVAILHE + Deamidated (NQ)
3107 642.3122 1923.9148 1923.9109 2.03 1 4 0.94 +1Score > 40 indicates identity
Score > 17 indicates homology
QQLPNIHTKQCDVADR + 2 Deamidated (NQ)
3108 642.3205 1923.9397 1923.9182 11.1 1 4 0.58 +1Score > 40 indicates identity
Score > 15 indicates homology
ATCIGNNFGAAISMLKQE
2968 612.6611 1834.9615 1834.9537 4.22 1 4 0.62 +1Score > 39 indicates identity
Score > 15 indicates homology
IADAVQQAGATELVVHGR + Deamidated (NQ)
2993 617.6110 1849.8112 1849.8118 -0.36 1 4 0.62 +1Score > 37 indicates identity
Score > 15 indicates homology
EDLDWQLDFANGAQAR + 2 Deamidated (NQ)
2515 801.4207 1600.8268 1600.8573 -19.0 1 4 0.93 +1Score > 40 indicates identity
Score > 17 indicates homology
ALGFEGVQLIGINNR + Deamidated (NQ)
3373 702.3809 2104.1209 2104.1350 -6.72 1 4 0.72 +1Score > 37 indicates identity
Score > 16 indicates homology
LASVLAKLMNLPAPNPDAPR + Deamidated (NQ); Oxidation (M)
4081 676.8485 2703.3649 2703.3174 17.6 0 4 0.4 +1Score > 37 indicates identity
Score > 13 indicates homology
DTPVVAVSLIDTNSFQNQLDQMPR + Oxidation (M)
458 676.3757 675.3684 675.3803 -17.6 0 4 0.89 +1Score > 42 indicates identity
Score > 16 indicates homology
ALVSVST
2475 791.3914 1580.7682 1580.7835 -9.62 1 4 0.88 +1Score > 41 indicates identity
Score > 16 indicates homology
LKNTTPFDPTQYR + Deamidated (NQ)
1216 497.2749 992.5352 992.5212 14.1 0 4 0.81 +1Score > 41 indicates identity
Score > 16 indicates homology
GTLVMDTIK + Oxidation (M)
3603 744.3788 2230.1146 2230.1165 -0.84 0 4 0.82 +1Score > 39 indicates identity
Score > 16 indicates homology
VVAAQDHLTHVVNSVMQPQR + 2 Deamidated (NQ)
3502 724.7299 2171.1679 2171.1620 2.72 1 4 0.99 +1Score > 36 indicates identity
Score > 17 indicates homology
MVLSGAVNKQITALLNQNGAK + 2 Deamidated (NQ)
3531 547.7707 2187.0537 2187.0704 -7.63 1 4 0.9 +1Score > 39 indicates identity
Score > 17 indicates homology
LMGFIQADQSISFQAMRVK + 2 Deamidated (NQ); Oxidation (M)
4069 898.4498 2692.3276 2692.3312 -1.35 1 4 0.99 +1Score > 38 indicates identity
Score > 17 indicates homology
NPIITMINSGARGNISNYVQLAGMR + 3 Deamidated (NQ)
4100 683.5771 2730.2793 2730.2802 -0.34 1 4 0.85 +1Score > 38 indicates identity
Score > 16 indicates homology
MIRSAQQVVAVVMDMFTDVDLLSE + 2 Deamidated (NQ); 2 Oxidation (M)
3182 663.0132 1986.0178 1986.0435 -13.0 1 4 0.84 +1Score > 39 indicates identity
Score > 16 indicates homology
VVAWINDIAASRGPFQSR
3990 857.4548 2569.3426 2569.3486 -2.36 1 4 0.94 +1Score > 36 indicates identity
Score > 17 indicates homology
ALDDSVIDALSDDLNTPKALTLLR + Deamidated (NQ)
4458 1070.8761 3209.6065 3209.6179 -3.56 1 4 1 +1Score > 36 indicates identity
Score > 17 indicates homology
MNLFHLQAPFQATGDQPQAIAQLVNSIEK + Deamidated (NQ)
2524 402.4810 1605.8949 1605.8838 6.89 1 4 0.75 +1Score > 35 indicates identity
Score > 16 indicates homology
RAPQILTPPTQQVR + 2 Deamidated (NQ)
3604 744.7354 2231.1844 2231.2235 -17.6 0 4 1 +1Score > 37 indicates identity
Score > 17 indicates homology
QTDLFSLVPILVTALMDISR
3959 846.0918 2535.2536 2535.2673 -5.40 1 4 0.48 +1Score > 38 indicates identity
Score > 14 indicates homology
SIAAVKTGDADVAVSAGNTGALMAMAK + Oxidation (M)
4259 973.8665 2918.5777 2918.5324 15.5 1 4 1 +1Score > 33 indicates identity
Score > 17 indicates homology
AINHAMAAGVPIVFAINKVDKPTANPDK + Deamidated (NQ); Oxidation (M)
2833 586.6038 1756.7896 1756.8115 -12.5 0 4 0.6 +1Score > 39 indicates identity
Score > 15 indicates homology
SAQVDQQSVQPLPNSR + 4 Deamidated (NQ)
1472 557.2621 1112.5096 1112.4882 19.3 1 4 0.78 +1Score > 39 indicates identity
Score > 16 indicates homology
LEFLMGQME + Oxidation (M)
3637 563.2854 2249.1125 2249.1097 1.25 1 4 0.95 +1Score > 39 indicates identity
Score > 17 indicates homology
NVTIDVTNMPIGTSLKSSQVE + Deamidated (NQ); Oxidation (M)
4009 866.7803 2597.3191 2597.3660 -18.1 1 4 0.81 +1Score > 37 indicates identity
Score > 16 indicates homology
ASQATNQLIQKLLNIIDNINQSR + 3 Deamidated (NQ)
3353 1046.1025 2090.1904 2090.1636 12.8 1 4 1 +1Score > 31 indicates identity
Score > 17 indicates homology
AHAPIAVIADSKIIANSPFR
3593 741.6846 2222.0320 2222.0347 -1.25 1 4 0.61 +1Score > 39 indicates identity
Score > 15 indicates homology
GASGQAVQNMNIMFGLPETAGL + Deamidated (NQ); Oxidation (M)
3852 605.3224 2417.2605 2417.2660 -2.27 0 4 0.66 +1Score > 37 indicates identity
Score > 15 indicates homology
LSAMMVTSFILISVLALGLMYE + Oxidation (M)
4008 648.8088 2591.2061 2591.2214 -5.90 1 4 0.44 +1Score > 38 indicates identity
Score > 13 indicates homology
VWTAVANHVESGIVAAFTMGDLSAE + Deamidated (NQ); Oxidation (M)
1278 506.7421 1011.4696 1011.4695 0.13 1 4 0.84 +1Score > 40 indicates identity
Score > 16 indicates homology
MAVDIEYR + Oxidation (M)
3467 717.0588 2148.1546 2148.1327 10.2 1 4 0.83 +1Score > 36 indicates identity
Score > 16 indicates homology
ITDIQFAQNLNIRGFQLR + 2 Deamidated (NQ)
4188 705.8510 2819.3749 2819.3324 15.1 0 4 0.76 +1Score > 37 indicates identity
Score > 16 indicates homology
AQVSVDMFLFSSQYQDVASLSNLPR + 2 Deamidated (NQ); Oxidation (M)
1635 394.9010 1181.6812 1181.6880 -5.82 0 4 0.84 +1Score > 36 indicates identity
Score > 16 indicates homology
QAVIGAVLAQGR
2144 474.6093 1420.8061 1420.7861 14.1 1 4 0.85 +1Score > 35 indicates identity
Score > 16 indicates homology
VKAGSGFIITCIR
2690 558.2838 1671.8296 1671.8614 -19.0 1 4 0.63 +1Score > 40 indicates identity
Score > 15 indicates homology
VGAMANGDVVLLENVR + Oxidation (M)
3241 1012.5172 2023.0198 2023.0118 3.98 0 4 0.65 +1Score > 40 indicates identity
Score > 15 indicates homology
CLISPMVLAAYAQNQSLK + Deamidated (NQ); Oxidation (M)
1618 588.3332 1174.6518 1174.6346 14.7 0 4 0.82 +1Score > 39 indicates identity
Score > 16 indicates homology
LGPTSALFVDR
2950 606.9645 1817.8717 1817.8472 13.5 0 4 0.66 +1Score > 40 indicates identity
Score > 15 indicates homology
NAVHSWVAATLDYLQE + 2 Deamidated (NQ)
3860 606.5969 2422.3585 2422.3505 3.30 1 4 0.76 +1Score > 31 indicates identity
Score > 16 indicates homology
ALDATLKDMGIILDAVLNIAVPR + Deamidated (NQ)
4258 729.8404 2915.3325 2915.3892 -19.5 0 4 1 +1Score > 36 indicates identity
Score > 17 indicates homology
SPLGSLNSTLMATPSLGLAANQSGPQCLE + 2 Deamidated (NQ)
4336 1010.5618 3028.6636 3028.7181 -18.0 0 4 0.52 +1Score > 31 indicates identity
Score > 14 indicates homology
SQAAGQLLPLPLPTLLVPTLACVMAGLLR + Oxidation (M)
3275 1020.5011 2038.9876 2039.0245 -18.1 1 4 1 +1Score > 39 indicates identity
Score > 17 indicates homology
QGQETVAPNLVAPVMNKPK + 3 Deamidated (NQ); Oxidation (M)
3662 758.3802 2272.1188 2272.1369 -7.97 1 4 0.99 +1Score > 39 indicates identity
Score > 17 indicates homology
SQSTNQIIAIVPAAGIGSRMGAE + 2 Deamidated (NQ)
3316 688.0450 2061.1132 2061.0850 13.7 0 4 0.98 +1Score > 36 indicates identity
Score > 17 indicates homology
MQSQLVSVLQMLSGLGLVR + 3 Deamidated (NQ)
3443 1070.5509 2139.0872 2139.0596 12.9 1 4 0.52 +1Score > 39 indicates identity
Score > 14 indicates homology
KVSGAAAQQAVSYPDNLTYR + Deamidated (NQ)
3732 770.7287 2309.1643 2309.1725 -3.58 1 4 0.92 +1Score > 39 indicates identity
Score > 16 indicates homology
LAFVLSKDGNPDIYVMNLGSR + Deamidated (NQ)
2732 567.3054 1698.8944 1698.8828 6.80 0 4 0.95 +1Score > 39 indicates identity
Score > 17 indicates homology
LAIGYVYTTINTQNK + Deamidated (NQ)
3794 475.4766 2372.3466 2372.3427 1.63 1 4 0.92 +1Score > 30 indicates identity
Score > 16 indicates homology
NGLLASDKYVVIVGLGATGLSVAR
4563 858.6912 3430.7357 3430.7146 6.15 1 4 0.61 +1Score > 35 indicates identity
Score > 15 indicates homology
VAMNPILLKPQGDMTSQVIMLGQAVGTTRAQE + 2 Deamidated (NQ); 2 Oxidation (M)
3306 687.3484 2059.0234 2058.9900 16.2 0 4 0.87 +1Score > 39 indicates identity
Score > 16 indicates homology
TALLNTLMTTAGANFAMMR + 2 Oxidation (M)
4115 916.4681 2746.3825 2746.3735 3.27 0 4 0.78 +1Score > 37 indicates identity
Score > 16 indicates homology
NVNAIQTVSGILQGPFDLGNQLLGMK + 4 Deamidated (NQ); Oxidation (M)
1850 640.8891 1279.7636 1279.7500 10.7 1 4 0.96 +1Score > 33 indicates identity
Score > 17 indicates homology
SVHDILINLKK + Deamidated (NQ)
2824 581.6683 1741.9831 1741.9726 5.99 1 4 0.86 +1Score > 34 indicates identity
Score > 16 indicates homology
VLSLSTYALTSYLRR
3534 1095.0686 2188.1226 2188.1232 -0.23 1 4 1 +1Score > 38 indicates identity
Score > 17 indicates homology
TVLMSPLMVAAAAVAGEIADAR + 2 Oxidation (M)
1717 610.8358 1219.6570 1219.6713 -11.7 1 4 0.67 +1Score > 40 indicates identity
Score > 15 indicates homology
ISPWAKYVTR
259 549.2798 548.2725 548.2806 -14.7 1 4 1 +1Score > 43 indicates identity
Score > 17 indicates homology
AKTTE
392 651.3027 650.2954 650.2912 6.57 0 4 0.91 +1Score > 43 indicates identity
Score > 16 indicates homology
AGDLFE
4066 898.4476 2692.3210 2692.3133 2.86 1 4 0.94 +1Score > 38 indicates identity
Score > 16 indicates homology
RSKPYQSNNFSLAFVPNTPLPQGE + 2 Deamidated (NQ)
2241 738.3585 1474.7024 1474.7012 0.87 1 4 0.68 +1Score > 41 indicates identity
Score > 15 indicates homology
NTEGILSGNATQNR + Deamidated (NQ)
3171 661.6674 1981.9804 1982.0030 -11.4 1 4 0.5 +1Score > 40 indicates identity
Score > 14 indicates homology
MTTLSNLSVDLVGEIFSR + Deamidated (NQ)
4198 709.3671 2833.4393 2833.3956 15.4 1 4 1 +1Score > 37 indicates identity
Score > 17 indicates homology
EQLANLGQPFCYQLLSQVDPVAQSK + Deamidated (NQ)
4430 1061.5272 3181.5598 3181.5682 -2.65 1 4 0.51 +1Score > 36 indicates identity
Score > 14 indicates homology
LAMHGTHNMLNSLAAIGVATQMGIADDKIR + Deamidated (NQ); 2 Oxidation (M)
4321 1003.1667 3006.4783 3006.4354 14.2 1 4 0.85 +1Score > 37 indicates identity
Score > 16 indicates homology
QIDVSATSAQCLYQLAFIFGVQRLME + 3 Deamidated (NQ); Oxidation (M)
2971 919.5047 1836.9948 1837.0282 -18.2 1 4 0.86 +1Score > 37 indicates identity
Score > 16 indicates homology
TARPGVVVGRGGAGIDSLR
3235 674.0096 2019.0070 2018.9796 13.6 1 4 0.49 +1Score > 40 indicates identity
Score > 14 indicates homology
LPQQQQQSFQQLVSARK + 6 Deamidated (NQ)
4132 922.1337 2763.3793 2763.3506 10.4 0 4 0.67 +1Score > 38 indicates identity
Score > 15 indicates homology
NIYAIICGMAAALGAGHNTQAMLLTR + Deamidated (NQ); 2 Oxidation (M)
3815 796.7325 2387.1757 2387.2155 -16.7 0 4 0.7 +1Score > 39 indicates identity
Score > 15 indicates homology
DAVSALVNLGYPQAQAMGAVAAAAK + Deamidated (NQ)
3899 826.0736 2475.1990 2475.2328 -13.7 0 4 0.96 +1Score > 38 indicates identity
Score > 16 indicates homology
DDVAADWAPRPAPPKPALAMAGSR + Oxidation (M)
4044 664.6072 2654.3997 2654.3625 14.0 1 4 0.51 +1Score > 36 indicates identity
Score > 14 indicates homology
TVLNKLCLNTYFTPNLQTISNAK + 2 Deamidated (NQ)
2653 830.9258 1659.8370 1659.8250 7.23 1 4 0.67 +1Score > 41 indicates identity
Score > 15 indicates homology
MSTPVTRTQVPDGVR + Deamidated (NQ); Oxidation (M)
3479 1078.0327 2154.0508 2154.0415 4.32 0 4 0.84 +1Score > 39 indicates identity
Score > 16 indicates homology
VSMAVLTYPDGNYGNLVDAR
3892 617.5861 2466.3153 2466.3482 -13.3 1 4 0.93 +1Score > 35 indicates identity
Score > 16 indicates homology
RPRDIVGLLVNLQAGDILFIDE + Deamidated (NQ)
1024 923.5151 922.5078 922.5236 -17.1 0 4 1 +1Score > 38 indicates identity
Score > 17 indicates homology
AVAPLRPAE
3535 1095.0701 2188.1256 2188.1232 1.14 1 4 0.75 +1Score > 38 indicates identity
Score > 15 indicates homology
TVLMSPLMVAAAAVAGEIADAR + 2 Oxidation (M)
2871 887.4619 1772.9092 1772.9342 -14.1 1 4 0.81 +1Score > 40 indicates identity
Score > 16 indicates homology
TTPANLLNISDMLKNK + Deamidated (NQ)
2015 455.2471 1362.7195 1362.7296 -7.40 1 4 0.61 +1Score > 40 indicates identity
Score > 14 indicates homology
NIPELIAFYQR
3587 555.0691 2216.2473 2216.2304 7.63 1 4 0.95 +1Score > 31 indicates identity
Score > 16 indicates homology
INVYIETNPVINSTVIVSLK + Deamidated (NQ)
4084 902.8149 2705.4229 2705.3694 19.8 1 4 0.44 +1Score > 35 indicates identity
Score > 13 indicates homology
STKIYVGNVPIGGDAPIAVQSMTNTR + Deamidated (NQ); Oxidation (M)
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