MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3522 546.2837 2181.1057 2181.0809 11.3 1 5 0.63 +1Score > 39 indicates identity
Score > 16 indicates homology
MTQTIFVLNGPNLNMLGKR + 3 Deamidated (NQ); 2 Oxidation (M)
377 322.1885 642.3624 642.3561 9.82 1 5 0.59 +1Score > 35 indicates identity
Score > 16 indicates homology
NPRTR
2568 809.3949 1616.7752 1616.7603 9.24 0 5 0.76 +1Score > 40 indicates identity
Score > 17 indicates homology
VQNMLNDALIQVQE + 3 Deamidated (NQ)
3178 662.0552 1983.1438 1983.1193 12.3 1 5 0.41 +1Score > 30 indicates identity
Score > 14 indicates homology
VPWGIVLLLGGGFALAKGSE
4027 879.4155 2635.2247 2635.2258 -0.42 1 5 0.54 +1Score > 37 indicates identity
Score > 15 indicates homology
QMGIESNSIDIMGLQAAYAQLHTE + Oxidation (M)
4177 702.8947 2807.5497 2807.5605 -3.86 1 5 0.74 +1Score > 31 indicates identity
Score > 17 indicates homology
LSNLQAMLPILEAVVQTSKPLVIISE + 2 Deamidated (NQ)
2798 865.4775 1728.9404 1728.9154 14.5 0 5 0.48 +1Score > 38 indicates identity
Score > 15 indicates homology
VTMINAIPMASLDPIK + Oxidation (M)
1698 607.3316 1212.6486 1212.6602 -9.49 1 5 0.99 +1Score > 40 indicates identity
Score > 18 indicates homology
SKLIPTDPITE
1940 663.4166 1324.8186 1324.8078 8.18 1 5 0.95 +1Score > 29 indicates identity
Score > 18 indicates homology
NVVVVAANGKILK + Deamidated (NQ)
2604 409.9793 1635.8881 1635.8555 19.9 1 5 0.96 +1Score > 37 indicates identity
Score > 18 indicates homology
LLVCAPRFYAPSSR
2864 885.0130 1768.0114 1768.0207 -5.21 1 5 0.43 +1Score > 33 indicates identity
Score > 14 indicates homology
AGRTAAVVLTQLAAVAQK + Deamidated (NQ)
1435 546.8091 1091.6036 1091.6226 -17.4 0 5 0.38 +1Score > 39 indicates identity
Score > 14 indicates homology
LGNILVAYTK + Deamidated (NQ)
2481 792.4004 1582.7862 1582.8103 -15.2 1 5 0.85 +1Score > 40 indicates identity
Score > 17 indicates homology
YTIHSQLEHLQSK
4685 1044.0596 4172.2093 4172.1764 7.88 1 5 0.4 +1Score > 30 indicates identity
Score > 14 indicates homology
VAVSLFLLSLIGSLMDFQTLCHTSVLVVMTVPAFYER + Oxidation (M)
2318 755.9515 1509.8884 1509.9017 -8.81 1 5 0.53 +1Score > 32 indicates identity
Score > 15 indicates homology
ILVQIIKEPTLNK + 2 Deamidated (NQ)
3238 674.3516 2020.0330 2020.0153 8.74 0 5 0.9 +1Score > 39 indicates identity
Score > 17 indicates homology
QVAFASTNIIPGLDFSPDK + Deamidated (NQ)
3746 1162.5878 2323.1610 2323.1453 6.78 1 5 0.98 +1Score > 39 indicates identity
Score > 18 indicates homology
QFNINIPIATVMTDYRMHK + 2 Oxidation (M)
2984 615.0217 1842.0433 1842.0251 9.88 0 5 0.45 +1Score > 34 indicates identity
Score > 14 indicates homology
IYLQNGIQSIIDVPIR + Deamidated (NQ)
2351 763.4257 1524.8368 1524.8147 14.5 1 5 0.69 +1Score > 37 indicates identity
Score > 16 indicates homology
VAQQGGLKLIPQDR + 3 Deamidated (NQ)
2622 548.6375 1642.8907 1642.8580 19.9 1 5 0.8 +1Score > 38 indicates identity
Score > 17 indicates homology
AFTPFPPRQPTASAR
349 623.3661 622.3588 622.3690 -16.4 0 5 1 +1Score > 33 indicates identity
Score > 18 indicates homology
SLPPLP
983 455.2271 908.4396 908.4352 4.92 1 5 0.73 +1Score > 41 indicates identity
Score > 16 indicates homology
YGGTIENR
2658 555.9763 1664.9071 1664.9138 -4.03 1 5 0.44 +1Score > 37 indicates identity
Score > 14 indicates homology
TVLGFFGITDIEVVR
3632 750.0939 2247.2599 2247.2362 10.5 1 5 0.42 +1Score > 32 indicates identity
Score > 14 indicates homology
NPSLHLVLVGQSSLLEQLVAK + 3 Deamidated (NQ)
3731 578.2957 2309.1537 2309.1249 12.5 0 5 0.99 +1Score > 39 indicates identity
Score > 18 indicates homology
AMALADGNAPYTLLAQYLNGIE + Deamidated (NQ)
3338 696.0099 2085.0079 2085.0010 3.31 1 5 0.91 +1Score > 39 indicates identity
Score > 17 indicates homology
ILCAYASLVSQDVEMLQK + 2 Deamidated (NQ); Oxidation (M)
3930 837.1094 2508.3064 2508.3298 -9.34 1 5 0.35 +1Score > 37 indicates identity
Score > 13 indicates homology
VVWLEINTQPGMTPTSLVPDIAK
1336 346.8706 1037.5900 1037.6094 -18.7 1 5 0.82 +1Score > 35 indicates identity
Score > 17 indicates homology
AAPNAGLRIR
795 417.2205 832.4264 832.4330 -7.92 1 5 0.85 +1Score > 43 indicates identity
Score > 17 indicates homology
EIAFQPK + Deamidated (NQ)
2365 765.9270 1529.8394 1529.8136 16.9 1 5 0.71 +1Score > 39 indicates identity
Score > 16 indicates homology
LTRLNHFLVNMR + Deamidated (NQ); Oxidation (M)
3120 484.5208 1934.0541 1934.0697 -8.09 1 5 0.94 +1Score > 36 indicates identity
Score > 17 indicates homology
ALLQAGADKVSINTAAVHR
2421 312.3889 1556.9081 1556.8827 16.3 1 5 0.8 +1Score > 33 indicates identity
Score > 17 indicates homology
KIIAIFQPHTFSR
2956 456.7455 1822.9529 1822.9173 19.5 1 5 0.88 +1Score > 39 indicates identity
Score > 17 indicates homology
SGHNVDVTLEPGGSRIGK + Deamidated (NQ)
3716 769.3721 2305.0945 2305.1260 -13.7 1 5 0.46 +1Score > 39 indicates identity
Score > 14 indicates homology
MQENSPLLQLQNVGYLAGDAK + Deamidated (NQ); Oxidation (M)
4590 894.4971 3573.9593 3573.9554 1.09 1 5 0.51 +1Score > 29 indicates identity
Score > 15 indicates homology
LVFGVTLLQGAMLTGVATFLILMLQNRGQKPLE + 3 Deamidated (NQ)
4566 687.5551 3432.7391 3432.7170 6.45 1 5 0.93 +1Score > 35 indicates identity
Score > 17 indicates homology
ISGSVHYDNVAPSYLGGMQLIINKNDIVCQK
2840 587.6769 1760.0089 1759.9846 13.8 1 5 0.46 +1Score > 32 indicates identity
Score > 14 indicates homology
KATQGIYPLQHVHIR
414 334.2114 666.4082 666.4177 -14.2 1 5 0.81 +1Score > 29 indicates identity
Score > 17 indicates homology
VKHVGK
3809 794.7580 2381.2522 2381.2155 15.4 1 5 1 +1Score > 37 indicates identity
Score > 18 indicates homology
GTGLDVVWQPLLSVWLEDEPK + Deamidated (NQ)
1007 459.2195 916.4244 916.4250 -0.61 0 5 0.94 +1Score > 40 indicates identity
Score > 17 indicates homology
DGDAAIAQR + Deamidated (NQ)
3492 1084.5641 2167.1136 2167.0990 6.77 1 5 0.87 +1Score > 38 indicates identity
Score > 17 indicates homology
QAQRAMIGNHTAIGAIMVQR + 2 Deamidated (NQ)
4231 961.1349 2880.3829 2880.3409 14.6 1 5 0.78 +1Score > 37 indicates identity
Score > 17 indicates homology
HMDQLQQNVSIVTQQKMQPIYSVK + 6 Deamidated (NQ); 2 Oxidation (M)
2272 498.9251 1493.7535 1493.7725 -12.8 1 5 0.79 +1Score > 41 indicates identity
Score > 17 indicates homology
IKLNTNDNYTGLK + Deamidated (NQ)
3276 680.6713 2038.9921 2038.9822 4.82 1 5 0.68 +1Score > 40 indicates identity
Score > 16 indicates homology
GVFMDQALEILPSDYWR
2567 539.6325 1615.8757 1615.8967 -13.0 1 5 0.43 +1Score > 39 indicates identity
Score > 14 indicates homology
TINSGDVINMALLKK
2889 596.6596 1786.9570 1786.9214 19.9 1 5 0.82 +1Score > 39 indicates identity
Score > 17 indicates homology
QPYRILGGGSNLVVADE
3057 629.0088 1884.0046 1883.9966 4.24 1 5 0.56 +1Score > 38 indicates identity
Score > 15 indicates homology
QGVALVLLAHHQRDQAE
4276 734.3876 2933.5213 2933.5168 1.53 1 5 0.4 +1Score > 35 indicates identity
Score > 14 indicates homology
SVRDLALLMDAVAGPDPLDATSLDLPPR + Oxidation (M)
4067 898.4489 2692.3249 2692.3742 -18.3 0 5 0.86 +1Score > 38 indicates identity
Score > 17 indicates homology
TTTISMLTGLYTPTSGTAHVAGLDIR + Oxidation (M)
4088 903.8199 2708.4379 2708.4346 1.21 1 5 0.69 +1Score > 35 indicates identity
Score > 16 indicates homology
RLMAYSTILNIGYVLIGVILPNQE + 3 Deamidated (NQ); Oxidation (M)
1375 1061.5403 1060.5330 1060.5223 10.1 1 5 0.58 +1Score > 42 indicates identity
Score > 15 indicates homology
KLDQLGVCE
2162 714.4249 1426.8352 1426.8143 14.6 1 5 0.72 +1Score > 34 indicates identity
Score > 16 indicates homology
IQQLAVSGLKVNR + 2 Deamidated (NQ)
2603 409.7292 1634.8877 1634.8628 15.2 1 5 0.63 +1Score > 37 indicates identity
Score > 16 indicates homology
ISIDDFGTGLSNLKR
2685 557.3375 1668.9907 1668.9847 3.55 1 5 0.95 +1Score > 30 indicates identity
Score > 17 indicates homology
ALSLAKPIIQMLKNK + 2 Deamidated (NQ)
3382 1056.0524 2110.0902 2110.1133 -10.9 1 5 0.92 +1Score > 38 indicates identity
Score > 17 indicates homology
AGECPYQVSLPPLTIQLPK
1526 567.3408 1132.6670 1132.6856 -16.3 1 5 0.64 +1Score > 35 indicates identity
Score > 16 indicates homology
KLSNIVFAIK + Deamidated (NQ)
3744 774.3674 2320.0804 2320.1198 -17.0 1 5 0.69 +1Score > 38 indicates identity
Score > 16 indicates homology
FSRSILWDYFSLVLTNACE
3871 813.4474 2437.3204 2437.2747 18.7 0 5 0.7 +1Score > 34 indicates identity
Score > 16 indicates homology
RPLMGNIAAQLADLAVVTSDNPR + Oxidation (M)
3944 840.4350 2518.2832 2518.2904 -2.85 1 5 1 +1Score > 38 indicates identity
Score > 17 indicates homology
SGIPVMGHIGLTPQFVNRFGGYR + Oxidation (M)
2969 919.4417 1836.8688 1836.9040 -19.1 1 5 0.83 +1Score > 40 indicates identity
Score > 17 indicates homology
LMRDPVVVATGQTYDR + Deamidated (NQ); Oxidation (M)
3666 758.7100 2273.1082 2273.1072 0.44 1 5 1 +1Score > 39 indicates identity
Score > 17 indicates homology
ISHLGYSEALMSTMVTNFLK + 2 Oxidation (M)
4141 926.1212 2775.3418 2775.3459 -1.49 1 5 0.61 +1Score > 38 indicates identity
Score > 15 indicates homology
GAEQIGLQLMDSFTPIQPIMIGDAGR + 2 Deamidated (NQ); Oxidation (M)
1003 916.4795 915.4722 915.4549 18.9 1 5 0.96 +1Score > 42 indicates identity
Score > 17 indicates homology
IDKTVDPE
3642 563.8160 2251.2349 2251.2590 -10.7 1 5 0.45 +1Score > 34 indicates identity
Score > 14 indicates homology
VRLVDNGAVAFIPASFLHAVR
712 395.7088 789.4030 789.4167 -17.3 1 5 0.93 +1Score > 42 indicates identity
Score > 17 indicates homology
MVPSGRK + Oxidation (M)
3423 710.4188 2128.2346 2128.2005 16.0 1 5 1 +1Score > 28 indicates identity
Score > 17 indicates homology
FDTIGGLVLTRVGSVPAIGTR
4352 764.4034 3053.5845 3053.5777 2.23 1 5 0.58 +1Score > 35 indicates identity
Score > 15 indicates homology
TCIVIAHRLSTIQNADIIAVMAQGVVIE + 3 Deamidated (NQ); Oxidation (M)
4616 747.3972 3731.9496 3732.0173 -18.1 1 5 0.48 +1Score > 32 indicates identity
Score > 14 indicates homology
TPSLGFTVTLVLLNSLAFLLMAIIYTKLYCNLE + Deamidated (NQ)
767 411.2446 820.4746 820.4694 6.37 0 5 0.52 +1Score > 37 indicates identity
Score > 15 indicates homology
IGILNYK + Deamidated (NQ)
3369 1051.0114 2100.0082 2100.0415 -15.8 1 5 0.86 +1Score > 39 indicates identity
Score > 17 indicates homology
LLSQPFNSSTPVLYYNKE + Deamidated (NQ)
4152 696.1124 2780.4205 2780.4749 -19.5 1 5 0.61 +1Score > 37 indicates identity
Score > 15 indicates homology
SFIKLLNVSVNYFLGDPSQLQLQR + 2 Deamidated (NQ)
2501 532.9811 1595.9215 1595.9181 2.12 1 5 0.59 +1Score > 34 indicates identity
Score > 15 indicates homology
QIARMGRPLLIIAE + Oxidation (M)
3412 1061.0509 2120.0872 2120.0870 0.100 1 5 0.48 +1Score > 39 indicates identity
Score > 14 indicates homology
RGALQAIDQMSLFRPLCK + Deamidated (NQ); Oxidation (M)
4071 898.4511 2692.3315 2692.3391 -2.84 1 5 0.62 +1Score > 38 indicates identity
Score > 15 indicates homology
DVAAGGQGAPLMPAFHLAILGSANENR + Oxidation (M)
3596 741.6856 2222.0350 2221.9950 18.0 1 5 0.62 +1Score > 39 indicates identity
Score > 15 indicates homology
VRSFTQDDAHIFCTPGQIE + 2 Deamidated (NQ)
3357 1047.5380 2093.0614 2093.0252 17.3 0 5 0.43 +1Score > 39 indicates identity
Score > 14 indicates homology
MSDAVPQVGSQFGPYQLLR + Deamidated (NQ)
4036 882.0964 2643.2674 2643.3070 -15.0 1 5 0.47 +1Score > 38 indicates identity
Score > 14 indicates homology
MLSAAMDFIIMQQSRTGSQLLLR + 2 Deamidated (NQ); 2 Oxidation (M)
4520 830.4297 3317.6897 3317.6893 0.11 0 5 1 +1Score > 35 indicates identity
Score > 17 indicates homology
IVILALLQHDNIIAYYNHFMDNTTLLIE + 2 Deamidated (NQ); Oxidation (M)
2768 429.2148 1712.8301 1712.8403 -5.98 0 5 0.77 +1Score > 40 indicates identity
Score > 16 indicates homology
VQDVVLSDGAADHMLK + Oxidation (M)
2912 601.6087 1801.8043 1801.7910 7.37 0 5 0.81 +1Score > 38 indicates identity
Score > 16 indicates homology
MQHDSVMMGGPVQVDR + Oxidation (M)
3639 750.7263 2249.1571 2249.1474 4.31 1 5 0.49 +1Score > 38 indicates identity
Score > 14 indicates homology
TLMLNDRIQNPDALQHVLR + 3 Deamidated (NQ)
2355 509.6394 1525.8964 1525.8716 16.3 1 5 1 +1Score > 32 indicates identity
Score > 17 indicates homology
VVLSQVSLAISPGEK
2525 804.4459 1606.8772 1606.8692 5.02 1 5 0.38 +1Score > 37 indicates identity
Score > 13 indicates homology
ALAHNLGFPRIGADR
4016 873.0865 2616.2377 2616.2490 -4.32 1 5 0.9 +1Score > 38 indicates identity
Score > 17 indicates homology
SLGQRHYDVQLIGGMVLNDGNIAE + 2 Deamidated (NQ); Oxidation (M)
2532 805.9440 1609.8734 1609.8828 -5.80 0 5 0.96 +1Score > 38 indicates identity
Score > 17 indicates homology
YDQLPLLPAPGTVAR
3850 806.7535 2417.2387 2417.2186 8.29 1 5 0.87 +1Score > 38 indicates identity
Score > 17 indicates homology
AIQLANDSQYGLAGAVLSNDLER
1662 599.3275 1196.6404 1196.6414 -0.82 1 5 0.72 +1Score > 39 indicates identity
Score > 16 indicates homology
RSVWTAPQPR
1354 1050.5598 1049.5525 1049.5506 1.88 0 5 0.7 +1Score > 41 indicates identity
Score > 16 indicates homology
FTVSNVNLR + Deamidated (NQ)
2946 454.2391 1812.9273 1812.9006 14.7 0 5 0.65 +1Score > 40 indicates identity
Score > 15 indicates homology
DLNVLAYNDLVHSPSR + Deamidated (NQ)
3125 972.0412 1942.0678 1942.0452 11.7 0 5 0.86 +1Score > 35 indicates identity
Score > 17 indicates homology
LILIPNGVQIFFVNPAGE + 2 Deamidated (NQ)
661 384.7071 767.3996 767.3926 9.21 1 5 0.63 +1Score > 37 indicates identity
Score > 15 indicates homology
YSASGKR
1958 334.4386 1333.7253 1333.7064 14.2 1 5 0.89 +1Score > 39 indicates identity
Score > 17 indicates homology
MSIVNFKGNPVK + Deamidated (NQ)
2647 552.9512 1655.8318 1655.8342 -1.44 0 5 0.75 +1Score > 40 indicates identity
Score > 16 indicates homology
CSYVVTNGLGVNVFK
3232 1010.0078 2018.0010 2017.9705 15.2 1 5 0.53 +1Score > 40 indicates identity
Score > 15 indicates homology
APTTTATAAAEQAPPPQHTR + 2 Deamidated (NQ)
3197 666.3319 1995.9739 1995.9942 -10.2 1 5 0.95 +1Score > 40 indicates identity
Score > 17 indicates homology
SYQFEFSHAAILQTILE
772 411.7309 821.4472 821.4395 9.38 1 5 0.79 +1Score > 38 indicates identity
Score > 16 indicates homology
TFNGVKR + Deamidated (NQ)
2712 563.2993 1686.8761 1686.9087 -19.3 1 5 0.76 +1Score > 40 indicates identity
Score > 16 indicates homology
AALIALCGDTLSAKQR
3451 537.0529 2144.1825 2144.1446 17.7 1 5 0.78 +1Score > 34 indicates identity
Score > 16 indicates homology
TRILDIGCGSGLLGLMLAQR + Deamidated (NQ)
2294 750.9471 1499.8796 1499.9035 -15.9 1 5 0.92 +1Score > 32 indicates identity
Score > 17 indicates homology
SIRLIIISGLSGSGK
3718 769.7056 2306.0950 2306.1213 -11.4 1 5 1 +1Score > 39 indicates identity
Score > 17 indicates homology
FALVGDVGGTNARLALCDLASGE + Deamidated (NQ)
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