MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 101–200 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
827 423.2307 844.4468 844.4402 7.82 1 29 0.24 +1Score > 42 indicates identity
Score > 35 indicates homology		 LQNKADR + Deamidated (NQ)
367 631.3680 630.3607 630.3588 3.04 1 29 0.68 +1Score > 44 indicates identity
Score > 40 indicates homology		 QIEIK + Deamidated (NQ)
1097 473.7556 945.4966 945.5131 -17.4 1 29 0.69 +1Score > 42 indicates identity
Score > 40 indicates homology		 VNSLEASVK
1741 614.8386 1227.6626 1227.6823 -16.0 0 29 0.49 +1Score > 40 indicates identity
Score > 38 indicates homology		 IDVGGSAAVLGAAK
886 433.7052 865.3958 865.3851 12.4 1 29 0.83 +1Score > 41 indicates identity
Score > 40 indicates homology		 GMNLKTGE + Deamidated (NQ); Oxidation (M)
1359 527.7215 1053.4284 1053.4251 3.21 0 29 0.33 +1Score > 37 indicates identity
Score > 36 indicates homology		 QFNQSVSNE + 2 Deamidated (NQ)
621 377.2173 752.4200 752.4181 2.62 0 29 0.13 +1Score > 38 indicates identity
Score > 32 indicates homology		 ALDVHAK
1448 549.3159 1096.6172 1096.6063 10.0 1 29 0.38 +1Score > 37 indicates identity LPDIGPKMAR
1748 615.3661 1228.7176 1228.7027 12.2 1 28 0.33 +1Score > 36 indicates identity QLSEGLLALSAK
1470 371.5468 1111.6186 1111.6349 -14.7 0 28 0.14 +1Score > 38 indicates identity
Score > 32 indicates homology		 QLGANAVIGIR + Deamidated (NQ)
2424 520.6165 1558.8277 1558.8144 8.54 0 28 0.23 +1Score > 40 indicates identity
Score > 34 indicates homology		 LSFLPSHFIQVNR + 2 Deamidated (NQ)
1739 614.8384 1227.6622 1227.6459 13.3 0 28 0.89 +1Score > 40 indicates identity QLNVVGAQLQR + 3 Deamidated (NQ)
1092 472.2662 942.5178 942.5134 4.69 0 28 0.74 +1Score > 41 indicates identity
Score > 39 indicates homology		 QVQIDIAR + Deamidated (NQ)
1737 614.8381 1227.6616 1227.6459 12.8 1 28 0.42 +1Score > 40 indicates identity
Score > 37 indicates homology		 QAQQLLELQR + 2 Deamidated (NQ)
1736 614.8380 1227.6614 1227.6710 -7.78 1 28 0.61 +1Score > 40 indicates identity
Score > 38 indicates homology		 NILNEAQIIAK + 2 Deamidated (NQ)
470 685.4378 684.4305 684.4282 3.36 0 28 0.64 +1Score > 39 indicates identity ALAIAAR
957 449.7014 897.3882 897.3828 6.06 0 28 0.44 +1Score > 38 indicates identity
Score > 37 indicates homology		 SQGSSQFR + 2 Deamidated (NQ)
832 848.3925 847.3852 847.3937 -9.96 1 28 0.56 +1Score > 41 indicates identity
Score > 38 indicates homology		 DDTWRR
1029 308.8478 923.5216 923.5188 2.95 0 28 0.45 +1Score > 37 indicates identity DAHLILSR
322 307.7039 613.3932 613.3911 3.43 0 28 0.56 +1Score > 40 indicates identity
Score > 38 indicates homology		 IGGVLR
1828 425.5439 1273.6099 1273.6350 -19.7 1 28 0.78 +1Score > 41 indicates identity
Score > 39 indicates homology		 AACGWRAVDLR
256 544.3477 543.3404 543.3380 4.38 0 27 0.8 +1Score > 44 indicates identity
Score > 39 indicates homology		 IAGGVK
1628 1181.7236 1180.7163 1180.7067 8.13 1 27 0.18 +1Score > 32 indicates identity DVVPVLIEGLK
429 671.3748 670.3675 670.3650 3.80 0 27 0.45 +1Score > 36 indicates identity DAGIPAK
1668 399.8966 1196.6680 1196.6877 -16.5 1 27 0.65 +1Score > 38 indicates identity
Score > 38 indicates homology		 DRIVTPLNIR + Deamidated (NQ)
1267 337.5273 1009.5601 1009.5570 3.08 1 27 0.13 +1Score > 37 indicates identity
Score > 31 indicates homology		 AWRLHTAR
792 416.2474 830.4802 830.4861 -7.10 1 27 0.25 +1Score > 40 indicates identity
Score > 34 indicates homology		 TIESLIR
670 388.1915 774.3684 774.3733 -6.21 1 27 0.27 +1Score > 42 indicates identity
Score > 34 indicates homology		 GGSRDQR
599 371.7464 741.4782 741.4749 4.58 0 27 0.57 +1Score > 37 indicates identity LAQLIGK
1740 614.8386 1227.6626 1227.6459 13.7 1 27 0.81 +1Score > 40 indicates identity
Score > 39 indicates homology		 QAQQLLELQR + 2 Deamidated (NQ)
1615 587.2928 1172.5710 1172.5673 3.20 1 27 0.14 +1Score > 41 indicates identity
Score > 31 indicates homology		 QQQLQLQRE + 3 Deamidated (NQ)
724 398.7160 795.4174 795.4126 6.04 0 27 0.14 +1Score > 38 indicates identity
Score > 31 indicates homology		 ASITNYK
1921 660.8288 1319.6430 1319.6622 -14.5 1 27 0.35 +1Score > 41 indicates identity
Score > 35 indicates homology		 INQQAFQWKR + 2 Deamidated (NQ)
2285 500.2950 1497.8632 1497.8667 -2.38 1 27 0.061 +1Score > 35 indicates identity
Score > 27 indicates homology		 RVFQAAQALGIPVK + Deamidated (NQ)
1732 307.1693 1224.6481 1224.6462 1.53 0 27 0.25 +1Score > 40 indicates identity
Score > 33 indicates homology		 APTNASKPVSPR + Deamidated (NQ)
320 307.6868 613.3590 613.3548 7.00 0 27 0.31 +1Score > 34 indicates identity VTAPAR
905 437.7535 873.4924 873.4920 0.56 0 27 0.93 +1Score > 41 indicates identity
Score > 39 indicates homology		 AQITTLAR + Deamidated (NQ)
1441 547.7851 1093.5556 1093.5516 3.70 0 27 0.5 +1Score > 41 indicates identity
Score > 36 indicates homology		 AATNGAPGVAHK + Deamidated (NQ)
653 382.7339 763.4532 763.4480 6.92 0 27 0.082 +1Score > 35 indicates identity
Score > 28 indicates homology		 IIDYIK
357 315.1853 628.3560 628.3544 2.57 0 27 0.64 +1Score > 37 indicates identity LGGGTPK
1044 464.7131 927.4116 927.3974 15.4 1 26 0.12 +1Score > 38 indicates identity
Score > 30 indicates homology		 EFFDQNK + Deamidated (NQ)
1172 486.7659 971.5172 971.5148 2.49 0 26 0.65 +1Score > 42 indicates identity
Score > 37 indicates homology		 SAGLQVQNR
237 516.3044 515.2971 515.2955 3.18 0 26 0.22 +1Score > 47 indicates identity
Score > 32 indicates homology		 ALAQL + Deamidated (NQ)
1158 485.2732 968.5318 968.5192 13.0 0 26 0.24 +1Score > 38 indicates identity
Score > 33 indicates homology		 IVPGFSHGR
2097 699.8889 1397.7632 1397.7514 8.47 1 26 0.42 +1Score > 39 indicates identity
Score > 35 indicates homology		 VLALLNDNLQRE + Deamidated (NQ)
906 437.7541 873.4936 873.5032 -10.9 1 26 0.48 +1Score > 42 indicates identity
Score > 36 indicates homology		 ISLTRQR + Deamidated (NQ)
676 388.6939 775.3732 775.3864 -17.0 0 26 0.54 +1Score > 43 indicates identity
Score > 36 indicates homology		 WNSALGK + Deamidated (NQ)
932 444.2591 886.5036 886.4872 18.5 0 26 0.91 +1Score > 42 indicates identity
Score > 38 indicates homology		 QNSGVILR + Deamidated (NQ)
1937 663.3446 1324.6746 1324.6524 16.8 1 26 0.1 +1Score > 40 indicates identity
Score > 29 indicates homology		 FEASQYTRPAR
1475 557.8109 1113.6072 1113.6030 3.85 1 26 0.46 +1Score > 41 indicates identity
Score > 35 indicates homology		 IAGTEAGISAPK
937 446.2457 890.4768 890.4610 17.8 0 26 0.76 +1Score > 41 indicates identity
Score > 37 indicates homology		 ALAGFDAAR
972 451.2735 900.5324 900.5392 -7.55 1 26 0.6 +1Score > 39 indicates identity
Score > 36 indicates homology		 VIDAIKSR
500 351.6895 701.3644 701.3708 -9.04 1 26 0.46 +1Score > 44 indicates identity
Score > 35 indicates homology		 IEGQVR + Deamidated (NQ)
1694 606.3467 1210.6788 1210.6822 -2.77 1 26 0.47 +1Score > 37 indicates identity
Score > 35 indicates homology		 QSILLRYYR
375 321.7195 641.4244 641.4224 3.16 0 26 0.43 +1Score > 35 indicates identity IGAILR
272 561.3040 560.2967 560.2918 8.75 0 26 0.41 +1Score > 44 indicates identity
Score > 35 indicates homology		 DATVR
455 338.1936 674.3726 674.3711 2.26 0 26 0.75 +1Score > 42 indicates identity
Score > 37 indicates homology		 TIGSAAR
834 424.7002 847.3858 847.3937 -9.22 1 26 0.12 +1Score > 41 indicates identity
Score > 29 indicates homology		 DDTWRR
448 337.6781 673.3416 673.3395 3.22 1 26 0.11 +1Score > 44 indicates identity
Score > 29 indicates homology		 AEIDAR
439 336.7121 671.4096 671.3966 19.4 1 26 0.49 +1Score > 44 indicates identity
Score > 35 indicates homology		 GVVEIR
3609 745.4366 2233.2880 2233.3232 -15.8 0 26 0.11 +1Score > 28 indicates identity MVVLPVAIALILGGALGNLIDR + Oxidation (M)
386 323.6920 645.3694 645.3632 9.67 0 26 0.22 +1Score > 43 indicates identity
Score > 32 indicates homology		 AGMIVR
2234 490.9286 1469.7640 1469.7627 0.88 1 26 0.097 +1Score > 40 indicates identity
Score > 28 indicates homology		 HSVYGGVVPELASR
567 364.2222 726.4298 726.4276 3.14 1 25 0.48 +1Score > 36 indicates identity
Score > 35 indicates homology		 IPAGELK
1369 529.7914 1057.5682 1057.5768 -8.06 0 25 0.48 +1Score > 42 indicates identity
Score > 35 indicates homology		 NAATDGVVLAK
1888 651.3584 1300.7022 1300.7139 -8.98 0 25 0.2 +1Score > 41 indicates identity
Score > 31 indicates homology		 WIASGLTDVLAR
271 561.3021 560.2948 560.2918 5.35 0 25 0.3 +1Score > 44 indicates identity
Score > 33 indicates homology		 DATVR
642 381.2070 760.3994 760.3967 3.68 1 25 0.96 +1Score > 43 indicates identity
Score > 38 indicates homology		 AQTEALK + Deamidated (NQ)
674 775.4359 774.4286 774.4348 -7.95 1 25 0.74 +1Score > 41 indicates identity
Score > 37 indicates homology		 NGISRTK
1050 310.5125 928.5157 928.5342 -19.9 0 25 0.24 +1Score > 43 indicates identity
Score > 32 indicates homology		 IIQVVSNR + Deamidated (NQ)
584 366.2195 730.4244 730.4337 -12.7 0 25 0.14 +1Score > 43 indicates identity
Score > 29 indicates homology		 VATTALR
469 343.2225 684.4304 684.4282 3.25 0 25 0.32 +1Score > 39 indicates identity
Score > 33 indicates homology		 LAALAAR
613 375.2215 748.4284 748.4232 7.05 0 25 0.33 +1Score > 38 indicates identity
Score > 33 indicates homology		 IGGIFSR
615 375.7127 749.4108 749.4072 4.89 0 25 0.79 +1Score > 42 indicates identity
Score > 37 indicates homology		 IFTNVR + Deamidated (NQ)
1095 473.2495 944.4844 944.4814 3.18 1 25 0.77 +1Score > 42 indicates identity
Score > 37 indicates homology		 LDQLAAEGK + Deamidated (NQ)
540 713.4983 712.4910 712.4847 8.88 0 25 0.43 +1Score > 34 indicates identity AGVLLLK
2052 345.1904 1376.7325 1376.7122 14.7 0 25 0.59 +1Score > 39 indicates identity
Score > 35 indicates homology		 ATLTLAMADWLR + Oxidation (M)
409 330.2090 658.4034 658.4126 -13.9 1 25 0.4 +1Score > 39 indicates identity
Score > 34 indicates homology		 IGSKVR
1735 614.8377 1227.6608 1227.6459 12.2 1 25 0.33 +1Score > 40 indicates identity
Score > 33 indicates homology		 QAQQLLELQR + 2 Deamidated (NQ)
518 352.2223 702.4300 702.4276 3.51 1 25 0.31 +1Score > 38 indicates identity
Score > 32 indicates homology		 KIDVTK
1049 310.5122 928.5148 928.4978 18.3 1 25 0.37 +1Score > 41 indicates identity
Score > 33 indicates homology		 LLEVNGQR + Deamidated (NQ)
791 416.2472 830.4798 830.4749 5.94 1 25 0.63 +1Score > 40 indicates identity
Score > 35 indicates homology		 TQVELLK + Deamidated (NQ)
909 438.7524 875.4902 875.4865 4.30 1 25 0.21 +1Score > 40 indicates identity
Score > 31 indicates homology		 LFAEQIR
1646 594.3206 1186.6266 1186.6346 -6.69 1 25 0.75 +1Score > 41 indicates identity
Score > 36 indicates homology		 AKASLIAQAWE
266 558.3630 557.3557 557.3537 3.66 0 25 0.87 +1Score > 40 indicates identity
Score > 37 indicates homology		 AGAVLK
1246 502.2816 1002.5486 1002.5346 14.1 0 25 0.5 +1Score > 41 indicates identity
Score > 34 indicates homology		 VTNVAGINSK + Deamidated (NQ)
3711 768.3838 2302.1296 2302.1176 5.19 1 25 0.22 +1Score > 39 indicates identity
Score > 31 indicates homology		 SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ)
1791 625.3295 1248.6444 1248.6350 7.56 1 25 0.67 +1Score > 41 indicates identity
Score > 36 indicates homology		 ATFLQVRVQGE + 2 Deamidated (NQ)
790 416.2469 830.4792 830.4749 5.22 1 25 0.28 +1Score > 40 indicates identity
Score > 32 indicates homology		 TLEQIVK + Deamidated (NQ)
812 841.5172 840.5099 840.5069 3.62 1 25 0.64 +1Score > 35 indicates identity KIDQIPK
1349 1045.5464 1044.5391 1044.5451 -5.74 0 25 0.083 +1Score > 42 indicates identity
Score > 27 indicates homology		 QLLLDTADR + Deamidated (NQ)
1692 606.3450 1210.6754 1210.6670 6.98 0 25 0.56 +1Score > 38 indicates identity
Score > 35 indicates homology		 QVTINDLPVGR
402 656.4165 655.4092 655.4017 11.5 0 25 0.33 +1Score > 33 indicates identity AISIPR
1341 521.8002 1041.5858 1041.5931 -6.93 1 25 0.98 +1Score > 39 indicates identity
Score > 37 indicates homology		 IRAALDDLR
973 452.2449 902.4752 902.4821 -7.64 0 25 0.9 +1Score > 43 indicates identity
Score > 37 indicates homology		 VTAGLDTAR
969 301.1701 900.4885 900.5029 -16.0 1 25 0.27 +1Score > 42 indicates identity
Score > 32 indicates homology		 AEVLSNIR
1346 522.3083 1042.6020 1042.5923 9.31 0 25 0.071 +1Score > 39 indicates identity
Score > 26 indicates homology		 VFPAININR
1075 470.7530 939.4914 939.5025 -11.8 0 25 0.065 +1Score > 39 indicates identity
Score > 25 indicates homology		 SLNVLQHK + 2 Deamidated (NQ)
1641 593.3335 1184.6524 1184.6401 10.5 1 25 0.67 +1Score > 40 indicates identity
Score > 35 indicates homology		 IQELINAVQR + 2 Deamidated (NQ)
2757 854.9532 1707.8918 1707.8931 -0.71 1 25 0.66 +1Score > 39 indicates identity
Score > 35 indicates homology		 LQQLGVEILSTGGTYK + 2 Deamidated (NQ)
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