MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2672 556.6473 1666.9201 1666.8930 16.2 1 8 0.6 +1Score > 37 indicates identity
Score > 18 indicates homology
IFFASSDSIALEVLR
3592 741.4039 2221.1899 2221.2326 -19.2 0 8 0.72 +1Score > 36 indicates identity
Score > 19 indicates homology
AALLQLLLGANLMVMPPTQAR + Deamidated (NQ)
3722 1154.0574 2306.1002 2306.1464 -20.0 1 8 0.8 +1Score > 39 indicates identity
Score > 19 indicates homology
FQSDLKIINDCLDGLIQNAK + 2 Deamidated (NQ)
1705 405.8967 1214.6683 1214.6507 14.5 0 8 0.9 +1Score > 40 indicates identity
Score > 20 indicates homology
ALIAGNIGTVASE
2113 351.9376 1403.7213 1403.7382 -12.0 1 8 0.97 +1Score > 41 indicates identity
Score > 20 indicates homology
SSSHHIRSPAVAR
2973 919.9499 1837.8852 1837.8734 6.46 1 8 0.97 +1Score > 40 indicates identity
Score > 20 indicates homology
LENAGVNFDYLGAVNIE + Deamidated (NQ)
1980 449.2376 1344.6910 1344.6998 -6.53 0 8 1 +1Score > 41 indicates identity
Score > 20 indicates homology
TSHSSTVGSITIR
3105 641.3536 1921.0390 1921.0057 17.3 0 8 0.45 +1Score > 36 indicates identity
Score > 17 indicates homology
ALQLRPDQYVISFQSR + Deamidated (NQ)
3131 650.9979 1949.9719 1949.9694 1.27 0 8 0.91 +1Score > 40 indicates identity
Score > 20 indicates homology
ALTAGNTGLTLNIAANYGGR + 3 Deamidated (NQ)
2428 520.6256 1558.8550 1558.8467 5.29 1 8 0.81 +1Score > 38 indicates identity
Score > 19 indicates homology
NVLGQRFIADGIQK + Deamidated (NQ)
2943 906.9439 1811.8732 1811.8478 14.0 1 8 0.95 +1Score > 40 indicates identity
Score > 20 indicates homology
AGGLIYQWDSYSPRAE
1835 426.5641 1276.6705 1276.6775 -5.52 1 8 0.69 +1Score > 41 indicates identity
Score > 19 indicates homology
KLVLGYNADGAR + Deamidated (NQ)
3598 743.0620 2226.1642 2226.1685 -1.92 1 8 0.62 +1Score > 37 indicates identity
Score > 18 indicates homology
ALYPGSFDPLTLGHLDLIER
2870 591.9731 1772.8975 1772.9043 -3.87 1 8 0.72 +1Score > 40 indicates identity
Score > 19 indicates homology
LSNALSLAPAQDTETLK + 2 Deamidated (NQ)
3190 995.0169 1988.0192 1988.0023 8.52 0 8 0.72 +1Score > 39 indicates identity
Score > 19 indicates homology
LLNSNVANDLMLLDSLLE + 2 Deamidated (NQ)
559 723.3626 722.3553 722.3520 4.55 0 8 0.73 +1Score > 41 indicates identity
Score > 19 indicates homology
TITMIE + Oxidation (M)
3256 678.0061 2030.9965 2031.0234 -13.3 1 8 0.29 +1Score > 40 indicates identity
Score > 15 indicates homology
SALYSATKSAVLMIFNGLE + Deamidated (NQ); Oxidation (M)
2242 738.3785 1474.7424 1474.7344 5.46 0 8 1 +1Score > 41 indicates identity
Score > 20 indicates homology
HFVTQYEPVLIE + Deamidated (NQ)
2921 901.9963 1801.9780 1801.9747 1.87 1 8 0.35 +1Score > 37 indicates identity
Score > 15 indicates homology
MSLLEAIVLGLVQGLTE + Deamidated (NQ); Oxidation (M)
3288 682.3592 2044.0558 2044.0854 -14.5 1 8 0.96 +1Score > 39 indicates identity
Score > 20 indicates homology
LALPFGIALHHAGLTENDR
1673 600.3743 1198.7340 1198.7285 4.65 1 8 0.96 +1Score > 32 indicates identity
Score > 20 indicates homology
KAQNLALSLLK + Deamidated (NQ)
2176 717.8652 1433.7158 1433.7336 -12.4 1 8 0.72 +1Score > 41 indicates identity
Score > 19 indicates homology
WLQSSNGMRLIK + 2 Deamidated (NQ)
2513 534.2891 1599.8455 1599.8468 -0.82 0 8 0.92 +1Score > 40 indicates identity
Score > 20 indicates homology
SVQLVQLGSASALAQE
3198 500.0014 1995.9765 1996.0000 -11.8 1 8 0.92 +1Score > 40 indicates identity
Score > 20 indicates homology
LLNINGDPVAGEIASALQAE + 2 Deamidated (NQ)
3476 718.4185 2152.2337 2152.2442 -4.88 1 8 1 +1Score > 30 indicates identity
Score > 20 indicates homology
IIYVLLPNIMSISANHVKK
3551 1098.5856 2195.1566 2195.1330 10.8 1 8 0.65 +1Score > 37 indicates identity
Score > 18 indicates homology
MKSASIPIYLQQAIMNTLR + 2 Deamidated (NQ); Oxidation (M)
3785 788.3923 2362.1551 2362.1726 -7.42 1 7 0.63 +1Score > 39 indicates identity
Score > 18 indicates homology
QSILLEFVSANPTGPMNIVSAR + 3 Deamidated (NQ); Oxidation (M)
2724 566.2951 1695.8635 1695.8726 -5.40 1 7 0.69 +1Score > 40 indicates identity
Score > 18 indicates homology
AVCGKPKNPVDQVQR + Deamidated (NQ)
3342 696.0266 2085.0580 2085.0466 5.46 1 7 0.93 +1Score > 39 indicates identity
Score > 20 indicates homology
YHGLSVGVILNTMHPFER + Oxidation (M)
2345 759.9176 1517.8206 1517.8309 -6.78 0 7 0.7 +1Score > 39 indicates identity
Score > 18 indicates homology
LLKPTVMMNLLGR + Deamidated (NQ); 2 Oxidation (M)
1901 437.2233 1308.6481 1308.6271 16.0 0 7 0.73 +1Score > 41 indicates identity
Score > 19 indicates homology
DNLNQVMVVFK + 3 Deamidated (NQ)
1823 636.8405 1271.6664 1271.6622 3.34 1 7 0.92 +1Score > 41 indicates identity
Score > 20 indicates homology
RNVPIYQEPR + Deamidated (NQ)
2022 456.2300 1365.6682 1365.6598 6.10 1 7 0.66 +1Score > 41 indicates identity
Score > 18 indicates homology
VRGYMSQLGPIE + Deamidated (NQ); Oxidation (M)
1330 518.2993 1034.5840 1034.6012 -16.6 1 7 0.82 +1Score > 38 indicates identity
Score > 19 indicates homology
IEIYIVGTK
2341 506.2708 1515.7906 1515.8144 -15.7 1 7 0.96 +1Score > 41 indicates identity
Score > 20 indicates homology
DKLITVQVTIDNR + 2 Deamidated (NQ)
2007 452.9274 1355.7604 1355.7449 11.4 1 7 0.69 +1Score > 38 indicates identity
Score > 18 indicates homology
QVPKGLGAPVFDK + Deamidated (NQ)
4266 586.3076 2926.5016 2926.4560 15.6 1 7 0.67 +1Score > 36 indicates identity
Score > 18 indicates homology
EPETYGHLTLNDIVQSLSADAQALNVK + Deamidated (NQ)
3169 991.4619 1980.9092 1980.9164 -3.61 1 7 0.52 +1Score > 39 indicates identity
Score > 17 indicates homology
VQNIGKTSIDDVNVSDFE + 2 Deamidated (NQ)
2060 461.9236 1382.7490 1382.7518 -2.02 0 7 0.98 +1Score > 38 indicates identity
Score > 20 indicates homology
LAPVGSAVASVAADR
3513 726.3954 2176.1644 2176.1964 -14.7 1 7 0.81 +1Score > 36 indicates identity
Score > 19 indicates homology
QIGRNVIALDQASILTHLGR + 2 Deamidated (NQ)
3506 725.0726 2172.1960 2172.1731 10.5 0 7 0.77 +1Score > 34 indicates identity
Score > 19 indicates homology
DQGAANFLIFSLLIPFIHR + Deamidated (NQ)
4060 673.3453 2689.3521 2689.3785 -9.83 1 7 0.55 +1Score > 37 indicates identity
Score > 17 indicates homology
ALFQPNVLGLESGGIHVTTFNSIMK + Deamidated (NQ); Oxidation (M)
3094 639.3278 1914.9616 1914.9952 -17.5 1 7 0.45 +1Score > 40 indicates identity
Score > 16 indicates homology
QVLEPAFRQPYPNVTR + Deamidated (NQ)
1872 431.9087 1292.7043 1292.6823 17.0 1 7 0.69 +1Score > 38 indicates identity
Score > 18 indicates homology
IIESTSTSITNK
2255 738.9024 1475.7902 1475.7653 16.9 1 7 0.83 +1Score > 40 indicates identity
Score > 19 indicates homology
DNIKIMSALINAR + 2 Deamidated (NQ); Oxidation (M)
2526 804.5009 1606.9872 1606.9657 13.4 1 7 1 +1Score > 25 indicates identity
Score > 20 indicates homology
IQLIKNIIQPALNK + 2 Deamidated (NQ)
3562 733.3989 2197.1749 2197.1565 8.37 1 7 0.71 +1Score > 36 indicates identity
Score > 18 indicates homology
ISLDQMHKFLAQAQSPLLR + 2 Deamidated (NQ)
2120 703.8965 1405.7784 1405.7790 -0.39 0 7 0.99 +1Score > 37 indicates identity
Score > 20 indicates homology
PAARPAGAGVGLVDR
2502 533.2793 1596.8161 1596.8471 -19.4 0 7 0.67 +1Score > 40 indicates identity
Score > 18 indicates homology
IVIANSAPNNTNVLR + 2 Deamidated (NQ)
2974 613.6743 1838.0011 1837.9673 18.4 0 7 0.45 +1Score > 37 indicates identity
Score > 16 indicates homology
APVSSVASDIVAEPVAVVE
1306 514.2921 1026.5696 1026.5822 -12.2 0 7 0.67 +1Score > 39 indicates identity
Score > 18 indicates homology
LALQLGADAR
4033 879.4454 2635.3144 2635.3163 -0.72 1 7 0.51 +1Score > 38 indicates identity
Score > 17 indicates homology
ILTPLNVQQAADSRDSLAMALYAR + 3 Deamidated (NQ); Oxidation (M)
4273 734.3842 2933.5077 2933.5208 -4.48 0 7 0.39 +1Score > 36 indicates identity
Score > 16 indicates homology
VAAGYGHAPNTLTDPVVLALPGTLPVMNK + 2 Deamidated (NQ); Oxidation (M)
1599 585.8282 1169.6418 1169.6516 -8.37 1 7 0.84 +1Score > 39 indicates identity
Score > 19 indicates homology
QALRDAGIGLR + Deamidated (NQ)
2261 494.6127 1480.8163 1480.8362 -13.4 1 7 0.92 +1Score > 37 indicates identity
Score > 19 indicates homology
LTAAKHSLAGSQLGK
2928 602.6398 1804.8976 1804.9281 -16.9 0 7 0.92 +1Score > 40 indicates identity
Score > 19 indicates homology
IATIFQDPMTSLDPIK + Oxidation (M)
585 366.2275 730.4404 730.4411 -0.91 0 7 0.3 +1Score > 40 indicates identity
Score > 14 indicates homology
VAILMGK
3041 626.3055 1875.8947 1875.9070 -6.57 1 7 0.61 +1Score > 40 indicates identity
Score > 18 indicates homology
NVMMPQLIAGLTQAEAR + 2 Deamidated (NQ); 2 Oxidation (M)
3052 471.4819 1881.8985 1881.9005 -1.06 1 7 0.86 +1Score > 40 indicates identity
Score > 19 indicates homology
QLIDWMTVKPAECFK + Deamidated (NQ); Oxidation (M)
1885 434.2076 1299.6010 1299.6208 -15.2 1 7 0.9 +1Score > 40 indicates identity
Score > 19 indicates homology
QRHDTDTIWK + Deamidated (NQ)
3569 552.2933 2205.1441 2205.1212 10.4 1 7 0.93 +1Score > 38 indicates identity
Score > 19 indicates homology
VGNYRLPSNKPSGMDTALLR + Deamidated (NQ); Oxidation (M)
3873 813.7901 2438.3485 2438.3421 2.62 1 7 0.82 +1Score > 32 indicates identity
Score > 19 indicates homology
NVVLFGHSIGGGLAIALTQVLTKE + 2 Deamidated (NQ)
3260 1017.5433 2033.0720 2033.0793 -3.57 0 7 0.78 +1Score > 38 indicates identity
Score > 19 indicates homology
VSIFGATGSIGQNTIDLIAR + Deamidated (NQ)
3811 796.0928 2385.2566 2385.2362 8.53 1 7 0.52 +1Score > 37 indicates identity
Score > 17 indicates homology
MTEPVRVQVQTANPYPVIIGR + 2 Deamidated (NQ); Oxidation (M)
3220 1006.0259 2010.0372 2010.0547 -8.71 1 7 0.93 +1Score > 39 indicates identity
Score > 19 indicates homology
ADAALAASIFHFRQHSIR
3702 576.0709 2300.2545 2300.2609 -2.79 1 7 0.43 +1Score > 35 indicates identity
Score > 16 indicates homology
MSMGIAARPPRAALLPPPSVPR + Oxidation (M)
593 369.1959 736.3772 736.3755 2.35 1 7 0.77 +1Score > 42 indicates identity
Score > 18 indicates homology
ENLAYK
3055 628.6425 1882.9057 1882.9074 -0.93 1 7 0.96 +1Score > 40 indicates identity
Score > 19 indicates homology
ALAWRLDGQGPDNGAWR + Deamidated (NQ)
2179 718.3801 1434.7456 1434.7606 -10.4 1 7 0.68 +1Score > 41 indicates identity
Score > 18 indicates homology
IAPGNTLPLAEIPE + Deamidated (NQ)
2279 748.4576 1494.9006 1494.8770 15.8 0 7 0.27 +1Score > 30 indicates identity
Score > 14 indicates homology
LAADVTGVPTLLVAR
3361 699.3771 2095.1095 2095.0797 14.2 1 7 1 +1Score > 37 indicates identity
Score > 20 indicates homology
ALVAQIQQIDVTVTHTETE
2490 397.7240 1586.8669 1586.8416 15.9 1 7 0.75 +1Score > 38 indicates identity
Score > 18 indicates homology
LEPQGLANSKVQFR + Deamidated (NQ)
3233 673.7214 2018.1424 2018.1088 16.6 1 7 0.93 +1Score > 32 indicates identity
Score > 19 indicates homology
QVANKISQFLTLLWDLK + 2 Deamidated (NQ)
3568 735.3871 2203.1395 2203.1194 9.09 1 7 0.69 +1Score > 38 indicates identity
Score > 18 indicates homology
ASPAQIAAFAVAMQVKVPTSAE + Deamidated (NQ); Oxidation (M)
2020 683.3272 1364.6398 1364.6554 -11.4 0 7 0.88 +1Score > 41 indicates identity
Score > 19 indicates homology
YWLYPYGTFR
3300 685.3846 2053.1320 2053.1280 1.95 1 7 0.85 +1Score > 35 indicates identity
Score > 19 indicates homology
SQAALAVNISAARGLQDVLR + Deamidated (NQ)
3539 731.3871 2191.1395 2191.1637 -11.1 1 7 0.29 +1Score > 38 indicates identity
Score > 14 indicates homology
QQFGRIINIASVNGLVGFAGK + 3 Deamidated (NQ)
3741 773.3998 2317.1776 2317.1961 -7.99 1 7 0.41 +1Score > 38 indicates identity
Score > 16 indicates homology
NCNITVLHNLNKHITVNGQK + Deamidated (NQ)
1031 463.2258 924.4370 924.4375 -0.46 1 7 0.59 +1Score > 41 indicates identity
Score > 17 indicates homology
VIYENMR + Deamidated (NQ)
3032 623.3384 1866.9934 1866.9986 -2.78 1 7 0.92 +1Score > 38 indicates identity
Score > 19 indicates homology
IVAKGNVVTVMDHGASLR + Deamidated (NQ)
3759 1170.5742 2339.1338 2339.0991 14.8 1 7 0.46 +1Score > 39 indicates identity
Score > 16 indicates homology
YPLSFVNCVNSIGNGLVIEDE + Deamidated (NQ)
2321 505.2510 1512.7312 1512.7056 16.9 1 7 0.46 +1Score > 40 indicates identity
Score > 16 indicates homology
APEDSQSHQVSLSK + Deamidated (NQ)
2579 406.7163 1622.8361 1622.8165 12.1 1 7 0.86 +1Score > 40 indicates identity
Score > 19 indicates homology
KDNYIGGGILHHNGK + Deamidated (NQ)
985 455.3029 908.5912 908.5807 11.6 0 7 0.62 +1Score > 27 indicates identity
Score > 17 indicates homology
IGGVPVVIR
3489 722.6944 2165.0614 2165.1005 -18.1 0 7 0.58 +1Score > 39 indicates identity
Score > 17 indicates homology
VLSDVVASQAALHGPYGGVVPE + Deamidated (NQ)
696 392.7338 783.4530 783.4491 5.10 0 7 0.75 +1Score > 37 indicates identity
Score > 18 indicates homology
GPLPTTAK
2786 862.9891 1723.9636 1723.9608 1.68 0 7 0.27 +1Score > 35 indicates identity
Score > 14 indicates homology
LLVLDEPTQGIDIIGK + Deamidated (NQ)
1201 493.8204 985.6262 985.6106 15.8 1 7 0.5 +1Score > 31 indicates identity
Score > 16 indicates homology
RGLLMIVGK
1867 645.8599 1289.7052 1289.7191 -10.7 1 7 0.95 +1Score > 39 indicates identity
Score > 19 indicates homology
AISTGTNAIVSKK + Deamidated (NQ)
3886 616.0811 2460.2953 2460.2464 19.9 1 7 0.6 +1Score > 36 indicates identity
Score > 17 indicates homology
VFLLDYGNTLVVPEVWQLPQE + 2 Deamidated (NQ)
3887 821.1180 2460.3322 2460.2894 17.4 1 7 0.96 +1Score > 34 indicates identity
Score > 19 indicates homology
IQISINKSQGTLIISDNGIGMTR + 2 Deamidated (NQ)
2626 823.4490 1644.8834 1644.8756 4.77 1 7 0.58 +1Score > 39 indicates identity
Score > 17 indicates homology
LSINKLLDMAAQIAE + Oxidation (M)
2640 551.2933 1650.8581 1650.8465 7.04 0 7 0.64 +1Score > 40 indicates identity
Score > 17 indicates homology
ITTVQAAIDFIQSSR + 2 Deamidated (NQ)
2721 847.4255 1692.8364 1692.8181 10.8 0 7 0.99 +1Score > 41 indicates identity
Score > 19 indicates homology
MPFLVSVQSADHFAK + Deamidated (NQ); Oxidation (M)
3750 777.4179 2329.2319 2329.1954 15.7 1 7 1 +1Score > 36 indicates identity
Score > 19 indicates homology
TLKWQTLQDVSAPITPPYDR + Deamidated (NQ)
2123 469.9146 1406.7220 1406.7228 -0.57 1 7 0.84 +1Score > 41 indicates identity
Score > 19 indicates homology
LKVDLMSFSSHK + Oxidation (M)
2935 603.3123 1806.9151 1806.9146 0.29 1 7 0.92 +1Score > 40 indicates identity
Score > 19 indicates homology
LCSTLSLAAVSARGATTE
2390 515.2897 1542.8473 1542.8630 -10.2 1 7 0.89 +1Score > 38 indicates identity
Score > 19 indicates homology
LVSAELQHLGAHLR
2995 927.9572 1853.8998 1853.9054 -2.99 1 7 0.63 +1Score > 40 indicates identity
Score > 17 indicates homology
IVTMSHQALGDVAGRGNE
2411 777.4142 1552.8138 1552.8209 -4.55 1 7 0.98 +1Score > 39 indicates identity
Score > 19 indicates homology
VTVQGNRNNPLLVE + Deamidated (NQ)
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