MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2785 862.4788 1722.9430 1722.9192 13.8 1 8 0.61 +1Score > 37 indicates identity
Score > 19 indicates homology
LQSNTKIVLFSGWTK + 2 Deamidated (NQ)
2224 366.4664 1461.8365 1461.8079 19.6 1 8 0.62 +1Score > 35 indicates identity
Score > 19 indicates homology
TTEGLAGALFQLIK + Deamidated (NQ)
2691 558.3119 1671.9139 1671.9235 -5.78 1 8 0.26 +1Score > 38 indicates identity
Score > 15 indicates homology
YVAYNAYKLIVLNK + Deamidated (NQ)
3594 1112.0237 2222.0328 2222.0678 -15.7 1 8 0.77 +1Score > 38 indicates identity
Score > 20 indicates homology
QILPLFDQFTQDIAAECGR + Deamidated (NQ)
2468 527.6370 1579.8892 1579.8835 3.61 1 8 0.5 +1Score > 35 indicates identity
Score > 18 indicates homology
GLPVTIFPDPRVNR
3088 638.3117 1911.9133 1911.9473 -17.8 0 8 0.97 +1Score > 40 indicates identity
Score > 21 indicates homology
HMDQLQQNVSIVTQQK + Oxidation (M)
3395 706.0325 2115.0757 2115.1133 -17.8 0 8 0.8 +1Score > 39 indicates identity
Score > 20 indicates homology
TVTVLNTILLQNMGAALTPE + Deamidated (NQ); Oxidation (M)
3901 826.4407 2476.3003 2476.2996 0.29 1 8 0.4 +1Score > 36 indicates identity
Score > 17 indicates homology
VPTLDGHVKLTIPAGTQPNTMLR + 2 Deamidated (NQ); Oxidation (M)
2293 500.6704 1498.9894 1498.9824 4.68 1 8 0.15 +1Score > 13 indicates identity GLLPVLRPLPRLR
2486 529.6292 1585.8658 1585.8940 -17.8 1 8 0.54 +1Score > 38 indicates identity
Score > 18 indicates homology
RLSGNLSIPNVFIR + Deamidated (NQ)
2711 843.9465 1685.8784 1685.8736 2.86 1 8 0.89 +1Score > 40 indicates identity
Score > 20 indicates homology
ATWAKNIQAAINQVR + 3 Deamidated (NQ)
3189 994.9768 1987.9390 1987.9375 0.79 1 8 0.81 +1Score > 39 indicates identity
Score > 20 indicates homology
SLVTSFSDFSDVTQGELR + Deamidated (NQ)
2546 806.9557 1611.8968 1611.8944 1.51 1 8 0.47 +1Score > 37 indicates identity
Score > 17 indicates homology
TKTIIDQAGATPGAIR
3939 837.4714 2509.3924 2509.4011 -3.49 1 8 0.31 +1Score > 32 indicates identity
Score > 16 indicates homology
LAQLILSMPNLNLRGIMAVPSIK + 2 Deamidated (NQ); Oxidation (M)
4388 778.9271 3111.6793 3111.6454 10.9 0 8 1 +1Score > 32 indicates identity
Score > 21 indicates homology
IVSSLVNNIIMPFVGVLLGGLDFSDLSFK + 2 Deamidated (NQ); Oxidation (M)
1376 354.5301 1060.5685 1060.5513 16.2 0 8 0.89 +1Score > 41 indicates identity
Score > 20 indicates homology
SNLATSAQIR + Deamidated (NQ)
1425 362.5464 1084.6174 1084.6240 -6.13 1 8 0.63 +1Score > 39 indicates identity
Score > 19 indicates homology
LGRLAQQGLK + 2 Deamidated (NQ)
3336 520.5438 2078.1461 2078.1081 18.3 1 8 0.24 +1Score > 34 indicates identity
Score > 15 indicates homology
QMLAGLLKSLGGTLNFLQR + 3 Deamidated (NQ); Oxidation (M)
3377 703.0198 2106.0376 2106.0224 7.18 1 8 0.44 +1Score > 39 indicates identity
Score > 17 indicates homology
MLGLDSMADAQELSIIGVVE + Oxidation (M)
1962 668.8314 1335.6482 1335.6340 10.7 1 8 0.79 +1Score > 41 indicates identity
Score > 20 indicates homology
MNELDSLVATAR + Deamidated (NQ); Oxidation (M)
1343 522.2761 1042.5376 1042.5519 -13.7 1 8 0.65 +1Score > 41 indicates identity
Score > 19 indicates homology
ADGASTPRLR
3725 578.0345 2308.1089 2308.0933 6.75 1 8 0.23 +1Score > 39 indicates identity
Score > 14 indicates homology
FIILSDILGDEDHLGDMDFK + Oxidation (M)
2960 913.4375 1824.8604 1824.8671 -3.66 1 8 0.26 +1Score > 40 indicates identity
Score > 15 indicates homology
VMAALCLATDLMDLKE + 2 Oxidation (M)
3085 636.7192 1907.1358 1907.0979 19.9 1 8 1 +1Score > 27 indicates identity
Score > 21 indicates homology
LPVLGAVIKNNILDVVTE + Deamidated (NQ)
4030 659.8149 2635.2305 2635.2186 4.50 1 8 0.25 +1Score > 37 indicates identity
Score > 15 indicates homology
GQTVFMGDFPLMTDKGTFIINGTE + Deamidated (NQ); Oxidation (M)
2311 753.9341 1505.8536 1505.8314 14.8 1 8 0.53 +1Score > 35 indicates identity
Score > 18 indicates homology
LLEPSLGKSGVNHR
2699 837.4801 1672.9456 1672.9359 5.81 1 8 0.54 +1Score > 35 indicates identity
Score > 18 indicates homology
LVQSQLLKSVVSGSAR + 2 Deamidated (NQ)
465 342.2269 682.4392 682.4378 2.18 0 8 0.84 +1Score > 34 indicates identity
Score > 20 indicates homology
IAVVPGK
1146 481.7762 961.5378 961.5379 -0.025 1 8 0.85 +1Score > 39 indicates identity
Score > 20 indicates homology
VGAMTSLRK
3019 622.3085 1863.9037 1863.9400 -19.5 1 8 0.68 +1Score > 40 indicates identity
Score > 19 indicates homology
SVKGQMLQDPFLNTLR + 2 Deamidated (NQ); Oxidation (M)
3020 622.3098 1863.9076 1863.8851 12.1 1 8 0.64 +1Score > 40 indicates identity
Score > 19 indicates homology
AFVSIEDNVVAGGAGSGVSE
3122 648.0318 1941.0736 1941.0611 6.43 0 8 0.65 +1Score > 35 indicates identity
Score > 19 indicates homology
LIIDWHNLAYSILQLK + 2 Deamidated (NQ)
3481 719.7215 2156.1427 2156.1338 4.11 1 8 0.46 +1Score > 37 indicates identity
Score > 17 indicates homology
VSNRDAAQLVASDLGFALGPR
2025 456.2312 1365.6718 1365.6637 5.94 1 8 0.97 +1Score > 41 indicates identity
Score > 21 indicates homology
RSHDALVASNPAE
3007 620.3216 1857.9430 1857.9486 -3.01 1 8 0.57 +1Score > 40 indicates identity
Score > 18 indicates homology
YRYNHLDLGDIGGIPR
2305 752.4182 1502.8218 1502.8456 -15.8 1 8 0.99 +1Score > 38 indicates identity
Score > 21 indicates homology
IANVNKALDFIASK
3065 630.3453 1888.0141 1887.9942 10.5 1 8 0.49 +1Score > 38 indicates identity
Score > 18 indicates homology
FGRLVQLQTLILQDNE + 2 Deamidated (NQ)
3447 714.7215 2141.1427 2141.1820 -18.3 1 8 0.82 +1Score > 37 indicates identity
Score > 20 indicates homology
RGADTIVVVPLLFAAGMHFK
3755 585.0388 2336.1261 2336.1179 3.51 1 8 0.54 +1Score > 39 indicates identity
Score > 18 indicates homology
NASKGPHSPQVPSQVQSPMTSR + Deamidated (NQ); Oxidation (M)
3221 671.0385 2010.0937 2010.0898 1.91 1 8 0.38 +1Score > 36 indicates identity
Score > 16 indicates homology
LASIKGLHDIGTFVAPDTR
1812 633.8768 1265.7390 1265.7496 -8.33 1 8 0.19 +1Score > 34 indicates identity
Score > 13 indicates homology
VPLELVPIIHH
2147 474.8929 1421.6569 1421.6834 -18.6 0 8 0.88 +1Score > 41 indicates identity
Score > 20 indicates homology
MVDGHAQTIAHAR + Oxidation (M)
3185 663.3480 1987.0222 1987.0098 6.24 1 8 0.74 +1Score > 39 indicates identity
Score > 19 indicates homology
LAMLSTHYRQPINWTR + Deamidated (NQ)
1699 607.3349 1212.6552 1212.6615 -5.15 0 8 0.29 +1Score > 40 indicates identity
Score > 15 indicates homology
VFSNDIHLLR
2934 603.3113 1806.9121 1806.9251 -7.22 1 8 0.37 +1Score > 40 indicates identity
Score > 16 indicates homology
SIGVSNFTEDLLATVIE
3515 1090.0338 2178.0530 2178.0853 -14.8 0 8 0.83 +1Score > 39 indicates identity
Score > 20 indicates homology
LMLASMFHSLFAIGSQLSPE
2225 732.3940 1462.7734 1462.7901 -11.4 1 8 0.91 +1Score > 40 indicates identity
Score > 20 indicates homology
KTHMISMHPIIR
3882 816.7599 2447.2579 2447.2471 4.39 1 8 0.36 +1Score > 38 indicates identity
Score > 16 indicates homology
IKWGQYVTQNQILGINDVLIE + 4 Deamidated (NQ)
533 354.7071 707.3996 707.3966 4.32 1 8 0.85 +1Score > 37 indicates identity
Score > 20 indicates homology
KYAGAAK
1541 380.5693 1138.6861 1138.6822 3.38 0 8 0.7 +1Score > 33 indicates identity
Score > 19 indicates homology
LRPLIADSVR
3537 730.3839 2188.1299 2188.1636 -15.4 0 8 0.78 +1Score > 38 indicates identity
Score > 19 indicates homology
MFTIDIILQMSPIPVSVQR + Deamidated (NQ)
3986 856.4779 2566.4119 2566.3837 11.0 1 8 0.29 +1Score > 33 indicates identity
Score > 15 indicates homology
MNKPFLILLIALIAFSGCNMRK + Deamidated (NQ); Oxidation (M)
3034 623.6557 1867.9453 1867.9136 16.9 1 8 0.72 +1Score > 39 indicates identity
Score > 19 indicates homology
VDIRQHGSGNAGATNTLR + 2 Deamidated (NQ)
3758 780.4009 2338.1809 2338.1474 14.3 0 8 0.78 +1Score > 38 indicates identity
Score > 19 indicates homology
SVHANILNDISPQIVSDMINR + 3 Deamidated (NQ)
4539 840.9642 3359.8277 3359.8931 -19.5 1 8 0.3 +1Score > 31 indicates identity
Score > 15 indicates homology
YDQLLSFAWKVALPLSLLNLMLTGIVVVAR + Deamidated (NQ); Oxidation (M)
2780 860.4635 1718.9124 1718.9138 -0.76 1 8 0.89 +1Score > 39 indicates identity
Score > 20 indicates homology
GMGNLLDFQLRLAQK + Oxidation (M)
2981 461.4889 1841.9265 1841.9537 -14.7 1 8 0.8 +1Score > 40 indicates identity
Score > 20 indicates homology
QVQFAHAATQHYKSVK
3347 696.6768 2087.0086 2086.9816 12.9 1 8 0.99 +1Score > 39 indicates identity
Score > 20 indicates homology
YSLLTNSWSTAKSMNMPR + Deamidated (NQ)
1523 378.5259 1132.5559 1132.5360 17.5 1 8 0.88 +1Score > 42 indicates identity
Score > 20 indicates homology
KVLGNDSDQR + 2 Deamidated (NQ)
1535 379.8913 1136.6521 1136.6441 7.01 0 8 0.91 +1Score > 36 indicates identity
Score > 20 indicates homology
SIASTIASIFK
3561 733.3976 2197.1710 2197.1670 1.79 1 8 0.18 +1Score > 37 indicates identity
Score > 13 indicates homology
FAYASTFKALLAGAVLQSLPE + Deamidated (NQ)
1650 594.8245 1187.6344 1187.6146 16.7 1 8 0.79 +1Score > 41 indicates identity
Score > 19 indicates homology
RVAQQLLSGSE + Deamidated (NQ)
514 351.7325 701.4504 701.4476 4.06 0 8 0.46 +1Score > 35 indicates identity
Score > 17 indicates homology
VPIFVK
554 361.2171 720.4196 720.4204 -1.04 1 8 0.98 +1Score > 38 indicates identity
Score > 20 indicates homology
MTKVVK + Oxidation (M)
3461 716.4178 2146.2316 2146.1898 19.5 1 8 1 +1Score > 30 indicates identity
Score > 20 indicates homology
ILGHLDLLNAKAQYAHQLK + Deamidated (NQ)
2595 816.4543 1630.8940 1630.8719 13.6 0 8 1 +1Score > 37 indicates identity
Score > 20 indicates homology
LTLHGLQQYFVALE
2708 843.4814 1684.9482 1684.9247 14.0 1 8 0.82 +1Score > 36 indicates identity
Score > 20 indicates homology
QAQILLESVNIITNK + 2 Deamidated (NQ)
3419 1064.5632 2127.1118 2127.0823 13.9 1 8 0.57 +1Score > 38 indicates identity
Score > 18 indicates homology
AGRYQFMVYAGPLSEPTLK
2031 456.5703 1366.6891 1366.7133 -17.7 0 8 0.72 +1Score > 40 indicates identity
Score > 19 indicates homology
ATFTTQVLNPFK + Deamidated (NQ)
2110 701.8998 1401.7850 1401.8014 -11.6 1 8 0.84 +1Score > 37 indicates identity
Score > 20 indicates homology
TMLSRLVISPTGK
2599 408.7471 1630.9593 1630.9406 11.4 1 8 0.86 +1Score > 31 indicates identity
Score > 20 indicates homology
LDTVLFQKQLGTLR
2664 556.6258 1666.8556 1666.8301 15.3 1 8 0.61 +1Score > 40 indicates identity
Score > 18 indicates homology
ITATELAGLADTISYE
3135 653.0301 1956.0685 1956.0424 13.3 1 8 0.95 +1Score > 36 indicates identity
Score > 20 indicates homology
MLTLAAELGADVPVCLVGK
1890 435.2614 1302.7624 1302.7395 17.6 0 8 0.55 +1Score > 34 indicates identity
Score > 18 indicates homology
IVLVVNSGSSSIK + Deamidated (NQ)
2283 749.9359 1497.8572 1497.8402 11.4 0 8 0.89 +1Score > 35 indicates identity
Score > 20 indicates homology
LSLDGALDQAAALLK
225 506.2674 505.2601 505.2570 6.15 0 8 1 +1Score > 43 indicates identity
Score > 20 indicates homology
MGGLL + Oxidation (M)
1374 530.7458 1059.4770 1059.4945 -16.5 0 8 0.77 +1Score > 39 indicates identity
Score > 19 indicates homology
QATDNGIQGR + Deamidated (NQ)
2102 467.6089 1399.8049 1399.7935 8.09 1 8 0.96 +1Score > 36 indicates identity
Score > 20 indicates homology
LAGNPLLRYDIR
3789 789.4026 2365.1860 2365.1922 -2.65 1 8 0.63 +1Score > 38 indicates identity
Score > 18 indicates homology
NNTPYLGICLGMQVAVIEFAR
472 343.6675 685.3204 685.3143 8.93 0 8 0.58 +1Score > 37 indicates identity
Score > 18 indicates homology
NPNGQR + Deamidated (NQ)
2450 524.9781 1571.9125 1571.9148 -1.46 1 8 0.21 +1Score > 33 indicates identity
Score > 14 indicates homology
VVRVQPPAPPVAVSR + Deamidated (NQ)
2897 895.9016 1789.7886 1789.7936 -2.77 0 8 0.42 +1Score > 38 indicates identity
Score > 17 indicates homology
ASQSVMQFMQNMLIK + 3 Deamidated (NQ); 2 Oxidation (M)
3682 1147.5847 2293.1548 2293.1240 13.5 1 8 0.89 +1Score > 39 indicates identity
Score > 20 indicates homology
FWLGNDHIHALTAQGTNELR + Deamidated (NQ)
3223 671.3330 2010.9772 2010.9979 -10.3 1 8 0.72 +1Score > 40 indicates identity
Score > 19 indicates homology
MAIAQNVGAMSSYLNRLR + Deamidated (NQ); Oxidation (M)
1613 587.2880 1172.5614 1172.5713 -8.43 0 8 0.84 +1Score > 40 indicates identity
Score > 20 indicates homology
LQLLQQDWE + Deamidated (NQ)
2565 808.4554 1614.8962 1614.8916 2.88 1 8 0.85 +1Score > 37 indicates identity
Score > 20 indicates homology
VGGMGDVVGALPKFLR
4514 662.3714 3306.8206 3306.8380 -5.25 1 8 0.83 +1Score > 30 indicates identity
Score > 20 indicates homology
NFLLLLLAAYALGSTPFAIVSSRLFGLADPR + Deamidated (NQ)
2447 524.2921 1569.8545 1569.8838 -18.7 1 8 0.96 +1Score > 39 indicates identity
Score > 20 indicates homology
AIIASGVQVQKSDVR
2704 562.2942 1683.8608 1683.8356 15.0 1 8 0.81 +1Score > 40 indicates identity
Score > 19 indicates homology
NGQVFITNLATFTEK + 2 Deamidated (NQ)
2623 548.9676 1643.8810 1643.8743 4.04 1 8 0.62 +1Score > 39 indicates identity
Score > 18 indicates homology
QLGPQAQYAGRSVLR + Deamidated (NQ)
2731 566.9874 1697.9404 1697.9440 -2.11 0 8 0.62 +1Score > 36 indicates identity
Score > 18 indicates homology
VPRPLFPLPMFNVR + Oxidation (M)
3246 1012.5308 2023.0470 2023.0408 3.07 0 8 0.46 +1Score > 39 indicates identity
Score > 17 indicates homology
VTALADGVLSTGQMVTYAAR
3655 1134.0789 2266.1432 2266.1805 -16.4 1 8 0.28 +1Score > 39 indicates identity
Score > 15 indicates homology
NPIQDIKITSDTNVPVNAGIR + 2 Deamidated (NQ)
3677 761.4078 2281.2016 2281.1703 13.7 1 8 0.22 +1Score > 37 indicates identity
Score > 14 indicates homology
FIVGNTAPVNGDSYKSGTVTVR
3911 830.4592 2488.3558 2488.3397 6.44 1 8 0.26 +1Score > 34 indicates identity
Score > 14 indicates homology
VDSLIKSLIPNNIHNLQVSNNR + Deamidated (NQ)
526 354.1787 706.3428 706.3432 -0.50 0 8 0.82 +1Score > 41 indicates identity
Score > 19 indicates homology
AGMVSSR
1353 1047.5441 1046.5368 1046.5430 -5.90 1 8 0.79 +1Score > 42 indicates identity
Score > 19 indicates homology
QPQISKMAK + Deamidated (NQ); Oxidation (M)
3162 990.5215 1979.0284 1979.0372 -4.45 1 8 0.92 +1Score > 39 indicates identity
Score > 20 indicates homology
FLKCLGPNLIFDMGLNK
1228 498.7806 995.5466 995.5400 6.68 0 8 0.69 +1Score > 38 indicates identity
Score > 19 indicates homology
APPTTAVPSR
1420 542.7633 1083.5120 1083.5196 -6.99 1 8 0.85 +1Score > 40 indicates identity
Score > 19 indicates homology
NKSPPQNAVE + Deamidated (NQ)
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