MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2136 707.9132 1413.8118 1413.8132 -0.98 1 9 0.47 +1Score > 36 indicates identity
Score > 18 indicates homology
LLFLIPPEHAHK
1608 391.5693 1171.6861 1171.6673 16.0 1 9 0.82 +1Score > 37 indicates identity
Score > 21 indicates homology
ANTGGLSKLVGR
2055 460.5609 1378.6609 1378.6616 -0.54 1 9 0.63 +1Score > 41 indicates identity
Score > 20 indicates homology
SGVVSPFVETNNK + 2 Deamidated (NQ)
2171 715.8724 1429.7302 1429.7048 17.8 1 9 0.8 +1Score > 41 indicates identity
Score > 21 indicates homology
QQQITDALENIR + 2 Deamidated (NQ)
2706 562.2996 1683.8770 1683.8726 2.58 1 9 0.67 +1Score > 39 indicates identity
Score > 20 indicates homology
AKNVLILMSDTGGGHR + Oxidation (M)
3014 930.9901 1859.9656 1859.9741 -4.53 1 9 0.67 +1Score > 39 indicates identity
Score > 20 indicates homology
RILNEPTAASLAYGLDR + Deamidated (NQ)
3440 1068.6113 2135.2080 2135.1739 16.0 1 9 0.47 +1Score > 31 indicates identity
Score > 18 indicates homology
WLPSLNPLDINVVVTGKDR
2792 864.4776 1726.9406 1726.9326 4.68 1 9 0.7 +1Score > 37 indicates identity
Score > 20 indicates homology
GDSVISNLQRISKPGR + Deamidated (NQ)
753 813.4369 812.4296 812.4392 -11.8 1 9 0.46 +1Score > 39 indicates identity
Score > 18 indicates homology
NPVVEVR + Deamidated (NQ)
1653 596.3038 1190.5930 1190.5965 -2.91 1 9 0.77 +1Score > 41 indicates identity
Score > 20 indicates homology
GQIDIELTMR + Oxidation (M)
2842 587.9513 1760.8321 1760.8556 -13.4 1 9 0.46 +1Score > 40 indicates identity
Score > 18 indicates homology
KGITVLFYDQFGCGR + Deamidated (NQ)
3114 967.5024 1932.9902 1932.9687 11.1 1 9 0.56 +1Score > 39 indicates identity
Score > 19 indicates homology
AINAGGGAVTVLTRDQMSR + Deamidated (NQ); Oxidation (M)
3139 653.3526 1957.0360 1957.0051 15.8 1 9 0.15 +1Score > 38 indicates identity
Score > 13 indicates homology
LNDVTISTCHVSAKVGTR
3627 562.0261 2244.0753 2244.0343 18.2 0 9 0.69 +1Score > 39 indicates identity
Score > 20 indicates homology
DAYYIHGQCAIIMFDVTAR + Deamidated (NQ)
576 365.1998 728.3850 728.3817 4.62 1 9 0.87 +1Score > 37 indicates identity
Score > 21 indicates homology
KGEPNGK
3560 550.2775 2197.0809 2197.0837 -1.29 0 9 0.76 +1Score > 39 indicates identity
Score > 20 indicates homology
ALINHLANTDDQFIMAPGVR + 2 Deamidated (NQ)
4089 678.6066 2710.3973 2710.3564 15.1 0 9 0.45 +1Score > 36 indicates identity
Score > 18 indicates homology
SANLMLSLTVLPNVNSTWYFDVPK + 2 Deamidated (NQ)
2145 711.8333 1421.6520 1421.6643 -8.60 0 9 0.77 +1Score > 40 indicates identity
Score > 20 indicates homology
IVLNMMNQDGVR + Deamidated (NQ); 2 Oxidation (M)
2958 365.7984 1823.9556 1823.9460 5.27 1 9 0.44 +1Score > 39 indicates identity
Score > 18 indicates homology
FMKVDVLGLGMLGCLR + Oxidation (M)
3889 822.1157 2463.3253 2463.3009 9.89 0 9 0.4 +1Score > 35 indicates identity
Score > 17 indicates homology
IAVAHYAAQLSTLDINPQPLSLE
3156 657.9995 1970.9767 1971.0095 -16.6 1 9 0.82 +1Score > 40 indicates identity
Score > 21 indicates homology
LSTQLIDMIHTAAARANK + 2 Deamidated (NQ); Oxidation (M)
3366 700.3732 2098.0978 2098.1166 -8.98 1 9 0.21 +1Score > 38 indicates identity
Score > 15 indicates homology
LGILPALEIVMGMDDLQVR + Oxidation (M)
2838 587.2930 1758.8572 1758.8424 8.40 1 9 0.66 +1Score > 40 indicates identity
Score > 20 indicates homology
IFPNQNQQIEQIQR + 4 Deamidated (NQ)
1808 422.5656 1264.6750 1264.6816 -5.21 0 9 0.88 +1Score > 39 indicates identity
Score > 21 indicates homology
YIFGAQVAPATK
1809 633.8355 1265.6564 1265.6768 -16.1 1 9 0.46 +1Score > 40 indicates identity
Score > 18 indicates homology
VDYVRVYQPK
3293 1023.5352 2045.0558 2045.0152 19.9 1 9 0.81 +1Score > 39 indicates identity
Score > 21 indicates homology
RYAQLGWVMQLHIGAQR + 3 Deamidated (NQ); Oxidation (M)
2655 832.4409 1662.8672 1662.8940 -16.1 1 9 0.89 +1Score > 40 indicates identity
Score > 21 indicates homology
LAQKYNTISQLLDR + Deamidated (NQ)
3323 692.7157 2075.1253 2075.0899 17.1 1 9 0.17 +1Score > 36 indicates identity
Score > 14 indicates homology
ATDLDVFLKGGAALDINSVR + Deamidated (NQ)
4184 939.1854 2814.5344 2814.5396 -1.86 1 9 1 +1Score > 32 indicates identity
Score > 21 indicates homology
MRTLGAMAIMLVVMGTVIFLSFILR + 2 Oxidation (M)
1517 566.2918 1130.5690 1130.5819 -11.4 1 9 0.85 +1Score > 41 indicates identity
Score > 21 indicates homology
NIATIDVEQK + Deamidated (NQ)
3554 1099.5409 2197.0672 2197.0474 9.05 1 9 0.94 +1Score > 39 indicates identity
Score > 21 indicates homology
VDTEGVPFTSNVAANINYMR
2268 746.3837 1490.7528 1490.7439 6.01 1 9 0.55 +1Score > 41 indicates identity
Score > 19 indicates homology
LGEQMSLFPLSPR + Deamidated (NQ); Oxidation (M)
3650 754.3927 2260.1563 2260.1409 6.78 1 9 1 +1Score > 39 indicates identity
Score > 21 indicates homology
IFTYSKLGTIAGSMQDTGVVR + Deamidated (NQ); Oxidation (M)
2170 715.8716 1429.7286 1429.7412 -8.79 1 9 0.9 +1Score > 41 indicates identity
Score > 21 indicates homology
ANSINSKLNILNE + Deamidated (NQ)
2211 729.9164 1457.8182 1457.8354 -11.8 1 9 0.41 +1Score > 37 indicates identity
Score > 17 indicates homology
TTLTRLLADIWR
3351 697.7359 2090.1859 2090.1623 11.3 1 9 1 +1Score > 31 indicates identity
Score > 21 indicates homology
YIVNTVLDLGGQTTKILDK
3828 800.0878 2397.2416 2397.2540 -5.18 1 9 0.4 +1Score > 37 indicates identity
Score > 17 indicates homology
WVRAQASVVVLEPGTIQTSVQE + Deamidated (NQ)
2882 594.6409 1780.9009 1780.9182 -9.71 1 9 0.58 +1Score > 40 indicates identity
Score > 19 indicates homology
QKPGLYDMRDILGFK + Deamidated (NQ)
2924 902.4736 1802.9326 1802.9084 13.5 0 9 0.96 +1Score > 40 indicates identity
Score > 21 indicates homology
MNTLINVTQSLINPSR + 3 Deamidated (NQ)
2996 927.9586 1853.9026 1853.9384 -19.3 0 9 0.91 +1Score > 40 indicates identity
Score > 21 indicates homology
VPAPYGTGPAAAAQAASAAGR
2374 767.4437 1532.8728 1532.8636 6.02 1 9 0.75 +1Score > 35 indicates identity
Score > 20 indicates homology
MLVIQPYDKSVIK
3618 1122.1149 2242.2152 2242.1766 17.2 1 9 0.62 +1Score > 35 indicates identity
Score > 19 indicates homology
SVSKIIANGINIALQPNMVLE + 3 Deamidated (NQ); Oxidation (M)
1864 430.5886 1288.7440 1288.7391 3.80 0 9 0.52 +1Score > 35 indicates identity
Score > 18 indicates homology
TFSIIKPDAVAK
3097 480.2450 1916.9509 1916.9150 18.7 0 9 0.83 +1Score > 40 indicates identity
Score > 20 indicates homology
LGQGVQDMIGQDQTVIGR + 3 Deamidated (NQ)
2642 826.4418 1650.8690 1650.8577 6.88 0 9 0.59 +1Score > 39 indicates identity
Score > 19 indicates homology
ILGDSGVNAVVQPEPR + Deamidated (NQ)
2154 712.8276 1423.6406 1423.6368 2.73 1 9 0.52 +1Score > 40 indicates identity
Score > 18 indicates homology
NNQSNYWEALGK + Deamidated (NQ)
1857 429.5511 1285.6315 1285.6336 -1.68 0 9 0.71 +1Score > 40 indicates identity
Score > 20 indicates homology
GTVILDMAHGQK + Deamidated (NQ); Oxidation (M)
2927 602.6392 1804.8958 1804.8625 18.4 1 9 0.72 +1Score > 40 indicates identity
Score > 20 indicates homology
TMTAAVTNVGHAVSSSKE + Oxidation (M)
2970 919.5043 1836.9940 1836.9873 3.67 1 9 0.69 +1Score > 37 indicates identity
Score > 20 indicates homology
LAVLSPTLSPVGYYTKE
2661 417.4847 1665.9097 1665.9162 -3.90 1 9 0.59 +1Score > 37 indicates identity
Score > 19 indicates homology
ANGPLRLLVVGGSQGAR + 2 Deamidated (NQ)
1439 365.2100 1092.6082 1092.5928 14.1 1 9 0.79 +1Score > 38 indicates identity
Score > 20 indicates homology
RVGAVDVSYK
2140 472.9410 1415.8012 1415.8236 -15.8 0 9 0.83 +1Score > 37 indicates identity
Score > 20 indicates homology
TVTGTLGTIVDIVK
725 399.2321 796.4496 796.4443 6.71 0 9 0.53 +1Score > 36 indicates identity
Score > 18 indicates homology
AQTPVPGK
2485 793.9375 1585.8604 1585.8788 -11.5 1 9 0.86 +1Score > 38 indicates identity
Score > 21 indicates homology
SRTQIVQNLLVGTR + 2 Deamidated (NQ)
2552 538.6531 1612.9375 1612.9413 -2.37 1 9 0.85 +1Score > 32 indicates identity
Score > 21 indicates homology
TLNLLRTLGFPVNR
2799 865.4777 1728.9408 1728.9523 -6.62 1 9 0.4 +1Score > 38 indicates identity
Score > 17 indicates homology
GAGVPFRVLGTVGGDSLK
1298 512.2610 1022.5074 1022.4920 15.1 1 9 1 +1Score > 41 indicates identity
Score > 21 indicates homology
AISEVFNNK + 2 Deamidated (NQ)
2947 907.4985 1812.9824 1813.0059 -12.9 0 9 0.73 +1Score > 37 indicates identity
Score > 20 indicates homology
IVNMIPAYVPQVLINK + 2 Deamidated (NQ)
3144 654.7117 1961.1133 1961.1424 -14.8 1 9 0.29 +1Score > 32 indicates identity
Score > 16 indicates homology
GFPKTGLLLNLLGVIFMK + Deamidated (NQ)
3455 716.0560 2145.1462 2145.1099 16.9 0 9 0.7 +1Score > 36 indicates identity
Score > 20 indicates homology
INLDNLGLLTMQANITGTSR + Deamidated (NQ)
956 449.2820 896.5494 896.5331 18.2 1 9 0.61 +1Score > 34 indicates identity
Score > 19 indicates homology
AGIPIVEAK
1377 531.2972 1060.5798 1060.5699 9.39 1 9 0.76 +1Score > 41 indicates identity
Score > 20 indicates homology
KINAVVNMR + Deamidated (NQ); Oxidation (M)
3134 651.3322 1950.9748 1950.9575 8.87 1 9 0.83 +1Score > 40 indicates identity
Score > 20 indicates homology
VLADIQQGRFALDFVQE + 3 Deamidated (NQ)
3343 1043.5375 2085.0604 2085.0466 6.65 1 9 0.87 +1Score > 39 indicates identity
Score > 21 indicates homology
YHGLSVGVILNTMHPFER + Oxidation (M)
3286 681.6895 2042.0467 2042.0354 5.52 1 9 0.54 +1Score > 39 indicates identity
Score > 18 indicates homology
NAVAVGGTNIPPTITELCSK + Deamidated (NQ)
3015 620.9966 1859.9680 1859.9736 -3.05 1 9 0.62 +1Score > 39 indicates identity
Score > 19 indicates homology
IVLQPDETIQISMMVK + Oxidation (M)
1458 552.8296 1103.6446 1103.6299 13.4 1 9 0.7 +1Score > 37 indicates identity
Score > 20 indicates homology
QVSSTVSKLR
1994 451.6171 1351.8295 1351.8438 -10.6 0 9 0.64 +1Score > 29 indicates identity
Score > 19 indicates homology
DLLAQLLALILR + Deamidated (NQ)
2736 567.6299 1699.8679 1699.8794 -6.80 1 9 0.43 +1Score > 40 indicates identity
Score > 17 indicates homology
WLSAGTKYHINPTGR
3439 1068.5658 2135.1170 2135.0858 14.6 1 9 0.38 +1Score > 38 indicates identity
Score > 17 indicates homology
ILLSLDNQDLEINQVNHR + 2 Deamidated (NQ)
3509 725.3687 2173.0843 2173.0936 -4.28 1 9 0.56 +1Score > 39 indicates identity
Score > 19 indicates homology
QLTQKKPQISIINNLCNR + 6 Deamidated (NQ)
3523 728.0605 2181.1597 2181.1463 6.12 1 9 0.4 +1Score > 37 indicates identity
Score > 17 indicates homology
VGSLAVGMQADLALLNLPDER
1700 607.3367 1212.6588 1212.6462 10.4 1 9 0.41 +1Score > 40 indicates identity
Score > 17 indicates homology
KPRTAPLGSTGE
3532 730.3735 2188.0987 2188.1173 -8.50 1 9 0.32 +1Score > 39 indicates identity
Score > 16 indicates homology
ICNGRYFINTAMILFLNK + Deamidated (NQ)
3319 692.3537 2074.0393 2074.0153 11.5 1 9 0.87 +1Score > 39 indicates identity
Score > 20 indicates homology
VAAVLQGKHDNYDIDLMR + Deamidated (NQ); Oxidation (M)
2675 556.9924 1667.9554 1667.9431 7.38 1 9 0.84 +1Score > 34 indicates identity
Score > 20 indicates homology
LRSINAQQTQQLLR
2343 759.4370 1516.8594 1516.8362 15.3 1 9 0.85 +1Score > 35 indicates identity
Score > 20 indicates homology
LGDLYRQLADVVR
2306 752.4204 1502.8262 1502.8279 -1.09 1 9 0.23 +1Score > 38 indicates identity
Score > 15 indicates homology
AYEPLIRIICQK
1830 425.5524 1273.6354 1273.6449 -7.45 0 9 0.8 +1Score > 41 indicates identity
Score > 20 indicates homology
GCISVTSNIAPR
2389 515.2894 1542.8464 1542.8413 3.31 1 9 0.65 +1Score > 38 indicates identity
Score > 19 indicates homology
QTSVTRAMRPAVAR
4083 902.4838 2704.4296 2704.3854 16.3 1 9 0.46 +1Score > 35 indicates identity
Score > 18 indicates homology
STKIYVGNVPIGGDAPIAVQSMTNTR + Oxidation (M)
553 361.2171 720.4196 720.4283 -11.9 1 8 0.99 +1Score > 38 indicates identity
Score > 21 indicates homology
VVYGKR
2016 455.2477 1362.7213 1362.7143 5.12 1 8 0.81 +1Score > 40 indicates identity
Score > 20 indicates homology
SKTNSIFQLAPR + 2 Deamidated (NQ)
1130 319.8391 956.4955 956.4927 2.89 0 8 0.7 +1Score > 39 indicates identity
Score > 19 indicates homology
DPTALDVAR
2301 752.3918 1502.7690 1502.7437 16.8 1 8 0.89 +1Score > 41 indicates identity
Score > 20 indicates homology
LTSDGAGISGGQRQR + Deamidated (NQ)
3146 655.3455 1963.0147 1963.0383 -12.0 1 8 0.85 +1Score > 39 indicates identity
Score > 20 indicates homology
MNILVLNGINLNMFGKR + Deamidated (NQ); Oxidation (M)
3510 725.3710 2173.0912 2173.1089 -8.16 1 8 0.62 +1Score > 39 indicates identity
Score > 19 indicates homology
NQPKSPITVLIVDDSPFMR + Deamidated (NQ); Oxidation (M)
1914 659.3212 1316.6278 1316.6459 -13.7 1 8 0.77 +1Score > 41 indicates identity
Score > 20 indicates homology
SDNSIPIKLNSE + Deamidated (NQ)
4567 859.4359 3433.7145 3433.6574 16.6 1 8 0.61 +1Score > 35 indicates identity
Score > 19 indicates homology
DVFPCTPMQEALVALSLIPGAQASYALHAAFE + Deamidated (NQ); Oxidation (M)
2696 558.6520 1672.9342 1672.9294 2.85 1 8 0.95 +1Score > 36 indicates identity
Score > 21 indicates homology
VSLQNLSMVLRQLR + Deamidated (NQ); Oxidation (M)
2793 864.4963 1726.9780 1727.0080 -17.3 1 8 0.51 +1Score > 35 indicates identity
Score > 18 indicates homology
VLLASLQGAAITSIKIE + Deamidated (NQ)
2746 568.6254 1702.8544 1702.8275 15.8 0 8 0.5 +1Score > 40 indicates identity
Score > 18 indicates homology
GGFGGIIAVGQGSSNEPR + Deamidated (NQ)
2813 867.4818 1732.9490 1732.9148 19.8 1 8 0.55 +1Score > 37 indicates identity
Score > 18 indicates homology
QELPHIDVWINLAGK + Deamidated (NQ)
3299 685.0502 2052.1288 2052.1215 3.54 0 8 0.62 +1Score > 35 indicates identity
Score > 19 indicates homology
VIQTLADNQINVIVAGLDR + Deamidated (NQ)
4765 719.3777 6465.3338 6465.2374 14.9 1 8 0.21 +1Score > 26 indicates identity
Score > 14 indicates homology
CAALSAIAAALLLAILLAYFIAMHLLGLNWQLQPADGHTFNNGIRTGLPGNDDVATMPSGGK + 6 Deamidated (NQ); Oxidation (M)
1520 378.1978 1131.5716 1131.5673 3.81 0 8 0.9 +1Score > 42 indicates identity
Score > 20 indicates homology
AATNPSTAFPR
2021 683.3273 1364.6400 1364.6242 11.6 1 8 0.86 +1Score > 41 indicates identity
Score > 20 indicates homology
VSAQLDRVMSDE + Oxidation (M)
2169 477.2697 1428.7873 1428.7911 -2.69 1 8 0.87 +1Score > 39 indicates identity
Score > 20 indicates homology
LHRCLDLLVYK
1047 310.1640 927.4702 927.4709 -0.74 1 8 0.95 +1Score > 40 indicates identity
Score > 21 indicates homology
MAGPAPGRR + Oxidation (M)
1766 619.7971 1237.5796 1237.5939 -11.5 0 8 0.81 +1Score > 41 indicates identity
Score > 20 indicates homology
QIASDSPHASPK + Deamidated (NQ)
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