MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3248 1012.5354 2023.0562 2023.0659 -4.79 1 10 0.39 +1Score > 38 indicates identity
Score > 18 indicates homology
MEQSLIPQTPVLPLTAQR + 2 Deamidated (NQ)
1112 475.2656 948.5166 948.5141 2.69 1 10 0.75 +1Score > 40 indicates identity
Score > 21 indicates homology
LGGARYQGK
1847 427.5940 1279.7602 1279.7798 -15.4 1 10 0.56 +1Score > 33 indicates identity
Score > 20 indicates homology
LGRKPIIVCPK
714 395.7096 789.4046 789.4167 -15.3 0 10 0.76 +1Score > 42 indicates identity
Score > 21 indicates homology
QVMVAAR + Oxidation (M)
2195 722.4408 1442.8670 1442.8609 4.27 1 10 0.2 +1Score > 30 indicates identity
Score > 15 indicates homology
KLNALITAAPYIR
1634 394.9010 1181.6812 1181.6768 3.70 1 10 0.73 +1Score > 36 indicates identity
Score > 21 indicates homology
KVQHLTSQLK + Deamidated (NQ)
3444 714.3931 2140.1575 2140.1164 19.2 1 10 0.21 +1Score > 36 indicates identity
Score > 15 indicates homology
VVVDNVSSRDWQPVLANLK + 2 Deamidated (NQ)
2617 820.9222 1639.8298 1639.8279 1.16 0 10 0.75 +1Score > 40 indicates identity
Score > 21 indicates homology
WIAYAQSLTSKPMK + Deamidated (NQ); Oxidation (M)
3154 657.6646 1969.9720 1969.9527 9.78 1 10 0.97 +1Score > 40 indicates identity
Score > 22 indicates homology
LAQNAAAGGASGGGTIQMHKK + 3 Deamidated (NQ)
2146 474.8924 1421.6554 1421.6643 -6.25 0 10 0.7 +1Score > 41 indicates identity
Score > 21 indicates homology
MQDLNLAMDALR + 2 Oxidation (M)
3312 687.3599 2059.0579 2059.0700 -5.87 1 10 0.64 +1Score > 39 indicates identity
Score > 20 indicates homology
AMAIFELLDYIVNEPPPK
3582 1106.5419 2211.0692 2211.0596 4.35 1 10 0.89 +1Score > 39 indicates identity
Score > 22 indicates homology
EITGLWQGSLFNANYDVQR + Deamidated (NQ)
4073 900.4581 2698.3525 2698.3503 0.79 1 10 0.34 +1Score > 38 indicates identity
Score > 17 indicates homology
RSSFAANDLFYGHIVGIDTFAAGLR + Deamidated (NQ)
2819 870.4800 1738.9454 1738.9465 -0.61 0 10 0.43 +1Score > 37 indicates identity
Score > 18 indicates homology
LIGSVDDLLPSLPASSR
3167 991.4585 1980.9024 1980.9164 -7.04 1 10 0.65 +1Score > 38 indicates identity
Score > 20 indicates homology
VQNIGKTSIDDVNVSDFE + 2 Deamidated (NQ)
3555 733.3632 2197.0678 2197.0434 11.1 0 10 0.81 +1Score > 39 indicates identity
Score > 21 indicates homology
DHTKPVQQVLADMTNGGVDR + Deamidated (NQ); Oxidation (M)
2847 882.4940 1762.9734 1763.0080 -19.6 1 10 0.46 +1Score > 36 indicates identity
Score > 19 indicates homology
IIENLKPLLPAGIQDK + 2 Deamidated (NQ)
2303 752.3972 1502.7798 1502.7763 2.39 1 10 0.74 +1Score > 41 indicates identity
Score > 21 indicates homology
ITKVASGLMNTHSK + Deamidated (NQ); Oxidation (M)
2887 894.4825 1786.9504 1786.9200 17.1 1 10 0.74 +1Score > 39 indicates identity
Score > 21 indicates homology
LQSIQADITLQKNLAE + 3 Deamidated (NQ)
3091 638.9905 1913.9497 1913.9703 -10.8 0 10 0.98 +1Score > 40 indicates identity
Score > 22 indicates homology
QALGVGVAQCVAMIPGVSR + 2 Deamidated (NQ)
3600 744.0048 2228.9926 2228.9541 17.3 0 10 0.81 +1Score > 37 indicates identity
Score > 21 indicates homology
LFSFNAPMGACPACNGLGVTE + Deamidated (NQ); Oxidation (M)
3619 1122.1150 2242.2154 2242.1928 10.1 0 10 0.45 +1Score > 35 indicates identity
Score > 19 indicates homology
LFGGMGLMLVFVLGQGMLLSK + 2 Oxidation (M)
3906 828.0811 2481.2215 2481.1880 13.5 1 10 0.26 +1Score > 38 indicates identity
Score > 16 indicates homology
MVIMSEFSAVPSGTGQGQQKPLR + 2 Deamidated (NQ); 2 Oxidation (M)
3590 1111.5228 2221.0310 2221.0069 10.9 1 10 0.71 +1Score > 38 indicates identity
Score > 21 indicates homology
GAFLLCRTSNPGGDDLQNQR + 3 Deamidated (NQ)
3805 794.1039 2379.2899 2379.2885 0.56 0 10 0.14 +1Score > 34 indicates identity
Score > 14 indicates homology
FGIGADGVLFLAKPLHTSLHMR
1787 415.5681 1243.6825 1243.6812 1.00 0 10 0.85 +1Score > 40 indicates identity
Score > 21 indicates homology
GFIDPGAINLVK + Deamidated (NQ)
2618 547.6340 1639.8802 1639.8716 5.26 0 10 0.32 +1Score > 38 indicates identity
Score > 17 indicates homology
LTSHLLAMNLLGDAR + Oxidation (M)
3745 774.4247 2320.2523 2320.2534 -0.51 1 10 0.2 +1Score > 35 indicates identity
Score > 15 indicates homology
MSKIIPNIVVSMPTQLFTLR + Deamidated (NQ); 2 Oxidation (M)
2550 807.4435 1612.8724 1612.8719 0.34 1 10 0.75 +1Score > 39 indicates identity
Score > 21 indicates homology
IARVLTNDPATGVMR
3735 579.0667 2312.2377 2312.2045 14.3 1 10 0.28 +1Score > 36 indicates identity
Score > 16 indicates homology
KQSVPANILSLQLQQMAANLE + Deamidated (NQ); Oxidation (M)
2440 784.4006 1566.7866 1566.7963 -6.18 1 10 0.89 +1Score > 40 indicates identity
Score > 22 indicates homology
VYLSAVVQKMVNSE + Deamidated (NQ)
3123 971.5460 1941.0774 1941.0935 -8.26 0 9 0.58 +1Score > 35 indicates identity
Score > 20 indicates homology
LLIVDRPIDQIAPVIHE + Deamidated (NQ)
1154 483.2948 964.5750 964.5593 16.3 1 9 0.46 +1Score > 34 indicates identity
Score > 19 indicates homology
LVKNYLSK + Deamidated (NQ)
2774 572.3198 1713.9376 1713.9261 6.71 0 9 0.92 +1Score > 38 indicates identity
Score > 22 indicates homology
QLNTNVNTIISILNR + 2 Deamidated (NQ)
3321 1038.5292 2075.0438 2075.0397 1.98 0 9 0.51 +1Score > 40 indicates identity
Score > 19 indicates homology
ADLVLVNTCAFIKPAWQE + Deamidated (NQ)
2872 887.4682 1772.9218 1772.8866 19.9 0 9 0.63 +1Score > 40 indicates identity
Score > 20 indicates homology
LNTILNTMSTIYSTGK + Deamidated (NQ); Oxidation (M)
1168 324.8393 971.4961 971.5076 -11.9 1 9 0.85 +1Score > 41 indicates identity
Score > 21 indicates homology
TFKSYAQK
3645 752.0377 2253.0913 2253.0623 12.8 0 9 0.76 +1Score > 39 indicates identity
Score > 21 indicates homology
STQLQTWDFLPISMNLQGR + 3 Deamidated (NQ); Oxidation (M)
224 506.2669 505.2596 505.2496 19.8 0 9 0.61 +1Score > 44 indicates identity
Score > 20 indicates homology
SSAGGK
2046 458.8911 1373.6515 1373.6497 1.32 1 9 0.93 +1Score > 41 indicates identity
Score > 22 indicates homology
GAVNLMNDPAEVK + Deamidated (NQ); Oxidation (M)
3208 668.0490 2001.1252 2001.1106 7.28 1 9 0.24 +1Score > 35 indicates identity
Score > 16 indicates homology
GAAGSDTKILAVTILTSLDR
863 429.2237 856.4328 856.4290 4.44 0 9 0.78 +1Score > 39 indicates identity
Score > 21 indicates homology
AGVGVSAAPE
1390 357.1586 1068.4540 1068.4546 -0.56 1 9 0.97 +1Score > 38 indicates identity
Score > 22 indicates homology
GADLRYNME + Deamidated (NQ)
2592 816.4200 1630.8254 1630.8025 14.1 1 9 0.95 +1Score > 41 indicates identity
Score > 22 indicates homology
CAFRVLLPSDPVQE + Deamidated (NQ)
3096 959.0094 1916.0042 1915.9673 19.3 1 9 0.56 +1Score > 39 indicates identity
Score > 19 indicates homology
VSIGSQELQIVCGANNVK + Deamidated (NQ)
577 365.2113 728.4080 728.4181 -13.8 0 9 0.97 +1Score > 42 indicates identity
Score > 22 indicates homology
TVSPGLR
2991 617.2753 1848.8041 1848.7869 9.27 1 9 0.24 +1Score > 37 indicates identity
Score > 16 indicates homology
KMSGHMGAVQQSTQNLE + 4 Deamidated (NQ)
2926 602.3272 1803.9598 1803.9513 4.71 1 9 0.57 +1Score > 39 indicates identity
Score > 19 indicates homology
NLTTNGGRVLCITSLGK + Deamidated (NQ)
2873 592.3218 1773.9436 1773.9295 7.94 1 9 0.36 +1Score > 39 indicates identity
Score > 17 indicates homology
GKAMTVSALDGVPGLGASK + Oxidation (M)
1236 499.8006 997.5866 997.5808 5.90 1 9 0.88 +1Score > 38 indicates identity
Score > 21 indicates homology
EKPSLSPLK
2304 752.4156 1502.8166 1502.8457 -19.3 1 9 0.6 +1Score > 39 indicates identity
Score > 20 indicates homology
LSVPRSALPPLAPGE
2616 820.9189 1639.8232 1639.8457 -13.7 0 9 0.95 +1Score > 40 indicates identity
Score > 22 indicates homology
AVQASYNPTIATLYK + Deamidated (NQ)
3742 773.4549 2317.3429 2317.3270 6.84 1 9 0.26 +1Score > 28 indicates identity
Score > 16 indicates homology
ILNIPKLGSVNVHGSILPQYR
1379 531.7679 1061.5212 1061.5175 3.50 0 9 0.78 +1Score > 42 indicates identity
Score > 21 indicates homology
MTSSPSSIPR
3620 748.4153 2242.2241 2242.1917 14.4 1 9 0.5 +1Score > 35 indicates identity
Score > 19 indicates homology
TRQQIQQLDTQLLTAISQR + 2 Deamidated (NQ)
2530 805.3868 1608.7590 1608.7640 -3.06 1 9 0.73 +1Score > 40 indicates identity
Score > 20 indicates homology
MGQVLIAPGEYQMR + Deamidated (NQ); Oxidation (M)
3212 1004.5876 2007.1606 2007.1405 10.1 1 9 0.98 +1Score > 29 indicates identity
Score > 22 indicates homology
IVLGIDPALGSLGWAVVAKE
3416 709.7256 2126.1550 2126.1232 14.9 1 9 0.81 +1Score > 36 indicates identity
Score > 21 indicates homology
QANSGLTHFTAALIQLRQR + 2 Deamidated (NQ)
3368 1050.5535 2099.0924 2099.1118 -9.24 1 9 0.95 +1Score > 38 indicates identity
Score > 22 indicates homology
LKSQIQMVLMQVLEPDAR + Deamidated (NQ)
2196 722.4485 1442.8824 1442.9112 -19.9 0 9 0.16 +1Score > 26 indicates identity
Score > 14 indicates homology
AIIQLIIVGYVLK + Deamidated (NQ)
2199 482.6024 1444.7854 1444.7569 19.7 1 9 0.76 +1Score > 39 indicates identity
Score > 21 indicates homology
RIQNAGGSVMIQR + Oxidation (M)
2914 451.4585 1801.8049 1801.7966 4.60 1 9 0.32 +1Score > 38 indicates identity
Score > 17 indicates homology
TASKDDAGASDDHASLLE
4024 878.4546 2632.3420 2632.3101 12.1 1 9 0.31 +1Score > 37 indicates identity
Score > 17 indicates homology
QSGVNPIVVHGGGPQIGAMLNRMGIE + 2 Deamidated (NQ)
3296 513.2545 2048.9889 2048.9684 9.98 0 9 0.47 +1Score > 40 indicates identity
Score > 18 indicates homology
MSAQDAAGLSLSDGDQVLVR + Deamidated (NQ); Oxidation (M)
3148 656.3419 1966.0039 1966.0054 -0.79 1 9 0.93 +1Score > 39 indicates identity
Score > 21 indicates homology
QIHLPAQSGSSRVLDAMR + Deamidated (NQ)
2939 904.9932 1807.9718 1807.9978 -14.4 1 9 0.72 +1Score > 37 indicates identity
Score > 20 indicates homology
LSLPGCLPPLSGSQVKR
3262 1018.0427 2034.0708 2034.0860 -7.43 1 9 0.82 +1Score > 38 indicates identity
Score > 21 indicates homology
AIFNQDILPFKDLIAMGK + Deamidated (NQ)
3477 718.7177 2153.1313 2153.1633 -14.9 0 9 0.34 +1Score > 38 indicates identity
Score > 17 indicates homology
RPQIQTIAPGAQIAHFLYK + 2 Deamidated (NQ)
2014 455.2311 1362.6715 1362.6528 13.7 1 9 0.5 +1Score > 41 indicates identity
Score > 19 indicates homology
VGSAVGAAAGGEFDR
555 361.2173 720.4200 720.4204 -0.46 1 9 0.97 +1Score > 38 indicates identity
Score > 22 indicates homology
MSKIVK + Oxidation (M)
675 388.2328 774.4510 774.4487 3.05 0 9 0.87 +1Score > 39 indicates identity
Score > 21 indicates homology
LSNSIIK + Deamidated (NQ)
2091 465.8965 1394.6677 1394.6466 15.1 0 9 0.84 +1Score > 41 indicates identity
Score > 21 indicates homology
WQSLLNFNSQR + 3 Deamidated (NQ)
1429 362.8777 1085.6113 1085.6154 -3.85 0 9 0.57 +1Score > 40 indicates identity
Score > 19 indicates homology
ITAPLMPLSK + Oxidation (M)
3033 623.3433 1867.0081 1867.0237 -8.37 0 9 0.79 +1Score > 37 indicates identity
Score > 21 indicates homology
TIATLLNANVTPCLPLR + Deamidated (NQ)
3863 809.4251 2425.2535 2425.2246 11.9 1 9 0.73 +1Score > 37 indicates identity
Score > 20 indicates homology
AKNVVGLSAMNFGTFPMVNGLSR + Oxidation (M)
2487 529.6303 1585.8691 1585.8940 -15.7 1 9 0.81 +1Score > 38 indicates identity
Score > 21 indicates homology
VLGTGVWLDTTRLR
2831 877.4675 1752.9204 1752.9482 -15.8 1 9 0.72 +1Score > 39 indicates identity
Score > 20 indicates homology
IDTALRGASDILAPQGR
2967 610.6911 1829.0515 1829.0159 19.4 0 9 0.99 +1Score > 32 indicates identity
Score > 22 indicates homology
ALAQGSDYLVIGRPITR
3119 484.5199 1934.0505 1934.0333 8.87 1 9 0.49 +1Score > 36 indicates identity
Score > 19 indicates homology
SAHRSSLGNVLQADLPIR + Deamidated (NQ)
3701 767.7582 2300.2528 2300.2450 3.39 1 9 0.32 +1Score > 35 indicates identity
Score > 17 indicates homology
VQTAITMGQVAEPYIPLKAIR + 2 Deamidated (NQ)
2194 722.4151 1442.8156 1442.7868 20.0 1 9 0.92 +1Score > 37 indicates identity
Score > 21 indicates homology
LQQLQKQILTVE + 3 Deamidated (NQ)
2405 775.9406 1549.8666 1549.8617 3.21 1 9 0.99 +1Score > 36 indicates identity
Score > 22 indicates homology
LFGIGNVSRVFVDK
2449 524.9505 1571.8297 1571.8519 -14.1 1 9 1 +1Score > 40 indicates identity
Score > 22 indicates homology
DITQLLNIAESSLR
2977 613.9780 1838.9122 1838.8839 15.4 1 9 0.48 +1Score > 40 indicates identity
Score > 18 indicates homology
ALWISFNPEGLSAHPAE + Deamidated (NQ)
3087 478.5023 1909.9801 1909.9997 -10.2 0 9 0.99 +1Score > 39 indicates identity
Score > 22 indicates homology
IVSGGTDNHLLLVDLTNK + 2 Deamidated (NQ)
3341 1043.5347 2085.0548 2085.0466 3.96 1 9 0.49 +1Score > 39 indicates identity
Score > 19 indicates homology
YHGLSVGVILNTMHPFER + Oxidation (M)
3649 754.3737 2260.0993 2260.0794 8.80 1 9 0.91 +1Score > 39 indicates identity
Score > 21 indicates homology
HLIDAFNMNTTNVTVGDLRE + Deamidated (NQ)
745 403.2318 804.4490 804.4527 -4.57 1 9 0.84 +1Score > 41 indicates identity
Score > 21 indicates homology
IQKMIR + Deamidated (NQ); Oxidation (M)
3247 1012.5323 2023.0500 2023.0698 -9.75 1 9 0.51 +1Score > 38 indicates identity
Score > 19 indicates homology
SAALQSIQENLGLQDAPLR
1108 948.5016 947.4943 947.4858 8.97 1 9 0.91 +1Score > 42 indicates identity
Score > 21 indicates homology
QIQKMQR + Deamidated (NQ); Oxidation (M)
2434 782.3887 1562.7628 1562.7763 -8.58 1 9 0.83 +1Score > 41 indicates identity
Score > 21 indicates homology
TLVGQMGAAYPELGR + Deamidated (NQ)
2615 547.2990 1638.8752 1638.8729 1.36 1 9 0.93 +1Score > 38 indicates identity
Score > 21 indicates homology
LDGALASVVPWLNER
1368 353.5284 1057.5634 1057.5768 -12.7 1 9 0.67 +1Score > 42 indicates identity
Score > 20 indicates homology
KAQQVLGVSE
3066 946.4813 1890.9480 1890.9218 13.9 1 9 0.81 +1Score > 40 indicates identity
Score > 21 indicates homology
DDVAASMALRIAGSSGQAR + Oxidation (M)
3204 667.3334 1998.9784 1998.9721 3.16 1 9 0.88 +1Score > 40 indicates identity
Score > 21 indicates homology
LLGGHMLDQFLPVRQQE + 3 Deamidated (NQ); Oxidation (M)
648 382.7134 763.4122 763.4228 -13.8 0 9 0.76 +1Score > 41 indicates identity
Score > 20 indicates homology
ANVGLYK
2066 462.6083 1384.8031 1384.7813 15.7 0 9 0.98 +1Score > 36 indicates identity
Score > 21 indicates homology
GLTIDDITPSLLK
3672 1139.5918 2277.1690 2277.1398 12.9 1 9 0.51 +1Score > 38 indicates identity
Score > 19 indicates homology
DAILMNMPNAVISLHRYFR + Deamidated (NQ); Oxidation (M)
4459 803.4133 3209.6241 3209.5836 12.6 0 9 0.64 +1Score > 35 indicates identity
Score > 20 indicates homology
QTLFTQQSLTNMLSQLGITVPQGSNMQLK + 4 Deamidated (NQ)
776 412.7745 823.5344 823.5392 -5.75 1 9 0.47 +1Score > 28 indicates identity
Score > 18 indicates homology
GRIPLLR
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