MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2342 758.9310 1515.8474 1515.8330 9.51 1 10 0.72 +1Score > 37 indicates identity
Score > 21 indicates homology
VNAAALIEMVLTGAK + Oxidation (M)
2494 531.2817 1590.8233 1590.8293 -3.81 0 10 0.58 +1Score > 40 indicates identity
Score > 20 indicates homology
YSLDSYLISVYIR
3314 687.3615 2059.0627 2059.0884 -12.5 1 10 0.88 +1Score > 39 indicates identity
Score > 22 indicates homology
ANLVFGGTNLQIVMVPGRR + 2 Deamidated (NQ); Oxidation (M)
1842 639.3723 1276.7300 1276.7292 0.67 1 10 0.28 +1Score > 35 indicates identity
Score > 17 indicates homology
KALFAPFQVTR
2092 465.8992 1394.6758 1394.6929 -12.3 1 10 0.48 +1Score > 41 indicates identity
Score > 20 indicates homology
SSELNTIVQQFK + 2 Deamidated (NQ)
2231 735.3715 1468.7284 1468.7020 18.0 1 10 0.48 +1Score > 40 indicates identity
Score > 20 indicates homology
ANQLPMFQEFTK + Oxidation (M)
889 434.2054 866.3962 866.3878 9.78 0 10 0.73 +1Score > 41 indicates identity
Score > 21 indicates homology
AMLGVMTE + Oxidation (M)
2940 906.0369 1810.0592 1810.0703 -6.10 1 10 0.39 +1Score > 30 indicates identity
Score > 19 indicates homology
VLTKNVILDNILLVLE + 2 Deamidated (NQ)
214 489.2800 488.2727 488.2707 4.16 0 10 0.93 +1Score > 44 indicates identity
Score > 23 indicates homology
SGGIR
3575 553.0548 2208.1901 2208.1903 -0.082 1 10 0.27 +1Score > 36 indicates identity
Score > 17 indicates homology
QTPDHDLLVVVKQTAFIQR + Deamidated (NQ)
1952 666.3892 1330.7638 1330.7456 13.7 1 10 0.68 +1Score > 35 indicates identity
Score > 21 indicates homology
ILLTSIDKDGTR
3926 835.4428 2503.3066 2503.3434 -14.7 0 10 0.58 +1Score > 36 indicates identity
Score > 20 indicates homology
LVQQPQNASTFINRPALGILPPE + Deamidated (NQ)
2522 803.9440 1605.8734 1605.8800 -4.07 1 10 0.51 +1Score > 37 indicates identity
Score > 20 indicates homology
AFVVIQAPSASMLKK + Deamidated (NQ); Oxidation (M)
1499 562.8240 1123.6334 1123.6390 -4.91 1 10 0.39 +1Score > 36 indicates identity
Score > 19 indicates homology
SYGIKPFGKK
2149 474.9362 1421.7868 1421.7813 3.85 0 10 0.8 +1Score > 37 indicates identity
Score > 22 indicates homology
AKPLMPGGVNSPVR
1056 465.3093 928.6040 928.5957 9.00 1 10 0.56 +1Score > 30 indicates identity
Score > 20 indicates homology
LVKSLIQK + Deamidated (NQ)
3365 700.0080 2097.0022 2097.0122 -4.79 0 10 0.28 +1Score > 39 indicates identity
Score > 17 indicates homology
AMLDSLNIPYVIDTNMVR + Deamidated (NQ); 2 Oxidation (M)
1165 971.4993 970.4920 970.4971 -5.23 0 10 0.55 +1Score > 40 indicates identity
Score > 20 indicates homology
DPAQGQLLK + 2 Deamidated (NQ)
1690 403.1967 1206.5683 1206.5663 1.66 1 10 0.96 +1Score > 41 indicates identity
Score > 23 indicates homology
EMVNQSTNLR + Oxidation (M)
2413 778.4412 1554.8678 1554.8869 -12.2 1 10 0.99 +1Score > 37 indicates identity
Score > 23 indicates homology
DKIIVQSVPDVTLK + Deamidated (NQ)
3948 841.7896 2522.3470 2522.3090 15.1 1 10 0.41 +1Score > 35 indicates identity
Score > 19 indicates homology
SLTLSHNNMIALKPILQAWLEE + 2 Deamidated (NQ)
1446 549.2761 1096.5376 1096.5587 -19.2 0 10 0.72 +1Score > 40 indicates identity
Score > 21 indicates homology
TLFAMQTLR + Deamidated (NQ); Oxidation (M)
2744 568.3371 1701.9895 1701.9890 0.30 1 10 0.15 +1Score > 31 indicates identity
Score > 14 indicates homology
IVYQNLTRINISIR
3287 682.3279 2043.9619 2043.9803 -9.00 0 10 0.43 +1Score > 39 indicates identity
Score > 19 indicates homology
LLPFHADPTYFHQSSQR + Deamidated (NQ)
3433 712.3852 2134.1338 2134.1270 3.19 0 10 0.17 +1Score > 37 indicates identity
Score > 15 indicates homology
TLLLGDNQLSQLSPHVGALR + 3 Deamidated (NQ)
3922 834.0732 2499.1978 2499.2176 -7.94 0 10 0.12 +1Score > 39 indicates identity
Score > 13 indicates homology
GGCLNLSQSNVFLDLVQGANHQK + Deamidated (NQ)
2273 498.9252 1493.7538 1493.7337 13.5 0 10 0.65 +1Score > 41 indicates identity
Score > 21 indicates homology
GIGPWTANYVLMR + Deamidated (NQ); Oxidation (M)
1132 479.2561 956.4976 956.4927 5.18 0 10 0.95 +1Score > 40 indicates identity
Score > 22 indicates homology
TITAHAQSK + Deamidated (NQ)
1874 647.9128 1293.8110 1293.8271 -12.4 0 10 0.97 +1Score > 27 indicates identity
Score > 23 indicates homology
ILLPAISDVILK
3500 1086.5598 2171.1050 2171.1434 -17.6 1 10 0.37 +1Score > 39 indicates identity
Score > 18 indicates homology
DVDILLNNGADKVSINSSAVK
3200 999.9967 1997.9788 1997.9979 -9.56 1 10 0.2 +1Score > 40 indicates identity
Score > 16 indicates homology
LQLVDKNVNIMHTIVDE + 2 Deamidated (NQ); Oxidation (M)
2726 566.6240 1696.8502 1696.8681 -10.5 1 10 0.57 +1Score > 40 indicates identity
Score > 20 indicates homology
RFNFALMVVMVQLE + Deamidated (NQ)
694 783.4023 782.3950 782.3851 12.7 1 10 0.31 +1Score > 39 indicates identity
Score > 18 indicates homology
EDFLIF
3448 714.7219 2141.1439 2141.1820 -17.8 1 10 0.22 +1Score > 36 indicates identity
Score > 16 indicates homology
RGADTIVVVPLLFAAGMHFK
2686 835.5048 1668.9950 1668.9662 17.3 1 10 0.93 +1Score > 29 indicates identity
Score > 22 indicates homology
VSLADLIVLAGTAAVEK
2923 902.4147 1802.8148 1802.8179 -1.67 1 10 0.9 +1Score > 39 indicates identity
Score > 22 indicates homology
VTDMMYNLVNKAQSR + 2 Deamidated (NQ); 2 Oxidation (M)
3062 629.6765 1886.0077 1885.9897 9.51 1 10 0.64 +1Score > 38 indicates identity
Score > 21 indicates homology
SWQLTLNNDIKLNLGR + 2 Deamidated (NQ)
473 343.7148 685.4150 685.4235 -12.3 1 10 0.75 +1Score > 41 indicates identity
Score > 21 indicates homology
NAVVKR
1986 450.8967 1349.6683 1349.6431 18.6 1 10 0.88 +1Score > 41 indicates identity
Score > 22 indicates homology
LPQNAMSRTMGK + Deamidated (NQ); Oxidation (M)
1900 655.3310 1308.6474 1308.6422 4.01 1 10 0.96 +1Score > 41 indicates identity
Score > 22 indicates homology
KSANNAHQVPDK + Deamidated (NQ)
2197 722.8676 1443.7206 1443.7205 0.089 1 10 0.81 +1Score > 41 indicates identity
Score > 22 indicates homology
QGDNLQVTIAEVR + 2 Deamidated (NQ)
3157 658.0423 1971.1051 1971.0789 13.3 0 10 1 +1Score > 34 indicates identity
Score > 23 indicates homology
IVNHLHIPIQSGSNTVLK + 2 Deamidated (NQ)
2484 529.6257 1585.8553 1585.8311 15.2 0 10 0.89 +1Score > 39 indicates identity
Score > 22 indicates homology
LSAQQLQTNVNVLR + 3 Deamidated (NQ)
2602 818.4415 1634.8684 1634.8529 9.52 1 10 0.63 +1Score > 39 indicates identity
Score > 21 indicates homology
NREPVVAATQFFTR
3824 799.4211 2395.2415 2395.1941 19.8 1 10 0.45 +1Score > 37 indicates identity
Score > 19 indicates homology
DLDIVVVNAGTPQELDAMLPAGR + 2 Deamidated (NQ)
693 783.4016 782.3943 782.3851 11.8 1 10 0.28 +1Score > 39 indicates identity
Score > 17 indicates homology
EDFLIF
1304 343.1946 1026.5620 1026.5498 11.8 1 10 0.35 +1Score > 39 indicates identity
Score > 18 indicates homology
RVYDTFVK
2119 469.5998 1405.7776 1405.7565 15.0 0 10 0.71 +1Score > 37 indicates identity
Score > 21 indicates homology
SQQVVIFDTTLR
3804 794.1036 2379.2890 2379.2885 0.18 0 10 0.16 +1Score > 34 indicates identity
Score > 15 indicates homology
FGIGADGVLFLAKPLHTSLHMR
2406 775.9651 1549.9156 1549.9079 4.99 0 10 0.81 +1Score > 32 indicates identity
Score > 22 indicates homology
KPQVIINAINLLVD + Deamidated (NQ)
3313 1030.5363 2059.0580 2059.0321 12.6 1 10 0.34 +1Score > 39 indicates identity
Score > 18 indicates homology
DTRISGDILLQAITQGINE + 3 Deamidated (NQ)
520 352.2224 702.4302 702.4276 3.80 1 10 0.87 +1Score > 38 indicates identity
Score > 22 indicates homology
VKDTIK
3769 784.0541 2349.1405 2349.1852 -19.1 1 10 0.37 +1Score > 39 indicates identity
Score > 18 indicates homology
SINPDEAVQYGAAVQAFILTGGK + Deamidated (NQ)
2669 834.4561 1666.8976 1666.8712 15.9 1 10 0.57 +1Score > 38 indicates identity
Score > 20 indicates homology
QIMEQHQAAIIIQK + Deamidated (NQ); Oxidation (M)
2364 765.4370 1528.8594 1528.8613 -1.19 1 10 0.78 +1Score > 37 indicates identity
Score > 21 indicates homology
KLLAAFPNLANLSR + 2 Deamidated (NQ)
273 563.2853 562.2780 562.2711 12.3 0 10 0.78 +1Score > 39 indicates identity
Score > 21 indicates homology
QGSSGK
530 354.6886 707.3626 707.3602 3.40 0 10 0.96 +1Score > 43 indicates identity
Score > 22 indicates homology
FTTPSR
2300 752.3915 1502.7684 1502.7437 16.4 1 10 0.91 +1Score > 41 indicates identity
Score > 22 indicates homology
LTSDGAGISGGQRQR + Deamidated (NQ)
3022 622.6704 1864.9894 1864.9717 9.50 0 10 0.67 +1Score > 38 indicates identity
Score > 21 indicates homology
SVPGYSNIITAIGMLASR + Oxidation (M)
600 371.7642 741.5138 741.5112 3.53 1 10 0.67 +1Score > 31 indicates identity
Score > 21 indicates homology
IGALLKK
1352 349.8418 1046.5036 1046.5179 -13.7 1 10 0.97 +1Score > 42 indicates identity
Score > 22 indicates homology
KGTPGDLMGR + Oxidation (M)
3504 1087.0496 2172.0846 2172.0771 3.49 1 10 0.87 +1Score > 39 indicates identity
Score > 22 indicates homology
QNTRPVSEVNASLDLNTASR + Deamidated (NQ)
1190 490.7675 979.5204 979.5338 -13.7 1 10 0.96 +1Score > 39 indicates identity
Score > 22 indicates homology
LVNPPPKSE
3074 633.9994 1898.9764 1898.9697 3.50 1 10 0.28 +1Score > 39 indicates identity
Score > 17 indicates homology
TPSANQIIKNLSGGNQQK + 2 Deamidated (NQ)
3244 1012.5292 2023.0438 2023.0586 -7.28 1 10 0.53 +1Score > 39 indicates identity
Score > 20 indicates homology
LGGGPGAGDLQTLALEVAQQK + Deamidated (NQ)
3589 740.3979 2218.1719 2218.1674 2.03 1 10 0.85 +1Score > 37 indicates identity
Score > 22 indicates homology
GRPFILLAFLNNPEFQVLE + 2 Deamidated (NQ)
3851 605.3204 2417.2525 2417.2672 -6.06 1 10 0.35 +1Score > 37 indicates identity
Score > 18 indicates homology
SLHPHVPGVTNLQVMRAMASLK + 2 Oxidation (M)
1454 551.2994 1100.5842 1100.5826 1.51 1 10 0.87 +1Score > 41 indicates identity
Score > 22 indicates homology
IVDEGATIQR
1875 647.9136 1293.8126 1293.8271 -11.2 0 10 0.47 +1Score > 27 indicates identity
Score > 19 indicates homology
ILLPAISDVILK
3025 934.0256 1866.0366 1866.0211 8.35 1 10 0.54 +1Score > 35 indicates identity
Score > 20 indicates homology
DGVIGDDNRIVAALPTIK
3425 710.7112 2129.1118 2129.0991 5.96 1 10 0.9 +1Score > 38 indicates identity
Score > 22 indicates homology
SIVDLSDNDIINIVLEDLK + 2 Deamidated (NQ)
3625 561.8083 2243.2041 2243.1620 18.8 1 10 0.27 +1Score > 36 indicates identity
Score > 17 indicates homology
TGTLTTNHMIVDKVWIGDVK + Oxidation (M)
3777 786.7619 2357.2639 2357.2267 15.8 1 10 0.18 +1Score > 36 indicates identity
Score > 15 indicates homology
NLYIIYSDFTVNKNIQLAAR + 2 Deamidated (NQ)
1733 409.5461 1225.6165 1225.6244 -6.45 1 10 0.76 +1Score > 40 indicates identity
Score > 21 indicates homology
QDKYAIWFR
2047 458.8925 1373.6557 1373.6364 14.0 1 10 0.87 +1Score > 41 indicates identity
Score > 22 indicates homology
ENYIPDSFPHR
2641 826.4415 1650.8684 1650.8624 3.66 0 10 0.35 +1Score > 39 indicates identity
Score > 18 indicates homology
SVVLMSHLGRPDGQR
2339 758.3716 1514.7286 1514.7188 6.52 1 10 0.38 +1Score > 41 indicates identity
Score > 18 indicates homology
FTGGLEMLFSNQR + Oxidation (M)
2851 588.9944 1763.9614 1763.9491 6.95 1 10 0.4 +1Score > 36 indicates identity
Score > 18 indicates homology
YNVIVKGLAAKPMTNK + 2 Deamidated (NQ); Oxidation (M)
3384 704.3727 2110.0963 2110.0921 1.98 1 10 0.37 +1Score > 38 indicates identity
Score > 18 indicates homology
LLFNDIIFRMPNPNYVK + Deamidated (NQ); Oxidation (M)
1030 462.7698 923.5250 923.5076 18.9 0 10 0.63 +1Score > 37 indicates identity
Score > 20 indicates homology
LVLVHGSAE
2644 552.2941 1653.8605 1653.8587 1.08 1 10 0.9 +1Score > 39 indicates identity
Score > 22 indicates homology
AYGGRATTVVNNYIR
3073 632.3521 1894.0345 1893.9989 18.8 1 10 0.95 +1Score > 36 indicates identity
Score > 22 indicates homology
ETPHVPIPYVYPVDLR
2042 457.9179 1370.7319 1370.7293 1.86 0 10 0.95 +1Score > 40 indicates identity
Score > 22 indicates homology
VVDIGVQQGLVDK + 2 Deamidated (NQ)
1456 368.5263 1102.5571 1102.5441 11.8 1 10 0.85 +1Score > 42 indicates identity
Score > 22 indicates homology
MNISKPEQR + Deamidated (NQ)
2694 837.4673 1672.9200 1672.9069 7.84 1 10 0.39 +1Score > 37 indicates identity
Score > 18 indicates homology
KLVILGGAMQGNVLDK + 2 Deamidated (NQ); Oxidation (M)
1477 372.5354 1114.5844 1114.6029 -16.7 1 10 0.99 +1Score > 41 indicates identity
Score > 22 indicates homology
KIGMGRPGQR + Oxidation (M)
2548 807.4351 1612.8556 1612.8461 5.94 1 10 0.3 +1Score > 39 indicates identity
Score > 17 indicates homology
KVNSAVFPGLQGGPLE + Deamidated (NQ)
1072 313.8356 938.4850 938.4821 3.01 1 10 0.82 +1Score > 39 indicates identity
Score > 21 indicates homology
VPDPGEGLR
2811 578.6560 1732.9462 1732.9216 14.2 1 10 0.39 +1Score > 37 indicates identity
Score > 18 indicates homology
VLDLGCGAGVIGSMIKK + Oxidation (M)
3499 1086.5546 2171.0946 2171.0820 5.82 0 10 0.45 +1Score > 39 indicates identity
Score > 19 indicates homology
VAIDNGLMLLDPVGQLFNPE + Deamidated (NQ); Oxidation (M)
247 529.3476 528.3403 528.3384 3.71 0 10 0.86 +1Score > 41 indicates identity
Score > 22 indicates homology
IGIAR
499 351.2039 700.3932 700.3868 9.23 0 10 0.95 +1Score > 42 indicates identity
Score > 22 indicates homology
GLAQQGK
1255 504.2619 1006.5092 1006.5117 -2.47 1 10 0.84 +1Score > 42 indicates identity
Score > 21 indicates homology
STGVCKQVK + Deamidated (NQ)
3263 679.0309 2034.0709 2034.0707 0.080 1 10 0.35 +1Score > 38 indicates identity
Score > 18 indicates homology
TLGLLQLMAQAGLFITAEE + Oxidation (M)
2778 860.4284 1718.8422 1718.8628 -11.9 1 10 0.7 +1Score > 41 indicates identity
Score > 21 indicates homology
NVITENPFTYVSHAK
316 305.6883 609.3620 609.3598 3.61 0 10 0.21 +1Score > 34 indicates identity
Score > 15 indicates homology
AGVHVK
376 322.1881 642.3616 642.3561 8.57 1 10 0.72 +1Score > 35 indicates identity
Score > 21 indicates homology
AGRSPR
1125 956.4801 955.4728 955.4732 -0.35 1 10 0.62 +1Score > 40 indicates identity
Score > 20 indicates homology
MFSMLRR + Oxidation (M)
2018 455.2509 1362.7309 1362.7256 3.90 1 10 0.4 +1Score > 39 indicates identity
Score > 18 indicates homology
EPVADPKGLGQPR
3213 670.0616 2007.1630 2007.1364 13.2 1 10 0.19 +1Score > 29 indicates identity
Score > 15 indicates homology
DDLLIQALTVAVQVPQRK + Deamidated (NQ)
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