MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2054 690.3365 1378.6584 1378.6585 -0.022 1 11 0.4 +1Score > 41 indicates identity
Score > 20 indicates homology		 GQTLQVGEMICK + Oxidation (M)
2531 537.3427 1609.0063 1608.9789 17.0 0 11 0.56 +1Score > 21 indicates identity
Score > 21 indicates homology		 SPCLLLLWLLLLR
2683 557.3372 1668.9898 1668.9661 14.2 1 11 0.79 +1Score > 30 indicates identity
Score > 23 indicates homology		 LNIILQGNIILSTEK + Deamidated (NQ)
2302 752.3923 1502.7700 1502.7437 17.5 1 11 0.89 +1Score > 41 indicates identity
Score > 23 indicates homology		 LTSDGAGISGGQRQR + Deamidated (NQ)
2387 515.2879 1542.8419 1542.8253 10.7 1 11 0.96 +1Score > 39 indicates identity
Score > 23 indicates homology		 SLISSGQPLGLEAGSK
3251 676.3677 2026.0813 2026.0775 1.85 1 11 0.19 +1Score > 37 indicates identity
Score > 16 indicates homology		 GVHVFILDPSLGEFILAAE
803 836.4243 835.4170 835.4076 11.3 1 11 0.92 +1Score > 42 indicates identity
Score > 23 indicates homology		 QFGEINK + Deamidated (NQ)
1659 598.3056 1194.5966 1194.6166 -16.7 0 11 0.91 +1Score > 41 indicates identity
Score > 23 indicates homology		 MSTITVDLATK + Oxidation (M)
557 361.7026 721.3906 721.3793 15.8 0 11 0.79 +1Score > 40 indicates identity
Score > 23 indicates homology		 MSITVR + Oxidation (M)
3503 1087.0470 2172.0794 2172.0409 17.8 1 11 0.94 +1Score > 39 indicates identity
Score > 23 indicates homology		 NTMYAQVFNLQGKTPVSQK + 3 Deamidated (NQ); Oxidation (M)
1742 614.8390 1227.6634 1227.6645 -0.86 1 11 0.95 +1Score > 40 indicates identity
Score > 23 indicates homology		 KNPMVLGNINK + Deamidated (NQ)
3623 748.4192 2242.2358 2242.1917 19.7 1 11 0.36 +1Score > 34 indicates identity
Score > 19 indicates homology		 TRQQIQQLDTQLLTAISQR + 2 Deamidated (NQ)
3868 813.1094 2436.3064 2436.3095 -1.28 1 11 0.11 +1Score > 35 indicates identity
Score > 14 indicates homology		 ILGIMLLSMHNLLFYNRLMK + Deamidated (NQ); Oxidation (M)
1854 427.8836 1280.6290 1280.6322 -2.52 1 11 0.65 +1Score > 41 indicates identity
Score > 22 indicates homology		 MISYLKGNPIE + Deamidated (NQ); Oxidation (M)
1898 437.2222 1308.6448 1308.6449 -0.084 1 11 0.92 +1Score > 41 indicates identity
Score > 23 indicates homology		 DLQTEINSFLK + 2 Deamidated (NQ)
2051 459.9141 1376.7205 1376.6969 17.1 1 11 0.43 +1Score > 40 indicates identity
Score > 20 indicates homology		 KIANIMSQLSDR + 2 Deamidated (NQ)
1844 427.5648 1279.6726 1279.6659 5.18 0 11 0.96 +1Score > 40 indicates identity
Score > 23 indicates homology		 QQALSLYQSLK + 2 Deamidated (NQ)
1961 668.8309 1335.6472 1335.6340 9.91 1 11 0.58 +1Score > 41 indicates identity
Score > 21 indicates homology		 VVQMTNDDIKR + 2 Deamidated (NQ); Oxidation (M)
3216 670.3397 2007.9973 2007.9823 7.45 1 11 0.98 +1Score > 40 indicates identity
Score > 23 indicates homology		 DPTITLGLATGGTPQKMYE + Oxidation (M)
2181 718.9117 1435.8088 1435.8035 3.76 1 11 0.71 +1Score > 36 indicates identity
Score > 22 indicates homology		 LPDPAKPTESIIR
3415 709.7117 2126.1133 2126.1405 -12.8 1 11 0.81 +1Score > 38 indicates identity
Score > 23 indicates homology		 NLCDILANLKQLNVLDLR + 2 Deamidated (NQ)
3608 1117.6467 2233.2788 2233.2583 9.21 1 11 0.64 +1Score > 29 indicates identity
Score > 22 indicates homology		 SLTRFGFTLIIGGAIGNLIDR
3370 1051.0140 2100.0134 2100.0415 -13.4 1 11 0.28 +1Score > 39 indicates identity
Score > 18 indicates homology		 LLSQPFNSSTPVLYYNKE + Deamidated (NQ)
3403 530.2808 2117.0941 2117.0905 1.68 0 11 0.93 +1Score > 39 indicates identity
Score > 23 indicates homology		 QYIAQHFVAISTNANDVVK
2422 779.8900 1557.7654 1557.7674 -1.28 1 11 0.47 +1Score > 41 indicates identity
Score > 20 indicates homology		 TNTIPNWIKLNNE + 2 Deamidated (NQ)
3295 682.6954 2045.0644 2045.0629 0.74 1 11 0.98 +1Score > 38 indicates identity
Score > 23 indicates homology		 MAKAHDLIAWNAPHSLVR + Oxidation (M)
3456 716.0577 2145.1513 2145.1695 -8.49 1 11 0.46 +1Score > 36 indicates identity
Score > 20 indicates homology		 LFTRPGLKAAVVNLDDGFGR
799 834.4142 833.4069 833.4171 -12.2 0 11 0.7 +1Score > 42 indicates identity
Score > 22 indicates homology		 LPIAYQE + Deamidated (NQ)
1749 410.8685 1229.5837 1229.5928 -7.45 1 11 0.87 +1Score > 40 indicates identity
Score > 23 indicates homology		 VGVALGVGEDWE
945 447.7393 893.4640 893.4607 3.79 1 11 0.65 +1Score > 41 indicates identity
Score > 22 indicates homology		 LSGELYGR
2333 379.2161 1512.8353 1512.8083 17.9 1 11 0.59 +1Score > 38 indicates identity
Score > 21 indicates homology		 KIVCTPGQGLGDLR
1089 471.7763 941.5380 941.5545 -17.5 1 11 0.72 +1Score > 40 indicates identity
Score > 22 indicates homology		 QAVEAILAK
912 440.2526 878.4906 878.4861 5.14 1 11 0.3 +1Score > 38 indicates identity
Score > 18 indicates homology		 KAAVQYAK + Deamidated (NQ)
2464 789.4307 1576.8468 1576.8395 4.65 1 11 0.61 +1Score > 38 indicates identity
Score > 21 indicates homology		 FLGNLMQLLNNKR + Deamidated (NQ); Oxidation (M)
4078 676.6380 2702.5229 2702.4690 19.9 1 11 0.14 +1Score > 29 indicates identity
Score > 15 indicates homology		 GVIVRPVANYGMPNHLRITIGLPAE + Oxidation (M)
2043 458.2280 1371.6622 1371.6526 6.95 1 11 0.96 +1Score > 40 indicates identity
Score > 23 indicates homology		 MMYKDVTQALR + Deamidated (NQ); Oxidation (M)
3166 990.9926 1979.9706 1979.9802 -4.81 0 11 0.54 +1Score > 40 indicates identity
Score > 21 indicates homology		 NVDIVIVSIFINPMQFE + 3 Deamidated (NQ)
3508 725.3676 2173.0810 2173.0804 0.28 1 11 0.37 +1Score > 39 indicates identity
Score > 19 indicates homology		 DKIHHVDSLHQLFSAISPE + Deamidated (NQ)
2987 615.6411 1843.9015 1843.9315 -16.3 1 11 0.86 +1Score > 40 indicates identity
Score > 23 indicates homology		 SANLVINNWNQQIKAK + 4 Deamidated (NQ)
3676 760.4053 2278.1941 2278.1488 19.9 1 11 0.83 +1Score > 37 indicates identity
Score > 23 indicates homology		 QHADLQRQSADALLAMLPQR + Deamidated (NQ); Oxidation (M)
3282 681.0262 2040.0568 2040.0833 -13.0 1 11 0.29 +1Score > 39 indicates identity
Score > 18 indicates homology		 VLPLPEANFPSFPLPHHK + Deamidated (NQ)
4040 663.0623 2648.2201 2648.2581 -14.3 1 11 0.17 +1Score > 37 indicates identity
Score > 16 indicates homology		 MPPSPLSSGAFYQRWLGPVLANDE + Deamidated (NQ); Oxidation (M)
1689 604.2844 1206.5542 1206.5768 -18.7 1 11 0.84 +1Score > 41 indicates identity
Score > 23 indicates homology		 LDVFAGQEISE
2164 714.8943 1427.7740 1427.7508 16.3 0 11 0.85 +1Score > 39 indicates identity
Score > 23 indicates homology		 TGLADADVSEPLIK
713 395.7093 789.4040 789.3981 7.57 1 11 0.45 +1Score > 42 indicates identity
Score > 20 indicates homology		 STGAVEAR
3646 564.2809 2253.0945 2253.0922 1.03 1 11 0.93 +1Score > 39 indicates identity
Score > 23 indicates homology		 KADIACLTSNAGLFAWMDLR + Deamidated (NQ)
3290 512.0320 2044.0989 2044.0629 17.6 1 11 0.63 +1Score > 37 indicates identity
Score > 21 indicates homology		 FINAALNQELFVSIAQHK + 2 Deamidated (NQ)
4003 862.4517 2584.3333 2584.3393 -2.34 1 11 0.4 +1Score > 37 indicates identity
Score > 19 indicates homology		 VDPVNFKLLSQCIQVVLAAHMGK + 2 Deamidated (NQ); Oxidation (M)
667 386.7152 771.4158 771.4126 4.15 0 11 1 +1Score > 41 indicates identity
Score > 23 indicates homology		 QLAPQSK + Deamidated (NQ)
2478 527.9566 1580.8480 1580.8774 -18.6 1 11 0.4 +1Score > 38 indicates identity
Score > 19 indicates homology		 ISENQGVPVLIVANK + Deamidated (NQ)
2606 818.9597 1635.9048 1635.9243 -11.9 1 11 0.44 +1Score > 36 indicates identity
Score > 20 indicates homology		 QALPRMNILVATPGR
3958 845.4878 2533.4416 2533.4681 -10.5 1 11 0.14 +1Score > 29 indicates identity
Score > 15 indicates homology		 WPPFLLGCTILVILLVMKHVGK
4074 901.2055 2700.5947 2700.6306 -13.3 0 11 1 +1Score > 23 indicates identity
Score > 23 indicates homology		 VLSTPPVFLLGLLLALPLGAQGLSGVR
2997 618.9754 1853.9044 1853.9371 -17.6 0 11 0.49 +1Score > 40 indicates identity
Score > 20 indicates homology		 LQTLIVTHNQLDSVDR + 3 Deamidated (NQ)
3446 1071.5746 2141.1346 2141.1341 0.24 1 11 0.38 +1Score > 37 indicates identity
Score > 19 indicates homology		 VNNIQNGGIYIQATRGVAPR + Deamidated (NQ)
1639 593.3242 1184.6338 1184.6289 4.21 0 11 0.76 +1Score > 40 indicates identity
Score > 22 indicates homology		 TLSLPVDAQIE
2040 457.9160 1370.7262 1370.7266 -0.32 1 11 0.4 +1Score > 40 indicates identity
Score > 19 indicates homology		 EGRPSTNATLVAR
3159 396.4127 1977.0271 1977.0176 4.83 0 11 0.47 +1Score > 39 indicates identity
Score > 20 indicates homology		 NNGQAIHPTPMVGMVGLIK + Deamidated (NQ)
2879 593.6550 1777.9432 1777.9508 -4.31 1 11 0.31 +1Score > 38 indicates identity
Score > 18 indicates homology		 LRALSSLFSMLNQAAR + Deamidated (NQ)
3040 938.9546 1875.8946 1875.8950 -0.17 1 11 0.8 +1Score > 40 indicates identity
Score > 22 indicates homology		 QIEVTTDGAGVSGTVVDVE + Deamidated (NQ)
3549 731.7301 2192.1685 2192.1260 19.4 1 11 0.39 +1Score > 37 indicates identity
Score > 19 indicates homology		 LAGVGNGPPGTMQGTKLPVEPR + Deamidated (NQ); Oxidation (M)
366 316.1868 630.3590 630.3489 16.0 0 11 0.82 +1Score > 44 indicates identity
Score > 22 indicates homology		 AGGWLK
2407 776.3936 1550.7726 1550.8014 -18.5 1 11 0.97 +1Score > 41 indicates identity
Score > 23 indicates homology		 AIVTAENLLQLMGY + Oxidation (M)
2528 804.8959 1607.7772 1607.7573 12.4 1 11 0.76 +1Score > 41 indicates identity
Score > 22 indicates homology		 ALGTTMDRNTSNGIR + 2 Deamidated (NQ)
997 305.8321 914.4745 914.4610 14.7 0 11 0.83 +1Score > 41 indicates identity
Score > 22 indicates homology		 DIHNFIR + Deamidated (NQ)
428 336.1901 670.3656 670.3650 1.00 0 11 0.8 +1Score > 37 indicates identity
Score > 22 indicates homology		 AGDIAPK
2972 613.3392 1836.9958 1836.9622 18.3 0 11 0.71 +1Score > 37 indicates identity
Score > 22 indicates homology		 ASPPFPLIANVQDLVQE
1313 344.5041 1030.4905 1030.5043 -13.4 1 11 0.97 +1Score > 41 indicates identity
Score > 23 indicates homology		 GASQLVGENR + Deamidated (NQ)
1811 422.9202 1265.7388 1265.7231 12.4 1 11 0.81 +1Score > 34 indicates identity
Score > 22 indicates homology		 LPISLPNGVEVK + Deamidated (NQ)
3078 635.0309 1902.0709 1902.0364 18.1 0 11 0.11 +1Score > 33 indicates identity
Score > 13 indicates homology		 FGQVVGTGNLADFIPVLR
3391 1058.5374 2115.0602 2115.0275 15.5 1 11 0.48 +1Score > 39 indicates identity
Score > 20 indicates homology		 LYPTMIRQTGLGMGSTMAR + 2 Oxidation (M)
2563 539.2896 1614.8470 1614.8154 19.5 1 11 0.92 +1Score > 40 indicates identity
Score > 23 indicates homology		 RGGGGAGALLWQVFPE + Deamidated (NQ)
3109 642.3212 1923.9418 1923.9361 2.97 1 11 0.12 +1Score > 40 indicates identity
Score > 14 indicates homology		 DGTVRYDMTDLPVTAVR + Oxidation (M)
3801 1190.6470 2379.2794 2379.2885 -3.82 0 11 0.77 +1Score > 35 indicates identity
Score > 22 indicates homology		 FGIGADGVLFLAKPLHTSLHMR
387 647.3014 646.2941 646.3035 -14.4 0 11 0.84 +1Score > 42 indicates identity
Score > 22 indicates homology		 SGDNVR
2122 469.9077 1406.7013 1406.7041 -2.04 1 11 0.74 +1Score > 41 indicates identity
Score > 22 indicates homology		 LTLIADDFSEGAR
2161 476.2710 1425.7912 1425.7827 5.92 0 11 0.88 +1Score > 38 indicates identity
Score > 23 indicates homology		 LNLQAVLQGGALTE
3558 733.3651 2197.0735 2197.0434 13.7 0 11 0.67 +1Score > 39 indicates identity
Score > 21 indicates homology		 DHTKPVQQVLADMTNGGVDR + Deamidated (NQ); Oxidation (M)
2101 467.5787 1399.7143 1399.7195 -3.70 0 11 0.94 +1Score > 41 indicates identity
Score > 23 indicates homology		 QAVTNPNVVQSIK + 3 Deamidated (NQ)
3038 469.5210 1874.0549 1874.0262 15.3 1 11 0.61 +1Score > 33 indicates identity
Score > 21 indicates homology		 IRTNPTISLDLDFITR
3258 678.3530 2032.0372 2032.0034 16.6 1 11 0.24 +1Score > 39 indicates identity
Score > 17 indicates homology		 GQITKAVADMALNLLDVDE + Deamidated (NQ); Oxidation (M)
3438 712.7126 2135.1160 2135.0833 15.3 1 11 0.3 +1Score > 38 indicates identity
Score > 18 indicates homology		 LVHHDIIDALENMSRPFK + Deamidated (NQ)
3165 660.9836 1979.9290 1979.9225 3.29 1 10 0.33 +1Score > 39 indicates identity
Score > 18 indicates homology		 NYDYAVVNDEIPNAVQR + Deamidated (NQ)
4154 929.5129 2785.5169 2785.5059 3.95 0 10 1 +1Score > 32 indicates identity
Score > 23 indicates homology		 MLPVLFVTFVPSMMLAQIMTLFIK + Oxidation (M)
777 413.7442 825.4738 825.4821 -9.96 1 10 0.58 +1Score > 37 indicates identity
Score > 21 indicates homology		 LPGQRVR + Deamidated (NQ)
1882 433.2501 1296.7285 1296.7513 -17.6 1 10 0.87 +1Score > 38 indicates identity
Score > 22 indicates homology		 DAAKLANLNVLR
1697 405.2045 1212.5917 1212.6098 -15.0 1 10 0.57 +1Score > 40 indicates identity
Score > 21 indicates homology		 QEPQLELSNR
3056 943.0078 1884.0010 1883.9999 0.59 1 10 0.41 +1Score > 38 indicates identity
Score > 19 indicates homology		 NQVNQDLMIAGIRSGLR
1529 378.8714 1133.5924 1133.5863 5.38 1 10 0.28 +1Score > 42 indicates identity
Score > 17 indicates homology		 NMLLTGGRQK + Deamidated (NQ); Oxidation (M)
1592 388.2099 1161.6079 1161.6176 -8.36 0 10 0.89 +1Score > 41 indicates identity
Score > 22 indicates homology		 LMIGGTANLTR + Oxidation (M)
2518 535.9791 1604.9155 1604.9038 7.26 1 10 0.33 +1Score > 34 indicates identity
Score > 18 indicates homology		 QYLWSKNITAIIR
2232 735.3729 1468.7312 1468.7158 10.5 0 10 0.68 +1Score > 40 indicates identity
Score > 21 indicates homology		 APQGSQTHQVSLSK + 2 Deamidated (NQ)
2111 351.9375 1403.7209 1403.7296 -6.21 1 10 0.69 +1Score > 41 indicates identity
Score > 21 indicates homology		 ISTPIPRLSYQE + Deamidated (NQ)
2892 597.3118 1788.9136 1788.8979 8.75 1 10 0.93 +1Score > 40 indicates identity
Score > 23 indicates homology		 RPVTGNNVSHAHNKTR + 2 Deamidated (NQ)
3045 627.3406 1879.0000 1878.9772 12.1 1 10 0.66 +1Score > 38 indicates identity
Score > 21 indicates homology		 ALIASSNNAHIRGNQASR
4064 898.4242 2692.2508 2692.2843 -12.5 0 10 1 +1Score > 37 indicates identity
Score > 23 indicates homology		 TGLGFFDHMLDQIATHGGFQLQLK + 3 Deamidated (NQ); Oxidation (M)
2654 831.9543 1661.8940 1661.8923 1.06 1 10 0.33 +1Score > 38 indicates identity
Score > 18 indicates homology		 SRSLCNIPFITINK
2910 601.3326 1800.9760 1800.9556 11.3 1 10 0.86 +1Score > 38 indicates identity
Score > 22 indicates homology		 IQLRNPFANMGAQLVK + 2 Deamidated (NQ)
3209 668.3560 2002.0462 2002.0305 7.81 1 10 0.48 +1Score > 39 indicates identity
Score > 20 indicates homology		 ALMAARNQFLQLASQNPK + 2 Deamidated (NQ)
3738 772.3867 2314.1383 2314.1225 6.82 1 10 0.25 +1Score > 39 indicates identity
Score > 17 indicates homology		 MNNYETVFILTPVLSDAQMK + Deamidated (NQ)
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