MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 3643)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
463 341.6985 681.3824 681.3810 2.20 0 12 0.74 +1Score > 37 indicates identity
Score > 23 indicates homology
INIGHK + Deamidated (NQ)
1549 571.8622 1141.7098 1141.7070 2.45 0 12 0.96 +1Score > 30 indicates identity
Score > 24 indicates homology
ISIQVLQTLK
2537 537.9890 1610.9452 1610.9243 12.9 1 12 0.2 +1Score > 32 indicates identity
Score > 18 indicates homology
ILDGLAGKSVLVIGAGE
2666 834.4442 1666.8738 1666.8640 5.92 0 12 0.42 +1Score > 39 indicates identity
Score > 21 indicates homology
LNYLLLMVPNIPHE + 2 Deamidated (NQ)
1681 602.3478 1202.6810 1202.6758 4.38 1 12 0.88 +1Score > 38 indicates identity
Score > 24 indicates homology
IIKQTSALLSE + Deamidated (NQ)
2493 531.2815 1590.8227 1590.7923 19.1 1 12 0.97 +1Score > 40 indicates identity
Score > 24 indicates homology
DILSEAGTMQLLQR + Deamidated (NQ); Oxidation (M)
3474 1077.1163 2152.2180 2152.2038 6.61 1 12 0.15 +1Score > 32 indicates identity
Score > 16 indicates homology
LPGDISGVKPLGLMDITVRR + Oxidation (M)
1627 393.8893 1178.6461 1178.6336 10.6 0 12 0.44 +1Score > 38 indicates identity
Score > 21 indicates homology
AGVFDAYVLPK
4310 998.4979 2992.4719 2992.5150 -14.4 0 12 0.2 +1Score > 37 indicates identity
Score > 18 indicates homology
ALCAQTMLVSHPGVNVLLLDDGLQHYK + Deamidated (NQ)
2314 755.3873 1508.7600 1508.7511 5.90 0 12 0.43 +1Score > 40 indicates identity
Score > 21 indicates homology
VGAVDGDIVFFGADK
3437 712.7125 2135.1157 2135.0833 15.1 1 12 0.23 +1Score > 38 indicates identity
Score > 18 indicates homology
LVHHDIIDALENMSRPFK + Deamidated (NQ)
1043 464.7123 927.4100 927.3974 13.6 1 12 0.99 +1Score > 38 indicates identity
Score > 24 indicates homology
EFFDQNK + Deamidated (NQ)
1430 363.2060 1086.5962 1086.6033 -6.58 0 12 0.81 +1Score > 41 indicates identity
Score > 23 indicates homology
SAIVDVLTNR
3304 686.3409 2056.0009 2055.9758 12.2 1 12 0.63 +1Score > 40 indicates identity
Score > 22 indicates homology
VMVYQDGAEVQFTGMLPR + Oxidation (M)
1423 362.5462 1084.6168 1084.6142 2.42 1 12 0.9 +1Score > 39 indicates identity
Score > 24 indicates homology
QGAKGLAWVR
1060 466.2484 930.4822 930.4923 -10.8 0 12 0.92 +1Score > 42 indicates identity
Score > 24 indicates homology
INPAGGFVR + Deamidated (NQ)
1445 549.2757 1096.5368 1096.5440 -6.56 1 12 0.73 +1Score > 40 indicates identity
Score > 23 indicates homology
LYGYKLNPE + Deamidated (NQ)
1632 591.3683 1180.7220 1180.7067 13.0 1 12 0.85 +1Score > 31 indicates identity
Score > 24 indicates homology
DVVPVGILLKE
301 300.7087 599.4028 599.4006 3.69 0 12 0.97 +1Score > 40 indicates identity
Score > 24 indicates homology
AIGLVK
4077 676.6376 2702.5213 2702.4690 19.3 1 12 1 +1Score > 29 indicates identity
Score > 24 indicates homology
GVIVRPVANYGMPNHLRITIGLPAE + Oxidation (M)
1223 332.8550 995.5432 995.5512 -8.07 0 12 0.89 +1Score > 37 indicates identity
Score > 24 indicates homology
NVSPGIRPR + Deamidated (NQ)
2423 520.2935 1557.8587 1557.8879 -18.7 1 12 0.24 +1Score > 37 indicates identity
Score > 18 indicates homology
LVGGVALDPLYKTGR
3743 1160.0636 2318.1126 2318.1543 -18.0 1 12 0.78 +1Score > 39 indicates identity
Score > 23 indicates homology
IGQSVNIISDLYGENVVFHGR + 2 Deamidated (NQ)
861 428.7316 855.4486 855.4450 4.26 0 12 0.95 +1Score > 39 indicates identity
Score > 24 indicates homology
IGNQPLGR + 2 Deamidated (NQ)
3581 737.3940 2209.1602 2209.1729 -5.77 1 12 0.53 +1Score > 37 indicates identity
Score > 21 indicates homology
LLQIEDDNGGILAIPAVDTIK + 2 Deamidated (NQ)
3890 822.4554 2464.3444 2464.3550 -4.32 1 12 0.12 +1Score > 34 indicates identity
Score > 15 indicates homology
QLRPYVIDASLLIDQAVRTHR + Deamidated (NQ)
1444 548.3364 1094.6582 1094.6560 2.06 1 12 0.96 +1Score > 32 indicates identity
Score > 24 indicates homology
ARVALPQALR + Deamidated (NQ)
1975 672.3678 1342.7210 1342.7278 -5.06 1 12 0.77 +1Score > 40 indicates identity
Score > 23 indicates homology
KQVLMITSSAHK + Deamidated (NQ)
1025 308.5164 922.5274 922.5097 19.2 1 12 0.44 +1Score > 37 indicates identity
Score > 21 indicates homology
RHINNIR + Deamidated (NQ)
2999 928.4593 1854.9040 1854.9224 -9.89 0 12 0.65 +1Score > 40 indicates identity
Score > 22 indicates homology
VPAPYGTGPAAAAQAASAAGR + Deamidated (NQ)
2667 834.4485 1666.8824 1666.8890 -3.92 1 12 0.54 +1Score > 39 indicates identity
Score > 22 indicates homology
SIVGEIVQNLINDPR + Deamidated (NQ)
3207 1001.0352 2000.0558 2000.0765 -10.3 1 12 0.79 +1Score > 38 indicates identity
Score > 23 indicates homology
LVITPQDAEMVVGFLVNR
3285 681.6791 2042.0155 2042.0354 -9.75 1 12 0.74 +1Score > 39 indicates identity
Score > 23 indicates homology
TMAAILENGVQADGSVVLPR + 2 Deamidated (NQ)
699 392.7570 783.4994 783.5079 -10.8 1 12 0.93 +1Score > 36 indicates identity
Score > 24 indicates homology
VIAAVRR
1978 448.9036 1343.6890 1343.6932 -3.16 0 12 0.96 +1Score > 41 indicates identity
Score > 24 indicates homology
LINDSINLINGSA + Deamidated (NQ)
2698 558.6526 1672.9360 1672.9261 5.93 1 12 0.45 +1Score > 36 indicates identity
Score > 21 indicates homology
VKTSVGVWGALLNSSR
2863 442.9535 1767.7849 1767.7660 10.7 1 12 0.41 +1Score > 39 indicates identity
Score > 20 indicates homology
SQSRSYSSADSYSSTR
3715 769.0380 2304.0922 2304.1170 -10.8 1 12 0.26 +1Score > 39 indicates identity
Score > 18 indicates homology
VFMGDFPLMTTKGTFIINGAE + Oxidation (M)
3102 480.4933 1917.9441 1917.9102 17.7 0 12 0.72 +1Score > 40 indicates identity
Score > 23 indicates homology
AGLQDNDLAVSLNGMDLR + Deamidated (NQ); Oxidation (M)
3599 558.0048 2227.9901 2228.0055 -6.93 1 12 0.084 +1Score > 37 indicates identity
Score > 13 indicates homology
SPYHDACPATQQPSVIQAEK + 2 Deamidated (NQ)
3527 546.5497 2182.1697 2182.1568 5.90 1 12 0.27 +1Score > 36 indicates identity
Score > 18 indicates homology
DIRVIVVGNPANTNAWIAMK + Deamidated (NQ)
1554 573.8190 1145.6234 1145.6115 10.5 0 12 0.96 +1Score > 40 indicates identity
Score > 24 indicates homology
LTDPSVMVLR + Oxidation (M)
3381 1056.0520 2110.0894 2110.0946 -2.45 1 12 0.35 +1Score > 38 indicates identity
Score > 20 indicates homology
AGQDIQEVIDNLPINVPFK + Deamidated (NQ)
2118 703.8953 1405.7760 1405.7790 -2.07 1 12 0.66 +1Score > 38 indicates identity
Score > 22 indicates homology
APARGAAPVNNLQK
1622 589.3010 1176.5874 1176.5809 5.60 1 12 0.99 +1Score > 42 indicates identity
Score > 24 indicates homology
RVQLSTALCE + Deamidated (NQ)
2523 803.9445 1605.8744 1605.8614 8.15 1 12 0.49 +1Score > 37 indicates identity
Score > 21 indicates homology
IVYDNSIGITLELR + Deamidated (NQ)
2716 846.8739 1691.7332 1691.7355 -1.34 0 12 0.36 +1Score > 37 indicates identity
Score > 20 indicates homology
NGANAGMIVGSCQNAAR + 2 Deamidated (NQ)
1266 337.5271 1009.5595 1009.5597 -0.19 0 12 0.96 +1Score > 37 indicates identity
Score > 24 indicates homology
FFSLDILR
1784 414.8912 1241.6518 1241.6728 -16.9 1 12 0.73 +1Score > 40 indicates identity
Score > 23 indicates homology
NLDGKNLALQR + Deamidated (NQ)
1942 664.3890 1326.7634 1326.7659 -1.88 1 12 0.24 +1Score > 37 indicates identity
Score > 18 indicates homology
ISLDHLIFNKK
3024 933.5029 1864.9912 1864.9829 4.48 1 12 0.49 +1Score > 38 indicates identity
Score > 21 indicates homology
GMKVPDVLLSGNHAAIAR + Deamidated (NQ); Oxidation (M)
1191 327.5194 979.5364 979.5412 -4.97 1 12 0.42 +1Score > 38 indicates identity
Score > 20 indicates homology
EFVVMVLK + Oxidation (M)
2717 846.8766 1691.7386 1691.7355 1.85 0 12 0.3 +1Score > 38 indicates identity
Score > 19 indicates homology
NGANAGMIVGSCQNAAR + 2 Deamidated (NQ)
3047 627.6755 1880.0047 1880.0255 -11.1 0 12 0.29 +1Score > 38 indicates identity
Score > 19 indicates homology
AISDAVTIPVIASGGVGEPK
2875 592.6713 1774.9921 1775.0094 -9.74 1 11 0.57 +1Score > 35 indicates identity
Score > 22 indicates homology
YWGIKLATNAAVTVLR
1899 655.3302 1308.6458 1308.6422 2.79 1 11 0.87 +1Score > 42 indicates identity
Score > 23 indicates homology
KSANNAHQVPDK + Deamidated (NQ)
2117 703.8951 1405.7756 1405.7790 -2.36 1 11 0.6 +1Score > 37 indicates identity
Score > 22 indicates homology
APARGAAPVNNLQK
2495 531.6157 1591.8253 1591.8107 9.15 1 11 0.85 +1Score > 40 indicates identity
Score > 23 indicates homology
WYTVSVRGPDGSIR
1277 506.7419 1011.4692 1011.4734 -4.08 1 11 0.86 +1Score > 40 indicates identity
Score > 23 indicates homology
HVDTAQEGR
2129 471.5983 1411.7731 1411.7823 -6.57 1 11 0.33 +1Score > 38 indicates identity
Score > 19 indicates homology
QIVVNVHFSIEK
2719 423.9814 1691.8965 1691.8730 13.9 1 11 0.77 +1Score > 39 indicates identity
Score > 23 indicates homology
ASQQIQDLRILYDK + 2 Deamidated (NQ)
3687 765.4287 2293.2643 2293.2529 4.95 1 11 0.15 +1Score > 33 indicates identity
Score > 16 indicates homology
KPIEVPAGVTVNVDSNNLVTVK + Deamidated (NQ)
513 351.7118 701.4090 701.4072 2.66 0 11 0.96 +1Score > 43 indicates identity
Score > 24 indicates homology
IIDSVR
2992 925.4537 1848.8928 1848.9026 -5.29 1 11 0.18 +1Score > 40 indicates identity
Score > 17 indicates homology
GQLSSVTAVLQEMNLLE + 2 Deamidated (NQ); Oxidation (M)
1046 464.7142 927.4138 927.4225 -9.36 1 11 0.63 +1Score > 38 indicates identity
Score > 22 indicates homology
YIFNEIE + Deamidated (NQ)
638 380.7245 759.4344 759.4239 13.9 1 11 0.98 +1Score > 41 indicates identity
Score > 24 indicates homology
ASRADIK
1963 669.3650 1336.7154 1336.6948 15.4 1 11 0.2 +1Score > 39 indicates identity
Score > 17 indicates homology
VLGLTVAEMLFE + Oxidation (M)
2319 378.7176 1510.8413 1510.8157 16.9 1 11 0.57 +1Score > 37 indicates identity
Score > 21 indicates homology
LFWLAHATSPRGR
2791 864.4564 1726.8982 1726.9326 -19.9 1 11 0.57 +1Score > 39 indicates identity
Score > 21 indicates homology
RIIQLNGGTVDSALNR + Deamidated (NQ)
3466 717.0226 2148.0460 2148.0853 -18.3 1 11 0.33 +1Score > 39 indicates identity
Score > 19 indicates homology
SPQAQKTFIYLLLAFFME + 2 Deamidated (NQ)
3067 631.6622 1891.9648 1891.9826 -9.41 1 11 0.66 +1Score > 40 indicates identity
Score > 22 indicates homology
IALTTAMRDSGTVLNWK + Oxidation (M)
2963 609.6713 1825.9921 1826.0261 -18.6 1 11 0.17 +1Score > 37 indicates identity
Score > 16 indicates homology
LNSRPLSSNLLDKLQK + Deamidated (NQ)
532 354.7065 707.3984 707.3966 2.60 0 11 0.63 +1Score > 39 indicates identity
Score > 22 indicates homology
LSAYVR
1295 510.3028 1018.5910 1018.5845 6.43 0 11 0.95 +1Score > 37 indicates identity
Score > 24 indicates homology
ITVVTAMLR + Oxidation (M)
3648 752.3780 2254.1122 2254.0827 13.1 1 11 0.88 +1Score > 39 indicates identity
Score > 23 indicates homology
DGIQVDMIFYKGNAISVSAPE + Deamidated (NQ)
1217 497.2756 992.5366 992.5291 7.62 1 11 0.31 +1Score > 41 indicates identity
Score > 19 indicates homology
ASIAKQFQT
3183 663.3182 1986.9328 1986.9534 -10.4 1 11 0.42 +1Score > 39 indicates identity
Score > 20 indicates homology
FSHINQPVKTLSGGNQQK + 5 Deamidated (NQ)
1424 362.5463 1084.6171 1084.6063 9.97 1 11 0.96 +1Score > 39 indicates identity
Score > 24 indicates homology
RPIMGAIAEK
1920 660.3876 1318.7606 1318.7497 8.33 1 11 0.84 +1Score > 35 indicates identity
Score > 23 indicates homology
FLGTTKDPVTLK
2477 791.4199 1580.8252 1580.7988 16.8 0 11 0.1 +1Score > 39 indicates identity
Score > 14 indicates homology
TSVGTFGSGWGWLVK
2720 565.2856 1692.8350 1692.8683 -19.7 0 11 0.85 +1Score > 41 indicates identity
Score > 23 indicates homology
AVLGSNHVQLQLAVDE + Deamidated (NQ)
1585 580.3618 1158.7090 1158.7046 3.85 1 11 0.32 +1Score > 31 indicates identity
Score > 19 indicates homology
SLPLLMITKK + Oxidation (M)
2898 598.3012 1791.8818 1791.8686 7.36 1 11 0.8 +1Score > 41 indicates identity
Score > 23 indicates homology
MNWAISNLTGGNRVSR + Deamidated (NQ); Oxidation (M)
797 417.2213 832.4280 832.4290 -1.16 1 11 0.96 +1Score > 43 indicates identity
Score > 24 indicates homology
KADNVASK + Deamidated (NQ)
1993 451.6163 1351.8271 1351.8122 11.0 1 11 0.3 +1Score > 29 indicates identity
Score > 19 indicates homology
RIAQMVIAPVVR
3192 996.0232 1990.0318 1990.0081 11.9 1 11 0.88 +1Score > 39 indicates identity
Score > 23 indicates homology
QTTEPNYPEMLLIAINK + Oxidation (M)
1537 380.2007 1137.5803 1137.5891 -7.73 1 11 0.65 +1Score > 41 indicates identity
Score > 22 indicates homology
VSYGAGTRTAR
2289 500.5884 1498.7434 1498.7627 -12.9 1 11 0.95 +1Score > 40 indicates identity
Score > 24 indicates homology
VANGQVVAELIDDR + Deamidated (NQ)
4202 948.5524 2842.6354 2842.5891 16.3 1 11 1 +1Score > 26 indicates identity
Score > 24 indicates homology
IIITWSRGSTIIPIMIGHTIAIHNGK + Deamidated (NQ)
3699 1150.0905 2298.1664 2298.1300 15.9 1 11 0.5 +1Score > 38 indicates identity
Score > 21 indicates homology
NPENLINDAPIKPTLIGMSNK + 4 Deamidated (NQ); Oxidation (M)
3193 996.0238 1990.0330 1990.0081 12.5 1 11 0.54 +1Score > 39 indicates identity
Score > 21 indicates homology
QTTEPNYPEMLLIAINK + Oxidation (M)
2725 566.3143 1695.9211 1695.9056 9.11 1 11 0.55 +1Score > 38 indicates identity
Score > 21 indicates homology
IFQVNGHNRLLVQR + 3 Deamidated (NQ)
3195 664.3538 1990.0396 1990.0007 19.5 1 11 0.18 +1Score > 39 indicates identity
Score > 16 indicates homology
LSYNNIQDGNAALQIVKE + Deamidated (NQ)
3266 679.3924 2035.1554 2035.1605 -2.52 1 11 0.32 +1Score > 31 indicates identity
Score > 19 indicates homology
VQLVLPLLVAEAAAAPAFLE + Deamidated (NQ)
3383 704.3723 2110.0951 2110.0921 1.41 1 11 0.32 +1Score > 38 indicates identity
Score > 19 indicates homology
LLFNDIIFRMPNPNYVK + Deamidated (NQ); Oxidation (M)
3713 768.3845 2302.1317 2302.1176 6.11 1 11 0.95 +1Score > 39 indicates identity
Score > 23 indicates homology
SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ)
1822 424.5706 1270.6900 1270.6670 18.1 0 11 0.88 +1Score > 40 indicates identity
Score > 23 indicates homology
ASAPPPPPPPSTR
1922 440.9041 1319.6905 1319.7085 -13.7 0 11 0.46 +1Score > 41 indicates identity
Score > 20 indicates homology
ANALFLDVLTSR + Deamidated (NQ)
3802 1190.6472 2379.2798 2379.2885 -3.65 0 11 0.92 +1Score > 35 indicates identity
Score > 23 indicates homology
FGIGADGVLFLAKPLHTSLHMR
2017 682.3682 1362.7218 1362.7183 2.59 1 11 0.41 +1Score > 40 indicates identity
Score > 20 indicates homology
WILAEFNQTIK + Deamidated (NQ)
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