MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2594 408.7240 1630.8669 1630.8678 -0.57 0 13 0.47 +1Score > 39 indicates identity
Score > 22 indicates homology		 INDGVAIALYGLNAAR + Deamidated (NQ)
3176 662.0162 1983.0268 1983.0023 12.3 1 13 0.76 +1Score > 39 indicates identity
Score > 24 indicates homology		 LYVYRLPDQQLMSVIE + Deamidated (NQ); Oxidation (M)
3936 837.4659 2509.3759 2509.3462 11.8 1 13 0.11 +1Score > 33 indicates identity
Score > 16 indicates homology		 VAEMLAPVLTGNDLILVQGAGNIGK + Deamidated (NQ); Oxidation (M)
3083 954.4214 1906.8282 1906.8513 -12.1 1 13 0.3 +1Score > 36 indicates identity
Score > 20 indicates homology		 IENAIMQGLQQCSSGGGR + 2 Deamidated (NQ)
1915 439.8835 1316.6287 1316.6217 5.32 0 13 0.85 +1Score > 41 indicates identity
Score > 25 indicates homology		 DLLGTNMMLHR + Deamidated (NQ); Oxidation (M)
413 333.6779 665.3412 665.3384 4.27 0 13 0.89 +1Score > 41 indicates identity
Score > 25 indicates homology		 NAYGLK + Deamidated (NQ)
1326 518.2974 1034.5802 1034.5760 4.10 1 13 0.97 +1Score > 38 indicates identity
Score > 25 indicates homology		 IFAKNNISK + Deamidated (NQ)
2551 404.2327 1612.9017 1612.9162 -8.96 1 13 0.69 +1Score > 37 indicates identity
Score > 24 indicates homology		 TIVRSDPHIGLLHR
566 364.2183 726.4220 726.4276 -7.64 0 13 0.51 +1Score > 36 indicates identity
Score > 22 indicates homology		 GPVLVDK
640 381.1835 760.3524 760.3603 -10.3 1 13 0.79 +1Score > 41 indicates identity
Score > 24 indicates homology		 NEAQAVK + 2 Deamidated (NQ)
717 397.2050 792.3954 792.3878 9.63 1 13 0.93 +1Score > 42 indicates identity
Score > 25 indicates homology		 RNAQFR + 2 Deamidated (NQ)
2233 735.3730 1468.7314 1468.7449 -9.19 0 13 0.51 +1Score > 40 indicates identity
Score > 22 indicates homology		 SQLIDILDFDYK
2587 814.9686 1627.9226 1627.9046 11.1 1 13 0.97 +1Score > 35 indicates identity
Score > 25 indicates homology		 GLVHPSSQHKSIPLK + Deamidated (NQ)
2718 423.9729 1691.8625 1691.8400 13.3 0 13 0.75 +1Score > 40 indicates identity
Score > 24 indicates homology		 MATTTATTPPSLTDIR + Oxidation (M)
1140 480.7743 959.5340 959.5175 17.3 0 13 0.81 +1Score > 40 indicates identity
Score > 24 indicates homology		 LLSLANSIE + Deamidated (NQ)
1128 319.8390 956.4952 956.5039 -9.16 0 13 0.89 +1Score > 39 indicates identity
Score > 25 indicates homology		 TLASSVHSR
1674 600.3745 1198.7344 1198.7285 4.96 1 13 0.96 +1Score > 32 indicates identity
Score > 25 indicates homology		 RTLQLVDIIK + Deamidated (NQ)
2865 886.9628 1771.9110 1771.9005 5.93 0 13 0.39 +1Score > 40 indicates identity
Score > 21 indicates homology		 IFQLTEPGNRPASWR + Deamidated (NQ)
3389 705.3886 2113.1440 2113.1380 2.81 0 13 0.15 +1Score > 36 indicates identity
Score > 17 indicates homology		 WNQLDLAIVLLSIMGIALE + 2 Deamidated (NQ)
2095 466.2607 1395.7603 1395.7650 -3.35 1 13 0.21 +1Score > 38 indicates identity
Score > 19 indicates homology		 LDGYDIISLIKF
1956 445.2663 1332.7771 1332.7613 11.8 1 13 0.32 +1Score > 33 indicates identity
Score > 20 indicates homology		 VVALTGSSGKTSVK
1037 464.2736 926.5326 926.5297 3.14 1 13 0.86 +1Score > 38 indicates identity
Score > 25 indicates homology		 KVLSNPNR
449 337.6966 673.3786 673.3759 4.13 1 13 0.38 +1Score > 42 indicates identity
Score > 21 indicates homology		 IESAVR
2221 487.5938 1459.7596 1459.7493 7.03 0 13 0.62 +1Score > 41 indicates identity
Score > 23 indicates homology		 NINVSAATPLWMK + Oxidation (M)
3557 550.2756 2197.0733 2197.0592 6.40 1 13 0.83 +1Score > 39 indicates identity
Score > 24 indicates homology		 ASEQFGWDINYGATALLWR
1358 527.3091 1052.6036 1052.5978 5.53 1 13 0.32 +1Score > 36 indicates identity
Score > 20 indicates homology		 IVVPLNNRE
3393 1058.5422 2115.0698 2115.1060 -17.1 1 13 0.6 +1Score > 39 indicates identity
Score > 23 indicates homology		 VTTITGTNGQPTDETVIVIR + Deamidated (NQ)
639 380.7349 759.4552 759.4490 8.18 1 13 0.96 +1Score > 37 indicates identity
Score > 25 indicates homology		 LGTKNVK + Deamidated (NQ)
2549 538.6261 1612.8565 1612.8420 8.97 1 13 0.54 +1Score > 39 indicates identity
Score > 23 indicates homology		 LSARPGNEVNLLDSK + Deamidated (NQ)
1085 314.8369 941.4889 941.4753 14.5 0 13 0.99 +1Score > 40 indicates identity
Score > 25 indicates homology		 HQAMLAVR + Deamidated (NQ); Oxidation (M)
1548 571.8621 1141.7096 1141.7182 -7.53 1 13 0.99 +1Score > 30 indicates identity
Score > 25 indicates homology		 LSLRAASALIK
1609 391.5696 1171.6870 1171.6812 4.90 0 13 0.76 +1Score > 36 indicates identity
Score > 24 indicates homology		 ITQVTDLILR + Deamidated (NQ)
2435 782.3906 1562.7666 1562.7438 14.6 1 13 0.82 +1Score > 41 indicates identity
Score > 24 indicates homology		 LTGVRDVTDHQHGE
2800 577.3261 1728.9565 1728.9523 2.44 1 13 0.86 +1Score > 37 indicates identity
Score > 25 indicates homology		 GTLNVRLSFKPPADSK
3721 1154.0570 2306.0994 2306.0962 1.39 1 13 0.24 +1Score > 39 indicates identity
Score > 19 indicates homology		 MNNLIKAYAAGVMSAAFLFGSE + 2 Deamidated (NQ)
2527 804.8954 1607.7762 1607.7573 11.8 1 13 0.85 +1Score > 41 indicates identity
Score > 24 indicates homology		 ALGTTMDRNTSNGIR + 2 Deamidated (NQ)
1778 413.9049 1238.6929 1238.7095 -13.4 0 13 0.4 +1Score > 38 indicates identity
Score > 21 indicates homology		 LAAAQAALNQLR
2024 683.8419 1365.6692 1365.6524 12.3 1 13 0.46 +1Score > 41 indicates identity
Score > 22 indicates homology		 TANATALGYEQAR + Deamidated (NQ)
312 301.6983 601.3820 601.3799 3.55 0 13 0.37 +1Score > 41 indicates identity
Score > 21 indicates homology		 SLVVGK
378 322.2105 642.4064 642.4177 -17.5 1 13 0.92 +1Score > 42 indicates identity
Score > 25 indicates homology		 KGIVAR
1069 468.7660 935.5174 935.4998 18.9 1 13 0.7 +1Score > 38 indicates identity
Score > 24 indicates homology		 TTMITKLE
2769 857.4453 1712.8760 1712.8886 -7.33 0 13 0.61 +1Score > 40 indicates identity
Score > 23 indicates homology		 APFVDIVLGPQTYHR + Deamidated (NQ)
1224 332.8550 995.5432 995.5400 3.22 1 13 0.84 +1Score > 37 indicates identity
Score > 24 indicates homology		 ASLAHAEGLK
1783 621.4034 1240.7922 1240.7754 13.5 1 13 0.85 +1Score > 24 indicates identity LKLSAAGIDLIK
2379 769.8945 1537.7744 1537.7698 3.04 1 13 0.47 +1Score > 40 indicates identity
Score > 22 indicates homology		 LVGTAIADFNMIEK + Deamidated (NQ); Oxidation (M)
3796 791.7905 2372.3497 2372.3290 8.71 1 13 0.6 +1Score > 30 indicates identity
Score > 23 indicates homology		 ILGFMVLKDTQVLVNVWAIGR + Deamidated (NQ)
3177 662.0173 1983.0301 1982.9983 16.0 0 13 0.59 +1Score > 39 indicates identity
Score > 23 indicates homology		 SMSSVVLASALSNNFIAQK + Deamidated (NQ); Oxidation (M)
1166 324.8391 971.4955 971.5036 -8.37 1 12 0.98 +1Score > 41 indicates identity
Score > 25 indicates homology		 QGIPTETAR
4213 956.1541 2865.4405 2865.4292 3.92 0 12 0.13 +1Score > 37 indicates identity
Score > 16 indicates homology		 MIDLFQLTCLTSTVSISNFILYNR + Deamidated (NQ); Oxidation (M)
1286 339.2165 1014.6277 1014.6226 5.02 1 12 0.55 +1Score > 31 indicates identity
Score > 22 indicates homology		 FLELKPLR
1340 521.7805 1041.5464 1041.5342 11.7 1 12 0.62 +1Score > 39 indicates identity
Score > 23 indicates homology		 VSLSPGPEQK + Deamidated (NQ)
3092 638.9912 1913.9518 1913.9418 5.22 1 12 0.38 +1Score > 40 indicates identity
Score > 21 indicates homology		 NWLMQGVAAQGDRVQLE
3225 671.3344 2010.9814 2010.9660 7.63 0 12 0.49 +1Score > 40 indicates identity
Score > 22 indicates homology		 QLTHQQIYPHFADGSNR
773 412.2028 822.3910 822.3984 -8.95 1 12 0.83 +1Score > 42 indicates identity
Score > 24 indicates homology		 GDPHELR
595 370.7043 739.3940 739.3977 -4.91 0 12 0.39 +1Score > 38 indicates identity
Score > 21 indicates homology		 AVANHTK
2627 549.6508 1645.9306 1645.9443 -8.34 0 12 0.25 +1Score > 35 indicates identity
Score > 19 indicates homology		 LYFGNLVPIIAQGLK + Deamidated (NQ)
583 366.2194 730.4242 730.4337 -13.0 0 12 0.66 +1Score > 43 indicates identity
Score > 23 indicates homology		 AISTAIR
3674 760.3990 2278.1752 2278.1416 14.7 1 12 0.16 +1Score > 38 indicates identity
Score > 17 indicates homology		 WGYGATGVVSVASNLVPGLMRE + Oxidation (M)
3668 758.7449 2273.2129 2273.1837 12.8 1 12 0.22 +1Score > 36 indicates identity
Score > 18 indicates homology		 KSNFSLAILNVGAPAAGMNAAVR + 2 Deamidated (NQ)
3723 1154.0577 2306.1008 2306.0962 2.00 1 12 0.76 +1Score > 39 indicates identity
Score > 24 indicates homology		 MNNLIKAYAAGVMSAAFLFGSE + 2 Deamidated (NQ)
1382 532.2776 1062.5406 1062.5458 -4.84 0 12 0.65 +1Score > 42 indicates identity
Score > 23 indicates homology		 ALQYGDIQR
2682 557.3370 1668.9892 1668.9563 19.7 1 12 0.23 +1Score > 30 indicates identity
Score > 19 indicates homology		 SRLPLLDLNIYVPR + Deamidated (NQ)
2787 863.5143 1725.0140 1724.9923 12.6 1 12 0.14 +1Score > 31 indicates identity
Score > 16 indicates homology		 LGNLLLVLSLANEINK + 2 Deamidated (NQ)
2876 593.3073 1776.9001 1776.9158 -8.88 1 12 0.68 +1Score > 40 indicates identity
Score > 23 indicates homology		 HINDYSIIYLVDRR + Deamidated (NQ)
752 811.4166 810.4093 810.4058 4.36 0 12 0.44 +1Score > 40 indicates identity
Score > 21 indicates homology		 GMLATYR
605 372.2570 742.4994 742.4953 5.63 0 12 0.81 +1Score > 32 indicates identity
Score > 24 indicates homology		 VLATVLK
3392 1058.5411 2115.0676 2115.0847 -8.08 1 12 0.79 +1Score > 39 indicates identity
Score > 24 indicates homology		 ALANDDLQLAENLLGKPYR + 2 Deamidated (NQ)
3511 725.3725 2173.0957 2173.0750 9.51 1 12 0.86 +1Score > 39 indicates identity
Score > 24 indicates homology		 TDSANNGDIVVALIDNEATLK + Deamidated (NQ)
3484 721.7211 2162.1415 2162.1214 9.27 1 12 0.31 +1Score > 37 indicates identity
Score > 20 indicates homology		 LISSSLCLLTDQVIEMVIE
2659 833.4913 1664.9680 1664.9423 15.5 0 12 0.77 +1Score > 32 indicates identity
Score > 24 indicates homology		 LIGPDIIMEPLVLDK
3799 1190.6439 2379.2732 2379.2885 -6.43 0 12 0.9 +1Score > 35 indicates identity
Score > 24 indicates homology		 FGIGADGVLFLAKPLHTSLHMR
1202 494.2655 986.5164 986.5033 13.4 1 12 0.84 +1Score > 42 indicates identity
Score > 24 indicates homology		 AGAQGQVEVK + Deamidated (NQ)
1849 427.5945 1279.7617 1279.7764 -11.5 1 12 0.81 +1Score > 33 indicates identity
Score > 24 indicates homology		 ILAWNKAPVLR
3344 696.0279 2085.0619 2085.0854 -11.3 1 12 0.47 +1Score > 39 indicates identity
Score > 22 indicates homology		 AQPGGLAALQAALADATVEYR
1496 374.5384 1120.5934 1120.5764 15.1 1 12 0.85 +1Score > 41 indicates identity
Score > 24 indicates homology		 GELYLNGINK + Deamidated (NQ)
1211 990.5159 989.5086 989.4964 12.4 0 12 0.73 +1Score > 42 indicates identity
Score > 23 indicates homology		 QDLSGLMAR
2540 806.9337 1611.8528 1611.8832 -18.8 0 12 0.73 +1Score > 39 indicates identity
Score > 23 indicates homology		 GLSVGDTIQAGVSAPIK
2760 855.4304 1708.8462 1708.8342 7.07 0 12 0.8 +1Score > 40 indicates identity
Score > 24 indicates homology		 FANAGVQAIICTDISK + 2 Deamidated (NQ)
3147 983.5087 1965.0028 1964.9990 1.98 1 12 0.74 +1Score > 39 indicates identity
Score > 23 indicates homology		 AGARAGMVAVDQTTIDYVK
3203 1000.0359 1998.0572 1998.0574 -0.078 1 12 0.64 +1Score > 38 indicates identity
Score > 23 indicates homology		 NIILQNPFNKIQIHFR + 4 Deamidated (NQ)
1351 349.8416 1046.5030 1046.5179 -14.2 1 12 0.66 +1Score > 41 indicates identity
Score > 23 indicates homology		 KGTPGDLMGR + Oxidation (M)
3242 1012.5286 2023.0426 2023.0698 -13.4 1 12 0.64 +1Score > 39 indicates identity
Score > 23 indicates homology		 SAALQSIQENLGLQDAPLR
1852 640.8922 1279.7698 1279.7864 -12.9 0 12 0.18 +1Score > 32 indicates identity
Score > 17 indicates homology		 ISGLSHITLIVK
2063 692.8244 1383.6342 1383.6227 8.32 1 12 0.27 +1Score > 40 indicates identity
Score > 19 indicates homology		 NAINENTAFLMK + 3 Deamidated (NQ); Oxidation (M)
1974 672.3675 1342.7204 1342.7278 -5.51 1 12 0.99 +1Score > 40 indicates identity
Score > 25 indicates homology		 KQVLMITSSAHK + Deamidated (NQ)
4010 866.7974 2597.3704 2597.4098 -15.2 1 12 0.092 +1Score > 36 indicates identity
Score > 14 indicates homology		 MLAQVLTGNDLILIQGAGNIGKIAR + 3 Deamidated (NQ); Oxidation (M)
3497 1086.1481 2170.2816 2170.2514 13.9 1 12 0.1 +1Score > 26 indicates identity
Score > 15 indicates homology		 ILNSGQFKPPVIVFVNIKR + 2 Deamidated (NQ)
3414 708.0742 2121.2008 2121.1649 16.9 0 12 0.11 +1Score > 31 indicates identity
Score > 15 indicates homology		 IDFIQVIIYIGFPNLLIE + 2 Deamidated (NQ)
3578 1105.1362 2208.2578 2208.2531 2.15 1 12 0.42 +1Score > 30 indicates identity
Score > 21 indicates homology		 NIINLEPVLNVLRWHLHK + Deamidated (NQ)
2010 453.5793 1357.7161 1357.7275 -8.42 1 12 0.38 +1Score > 40 indicates identity
Score > 20 indicates homology		 AMGLEAGLVGQLAK + Deamidated (NQ)
3264 1018.0433 2034.0720 2034.0860 -6.84 1 12 0.71 +1Score > 38 indicates identity
Score > 23 indicates homology		 AIFNQDILPFKDLIAMGK + Deamidated (NQ)
2056 690.3383 1378.6620 1378.6616 0.32 1 12 0.5 +1Score > 41 indicates identity
Score > 22 indicates homology		 DLISFDIDANEK
2761 570.9625 1709.8657 1709.8525 7.69 1 12 0.56 +1Score > 40 indicates identity
Score > 22 indicates homology		 DYIGRPNPLYFAER
1970 671.3813 1340.7480 1340.7551 -5.27 0 12 0.5 +1Score > 38 indicates identity
Score > 22 indicates homology		 LNPSLVISLSQGL + Deamidated (NQ)
2795 864.9787 1727.9428 1727.9682 -14.7 1 12 0.52 +1Score > 38 indicates identity
Score > 22 indicates homology		 RNVVSWNTLVTGLIR + Deamidated (NQ)
1159 485.7115 969.4084 969.4113 -2.99 0 12 0.97 +1Score > 38 indicates identity
Score > 24 indicates homology		 TMFDTNPK + Deamidated (NQ); Oxidation (M)
2521 803.9329 1605.8512 1605.8402 6.86 1 12 0.43 +1Score > 39 indicates identity
Score > 21 indicates homology		 ALSLKSNQALWFVE + Deamidated (NQ)
1745 410.5766 1228.7080 1228.7139 -4.85 1 12 0.87 +1Score > 38 indicates identity
Score > 24 indicates homology		 KTDLSVSVKPR
2299 501.9281 1502.7625 1502.7464 10.7 0 12 0.81 +1Score > 41 indicates identity
Score > 24 indicates homology		 IQQLLSQNQGTIR + 5 Deamidated (NQ)
2796 576.9916 1727.9530 1727.9319 12.2 1 12 0.88 +1Score > 37 indicates identity
Score > 24 indicates homology		 QAIGLTGDLPFIGRDR
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