MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3724 769.7096 2306.1070 2306.1464 -17.1 1 14 0.25 +1Score > 39 indicates identity
Score > 20 indicates homology		 FQSDLKIINDCLDGLIQNAK + 2 Deamidated (NQ)
948 447.7766 893.5386 893.5334 5.84 0 14 0.46 +1Score > 33 indicates identity
Score > 23 indicates homology		 IVLAPEPR
2637 826.4338 1650.8530 1650.8465 4.00 0 14 0.7 +1Score > 40 indicates identity
Score > 25 indicates homology		 ITTVQAAIDFIQSSR + 2 Deamidated (NQ)
2689 836.4338 1670.8530 1670.8475 3.31 1 14 0.31 +1Score > 40 indicates identity
Score > 21 indicates homology		 AVQTLDDLLNAGVEGR + Deamidated (NQ)
3418 1064.5631 2127.1116 2127.0749 17.3 1 14 0.7 +1Score > 38 indicates identity
Score > 25 indicates homology		 IHSTFTIQVEYGTGPHAIR + Deamidated (NQ)
1528 378.8712 1133.5918 1133.5829 7.83 1 14 0.54 +1Score > 42 indicates identity
Score > 24 indicates homology		 GAGNFLAIRSE
1649 594.8226 1187.6306 1187.6397 -7.64 1 14 0.39 +1Score > 41 indicates identity
Score > 22 indicates homology		 LAAQVDLSKNK + 2 Deamidated (NQ)
706 394.2247 786.4348 786.4348 0.081 1 14 0.99 +1Score > 44 indicates identity
Score > 26 indicates homology		 AKQVDAR
2105 701.3835 1400.7524 1400.7486 2.76 0 14 0.57 +1Score > 40 indicates identity
Score > 24 indicates homology		 SAMPTIHYLLQK
840 425.7271 849.4396 849.4344 6.12 0 14 0.51 +1Score > 42 indicates identity
Score > 23 indicates homology		 YNDAVIR
1225 332.8551 995.5435 995.5512 -7.79 0 14 0.28 +1Score > 37 indicates identity
Score > 21 indicates homology		 LSNVHTAVR
1884 649.8458 1297.6770 1297.6738 2.48 1 14 0.38 +1Score > 40 indicates identity
Score > 22 indicates homology		 NVTPNNNIRQK + Deamidated (NQ)
2026 683.8618 1365.7090 1365.7292 -14.8 0 14 0.26 +1Score > 40 indicates identity
Score > 20 indicates homology		 IAAQDYIIGVFR + Deamidated (NQ)
3613 746.4111 2236.2115 2236.2150 -1.59 1 14 0.073 +1Score > 35 indicates identity
Score > 15 indicates homology		 MFLQARAALVTGGALYIVGNR + Oxidation (M)
2570 540.2963 1617.8671 1617.8661 0.63 1 14 0.56 +1Score > 39 indicates identity
Score > 24 indicates homology		 TPGAQLALLQRMYR + Deamidated (NQ)
3093 639.0275 1914.0607 1914.0350 13.4 1 14 0.69 +1Score > 35 indicates identity
Score > 25 indicates homology		 LSIPFTTGLLVGIGETPAE
4414 1055.5167 3163.5283 3163.5282 0.014 1 14 0.092 +1Score > 36 indicates identity
Score > 16 indicates homology		 LAATFQSDIPSKQNQAQNGNQSHLPPQSR + 2 Deamidated (NQ)
1658 597.8581 1193.7016 1193.7019 -0.25 0 14 0.71 +1Score > 33 indicates identity
Score > 25 indicates homology		 QLIVNATLPPK + Deamidated (NQ)
2399 775.4056 1548.7966 1548.8148 -11.7 1 14 0.44 +1Score > 40 indicates identity
Score > 23 indicates homology		 QPSVKYATLVSNDK
2893 597.3125 1788.9157 1788.9444 -16.0 1 14 0.17 +1Score > 40 indicates identity
Score > 18 indicates homology		 AQVQHMVHLLLQLEK + 3 Deamidated (NQ)
1540 570.2638 1138.5130 1138.5117 1.15 1 14 0.16 +1Score > 40 indicates identity
Score > 18 indicates homology		 FSAMGVWNEV
644 382.2098 762.4050 762.4163 -14.8 1 14 0.97 +1Score > 43 indicates identity
Score > 26 indicates homology		 ILFEIE
2288 750.3773 1498.7400 1498.7528 -8.53 1 14 0.46 +1Score > 40 indicates identity
Score > 23 indicates homology		 GDWLVRDPISGQR + Deamidated (NQ)
3294 682.6948 2045.0626 2045.0629 -0.14 1 14 0.42 +1Score > 38 indicates identity
Score > 22 indicates homology		 MAKAHDLIAWNAPHSLVR + Oxidation (M)
1560 384.1909 1149.5509 1149.5410 8.63 1 14 0.36 +1Score > 42 indicates identity
Score > 22 indicates homology		 KAGMIPLQME + Deamidated (NQ); 2 Oxidation (M)
1889 651.8641 1301.7136 1301.7013 9.50 1 14 0.84 +1Score > 40 indicates identity
Score > 25 indicates homology		 MGTNLAIEIALR + Deamidated (NQ)
2416 519.3010 1554.8812 1554.8617 12.5 0 14 0.97 +1Score > 36 indicates identity
Score > 26 indicates homology		 VTQLLSLLANVQQE
2878 593.6105 1777.8097 1777.7842 14.3 0 14 0.2 +1Score > 39 indicates identity
Score > 19 indicates homology		 VQIWSCSGGANQQWR + 2 Deamidated (NQ)
996 305.8286 914.4640 914.4821 -19.8 0 14 0.79 +1Score > 41 indicates identity
Score > 25 indicates homology		 NSKPVNVR + 2 Deamidated (NQ)
1631 591.3682 1180.7218 1180.7179 3.31 1 14 0.48 +1Score > 31 indicates identity
Score > 23 indicates homology		 NKLTLGLPTPK
2382 772.4093 1542.8040 1542.8140 -6.48 0 14 0.64 +1Score > 40 indicates identity
Score > 24 indicates homology		 LAIQNSQIASAIALE + 2 Deamidated (NQ)
2869 887.4535 1772.8924 1772.8655 15.2 1 14 0.23 +1Score > 40 indicates identity
Score > 20 indicates homology		 VNFSVNFKCLSSQIK + 3 Deamidated (NQ)
646 382.2277 762.4408 762.4527 -15.6 0 14 0.89 +1Score > 40 indicates identity
Score > 26 indicates homology		 IIYLLE
4247 967.8488 2900.5246 2900.5793 -18.9 1 14 0.12 +1Score > 35 indicates identity
Score > 17 indicates homology		 MSIASQIDHTALKPHTQKADILQLIK + Deamidated (NQ)
346 311.6824 621.3502 621.3486 2.62 0 14 0.45 +1Score > 38 indicates identity
Score > 23 indicates homology		 ATGFVK
801 418.2250 834.4354 834.4269 10.2 0 14 0.88 +1Score > 42 indicates identity
Score > 25 indicates homology		 AAQTMGLK + Oxidation (M)
4135 922.8423 2765.5051 2765.4575 17.2 1 14 0.058 +1Score > 33 indicates identity
Score > 14 indicates homology		 VIIMADADVDGSHIRTLLLTFFFR + Oxidation (M)
1917 439.8845 1316.6317 1316.6217 7.59 0 14 0.71 +1Score > 41 indicates identity
Score > 25 indicates homology		 DLLGTNMMLHR + Deamidated (NQ); Oxidation (M)
955 449.2812 896.5478 896.5331 16.5 0 13 0.84 +1Score > 34 indicates identity
Score > 25 indicates homology		 IKPQAVIE
1743 615.3326 1228.6506 1228.6485 1.74 0 13 0.95 +1Score > 41 indicates identity
Score > 26 indicates homology		 ASEPLMLAAQAK
3191 996.0227 1990.0308 1990.0081 11.4 1 13 0.93 +1Score > 39 indicates identity
Score > 26 indicates homology		 QTTEPNYPEMLLIAINK + Oxidation (M)
3720 1154.0563 2306.0980 2306.0962 0.79 1 13 0.075 +1Score > 39 indicates identity
Score > 15 indicates homology		 MNNLIKAYAAGVMSAAFLFGSE + 2 Deamidated (NQ)
3712 1152.0729 2302.1312 2302.1176 5.92 1 13 0.24 +1Score > 39 indicates identity
Score > 20 indicates homology		 SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ)
1195 491.7535 981.4924 981.4767 16.0 1 13 0.78 +1Score > 40 indicates identity
Score > 25 indicates homology		 LYVDSETR
1969 671.3803 1340.7460 1340.7551 -6.76 0 13 0.22 +1Score > 38 indicates identity
Score > 19 indicates homology		 LNPSLVISLSQGL + Deamidated (NQ)
3883 818.4375 2452.2907 2452.2672 9.59 1 13 0.067 +1Score > 36 indicates identity
Score > 14 indicates homology		 TFLLKPQTFMNLSGQAIGEALR + 2 Deamidated (NQ); Oxidation (M)
970 451.2557 900.4968 900.4851 13.1 1 13 0.86 +1Score > 42 indicates identity
Score > 25 indicates homology		 NAPCALKK
1821 635.8845 1269.7544 1269.7405 11.0 0 13 0.8 +1Score > 35 indicates identity
Score > 25 indicates homology		 NIASAVQLSVIR
2276 499.2202 1494.6388 1494.6297 6.10 1 13 0.21 +1Score > 37 indicates identity
Score > 19 indicates homology		 AEPTEQSFMQPGR + 2 Deamidated (NQ); Oxidation (M)
3050 627.9974 1880.9704 1880.9493 11.2 1 13 0.69 +1Score > 39 indicates identity
Score > 24 indicates homology		 THGHLGQSAILSEVYNR
815 421.7233 841.4320 841.4406 -10.2 0 13 0.68 +1Score > 39 indicates identity
Score > 24 indicates homology		 NTPNQIR
3310 1030.5334 2059.0522 2059.0884 -17.6 1 13 0.86 +1Score > 39 indicates identity
Score > 25 indicates homology		 ANLVFGGTNLQIVMVPGRR + 2 Deamidated (NQ); Oxidation (M)
1612 391.8609 1172.5609 1172.5648 -3.37 1 13 0.98 +1Score > 40 indicates identity
Score > 26 indicates homology		 HFVEMEPIR + Oxidation (M)
2296 752.3786 1502.7426 1502.7287 9.30 1 13 0.81 +1Score > 41 indicates identity
Score > 25 indicates homology		 GLEVCIVTTANTPE
1880 432.9161 1295.7265 1295.7193 5.57 1 13 1 +1Score > 37 indicates identity
Score > 26 indicates homology		 MSMLSTLLLKK + 2 Oxidation (M)
784 414.7234 827.4322 827.4388 -7.97 1 13 0.82 +1Score > 38 indicates identity
Score > 25 indicates homology		 IPNNEIK + Deamidated (NQ)
2492 794.9114 1587.8082 1587.7933 9.41 1 13 0.15 +1Score > 41 indicates identity
Score > 18 indicates homology		 LKGFWGQDVAPIQE + Deamidated (NQ)
2804 866.8899 1731.7652 1731.7807 -8.95 0 13 0.74 +1Score > 39 indicates identity
Score > 25 indicates homology		 NDNPLVDMQAMSPTAK + Deamidated (NQ)
3435 1068.5624 2135.1102 2135.1409 -14.3 1 13 0.76 +1Score > 38 indicates identity
Score > 25 indicates homology		 LADGAIDLYAMVVVLSRASR + Oxidation (M)
464 341.6986 681.3826 681.3810 2.47 0 13 0.23 +1Score > 37 indicates identity
Score > 19 indicates homology		 IDVHAK
2366 510.9543 1529.8411 1529.8678 -17.4 1 13 0.3 +1Score > 38 indicates identity
Score > 21 indicates homology		 TYASLLPRNNLIR
1620 589.2977 1176.5808 1176.6026 -18.5 1 13 0.98 +1Score > 42 indicates identity
Score > 26 indicates homology		 AIAYIEANPSK + Deamidated (NQ)
4398 784.3981 3133.5633 3133.5028 19.3 1 13 0.14 +1Score > 36 indicates identity
Score > 17 indicates homology		 FEVVLYLNQMGVFNFVDDGSLPGCAVLK + 3 Deamidated (NQ)
989 456.7456 911.4766 911.4825 -6.39 1 13 0.61 +1Score > 39 indicates identity
Score > 24 indicates homology		 KTAGSSHPK
3457 1073.5994 2145.1842 2145.1933 -4.20 0 13 0.14 +1Score > 34 indicates identity
Score > 17 indicates homology		 TVLHLAVQAANPTLVQLLLE + 3 Deamidated (NQ)
904 437.7178 873.4210 873.4344 -15.3 1 13 0.68 +1Score > 42 indicates identity
Score > 24 indicates homology		 LKHNFSE
1373 353.8620 1058.5642 1058.5621 1.93 1 13 0.8 +1Score > 42 indicates identity
Score > 25 indicates homology		 EHVVSVPHR
2009 679.8273 1357.6400 1357.6374 1.92 1 13 0.75 +1Score > 40 indicates identity
Score > 24 indicates homology		 NPDNATWNRIR + 2 Deamidated (NQ)
549 717.3862 716.3789 716.3891 -14.2 0 13 0.85 +1Score > 43 indicates identity
Score > 25 indicates homology		 QMPLTK
2029 683.9158 1365.8170 1365.7980 14.0 1 13 0.25 +1Score > 31 indicates identity
Score > 20 indicates homology		 DKVQIVQPLLGR + Deamidated (NQ)
2830 877.4651 1752.9156 1752.8902 14.5 1 13 0.92 +1Score > 39 indicates identity
Score > 25 indicates homology		 ILLENGMPVTAHLCGK + Deamidated (NQ)
1648 594.7985 1187.5824 1187.5670 13.0 0 13 0.95 +1Score > 41 indicates identity
Score > 25 indicates homology		 GVVLVDDAGQSE
2103 701.3827 1400.7508 1400.7623 -8.20 1 13 0.58 +1Score > 40 indicates identity
Score > 23 indicates homology		 SVTQVNRDLLAGK + Deamidated (NQ)
2286 749.9395 1497.8644 1497.8402 16.2 1 13 0.67 +1Score > 34 indicates identity
Score > 24 indicates homology		 ISIAGRLVADLLQE + Deamidated (NQ)
1966 447.2601 1338.7585 1338.7368 16.2 1 13 0.31 +1Score > 36 indicates identity
Score > 21 indicates homology		 RSSHITVVVADR
2805 866.8903 1731.7660 1731.7807 -8.49 0 13 0.7 +1Score > 39 indicates identity
Score > 24 indicates homology		 NDNPLVDMQAMSPTAK + Deamidated (NQ)
1764 412.8922 1235.6548 1235.6775 -18.4 0 13 0.93 +1Score > 40 indicates identity
Score > 25 indicates homology		 YRPYAVAQLR
842 426.2126 850.4106 850.4185 -9.18 1 13 0.75 +1Score > 42 indicates identity
Score > 24 indicates homology		 IEQFASR + Deamidated (NQ)
2803 866.8898 1731.7650 1731.7807 -9.06 0 13 0.69 +1Score > 39 indicates identity
Score > 24 indicates homology		 NDNPLVDMQAMSPTAK + Deamidated (NQ)
535 357.2144 712.4142 712.4119 3.26 0 13 0.96 +1Score > 38 indicates identity
Score > 25 indicates homology		 DLAGIPK
2088 464.2613 1389.7621 1389.7364 18.4 1 13 0.33 +1Score > 39 indicates identity
Score > 21 indicates homology		 INQQVLQFSRR + 2 Deamidated (NQ)
2239 736.8673 1471.7200 1471.7266 -4.47 1 13 0.99 +1Score > 41 indicates identity
Score > 26 indicates homology		 QQINAIESANINR + 2 Deamidated (NQ)
994 457.7766 913.5386 913.5345 4.52 0 13 0.79 +1Score > 40 indicates identity
Score > 25 indicates homology		 IATIGVGAGR
3129 649.7078 1946.1016 1946.0724 15.0 1 13 0.22 +1Score > 32 indicates identity
Score > 19 indicates homology		 EIGAIAKPDLIQNAPGLPK + 2 Deamidated (NQ)
466 342.2275 682.4404 682.4378 3.94 0 13 0.68 +1Score > 33 indicates identity
Score > 24 indicates homology		 VPAVIGK
1059 931.4756 930.4683 930.4770 -9.36 1 13 0.17 +1Score > 42 indicates identity
Score > 18 indicates homology		 KGDNQGALK + Deamidated (NQ)
1337 347.2242 1038.6508 1038.6477 2.94 1 13 0.6 +1Score > 32 indicates identity
Score > 23 indicates homology		 LYIYKVLK
903 437.7170 873.4194 873.4344 -17.2 1 13 0.32 +1Score > 41 indicates identity
Score > 21 indicates homology		 LKHNFSE
2362 765.4351 1528.8556 1528.8712 -10.2 1 13 0.68 +1Score > 37 indicates identity
Score > 24 indicates homology		 LQTAITLIDELTAK
2067 693.8627 1385.7108 1385.7303 -14.0 0 13 0.79 +1Score > 41 indicates identity
Score > 25 indicates homology		 DQLWLTGDLVAR
2338 758.3661 1514.7176 1514.7035 9.36 1 13 0.086 +1Score > 41 indicates identity
Score > 15 indicates homology		 SESMYQSQLLASR + Oxidation (M)
4075 901.8047 2702.3923 2702.3731 7.10 1 13 0.12 +1Score > 37 indicates identity
Score > 16 indicates homology		 AVQFIDLLQKVYNDFGFNDILVK + 4 Deamidated (NQ)
246 529.3475 528.3402 528.3384 3.52 0 13 0.8 +1Score > 41 indicates identity
Score > 25 indicates homology		 AVIAR
1909 438.9368 1313.7886 1313.8031 -11.0 1 13 0.87 +1Score > 33 indicates identity
Score > 25 indicates homology		 RVAISSLLATGVK
491 348.7067 695.3988 695.3966 3.25 0 13 0.37 +1Score > 36 indicates identity
Score > 21 indicates homology		 APAAAPAK
2624 823.4124 1644.8102 1644.7995 6.53 0 13 0.58 +1Score > 41 indicates identity
Score > 23 indicates homology		 VLNSTYFNGLSQSSK + Deamidated (NQ)
2150 712.3599 1422.7052 1422.6991 4.33 0 13 0.76 +1Score > 41 indicates identity
Score > 24 indicates homology		 ATSVPGPTQATAPPE
1588 387.8892 1160.6458 1160.6587 -11.1 0 13 0.79 +1Score > 39 indicates identity
Score > 24 indicates homology		 MLLITANTLR + Oxidation (M)
3301 514.7565 2054.9969 2055.0194 -10.9 1 13 0.23 +1Score > 40 indicates identity
Score > 19 indicates homology		 IDPNNNVIQAMILVPTRE + 3 Deamidated (NQ); Oxidation (M)
329 308.2139 614.4132 614.4115 2.80 1 13 0.62 +1Score > 38 indicates identity
Score > 23 indicates homology		 KGAIVK
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