MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 141–150 (out of 179)


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+141

Accession Score Description
1 AMPA_PSEPK 33 Probable cytosol aminopeptidase OS=Pseudomonas putida (strain KT2440) GN=pepA PE=3 SV=1

+142

Accession Score Description
1 MFD_STAEQ 32 Transcription-repair-coupling factor OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=mfd PE=3 SV=1

+143

Accession Score Description
1 CAPB_PSEFR 32 Cold shock protein capB OS=Pseudomonas fragi GN=capB PE=1 SV=1

+144

Accession Score Description
1 LPXH_AZOSE 31 UDP-2,3-diacylglucosamine hydrolase OS=Azoarcus sp. (strain EbN1) GN=lpxH PE=3 SV=1

+145

Accession Score Description
1 BIKB_CYAP8 31 Putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase OS=Cyanothece sp. (strain PCC 8801) GN=PCC8801_0067 PE=3 SV=1

+146

Accession Score Description
1 PRP28_YARLI 31 Pre-mRNA-splicing ATP-dependent RNA helicase PRP28 OS=Yarrowia lipolytica GN=PRP28 PE=3 SV=1

+147

Accession Score Description
1 SSB_PSEPK 31 Single-stranded DNA-binding protein OS=Pseudomonas putida (strain KT2440) GN=ssb PE=3 SV=1

+148

Accession Score Description
1 ACSA_ECOL6 30 Acetyl-coenzyme A synthetase OS=Escherichia coli O6 GN=acs PE=3 SV=1

+149

Accession Score Description
1 IDH_AZOVI 30 Isocitrate dehydrogenase [NADP] OS=Azotobacter vinelandii GN=icd PE=1 SV=5

+150

Accession Score Description
1 RS1_HAEIN 30 30S ribosomal protein S1 OS=Haemophilus influenzae GN=rpsA PE=3 SV=1
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