MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1–100 (out of 3643)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
257 544.3478 543.3405 543.3380 4.57 0 42 0.091 +1Score > 44 indicates identity AGAVVK
305 601.3686 600.3613 600.3707 -15.7 1 41 0.094 +1Score > 44 indicates identity AGRVAK
869 429.7598 857.5050 857.4971 9.32 1 41 0.06 +1Score > 41 indicates identity GAELVLTR
510 351.7064 701.3982 701.3959 3.28 1 40 0.11 +1Score > 43 indicates identity VEVQVK + Deamidated (NQ)
477 689.3865 688.3792 688.3755 5.36 1 39 0.13 +1Score > 43 indicates identity DDKAIK
1367 528.7955 1055.5764 1055.5836 -6.75 1 39 0.069 +1Score > 40 indicates identity GLAAGAQRVGR + Deamidated (NQ)
787 415.2447 828.4748 828.4705 5.28 1 38 0.13 -1Score > 42 indicates identity ENLALIR + Deamidated (NQ)
1145 962.5447 961.5374 961.5192 18.9 1 38 0.07 +1Score > 39 indicates identity SSKLVSANR + Deamidated (NQ)
321 307.7038 613.3930 613.3911 3.10 0 38 0.091 +1Score > 40 indicates identity IGGVLR
2137 472.5768 1414.7086 1414.7052 2.38 0 37 0.087 +1Score > 41 indicates identity
Score > 39 indicates homology		 TANAIVSQQTPQR + 2 Deamidated (NQ)
471 343.2227 684.4308 684.4282 3.84 0 37 0.067 +1Score > 38 indicates identity LAALAAR
1329 1035.5897 1034.5824 1034.6012 -18.1 1 37 0.082 +1Score > 38 indicates identity IEIYIVGTK
876 430.7227 859.4308 859.4174 15.6 1 37 0.08 +1Score > 43 indicates identity
Score > 38 indicates homology		 IIQENIE + 2 Deamidated (NQ)
556 361.6787 721.3428 721.3548 -16.5 0 36 0.084 +1Score > 41 indicates identity
Score > 38 indicates homology		 YPTWR
641 761.4064 760.3991 760.4079 -11.5 0 36 0.12 +1Score > 43 indicates identity
Score > 40 indicates homology		 SGNATALK
543 358.2287 714.4428 714.4388 5.65 0 36 0.16 +1Score > 41 indicates identity LASVGIR
328 615.3590 614.3517 614.3500 2.82 0 36 0.14 +1Score > 40 indicates identity QAGIAR
1127 478.7834 955.5522 955.5451 7.52 1 36 0.13 +1Score > 40 indicates identity VAEQLAAVR
452 338.1885 674.3624 674.3711 -12.9 0 36 0.27 +1Score > 43 indicates identity TIGSAAR
411 331.6968 661.3790 661.3833 -6.36 0 36 0.11 +1Score > 39 indicates identity ISLMAK
1065 468.2758 934.5370 934.5276 10.1 0 36 0.077 +1Score > 37 indicates identity NFLIPFGK
1325 1035.5873 1034.5800 1034.5760 3.86 1 36 0.089 +1Score > 38 indicates identity
Score > 38 indicates homology		 IFGNLDTKK
1100 474.2392 946.4638 946.4542 10.2 1 36 0.096 +1Score > 42 indicates identity
Score > 38 indicates homology		 EAVQCAIR + Deamidated (NQ)
959 450.2866 898.5586 898.5488 11.0 0 35 0.072 +1Score > 37 indicates identity AVLDQLLK
209 487.3019 486.2946 486.2914 6.61 0 35 0.29 +1Score > 42 indicates identity AVAAR
763 408.2360 814.4574 814.4548 3.19 1 35 0.33 +1Score > 43 indicates identity EIIIDGR
1045 928.4190 927.4117 927.3942 18.9 1 35 0.11 +1Score > 38 indicates identity
Score > 38 indicates homology		 EFMMQSR
447 673.4272 672.4199 672.4170 4.31 0 35 0.18 +1Score > 40 indicates identity AVSVAVK
1227 498.7794 995.5442 995.5512 -7.01 0 35 0.1 +1Score > 37 indicates identity LSNVHTAVR
479 689.3940 688.3867 688.3755 16.3 1 34 0.39 +1Score > 43 indicates identity DDKAIK
1203 494.2796 986.5446 986.5509 -6.33 0 34 0.083 +1Score > 42 indicates identity
Score > 36 indicates homology		 VVATAAAATGR
1241 501.2883 1000.5620 1000.5553 6.76 1 34 0.27 +1Score > 41 indicates identity QATLEQALK
1264 505.7324 1009.4502 1009.4464 3.77 1 34 0.12 +1Score > 40 indicates identity
Score > 37 indicates homology		 QYANDRNK + 2 Deamidated (NQ)
444 337.2046 672.3946 672.3919 4.14 0 34 0.08 +1Score > 41 indicates identity
Score > 36 indicates homology		 SIGIQR
761 407.7539 813.4932 813.5072 -17.2 0 34 0.18 +1Score > 41 indicates identity
Score > 39 indicates homology		 NIILVSR
1855 643.3500 1284.6854 1284.6925 -5.49 0 34 0.24 +1Score > 40 indicates identity DVLLLDAQAQAK + Deamidated (NQ)
1258 505.2549 1008.4952 1008.4876 7.59 1 34 0.34 +1Score > 42 indicates identity EGAQAYLTR + Deamidated (NQ)
760 407.7445 813.4744 813.4708 4.45 0 34 0.31 +1Score > 41 indicates identity LVANQIR + Deamidated (NQ)
976 453.2409 904.4672 904.4502 18.9 0 34 0.093 +1Score > 42 indicates identity
Score > 36 indicates homology		 LDSSATPSK
1805 631.3571 1260.6996 1260.6747 19.8 1 34 0.19 +1Score > 39 indicates identity MKLLNQLIDR + 2 Deamidated (NQ); Oxidation (M)
454 675.3792 674.3719 674.3599 17.8 0 33 0.47 +1Score > 42 indicates identity SSPSVAK
304 601.3683 600.3610 600.3707 -16.2 1 33 0.51 +1Score > 44 indicates identity
Score > 43 indicates homology		 KGAVAR
1513 566.2874 1130.5602 1130.5567 3.10 1 33 0.2 +1Score > 42 indicates identity
Score > 39 indicates homology		 VVELNASDQR + Deamidated (NQ)
268 559.3222 558.3149 558.3238 -15.9 1 33 0.86 +1Score > 45 indicates identity
Score > 45 indicates homology		 AGAKGR
884 432.2285 862.4424 862.4396 3.28 0 33 0.059 +1Score > 42 indicates identity
Score > 33 indicates homology		 GSLDTSGVK
1327 1035.5879 1034.5806 1034.6012 -19.9 1 32 0.22 +1Score > 38 indicates identity IEIYIVGTK
1903 657.3499 1312.6852 1312.6623 17.5 1 32 0.16 +1Score > 40 indicates identity
Score > 37 indicates homology		 QGLARDGKPIQE + 2 Deamidated (NQ)
453 675.3706 674.3633 674.3599 5.09 1 32 0.59 +1Score > 43 indicates identity AGTVEAK
759 407.7445 813.4744 813.4821 -9.35 1 32 0.42 +1Score > 41 indicates identity QKQALAR
839 425.7249 849.4352 849.4344 0.94 0 32 0.16 +1Score > 42 indicates identity
Score > 37 indicates homology		 YNDAVIR
597 371.2543 740.4940 740.4908 4.33 0 32 0.067 +1Score > 33 indicates identity VLAVALR
603 372.2225 742.4304 742.4337 -4.42 1 32 0.59 +1Score > 42 indicates identity GLGEVIR
1272 337.8707 1010.5903 1010.5873 2.96 0 32 0.13 +1Score > 36 indicates identity VGATPIGLGAR
1803 631.3539 1260.6932 1260.6747 14.7 1 32 0.3 +1Score > 39 indicates identity MKLLNQLIDR + 2 Deamidated (NQ); Oxidation (M)
788 415.7420 829.4694 829.4810 -13.9 1 32 0.82 +1Score > 43 indicates identity KIIDWR
1240 500.7765 999.5384 999.5349 3.55 0 31 0.12 +1Score > 40 indicates identity
Score > 35 indicates homology		 VANLGNLGDK
1421 362.1938 1083.5596 1083.5713 -10.8 1 31 0.39 +1Score > 40 indicates identity FTPLHEAAAK
1235 499.3064 996.5982 996.5855 12.8 1 31 0.13 +1Score > 35 indicates identity EVIAIDLPK
405 329.7115 657.4084 657.4061 3.55 0 31 0.21 +1Score > 41 indicates identity
Score > 37 indicates homology		 IDVALK
1318 516.2624 1030.5102 1030.5043 5.75 0 31 0.73 +1Score > 42 indicates identity LTSNEPSQR
1820 635.8331 1269.6516 1269.6353 12.9 1 31 0.091 +1Score > 40 indicates identity
Score > 33 indicates homology		 QIPDWNIEVR + Deamidated (NQ)
1575 578.8224 1155.6302 1155.6248 4.74 0 31 0.077 +1Score > 40 indicates identity
Score > 32 indicates homology		 SPATVLADLNR
1090 471.7927 941.5708 941.5545 17.3 1 31 0.33 +1Score > 38 indicates identity LIQAVELR + Deamidated (NQ)
864 429.2467 856.4788 856.4767 2.56 0 31 0.52 +1Score > 40 indicates identity LVAQGVNR + Deamidated (NQ)
982 454.7471 907.4796 907.4651 16.1 1 31 0.19 +1Score > 41 indicates identity
Score > 36 indicates homology		 GANLEYLK + Deamidated (NQ)
931 444.2527 886.4908 886.4872 4.10 1 31 0.65 +1Score > 42 indicates identity
Score > 41 indicates homology		 DGLALRDK
618 376.1916 750.3686 750.3582 13.9 0 31 0.36 +1Score > 41 indicates identity
Score > 39 indicates homology		 MTQIDK + Oxidation (M)
934 444.7447 887.4748 887.4712 4.09 0 31 0.7 +1Score > 43 indicates identity
Score > 42 indicates homology		 NNDTLIAK
665 771.4381 770.4308 770.4286 2.84 0 31 0.12 +1Score > 39 indicates identity
Score > 34 indicates homology		 LGQSLPR + Deamidated (NQ)
942 894.4704 893.4631 893.4607 2.75 1 30 0.53 +1Score > 41 indicates identity
Score > 40 indicates homology		 GISEGLYR
673 775.4346 774.4273 774.4236 4.85 0 30 0.57 +1Score > 43 indicates identity
Score > 40 indicates homology		 GNSLTGVK
828 423.2499 844.4852 844.4879 -3.12 1 30 0.19 +1Score > 40 indicates identity
Score > 36 indicates homology		 ISSGGLRR
586 366.7047 731.3948 731.3926 3.06 0 30 0.66 +1Score > 44 indicates identity
Score > 41 indicates homology		 TGTGQIR
588 367.6902 733.3658 733.3718 -8.17 1 30 0.94 +1Score > 42 indicates identity STKNQR + Deamidated (NQ)
490 348.1729 694.3312 694.3286 3.84 1 30 0.62 +1Score > 41 indicates identity IDEYR
1558 575.2997 1148.5848 1148.5747 8.84 1 30 0.22 +1Score > 42 indicates identity
Score > 36 indicates homology		 KQIQQMVQK + 3 Deamidated (NQ); Oxidation (M)
1316 516.2615 1030.5084 1030.4931 14.9 0 30 0.46 +1Score > 41 indicates identity
Score > 39 indicates homology		 LTQNLAGGQE + Deamidated (NQ)
786 415.2446 828.4746 828.4705 5.04 1 30 0.35 +1Score > 42 indicates identity
Score > 38 indicates homology		 ENLALIR + Deamidated (NQ)
1801 629.8572 1257.6998 1257.6816 14.5 0 30 0.51 +1Score > 39 indicates identity LVIAVANDSNIK + 2 Deamidated (NQ)
1317 516.2615 1030.5084 1030.4931 14.9 0 30 0.46 +1Score > 41 indicates identity
Score > 39 indicates homology		 LTQNLAGGQE + Deamidated (NQ)
379 643.4157 642.4084 642.4064 3.10 0 30 0.46 +1Score > 41 indicates identity
Score > 39 indicates homology		 AAAAVIK
1447 549.3157 1096.6168 1096.6063 9.64 1 30 0.08 +1Score > 37 indicates identity
Score > 31 indicates homology		 LPDIGPKMAR
833 424.7001 847.3856 847.3937 -9.46 1 30 0.16 +1Score > 41 indicates identity
Score > 34 indicates homology		 DDTWRR
538 357.2519 712.4892 712.4847 6.41 0 30 0.16 +1Score > 34 indicates identity NIIILK
539 357.2520 712.4894 712.4847 6.69 0 30 0.16 +1Score > 34 indicates identity NIIILK
2086 464.2609 1389.7609 1389.7504 7.57 1 30 0.16 +1Score > 39 indicates identity
Score > 34 indicates homology		 GLFVAISNQEIAK + Deamidated (NQ)
385 645.3600 644.3527 644.3493 5.28 1 30 0.74 +1Score > 45 indicates identity
Score > 41 indicates homology		 QGEIAK
1652 595.3419 1188.6692 1188.6615 6.52 0 29 0.35 +1Score > 39 indicates identity
Score > 37 indicates homology		 TVHPLADLAPR
401 328.6911 655.3676 655.3765 -13.6 0 29 0.18 +1Score > 37 indicates identity
Score > 34 indicates homology		 IDRPR
628 380.2228 758.4310 758.4286 3.18 1 29 0.27 +1Score > 44 indicates identity
Score > 36 indicates homology		 LVETAAR
837 425.2477 848.4808 848.4868 -7.02 1 29 0.59 +1Score > 40 indicates identity LYRQIR + Deamidated (NQ)
634 380.2548 758.4950 758.4902 6.43 0 29 0.062 +1Score > 30 indicates identity VISILSK
2104 467.9246 1400.7520 1400.7762 -17.3 1 29 0.15 +1Score > 40 indicates identity
Score > 34 indicates homology		 TIDETLDLLLAGK
2467 790.4510 1578.8874 1578.8729 9.20 1 29 0.22 +1Score > 35 indicates identity ELQANVVHLSLLSR + Deamidated (NQ)
537 357.2516 712.4886 712.4847 5.57 0 29 0.17 +1Score > 34 indicates identity NIIILK
723 398.2232 794.4318 794.4286 4.04 0 29 0.57 +1Score > 39 indicates identity AVSSYLR
493 350.7040 699.3934 699.4027 -13.3 0 29 0.47 +1Score > 39 indicates identity
Score > 38 indicates homology		 AAQLAAR
1064 467.7740 933.5334 933.5171 17.5 0 29 0.49 +1Score > 38 indicates identity VLLANYLE
2346 506.9544 1517.8414 1517.8202 14.0 1 29 0.12 +1Score > 37 indicates identity
Score > 32 indicates homology		 VKFNVINTAVQGAR + 2 Deamidated (NQ)
547 358.7200 715.4254 715.4228 3.65 0 29 0.21 +1Score > 43 indicates identity
Score > 35 indicates homology		 ITVDLR
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