MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 91–100 (out of 179)


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+91

Accession Score Description
1 RL15_PSEP1 53 50S ribosomal protein L15 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplO PE=3 SV=1

-92

Accession Score Description
1 RS3_PSEE4 53 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
Score Mass Matches Sequences emPAI
92.1 RS3_PSEE4 53 25709 20 (3) 9 (3) 0.30
30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1
3 samesets of RS3_PSEE4
RS3_PSEP1 53 25709 20 (3) 9 (3) 0.30
30S ribosomal protein S3 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsC PE=3 SV=1
RS3_PSEPK 53 25709 20 (3) 9 (3) 0.30
30S ribosomal protein S3 OS=Pseudomonas putida (strain KT2440) GN=rpsC PE=3 SV=2
RS3_PSEU5 53 25730 20 (3) 9 (3) 0.30
30S ribosomal protein S3 OS=Pseudomonas stutzeri (strain A1501) GN=rpsC PE=3 SV=1

-20 peptide matches (13 non-duplicate, 7 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
406   659.3854 658.3781 658.3762 2.89 0 23 0.34 +1Score > 44 indicates identity
Score > 31 indicates homology
K.IQVSGR.L
407 +1 330.1965 658.3784 658.3762 3.38 0 32 0.17 +1Score > 42 indicates identity
Score > 37 indicates homology
K.IQVSGR.L
484 +1 346.7130 691.4114 691.4057 8.27 0 30 0.051 +1Score > 37 indicates identity
Score > 30 indicates homology
U K.VWIFK.G
623   377.6807 753.3468 753.3446 3.02 1 19 0.76 +1Score > 39 indicates identity
Score > 31 indicates homology
R.TEWYR.E
705 +1 393.7283 785.4420 785.4395 3.21 1 37 0.17 +1Score > 43 indicates identity
Score > 42 indicates homology
R.LGGAEIAR.T
721   398.1989 794.3832 794.3810 2.81 1 12 1.2 +2Score > 41 indicates identity
Score > 25 indicates homology
R.EYLQDK.L
802   418.2628 834.5110 834.5076 4.17 0 24 0.33 +1Score > 33 indicates identity
Score > 32 indicates homology
R.VPLHTLR.A
1837   320.1833 1276.7041 1276.7000 3.21 0 16 0.46 +1Score > 38 indicates identity
Score > 25 indicates homology
U R.IDIHRPAQTAR.I
1838 +1 426.5754 1276.7044 1276.7000 3.43 0 15 0.2 +1Score > 38 indicates identity
Score > 21 indicates homology
U R.IDIHRPAQTAR.I
1839   639.3601 1276.7056 1276.7000 4.43 0 18 0.37 +1Score > 38 indicates identity
Score > 26 indicates homology
U R.IDIHRPAQTAR.I
2251 +2 369.7336 1474.9053 1474.8984 4.69 0 10 1 +1Score > 26 indicates identity
Score > 22 indicates homology
U R.ITIHTARPGIVIGK.K
2252 +1 492.6426 1474.9060 1474.8984 5.15 0 23 0.018 +1Score > 25 indicates identity
Score > 18 indicates homology
U R.ITIHTARPGIVIGK.K
3601   744.3666 2230.0780 2230.0430 15.7 1 46 0.00023 +1Score > 39 indicates identity
Score > 23 indicates homology
U R.ADIDYNTYEAHTTYGVIGVK.V + Deamidated (NQ)

3 subsets and intersections (251 subset proteins in total)

Score Mass Subset of
RR3_CHLMO 30 0 92.1
description
+231 samesets of RR3_CHLMO
RS3_HAHCH 30 25467 92.1
30S ribosomal protein S3 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsC PE=3 SV=1
11 samesets of RS3_HAHCH
RS3_PSE14 30 25737
30S ribosomal protein S3 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsC PE=3 SV=1
RS3_PSEA7 30 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsC PE=3 SV=1
RS3_PSEAB 30 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsC PE=3 SV=1
RS3_PSEAE 30 25822
30S ribosomal protein S3 OS=Pseudomonas aeruginosa GN=rpsC PE=3 SV=1
RS3_PSEF5 30 25676
30S ribosomal protein S3 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsC PE=3 SV=1
RS3_PSEPF 30 25737
30S ribosomal protein S3 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsC PE=3 SV=1
RS3_PSESM 30 25737
30S ribosomal protein S3 OS=Pseudomonas syringae pv. tomato GN=rpsC PE=3 SV=1
RS3_PSEU2 30 25737
30S ribosomal protein S3 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsC PE=3 SV=1
RS3_PSEMY 30 25525
30S ribosomal protein S3 OS=Pseudomonas mendocina (strain ymp) GN=rpsC PE=3 SV=1
RS3_MARAV 30 25498
30S ribosomal protein S3 OS=Marinobacter aquaeolei (strain ATCC 700491 / DSM 11845 / VT8) GN=rpsC PE=3 SV=1
RS3_NITOC 30 24932
30S ribosomal protein S3 OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=rpsC PE=3 SV=1
RS3_CHRSD 23 26097 92.1
30S ribosomal protein S3 OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=rpsC PE=3 SV=1
6 samesets of RS3_CHRSD
RS3_FRATF 23 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) GN=rpsC PE=3 SV=1
RS3_FRATH 23 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain LVS) GN=rpsC PE=3 SV=1
RS3_FRATM 23 24848
30S ribosomal protein S3 OS=Francisella tularensis subsp. mediasiatica (strain FSC147) GN=rpsC PE=3 SV=1
RS3_FRATN 23 24792
30S ribosomal protein S3 OS=Francisella tularensis subsp. novicida (strain U112) GN=rpsC PE=3 SV=1
RS3_FRATO 23 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. holarctica (strain OSU18) GN=rpsC PE=3 SV=1
RS3_FRATW 23 24862
30S ribosomal protein S3 OS=Francisella tularensis subsp. tularensis (strain WY96-3418) GN=rpsC PE=3 SV=1

+93

Accession Score Description
1 XIRP1_CHICK 50 Xin actin-binding repeat-containing protein 1 OS=Gallus gallus GN=Xirp1 PE=2 SV=3

+94

Accession Score Description
1 ODP1_PSEAE 50 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

+95

Accession Score Description
1 PURA_PSEFS 49 Adenylosuccinate synthetase OS=Pseudomonas fluorescens (strain SBW25) GN=purA PE=3 SV=1

+96

Accession Score Description
1 DNAB_MYCPN 49 Replicative DNA helicase OS=Mycoplasma pneumoniae GN=dnaB PE=1 SV=1

+97

Accession Score Description
1 BETI_PROMH 48 HTH-type transcriptional regulator betI OS=Proteus mirabilis (strain HI4320) GN=betI PE=3 SV=1

+98

Accession Score Description
1 Y396_PSEPK 48 UPF0229 protein PP_0396 OS=Pseudomonas putida (strain KT2440) GN=PP_0396 PE=3 SV=1

+99

Accession Score Description
1 ISPD_ALKMQ 48 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1

+100

Accession Score Description
1 ARGJ_OCEIH 48 Arginine biosynthesis bifunctional protein argJ OS=Oceanobacillus iheyensis GN=argJ PE=3 SV=1
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