MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 91–100 (out of 179)

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-91

Accession Score Description
1 RL15_PSEP1 53 50S ribosomal protein L15 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplO PE=3 SV=1
Score Mass Matches Sequences emPAI
91.1 RL15_PSEP1 53 15180 5 (3) 4 (3) 0.54
50S ribosomal protein L15 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplO PE=3 SV=1
3 samesets of RL15_PSEP1
RL15_PSEPG 53 15180 5 (3) 4 (3) 0.54
50S ribosomal protein L15 OS=Pseudomonas putida (strain GB-1) GN=rplO PE=3 SV=1
RL15_PSEPK 53 15180 5 (3) 4 (3) 0.54
50S ribosomal protein L15 OS=Pseudomonas putida (strain KT2440) GN=rplO PE=3 SV=1
RL15_PSEPW 53 15180 5 (3) 4 (3) 0.54
50S ribosomal protein L15 OS=Pseudomonas putida (strain W619) GN=rplO PE=3 SV=1

-5 peptide matches (4 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
270   560.3433 559.3360 559.3329 5.50 0 35 0.26 +1Score > 42 indicates identity U K.GIAATK.G
727 +1 399.2349 796.4552 796.4483 8.68 0 38 0.034 +1Score > 36 indicates identity U K.FGFVSLK.A
1825   637.3585 1272.7024 1272.6925 7.79 1 55 0.0017 +1Score > 39 indicates identity U K.VEGDVISVQSLK.D
2905   599.3021 1794.8845 1794.8649 10.9 1 26 0.035 +1Score > 40 indicates identity
Score > 24 indicates homology		 U R.SGGSIAPGFEGGQQPLHR.R + Deamidated (NQ)

3 subsets and intersections (14 subset proteins in total)

Score Mass Subset of
RL15_PSEU5 40 15133 91.1
50S ribosomal protein L15 OS=Pseudomonas stutzeri (strain A1501) GN=rplO PE=3 SV=1
RL15_BUCBP 38 16532 91.1
50S ribosomal protein L15 OS=Buchnera aphidicola subsp. Baizongia pistaciae GN=rplO PE=3 SV=1
10 samesets of RL15_BUCBP
RL15_PSE14 38 15157
50S ribosomal protein L15 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplO PE=3 SV=1
RL15_PSEA7 38 15165
50S ribosomal protein L15 OS=Pseudomonas aeruginosa (strain PA7) GN=rplO PE=3 SV=1
RL15_PSEA8 38 15165
50S ribosomal protein L15 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplO PE=3 SV=1
RL15_PSEAB 38 15165
50S ribosomal protein L15 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplO PE=3 SV=1
RL15_PSEAE 38 15165
50S ribosomal protein L15 OS=Pseudomonas aeruginosa GN=rplO PE=3 SV=1
RL15_PSEF5 38 15171
50S ribosomal protein L15 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplO PE=3 SV=1
RL15_PSESM 38 15157
50S ribosomal protein L15 OS=Pseudomonas syringae pv. tomato GN=rplO PE=3 SV=1
RL15_PSEU2 38 15157
50S ribosomal protein L15 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplO PE=3 SV=1
RL15_PSEFS 38 15215
50S ribosomal protein L15 OS=Pseudomonas fluorescens (strain SBW25) GN=rplO PE=3 SV=1
RL15_PSEPF 38 15155
50S ribosomal protein L15 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplO PE=3 SV=1
RL15_PSEE4 34 15165 91.1
50S ribosomal protein L15 OS=Pseudomonas entomophila (strain L48) GN=rplO PE=3 SV=1
1 sameset of RL15_PSEE4
RL15_PSEMY 34 15052
50S ribosomal protein L15 OS=Pseudomonas mendocina (strain ymp) GN=rplO PE=3 SV=1

+92

Accession Score Description
1 RS3_PSEE4 53 30S ribosomal protein S3 OS=Pseudomonas entomophila (strain L48) GN=rpsC PE=3 SV=1

+93

Accession Score Description
1 XIRP1_CHICK 50 Xin actin-binding repeat-containing protein 1 OS=Gallus gallus GN=Xirp1 PE=2 SV=3

+94

Accession Score Description
1 ODP1_PSEAE 50 Pyruvate dehydrogenase E1 component OS=Pseudomonas aeruginosa GN=aceE PE=3 SV=2

+95

Accession Score Description
1 PURA_PSEFS 49 Adenylosuccinate synthetase OS=Pseudomonas fluorescens (strain SBW25) GN=purA PE=3 SV=1

+96

Accession Score Description
1 DNAB_MYCPN 49 Replicative DNA helicase OS=Mycoplasma pneumoniae GN=dnaB PE=1 SV=1

+97

Accession Score Description
1 BETI_PROMH 48 HTH-type transcriptional regulator betI OS=Proteus mirabilis (strain HI4320) GN=betI PE=3 SV=1

+98

Accession Score Description
1 Y396_PSEPK 48 UPF0229 protein PP_0396 OS=Pseudomonas putida (strain KT2440) GN=PP_0396 PE=3 SV=1

+99

Accession Score Description
1 ISPD_ALKMQ 48 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase OS=Alkaliphilus metalliredigens (strain QYMF) GN=ispD PE=3 SV=1

+100

Accession Score Description
1 ARGJ_OCEIH 48 Arginine biosynthesis bifunctional protein argJ OS=Oceanobacillus iheyensis GN=argJ PE=3 SV=1
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