MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 179)

Page: 1 2 3 4 5 6 … 18 Next 

+1

Accession Score Description
Family member distances as a dendrogram 1 EFTU2_PSEPK 1121 Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1
6 EFTU_CYAP7 40 Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1
5 EFTU_ANATD 50 Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1
3 EFTU_PSEFS 701 Elongation factor Tu OS=Pseudomonas fluorescens (strain SBW25) GN=tuf PE=3 SV=1
2 EFTU1_PSEPK 1002 Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1
4 EFTU_CARRP 145 Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1

+2

Accession Score Description
1 ATPB_PSEPG 972 ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1

+3

Accession Score Description
Family member distances as a dendrogram 1 ATPA_PSEPK 749 ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1
2 ATPA_RICAH 179 ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1
3 ATPA_VEREI 174 ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1

+4

Accession Score Description
Family member distances as a dendrogram 1 CH60_PSEPK 584 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1
3 CH602_SORC5 80 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1
2 CH601_ECOK1 123 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1

+5

Accession Score Description
1 DLDH2_PSEPU 541 Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4

+6

Accession Score Description
1 RL1_PSEE4 419 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1

+7

Accession Score Description
1 EFG1_PSEPK 337 Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1

+8

Accession Score Description
1 RS5_PSEE4 331 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1

-9

Accession Score Description
Family member distances as a dendrogram 1 RL4_PSE14 327 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1
2 RL4_PSEE4 318 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
RL4_PSE14 327 21901 18 (9) 6 (5) 0.84
50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1
2 samesets of RL4_PSE14
RL4_PSESM 327 21928 18 (9) 6 (5) 0.83
50S ribosomal protein L4 OS=Pseudomonas syringae pv. tomato GN=rplD PE=3 SV=1
RL4_PSEU2 327 21928 18 (9) 6 (5) 0.83
50S ribosomal protein L4 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplD PE=3 SV=1
RL4_PSEE4 318 21825 21 (9) 8 (5) 0.66
50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1
4 samesets of RL4_PSEE4
RL4_PSEP1 318 21798 21 (9) 8 (5) 0.66
50S ribosomal protein L4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplD PE=3 SV=1
RL4_PSEPG 318 21798 21 (9) 8 (5) 0.66
50S ribosomal protein L4 OS=Pseudomonas putida (strain GB-1) GN=rplD PE=3 SV=1
RL4_PSEPK 318 21798 21 (9) 8 (5) 0.66
50S ribosomal protein L4 OS=Pseudomonas putida (strain KT2440) GN=rplD PE=3 SV=1
RL4_PSEPW 318 21798 21 (9) 8 (5) 0.66
50S ribosomal protein L4 OS=Pseudomonas putida (strain W619) GN=rplD PE=3 SV=1

-23 peptide matches (13 non-duplicate, 10 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
355 +1 314.6832 627.3518 627.3493 4.10 0 31 0.12 +1Score > 34 indicates identity
Score > 34 indicates homology		 X R.GPIWR.G
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_2_20250714115216.raw

Score > 37 indicates identity
965   900.5583 899.5510 899.5440 7.82 1 36 0.075 -1Score > 37 indicates identity U X X R.SILAELVR  
-4.68 1 29 0.38 2 KIQGSLVR  
-12.9 0 24 1.1 3 CILLLLR  
7.84 0 24 1.1 4 NLASLLLR   + Deamidated (NQ)
7.82 0 18 4.2 5 ISLAIVQR   + Deamidated (NQ)
7.82 1 18 4.2 5 LSLAVIER  
-4.67 1 18 4.3 7 VTIAAAKAR  
-4.67 1 17 6.2 8 AINKSIVR  
15.1 1 17 6.2 9 MRIPVIR   + Oxidation (M)
7.82 1 16 7.1 10 EIASLVIR  
966 +2 450.7837 899.5528 899.5440 9.85 1 54 0.0013 +1Score > 37 indicates identity U X X R.SILAELVR  
1055 +2 465.3075 928.6004 928.5957 5.09 0 41 0.0053 +1Score > 30 indicates identity U X X K.VLITVSAVK.K
1111 +1 475.2634 948.5122 948.5141 -1.97 0 11 1.9 +3Score > 41 indicates identity
Score > 27 indicates homology		 U X X R.NLPHVDVR.D
1114 +1 317.1797 948.5173 948.5141 3.33 0 32 0.016 +1Score > 40 indicates identity
Score > 27 indicates homology		 U X X R.NLPHVDVR.D
2133 +2 707.9083 1413.8020 1413.7868 10.8 1 93 4.9e-008 +1Score > 37 indicates identity
Score > 32 indicates homology		 U X X R.LVVVQDFAVEAPK.T
2139   708.4031 1414.7916 1414.7708 14.7 1 8 0.39 +1Score > 38 indicates identity
Score > 17 indicates homology		 U X X R.LVVVQDFAVEAPK.T + Deamidated (NQ)
2573 +1 810.9161 1619.8176 1619.8043 8.27 1 88 3.4e-008 +1Score > 40 indicates identity
Score > 26 indicates homology		 U X R.DVQGSDPVSLIAYEK.V
2646   414.9636 1655.8253 1655.8016 14.3 0 3 1.9 +3Score > 40 indicates identity
Score > 18 indicates homology		 U X R.GGGVTFAARPQDHSQK.L + Deamidated (NQ)
3165   660.9836 1979.9290 1979.9411 -6.13 1 3 1.8 +4Score > 39 indicates identity
Score > 18 indicates homology		 U X X E.FNETLVHQAVVAYMAGGR.Q + 2 Deamidated (NQ); Oxidation (M)
4038   883.4792 2647.4158 2647.3891 10.1 0 117 2.4e-011 +1Score > 34 indicates identity
Score > 24 indicates homology		 U X K.LTGMGLTDVLIVSDAVDQNLYLAAR.N
4042   883.8085 2648.4037 2648.3731 11.5 0 60 1.8e-005 +1Score > 35 indicates identity
Score > 26 indicates homology		 U X K.LTGMGLTDVLIVSDAVDQNLYLAAR.N + Deamidated (NQ)

4 subsets and intersections (10 subset proteins in total)

Score Mass Subset of
RL4_PSEMY 170 21543 9.1, 9.2
50S ribosomal protein L4 OS=Pseudomonas mendocina (strain ymp) GN=rplD PE=3 SV=1
RL4_PSEF5 66 21936 9.1
50S ribosomal protein L4 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplD PE=3 SV=1
3 samesets of RL4_PSEF5
RL4_PSEU5 66 21685
50S ribosomal protein L4 OS=Pseudomonas stutzeri (strain A1501) GN=rplD PE=3 SV=1
RL4_PSEFS 66 21987
50S ribosomal protein L4 OS=Pseudomonas fluorescens (strain SBW25) GN=rplD PE=3 SV=1
RL4_PSEPF 66 22005
50S ribosomal protein L4 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplD PE=3 SV=1
RL4_PSEA7 54 21627 9.1
50S ribosomal protein L4 OS=Pseudomonas aeruginosa (strain PA7) GN=rplD PE=3 SV=1
3 samesets of RL4_PSEA7
RL4_PSEA8 54 21600
50S ribosomal protein L4 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplD PE=3 SV=1
RL4_PSEAB 54 21627
50S ribosomal protein L4 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplD PE=3 SV=1
RL4_PSEAE 54 21627
50S ribosomal protein L4 OS=Pseudomonas aeruginosa GN=rplD PE=3 SV=1
RL4_AZOVD 32 21688 9.1
50S ribosomal protein L4 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplD PE=3 SV=1

+10

Accession Score Description
1 RL18_PSEP1 310 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1
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