MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 179)


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+81

Accession Score Description
1 CLPX_PSEMY 66 ATP-dependent Clp protease ATP-binding subunit clpX OS=Pseudomonas mendocina (strain ymp) GN=clpX PE=3 SV=1

+82

Accession Score Description
1 RS15_PSEPG 65 30S ribosomal protein S15 OS=Pseudomonas putida (strain GB-1) GN=rpsO PE=3 SV=1

+83

Accession Score Description
1 ATPE_PSEPG 62 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

+84

Accession Score Description
1 ARPC_PSEPU 62 Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1

+85

Accession Score Description
1 RS18_PSEE4 62 30S ribosomal protein S18 OS=Pseudomonas entomophila (strain L48) GN=rpsR PE=3 SV=1

+86

Accession Score Description
1 RS4_PSEP1 60 30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1

-87

Accession Score Description
1 FLAE_VIBCH 59 Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1
Score Mass Matches Sequences emPAI
87.1 FLAE_VIBCH 59 41017 5 (3) 3 (2) 0.12
Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1
15 samesets of FLAE_VIBCH
FLAE_VIBC3 59 41017 5 (3) 3 (2) 0.12
Flagellin E OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaE PE=3 SV=1
FLAA_VIBPA 59 39825 4 (3) 3 (2) 0.12
Polar flagellin A OS=Vibrio parahaemolyticus GN=flaA PE=3 SV=1
FLAA_VIBAN 59 40087 3 (3) 2 (2) 0.12
Flagellin A OS=Vibrio anguillarum GN=flaA PE=3 SV=1
FLAA_VIBCH 59 40358 3 (3) 2 (2) 0.12
Flagellin A OS=Vibrio cholerae GN=flaA PE=3 SV=1
FLAB_VIBPA 59 40149 3 (3) 2 (2) 0.12
Polar flagellin B/D OS=Vibrio parahaemolyticus GN=flaB PE=3 SV=2
FLAC_VIBPA 59 40787 3 (3) 2 (2) 0.12
Polar flagellin C OS=Vibrio parahaemolyticus GN=flaC PE=3 SV=2
FLAD_VIBAN 59 39617 3 (3) 2 (2) 0.12
Flagellin D OS=Vibrio anguillarum GN=flaD PE=1 SV=3
FLAE_VIBAN 59 40757 3 (3) 2 (2) 0.12
Probable flagellin E OS=Vibrio anguillarum GN=flaE PE=3 SV=1
FLICB_PSEAE 59 49213 3 (3) 2 (2) 0.10
B-type flagellin OS=Pseudomonas aeruginosa GN=fliC PE=1 SV=2
FLAA_VIBC3 59 40358 3 (3) 2 (2) 0.12
Flagellin A OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaA PE=3 SV=1
FLAC_VIBCH 59 39881 3 (3) 2 (2) 0.12
Flagellin C OS=Vibrio cholerae GN=flaC PE=3 SV=1
FLAD_VIBCH 59 39880 3 (3) 2 (2) 0.12
Flagellin D OS=Vibrio cholerae GN=flaD PE=1 SV=1
FLAF_VIBPA 59 40476 3 (3) 2 (2) 0.12
Polar flagellin F OS=Vibrio parahaemolyticus GN=flaF PE=3 SV=2
FLAC_VIBC3 59 39881 3 (3) 2 (2) 0.12
Flagellin C OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaC PE=3 SV=1
FLAD_VIBC3 59 39880 3 (3) 2 (2) 0.12
Flagellin D OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaD PE=3 SV=1

-5 peptide matches (3 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1581 +1 580.2901 1158.5656 1158.5629 2.37 0 48 0.011 +1Score > 41 indicates identity U K.DDAAGLQISNR.L
1677 +1 601.3408 1200.6670 1200.6761 -7.54 1 19 2.6 +5Score > 40 indicates identity
Score > 36 indicates homology
U E.TQMRGLGIAVR.N
2693   558.6287 1672.8643 1672.8380 15.7 1 35 0.0084 +1Score > 40 indicates identity
Score > 27 indicates homology
U R.INSAKDDAAGLQISNR.L + Deamidated (NQ)

1 subset or intersection (4 subset proteins in total)

Score Mass Subset of
FLAB_VIBAN 55 39509 87.1
Flagellin B OS=Vibrio anguillarum GN=flaB PE=1 SV=3
3 samesets of FLAB_VIBAN
FLAB_VIBCH 55 39492
Flagellin B OS=Vibrio cholerae GN=flaB PE=3 SV=1
FLAB_VIBC3 55 39492
Flagellin B OS=Vibrio cholerae serotype O1 (strain ATCC 39541 / Ogawa 395 / O395) GN=flaB PE=3 SV=1
FLAC_VIBAN 55 40076
Flagellin C OS=Vibrio anguillarum GN=flaC PE=1 SV=3

+88

Accession Score Description
1 CYOB_PSEPU 56 Ubiquinol oxidase subunit 1 OS=Pseudomonas putida GN=cyoB PE=3 SV=1

+89

Accession Score Description
1 RL23_PSEE4 56 50S ribosomal protein L23 OS=Pseudomonas entomophila (strain L48) GN=rplW PE=3 SV=1

+90

Accession Score Description
1 EFTS_PSEPK 55 Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1
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