MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 179)


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+81

Accession Score Description
1 CLPX_PSEMY 66 ATP-dependent Clp protease ATP-binding subunit clpX OS=Pseudomonas mendocina (strain ymp) GN=clpX PE=3 SV=1

+82

Accession Score Description
1 RS15_PSEPG 65 30S ribosomal protein S15 OS=Pseudomonas putida (strain GB-1) GN=rpsO PE=3 SV=1

+83

Accession Score Description
1 ATPE_PSEPG 62 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

+84

Accession Score Description
1 ARPC_PSEPU 62 Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1

+85

Accession Score Description
1 RS18_PSEE4 62 30S ribosomal protein S18 OS=Pseudomonas entomophila (strain L48) GN=rpsR PE=3 SV=1

-86

Accession Score Description
1 RS4_PSEP1 60 30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1
Score Mass Matches Sequences emPAI
86.1 RS4_PSEP1 60 23270 11 (2) 7 (2) 0.21
30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1
3 samesets of RS4_PSEP1
RS4_PSEPG 60 23284 11 (2) 7 (2) 0.21
30S ribosomal protein S4 OS=Pseudomonas putida (strain GB-1) GN=rpsD PE=3 SV=1
RS4_PSEPK 60 23270 11 (2) 7 (2) 0.21
30S ribosomal protein S4 OS=Pseudomonas putida (strain KT2440) GN=rpsD PE=3 SV=1
RS4_PSEPW 60 23287 10 (2) 6 (2) 0.21
30S ribosomal protein S4 OS=Pseudomonas putida (strain W619) GN=rpsD PE=3 SV=1

-11 peptide matches (8 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
837 +1 425.2477 848.4808 848.4756 6.20 1 24 2 +8Score > 40 indicates identity R.IYGVLER.Q
1023   461.7473 921.4800 921.4807 -0.75 1 20 0.27 +1Score > 41 indicates identity
Score > 26 indicates homology
R.EGTDLFLK.S
1134   479.7368 957.4590 957.4556 3.64 0 19 0.58 +1Score > 40 indicates identity
Score > 29 indicates homology
U R.GYYQAAASK.K
1384   534.2615 1066.5084 1066.5043 3.86 0 51 0.00058 +1Score > 41 indicates identity
Score > 31 indicates homology
U R.QSDYGTQLR.E
1517   566.2918 1130.5690 1130.5608 7.30 1 5 1.9 +8Score > 41 indicates identity
Score > 21 indicates homology
U R.VEWVDVDAAK.K
1886   650.8602 1299.7058 1299.6969 6.90 1 20 0.44 +1Score > 40 indicates identity
Score > 29 indicates homology
U R.IVQALELCAQR.G
3152 +2 657.3723 1969.0951 1969.0745 10.4 0 20 0.08 +1Score > 35 indicates identity
Score > 21 indicates homology
U K.TVNIPSYQVRPGDVVAVR.E
3155   986.0521 1970.0896 1970.0585 15.8 0 38 0.003 +1Score > 35 indicates identity
Score > 25 indicates homology
U K.TVNIPSYQVRPGDVVAVR.E + Deamidated (NQ)

1 subset or intersection (8 subset proteins in total)

Score Mass Subset of
RS4_PSE14 51 23290 86.1
30S ribosomal protein S4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsD PE=3 SV=1
7 samesets of RS4_PSE14
RS4_PSEE4 51 23361
30S ribosomal protein S4 OS=Pseudomonas entomophila (strain L48) GN=rpsD PE=3 SV=1
RS4_PSEPF 51 23246
30S ribosomal protein S4 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsD PE=3 SV=1
RS4_PSEF5 51 23289
30S ribosomal protein S4 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsD PE=3 SV=1
RS4_PSEFS 51 23290
30S ribosomal protein S4 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsD PE=3 SV=1
RS4_PSEMY 51 23217
30S ribosomal protein S4 OS=Pseudomonas mendocina (strain ymp) GN=rpsD PE=3 SV=1
RS4_PSESM 51 23290
30S ribosomal protein S4 OS=Pseudomonas syringae pv. tomato GN=rpsD PE=3 SV=1
RS4_PSEU2 51 23290
30S ribosomal protein S4 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsD PE=3 SV=1

+87

Accession Score Description
1 FLAE_VIBCH 59 Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1

+88

Accession Score Description
1 CYOB_PSEPU 56 Ubiquinol oxidase subunit 1 OS=Pseudomonas putida GN=cyoB PE=3 SV=1

+89

Accession Score Description
1 RL23_PSEE4 56 50S ribosomal protein L23 OS=Pseudomonas entomophila (strain L48) GN=rplW PE=3 SV=1

+90

Accession Score Description
1 EFTS_PSEPK 55 Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1
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