MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 81–90 (out of 179)


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-81

Accession Score Description
1 CLPX_PSEMY 66 ATP-dependent Clp protease ATP-binding subunit clpX OS=Pseudomonas mendocina (strain ymp) GN=clpX PE=3 SV=1
Score Mass Matches Sequences emPAI
81.1 CLPX_PSEMY 66 47098 7 (1) 5 (1) 0.05
ATP-dependent Clp protease ATP-binding subunit clpX OS=Pseudomonas mendocina (strain ymp) GN=clpX PE=3 SV=1
+627 samesets of CLPX_PSEMY

-7 peptide matches (6 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1204   494.2982 986.5818 986.5760 5.91 1 36 0.068 +1Score > 39 indicates identity
Score > 37 indicates homology
U K.TLLAETLAR.L
1532 +1 567.8247 1133.6348 1133.6233 10.2 1 20 0.7 +1Score > 38 indicates identity
Score > 31 indicates homology
K.FGLIPEFVGR.L
1634   394.9010 1181.6812 1181.7019 -17.6 0 5 2 +4Score > 36 indicates identity
Score > 21 indicates homology
U E.AALVQILTEPK.N
1797   628.8694 1255.7242 1255.7136 8.49 0 35 0.052 +1Score > 38 indicates identity
Score > 35 indicates homology
U K.SNILLIGPTGSGK.T
1973   672.3651 1342.7156 1342.7092 4.77 1 66 3.4e-005 +1Score > 40 indicates identity
Score > 34 indicates homology
U R.DVSGEGVQQALLK.L
1977   672.8505 1343.6864 1343.6933 -5.06 1 14 2.7 +7Score > 41 indicates identity
Score > 30 indicates homology
U R.DVSGEGVQQALLK.L + Deamidated (NQ)

+82

Accession Score Description
1 RS15_PSEPG 65 30S ribosomal protein S15 OS=Pseudomonas putida (strain GB-1) GN=rpsO PE=3 SV=1

+83

Accession Score Description
1 ATPE_PSEPG 62 ATP synthase epsilon chain OS=Pseudomonas putida (strain GB-1) GN=atpC PE=3 SV=1

+84

Accession Score Description
1 ARPC_PSEPU 62 Antibiotic efflux pump outer membrane protein arpC OS=Pseudomonas putida GN=arpC PE=2 SV=1

+85

Accession Score Description
1 RS18_PSEE4 62 30S ribosomal protein S18 OS=Pseudomonas entomophila (strain L48) GN=rpsR PE=3 SV=1

+86

Accession Score Description
1 RS4_PSEP1 60 30S ribosomal protein S4 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsD PE=3 SV=1

+87

Accession Score Description
1 FLAE_VIBCH 59 Flagellin E OS=Vibrio cholerae GN=flaE PE=3 SV=1

+88

Accession Score Description
1 CYOB_PSEPU 56 Ubiquinol oxidase subunit 1 OS=Pseudomonas putida GN=cyoB PE=3 SV=1

+89

Accession Score Description
1 RL23_PSEE4 56 50S ribosomal protein L23 OS=Pseudomonas entomophila (strain L48) GN=rplW PE=3 SV=1

+90

Accession Score Description
1 EFTS_PSEPK 55 Elongation factor Ts OS=Pseudomonas putida (strain KT2440) GN=tsf PE=3 SV=1
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