MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1121 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 6 | EFTU_CYAP7 | 40 | Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1 | |
| 5 | EFTU_ANATD | 50 | Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1 | |
| 3 | EFTU_PSEFS | 701 | Elongation factor Tu OS=Pseudomonas fluorescens (strain SBW25) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1002 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
| 4 | EFTU_CARRP | 145 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 972 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 749 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_RICAH | 179 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_VEREI | 174 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 584 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 3 | CH602_SORC5 | 80 | 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1 | |
| 2 | CH601_ECOK1 | 123 | 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DLDH2_PSEPU | 541 | Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 419 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 337 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 331 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 8.1 | RS5_PSEE4 |
331 | 17700 | 15 (9) | 5 (3) | 0.65 | |
| 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 | |||||||
| 4 samesets of RS5_PSEE4 | |||||||
| RS5_PSEP1 |
331 | 17712 | 15 (9) | 5 (3) | 0.64 | ||
| 30S ribosomal protein S5 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsE PE=3 SV=1 | |||||||
| RS5_PSEPG |
331 | 17712 | 15 (9) | 5 (3) | 0.64 | ||
| 30S ribosomal protein S5 OS=Pseudomonas putida (strain GB-1) GN=rpsE PE=3 SV=1 | |||||||
| RS5_PSEPK |
331 | 17712 | 15 (9) | 5 (3) | 0.64 | ||
| 30S ribosomal protein S5 OS=Pseudomonas putida (strain KT2440) GN=rpsE PE=3 SV=1 | |||||||
| RS5_PSEPW |
331 | 17712 | 15 (9) | 5 (3) | 0.64 | ||
| 30S ribosomal protein S5 OS=Pseudomonas putida (strain W619) GN=rpsE PE=3 SV=1 | |||||||
15 peptide matches (8 non-duplicate, 7 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 573 |
728.4438 | 727.4365 | 727.4341 | 3.38 | 0 | 27 | 0.27 | 1Score > 39 indicates identity Score > 34 indicates homology |
K.LVQVNR.V | ||
| 574 |
1 |
364.7256 | 727.4366 | 727.4341 | 3.55 | 0 | 27 | 0.18 | 1Score > 39 indicates identity Score > 32 indicates homology |
K.LVQVNR.V | |
| 578 |
365.2127 | 728.4108 | 728.4181 | -9.92 | 0 | 22 | 0.95 | 1Score > 42 indicates identity Score > 34 indicates homology |
K.LVQVNR.V + Deamidated (NQ) | ||
| 860 |
1 |
428.2510 | 854.4874 | 854.4861 | 1.52 | 1 | 17 | 0.47 | 1Score > 37 indicates identity Score > 26 indicates homology |
R.EVPAAIQK.A | |
| 1196 |
982.5234 | 981.5161 | 981.5131 | 3.07 | 0 | 51 | 0.0014 | 1Score > 39 indicates identity Score > 34 indicates homology |
U | K.GTTLQYATK.A | |
| 1198 |
1 |
491.7656 | 981.5166 | 981.5131 | 3.61 | 0 | 38 | 0.028 | 1Score > 39 indicates identity Score > 35 indicates homology |
U | K.GTTLQYATK.A |
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_2_20250714115216.rawScore > 32 indicates identity Score > 30 indicates homology |
|||||||||||
| 2125 |
2 |
705.9284 | 1409.8422 | 1409.8242 | 12.8 | 1 | 64 | 2.3e-005 | 1Score > 32 indicates identity Score > 30 indicates homology |
U | R.AVLEVAGVQNVLAK.C |
| -5.03 | 1 | 13 | 2.5 | 2 | GLIELADLLVINK | ||||||
| -3.13 | 1 | 8 | 8.5 | 3 | IGLIQRVSQAAVR | ||||||
| 12.8 | 1 | 8 | 9.6 | 4 | GLLDAIVPRNTLK + Deamidated (NQ) | ||||||
| -18.2 | 1 | 8 | 9.6 | 4 | VALIANIPVIKMK + Deamidated (NQ) | ||||||
| 12.8 | 1 | 7 | 10 | 6 | AVLANLLLAVAEGR + Deamidated (NQ) | ||||||
| 12.8 | 1 | 7 | 11 | 7 | SLLVVKGNNIPGAK + Deamidated (NQ) | ||||||
| 7.56 | 1 | 7 | 12 | 8 | AVLMKEPPVVALK + Oxidation (M) | ||||||
| 9.94 | 1 | 7 | 12 | 8 | AVELGVLPGPLFAK | ||||||
| 1.96 | 0 | 6 | 13 | 10 | AVLLFVVQHSGIK | ||||||
| 2230 |
2 |
734.9164 | 1467.8182 | 1467.7974 | 14.2 | 0 | 105 | 8.3e-009 | 1Score > 37 indicates identity Score > 37 indicates homology |
U | R.IFTFTALTVVGDGK.G |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| RS5_AZOVD |
303 | 17763 | 8.1 | ||
| 30S ribosomal protein S5 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsE PE=3 SV=1 | |||||
| 7 samesets of RS5_AZOVD | |||||
| RS5_PSE14 |
303 | 17881 | |||
| 30S ribosomal protein S5 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEF5 |
303 | 17699 | |||
| 30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEFS |
303 | 17732 | |||
| 30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEMY |
303 | 17708 | |||
| 30S ribosomal protein S5 OS=Pseudomonas mendocina (strain ymp) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEPF |
303 | 17769 | |||
| 30S ribosomal protein S5 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSESM |
303 | 17778 | |||
| 30S ribosomal protein S5 OS=Pseudomonas syringae pv. tomato GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEU2 |
303 | 17778 | |||
| 30S ribosomal protein S5 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEU5 |
232 | 17649 | 8.1 | ||
| 30S ribosomal protein S5 OS=Pseudomonas stutzeri (strain A1501) GN=rpsE PE=3 SV=1 | |||||
| RS5_ALCBS |
101 | 17601 | 8.1 | ||
| 30S ribosomal protein S5 OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpsE PE=3 SV=1 | |||||
| 5 samesets of RS5_ALCBS | |||||
| RS5_PSEA7 |
101 | 17671 | |||
| 30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEA8 |
101 | 17671 | |||
| 30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEAB |
101 | 17671 | |||
| 30S ribosomal protein S5 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsE PE=3 SV=1 | |||||
| RS5_PSEAE |
101 | 17671 | |||
| 30S ribosomal protein S5 OS=Pseudomonas aeruginosa GN=rpsE PE=3 SV=1 | |||||
| RS5_HAHCH |
101 | 17639 | |||
| 30S ribosomal protein S5 OS=Hahella chejuensis (strain KCTC 2396) GN=rpsE PE=3 SV=1 | |||||
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_PSE14 | 327 | 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1 | |
| 2 | RL4_PSEE4 | 318 | 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1 | |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEP1 | 310 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1 |
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