MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 179)

Page: Previous 1 …3 4 5 6 7 8 9 10 11 12 13 … 18 Next 

+71

Accession Score Description
1 RL16_PSEF5 74 50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplP PE=3 SV=1

+72

Accession Score Description
1 RS7_PSEE4 73 30S ribosomal protein S7 OS=Pseudomonas entomophila (strain L48) GN=rpsG PE=3 SV=1

+73

Accession Score Description
1 CISY_PSEAE 73 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

+74

Accession Score Description
1 SUCD_PSEAE 71 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

-75

Accession Score Description
1 RL13_PSEP1 69 50S ribosomal protein L13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplM PE=3 SV=1
Score Mass Matches Sequences emPAI
75.1 RL13_PSEP1 69 15852 6 (2) 4 (2) 0.32
50S ribosomal protein L13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplM PE=3 SV=1
2 samesets of RL13_PSEP1
RL13_PSEPG 69 15852 6 (2) 4 (2) 0.32
50S ribosomal protein L13 OS=Pseudomonas putida (strain GB-1) GN=rplM PE=3 SV=1
RL13_PSEPK 69 15852 6 (2) 4 (2) 0.32
50S ribosomal protein L13 OS=Pseudomonas putida (strain KT2440) GN=rplM PE=3 SV=1

-6 peptide matches (4 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
630   380.2354 758.4562 758.4538 3.23 1 22 1.3 +2Score > 40 indicates identity
Score > 36 indicates homology		 R.VIETAVK.G
907 +2 437.7550 873.4954 873.4920 3.99 1 37 0.052 +1Score > 42 indicates identity
Score > 37 indicates homology		 U R.LATEIATR.L
2396   774.9025 1547.7904 1547.7733 11.1 1 71 5.4e-005 +1Score > 41 indicates identity
Score > 41 indicates homology		 U R.EWFVVDAAGQTLGR.L
3126   487.2545 1944.9889 1944.9806 4.27 1 3 1.8 +6Score > 40 indicates identity
Score > 18 indicates homology		 K.VYAGAAHPHTAQQPQELK.I

2 subsets and intersections (11 subset proteins in total)

Score Mass Subset of
RL13_PSEE4 71 15838 75.1
50S ribosomal protein L13 OS=Pseudomonas entomophila (strain L48) GN=rplM PE=3 SV=1
1 sameset of RL13_PSEE4
RL13_PSEPW 71 15852
50S ribosomal protein L13 OS=Pseudomonas putida (strain W619) GN=rplM PE=3 SV=1
RL13_COLP3 37 15993 75.1
50S ribosomal protein L13 OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=rplM PE=3 SV=1
8 samesets of RL13_COLP3
RL13_PSYA2 37 15834
50S ribosomal protein L13 OS=Psychrobacter arcticus (strain DSM 17307 / 273-4) GN=rplM PE=3 SV=1
RL13_PSYCK 37 15701
50S ribosomal protein L13 OS=Psychrobacter cryohalolentis (strain K5) GN=rplM PE=3 SV=1
RL13_SHEB2 37 15728
50S ribosomal protein L13 OS=Shewanella baltica (strain OS223) GN=rplM PE=3 SV=1
RL13_SHEB5 37 15728
50S ribosomal protein L13 OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=rplM PE=3 SV=1
RL13_SHEB9 37 15728
50S ribosomal protein L13 OS=Shewanella baltica (strain OS195) GN=rplM PE=3 SV=1
RL13_SHEFN 37 15736
50S ribosomal protein L13 OS=Shewanella frigidimarina (strain NCIMB 400) GN=rplM PE=3 SV=1
RL13_SHEPC 37 15721
50S ribosomal protein L13 OS=Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) GN=rplM PE=3 SV=1
RL13_SHESW 37 15721
50S ribosomal protein L13 OS=Shewanella sp. (strain W3-18-1) GN=rplM PE=3 SV=1

+76

Accession Score Description
1 HEMN_PSEAE 71 Oxygen-independent coproporphyrinogen-III oxidase OS=Pseudomonas aeruginosa GN=hemN PE=3 SV=2

+77

Accession Score Description
1 YJJK_ECOLI 69 Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2

+78

Accession Score Description
1 ODBB_PSEPU 69 2-oxoisovalerate dehydrogenase subunit beta OS=Pseudomonas putida GN=bkdA2 PE=1 SV=1

+79

Accession Score Description
1 ENO_HAES1 67 Enolase OS=Haemophilus somnus (strain 129Pt) GN=eno PE=3 SV=1

+80

Accession Score Description
1 GLNA_AZOVI 67 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1
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