MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 71–80 (out of 179)


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-71

Accession Score Description
1 RL16_PSEF5 74 50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplP PE=3 SV=1
Score Mass Matches Sequences emPAI
71.1 RL16_PSEF5 74 15407 7 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplP PE=3 SV=1
11 samesets of RL16_PSEF5
RL16_PSEFS 74 15422 7 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain SBW25) GN=rplP PE=3 SV=1
RL16_PSEP1 74 15364 7 (5) 4 (3) 0.54
50S ribosomal protein L16 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplP PE=3 SV=1
RL16_PSEPF 74 15407 7 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplP PE=3 SV=1
RL16_PSEPG 74 15364 7 (5) 4 (3) 0.54
50S ribosomal protein L16 OS=Pseudomonas putida (strain GB-1) GN=rplP PE=3 SV=1
RL16_PSEPK 74 15364 7 (5) 4 (3) 0.54
50S ribosomal protein L16 OS=Pseudomonas putida (strain KT2440) GN=rplP PE=3 SV=1
RL16_PSEPW 74 15364 7 (5) 4 (3) 0.54
50S ribosomal protein L16 OS=Pseudomonas putida (strain W619) GN=rplP PE=3 SV=1
RL16_PSE14 74 15421 7 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplP PE=3 SV=1
RL16_PSEE4 74 15364 7 (5) 4 (3) 0.54
50S ribosomal protein L16 OS=Pseudomonas entomophila (strain L48) GN=rplP PE=3 SV=1
RL16_PSESM 74 15421 7 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas syringae pv. tomato GN=rplP PE=3 SV=1
RL16_PSEU2 74 15421 7 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplP PE=3 SV=1
RL16_PSEU5 74 15395 7 (5) 4 (3) 0.53
50S ribosomal protein L16 OS=Pseudomonas stutzeri (strain A1501) GN=rplP PE=3 SV=1

+7 peptide matches (4 non-duplicate, 3 duplicate)

4 subsets and intersections (37 subset proteins in total)

Score Mass Subset of
RL16_PSEA7 56 15362 71.1
50S ribosomal protein L16 OS=Pseudomonas aeruginosa (strain PA7) GN=rplP PE=3 SV=1
3 samesets of RL16_PSEA7
RL16_PSEA8 56 15392
50S ribosomal protein L16 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplP PE=3 SV=1
RL16_PSEAB 56 15392
50S ribosomal protein L16 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplP PE=3 SV=1
RL16_PSEAE 56 15392
50S ribosomal protein L16 OS=Pseudomonas aeruginosa GN=rplP PE=3 SV=1
RL16_AZOVD 52 15445 71.1
50S ribosomal protein L16 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplP PE=3 SV=1
1 sameset of RL16_AZOVD
RL16_PSEMY 52 15379
50S ribosomal protein L16 OS=Pseudomonas mendocina (strain ymp) GN=rplP PE=3 SV=1
RL16_SACD2 47 15613 71.1
50S ribosomal protein L16 OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rplP PE=3 SV=1
RL16_ACIB3 40 15514 71.1
50S ribosomal protein L16 OS=Acinetobacter baumannii (strain AB307-0294) GN=rplP PE=3 SV=1
+29 samesets of RL16_ACIB3

+72

Accession Score Description
1 RS7_PSEE4 73 30S ribosomal protein S7 OS=Pseudomonas entomophila (strain L48) GN=rpsG PE=3 SV=1

+73

Accession Score Description
1 CISY_PSEAE 73 Citrate synthase OS=Pseudomonas aeruginosa GN=gltA PE=3 SV=2

+74

Accession Score Description
1 SUCD_PSEAE 71 Succinyl-CoA ligase [ADP-forming] subunit alpha OS=Pseudomonas aeruginosa GN=sucD PE=3 SV=2

+75

Accession Score Description
1 RL13_PSEP1 69 50S ribosomal protein L13 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplM PE=3 SV=1

+76

Accession Score Description
1 HEMN_PSEAE 71 Oxygen-independent coproporphyrinogen-III oxidase OS=Pseudomonas aeruginosa GN=hemN PE=3 SV=2

+77

Accession Score Description
1 YJJK_ECOLI 69 Uncharacterized ABC transporter ATP-binding protein yjjK OS=Escherichia coli (strain K12) GN=yjjK PE=1 SV=2

+78

Accession Score Description
1 ODBB_PSEPU 69 2-oxoisovalerate dehydrogenase subunit beta OS=Pseudomonas putida GN=bkdA2 PE=1 SV=1

+79

Accession Score Description
1 ENO_HAES1 67 Enolase OS=Haemophilus somnus (strain 129Pt) GN=eno PE=3 SV=1

+80

Accession Score Description
1 GLNA_AZOVI 67 Glutamine synthetase OS=Azotobacter vinelandii GN=glnA PE=3 SV=1
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