MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1121 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 6 | EFTU_CYAP7 | 40 | Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1 | |
| 5 | EFTU_ANATD | 50 | Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1 | |
| 3 | EFTU_PSEFS | 701 | Elongation factor Tu OS=Pseudomonas fluorescens (strain SBW25) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1002 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
| 4 | EFTU_CARRP | 145 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 972 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 749 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_RICAH | 179 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_VEREI | 174 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 584 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 3 | CH602_SORC5 | 80 | 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1 | |
| 2 | CH601_ECOK1 | 123 | 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DLDH2_PSEPU | 541 | Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 419 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 337 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 7.1 | EFG1_PSEPK |
337 | 79053 | 12 (8) | 10 (6) | 0.19 | |
| Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 | |||||||
12 peptide matches (10 non-duplicate, 2 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 553 |
361.2171 | 720.4196 | 720.4170 | 3.64 | 0 | 8 | 1 | 2Score > 38 indicates identity Score > 21 indicates homology |
R.VVYVNK.M | ||
| 611 |
374.7111 | 747.4076 | 747.4028 | 6.55 | 0 | 41 | 0.058 | 1Score > 41 indicates identity |
U | R.AGANFLR.V | |
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_2_20250714115216.rawScore > 42 indicates identity Score > 39 indicates homology |
|||||||||||
| 1378 |
531.7665 | 1061.5184 | 1061.5142 | 4.04 | 1 | 40 | 0.042 | 1Score > 42 indicates identity Score > 39 indicates homology |
U | K.LAQEDPSFR.V | |
| 4.05 | 0 | 24 | 1.7 | 2 | AIQQDYPAR + Deamidated (NQ) | ||||||
| 18.4 | 0 | 24 | 1.8 | 3 | LASVANQSDR + 2 Deamidated (NQ) | ||||||
| -15.8 | 1 | 22 | 3.2 | 4 | IQAQNSKSGK + 2 Deamidated (NQ) | ||||||
| 14.6 | 1 | 22 | 3.2 | 4 | LQAENPNFK + 2 Deamidated (NQ) | ||||||
| -5.26 | 1 | 16 | 13 | 6 | LAKQTNASVE + 2 Deamidated (NQ) | ||||||
| -19.6 | 1 | 16 | 13 | 6 | LAQELVFNE | ||||||
| 4.05 | 0 | 14 | 18 | 8 | AIQQDYPAR + Deamidated (NQ) | ||||||
| 18.4 | 0 | 14 | 19 | 9 | LLNTNNSQR + 3 Deamidated (NQ) | ||||||
| 0.88 | 0 | 14 | 20 | 10 | IAQSDQIMR + Deamidated (NQ) | ||||||
| 1625 |
393.5599 | 1177.6579 | 1177.6495 | 7.08 | 0 | 32 | 0.16 | 1Score > 37 indicates identity |
R.ILFYTGLSHK.M | ||
| 1887 |
650.8937 | 1299.7728 | 1299.7551 | 13.7 | 0 | 37 | 0.00058 | 1Score > 34 indicates identity Score > 17 indicates homology |
U | K.NGVVAGYPLIGLK.A | |
| 2277 |
748.4183 | 1494.8220 | 1494.8082 | 9.24 | 0 | 74 | 1.2e-005 | 1Score > 37 indicates identity |
U | R.GITITSAAVTTFWK.G | |
| 2377 |
1 |
768.9356 | 1535.8566 | 1535.8348 | 14.2 | 0 | 91 | 1.7e-007 | 1Score > 36 indicates identity |
U | K.IATDPFVGTLTFVR.V |
| 2431 |
521.5819 | 1561.7239 | 1561.7049 | 12.2 | 0 | 15 | 0.11 | 1Score > 40 indicates identity Score > 18 indicates homology |
U | R.HADDDEPFSALAFK.I | |
| 2866 |
886.9666 | 1771.9186 | 1771.8992 | 10.9 | 0 | 87 | 1.7e-007 | 1Score > 40 indicates identity Score > 32 indicates homology |
U | R.VYSGFLTSGDSVINSVK.G | |
| 4142 |
1 |
926.1719 | 2775.4939 | 2775.4443 | 17.9 | 1 | 76 | 9e-008 | 1Score > 33 indicates identity Score > 19 indicates homology |
U | R.LGHTPVPVQLAIGSEDNFQGQVDLIK.M + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| EFG_PSEF5 |
185 | 78977 | 7.1 | ||
| Elongation factor G OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=fusA PE=3 SV=1 | |||||
| EFG_PSE14 |
147 | 77465 | 7.1 | ||
| Elongation factor G OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=fusA PE=3 SV=1 | |||||
| 5 samesets of EFG_PSE14 | |||||
| EFG_PSEU2 |
147 | 77378 | |||
| Elongation factor G OS=Pseudomonas syringae pv. syringae (strain B728a) GN=fusA PE=3 SV=1 | |||||
| EFG_PSEMY |
147 | 79128 | |||
| Elongation factor G OS=Pseudomonas mendocina (strain ymp) GN=fusA PE=3 SV=1 | |||||
| EFG_PSEFS |
147 | 77399 | |||
| Elongation factor G OS=Pseudomonas fluorescens (strain SBW25) GN=fusA PE=3 SV=1 | |||||
| EFG_PSEPF |
147 | 77489 | |||
| Elongation factor G OS=Pseudomonas fluorescens (strain Pf0-1) GN=fusA PE=3 SV=1 | |||||
| EFG_PSESM |
147 | 77406 | |||
| Elongation factor G OS=Pseudomonas syringae pv. tomato GN=fusA PE=3 SV=1 | |||||
| EFG_DICNV |
131 | 77910 | 7.1 | ||
| Elongation factor G OS=Dichelobacter nodosus (strain VCS1703A) GN=fusA PE=3 SV=1 | |||||
| EFG_IDILO |
129 | 78421 | 7.1 | ||
| Elongation factor G OS=Idiomarina loihiensis GN=fusA PE=3 SV=1 | |||||
| EFG_ALCBS |
93 | 77664 | 7.1 | ||
| Elongation factor G OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=fusA PE=3 SV=1 | |||||
| EFG1_VIBCH |
90 | 77164 | 7.1 | ||
| Elongation factor G 1 OS=Vibrio cholerae GN=fusA1 PE=3 SV=1 | |||||
| EFG1_PSEAE |
90 | 78077 | 7.1 | ||
| Elongation factor G 1 OS=Pseudomonas aeruginosa GN=fusA PE=3 SV=1 | |||||
| EFG_PSEU5 |
50 | 78200 | 7.1 | ||
| Elongation factor G OS=Pseudomonas stutzeri (strain A1501) GN=fusA PE=3 SV=1 | |||||
| EFG1_BORA1 |
40 | 77364 | 7.1 | ||
| Elongation factor G 1 OS=Bordetella avium (strain 197N) GN=fusA1 PE=3 SV=1 | |||||
| 100 samesets of EFG1_BORA1 |
|||||
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 331 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_PSE14 | 327 | 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1 | |
| 2 | RL4_PSEE4 | 318 | 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1 | |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEP1 | 310 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1 |
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