MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 179)


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+61

Accession Score Description
1 RL28_PSEE4 85 50S ribosomal protein L28 OS=Pseudomonas entomophila (strain L48) GN=rpmB PE=3 SV=1

+62

Accession Score Description
1 CLPB_PSEPK 84 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+63

Accession Score Description
1 ODO1_AZOVI 84 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+64

Accession Score Description
1 KKA2_KLEPN 80 Aminoglycoside 3'-phosphotransferase OS=Klebsiella pneumoniae GN=neo PE=1 SV=1

+65

Accession Score Description
1 YEAG_ECOLI 79 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+66

Accession Score Description
1 RL14_AZOSE 78 50S ribosomal protein L14 OS=Azoarcus sp. (strain EbN1) GN=rplN PE=3 SV=1

+67

Accession Score Description
1 RL29_PSE14 77 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

+68

Accession Score Description
1 OPRI_PSEAE 77 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

-69

Accession Score Description
1 ILVC_PSEE4 75 Ketol-acid reductoisomerase OS=Pseudomonas entomophila (strain L48) GN=ilvC PE=3 SV=1
Score Mass Matches Sequences emPAI
69.1 ILVC_PSEE4 75 36674 3 (2) 3 (2) 0.13
Ketol-acid reductoisomerase OS=Pseudomonas entomophila (strain L48) GN=ilvC PE=3 SV=1
4 samesets of ILVC_PSEE4
ILVC_PSEPG 75 36575 2 (2) 2 (2) 0.13
Ketol-acid reductoisomerase OS=Pseudomonas putida (strain GB-1) GN=ilvC PE=3 SV=1
ILVC_PSEPK 75 36575 2 (2) 2 (2) 0.13
Ketol-acid reductoisomerase OS=Pseudomonas putida (strain KT2440) GN=ilvC PE=3 SV=1
ILVC_PSEPW 75 36561 2 (2) 2 (2) 0.13
Ketol-acid reductoisomerase OS=Pseudomonas putida (strain W619) GN=ilvC PE=3 SV=1
ILVC_PSEP1 75 36575 2 (2) 2 (2) 0.13
Ketol-acid reductoisomerase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ilvC PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1165   971.4993 970.4920 970.4971 -5.22 1 4 2.1 +6Score > 40 indicates identity
Score > 20 indicates homology
U K.QEIEPNIK.K + Deamidated (NQ)
1896   654.3400 1306.6654 1306.6630 1.90 0 72 1.4e-005 +1Score > 41 indicates identity
Score > 36 indicates homology
U K.NVALSYASGVGGGR.T
3013   930.9891 1859.9636 1859.9265 20.0 0 32 0.007 +1Score > 39 indicates identity
Score > 23 indicates homology
U K.GGGIPDLIAIYQDASGNAK.N + Deamidated (NQ)

2 subsets and intersections (18 subset proteins in total)

Score Mass Subset of
ILVC_ACIAD 72 37091 69.1
Ketol-acid reductoisomerase OS=Acinetobacter sp. (strain ADP1) GN=ilvC PE=3 SV=1
10 samesets of ILVC_ACIAD
ILVC_ACIBC 72 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain ACICU) GN=ilvC PE=3 SV=1
ILVC_ACIBS 72 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain SDF) GN=ilvC PE=3 SV=1
ILVC_ACIBT 72 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) GN=ilvC PE=3 SV=2
ILVC_ACIBY 72 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain AYE) GN=ilvC PE=3 SV=1
ILVC_ACIB3 72 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain AB307-0294) GN=ilvC PE=3 SV=1
ILVC_ACIB5 72 37001
Ketol-acid reductoisomerase OS=Acinetobacter baumannii (strain AB0057) GN=ilvC PE=3 SV=1
ILVC_PSEMY 72 36501
Ketol-acid reductoisomerase OS=Pseudomonas mendocina (strain ymp) GN=ilvC PE=3 SV=1
ILVC_PSESM 72 36589
Ketol-acid reductoisomerase OS=Pseudomonas syringae pv. tomato GN=ilvC PE=3 SV=1
ILVC_PSEU2 72 36531
Ketol-acid reductoisomerase OS=Pseudomonas syringae pv. syringae (strain B728a) GN=ilvC PE=3 SV=1
ILVC_PSE14 72 36531
Ketol-acid reductoisomerase OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=ilvC PE=3 SV=1
ILVC_PSEA7 32 36729 69.1
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa (strain PA7) GN=ilvC PE=3 SV=1
6 samesets of ILVC_PSEA7
ILVC_PSEAB 32 36743
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=ilvC PE=3 SV=1
ILVC_PSEAE 32 36743
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa GN=ilvC PE=1 SV=1
ILVC_PSEFS 32 36416
Ketol-acid reductoisomerase OS=Pseudomonas fluorescens (strain SBW25) GN=ilvC PE=3 SV=1
ILVC_PSEPF 32 36441
Ketol-acid reductoisomerase OS=Pseudomonas fluorescens (strain Pf0-1) GN=ilvC PE=3 SV=1
ILVC_PSEF5 32 36441
Ketol-acid reductoisomerase OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=ilvC PE=3 SV=1
ILVC_PSEA8 32 36743
Ketol-acid reductoisomerase OS=Pseudomonas aeruginosa (strain LESB58) GN=ilvC PE=3 SV=1

+70

Accession Score Description
1 CH10_PSE14 75 10 kDa chaperonin OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=groS PE=3 SV=1
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