MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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61| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL28_PSEE4 | 85 | 50S ribosomal protein L28 OS=Pseudomonas entomophila (strain L48) GN=rpmB PE=3 SV=1 |
62| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CLPB_PSEPK | 84 | Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1 |
63| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODO1_AZOVI | 84 | 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1 |
64| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | KKA2_KLEPN | 80 | Aminoglycoside 3'-phosphotransferase OS=Klebsiella pneumoniae GN=neo PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 64.1 | KKA2_KLEPN |
80 | 29315 | 10 (3) | 6 (2) | 0.26 | |
| Aminoglycoside 3'-phosphotransferase OS=Klebsiella pneumoniae GN=neo PE=1 SV=1 | |||||||
10 peptide matches (7 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 361 |
315.6831 | 629.3516 | 629.3497 | 3.14 | 0 | 37 | 0.055 | 1Score > 43 indicates identity Score > 37 indicates homology |
U | R.LGVADR.Y | |
| 418 |
1 |
335.1909 | 668.3672 | 668.3646 | 3.99 | 0 | 13 | 0.76 | 1Score > 34 indicates identity Score > 25 indicates homology |
R.IAFYR.L | |
| 693 |
1 |
783.4016 | 782.3943 | 782.3851 | 11.8 | 1 | 10 | 0.28 | 2Score > 39 indicates identity Score > 17 indicates homology |
U | R.LLDEFF.- |
| 1355 |
1 |
525.7847 | 1049.5548 | 1049.5505 | 4.11 | 0 | 39 | 0.082 | 1Score > 41 indicates identity |
U | R.YQDIALATR.D |
| 1908 |
438.9367 | 1313.7883 | 1313.7820 | 4.81 | 0 | 22 | 0.24 | 1Score > 33 indicates identity Score > 28 indicates homology |
U | R.LSAQGRPVLFVK.T | |
| 2401 |
517.2906 | 1548.8500 | 1548.8300 | 12.9 | 0 | 44 | 0.00038 | 1Score > 37 indicates identity Score > 22 indicates homology |
U | R.FLVLYGIAAPDSQR.I | |
| 2402 |
775.4343 | 1548.8540 | 1548.8300 | 15.5 | 0 | 62 | 0.00012 | 1Score > 37 indicates identity Score > 35 indicates homology |
U | R.FLVLYGIAAPDSQR.I |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| FLGJ_PSEAE |
37 | 43130 | 64.1 | ||
| Peptidoglycan hydrolase flgJ OS=Pseudomonas aeruginosa GN=flgJ PE=3 SV=1 | |||||
| 34 samesets of FLGJ_PSEAE |
|||||
65| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YEAG_ECOLI | 79 | Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1 |
66| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL14_AZOSE | 78 | 50S ribosomal protein L14 OS=Azoarcus sp. (strain EbN1) GN=rplN PE=3 SV=1 |
67| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL29_PSE14 | 77 | 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1 |
68| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | OPRI_PSEAE | 77 | Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1 |
69| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ILVC_PSEE4 | 75 | Ketol-acid reductoisomerase OS=Pseudomonas entomophila (strain L48) GN=ilvC PE=3 SV=1 |
70| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH10_PSE14 | 75 | 10 kDa chaperonin OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=groS PE=3 SV=1 |
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