MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 61–70 (out of 179)

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-61

Accession Score Description
1 RL28_PSEE4 85 50S ribosomal protein L28 OS=Pseudomonas entomophila (strain L48) GN=rpmB PE=3 SV=1
Score Mass Matches Sequences emPAI
61.1 RL28_PSEE4 85 8988 3 (3) 2 (2) 0.62
50S ribosomal protein L28 OS=Pseudomonas entomophila (strain L48) GN=rpmB PE=3 SV=1
3 samesets of RL28_PSEE4
RL28_PSEP1 85 8974 3 (3) 2 (2) 0.62
50S ribosomal protein L28 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpmB PE=3 SV=1
RL28_PSEPG 85 8974 3 (3) 2 (2) 0.62
50S ribosomal protein L28 OS=Pseudomonas putida (strain GB-1) GN=rpmB PE=3 SV=1
RL28_PSEPK 85 8974 3 (3) 2 (2) 0.62
50S ribosomal protein L28 OS=Pseudomonas putida (strain KT2440) GN=rpmB PE=3 SV=1

-3 peptide matches (2 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1392 +1 535.8207 1069.6268 1069.6132 12.8 0 56 0.00076 +1Score > 38 indicates identity U R.GIDAVLVDIR.R
2148   474.9100 1421.7082 1421.7011 4.96 0 57 5.1e-005 +1Score > 41 indicates identity
Score > 26 indicates homology		 U K.GPVTGNNISHANNK.T

2 subsets and intersections (11 subset proteins in total)

Score Mass Subset of
RL28_VIBSL 58 9081 61.1
50S ribosomal protein L28 OS=Vibrio splendidus (strain LGP32) GN=rpmB PE=3 SV=1
RL28_AZOVD 57 9074 61.1
50S ribosomal protein L28 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpmB PE=3 SV=1
9 samesets of RL28_AZOVD
RL28_PSE14 57 8987
50S ribosomal protein L28 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmB PE=3 SV=1
RL28_PSEF5 57 9046
50S ribosomal protein L28 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpmB PE=3 SV=1
RL28_PSEFS 57 8902
50S ribosomal protein L28 OS=Pseudomonas fluorescens (strain SBW25) GN=rpmB PE=3 SV=1
RL28_PSEMY 57 9060
50S ribosomal protein L28 OS=Pseudomonas mendocina (strain ymp) GN=rpmB PE=3 SV=1
RL28_PSEPF 57 9003
50S ribosomal protein L28 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpmB PE=3 SV=1
RL28_PSEPW 57 9094
50S ribosomal protein L28 OS=Pseudomonas putida (strain W619) GN=rpmB PE=3 SV=1
RL28_PSESM 57 8987
50S ribosomal protein L28 OS=Pseudomonas syringae pv. tomato GN=rpmB PE=3 SV=1
RL28_PSEU2 57 8987
50S ribosomal protein L28 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpmB PE=3 SV=1
RL28_PSEU5 57 9060
50S ribosomal protein L28 OS=Pseudomonas stutzeri (strain A1501) GN=rpmB PE=3 SV=1

+62

Accession Score Description
1 CLPB_PSEPK 84 Chaperone protein clpB OS=Pseudomonas putida (strain KT2440) GN=clpB PE=3 SV=1

+63

Accession Score Description
1 ODO1_AZOVI 84 2-oxoglutarate dehydrogenase E1 component OS=Azotobacter vinelandii GN=sucA PE=3 SV=1

+64

Accession Score Description
1 KKA2_KLEPN 80 Aminoglycoside 3'-phosphotransferase OS=Klebsiella pneumoniae GN=neo PE=1 SV=1

+65

Accession Score Description
1 YEAG_ECOLI 79 Uncharacterized protein yeaG OS=Escherichia coli (strain K12) GN=yeaG PE=3 SV=1

+66

Accession Score Description
1 RL14_AZOSE 78 50S ribosomal protein L14 OS=Azoarcus sp. (strain EbN1) GN=rplN PE=3 SV=1

+67

Accession Score Description
1 RL29_PSE14 77 50S ribosomal protein L29 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpmC PE=3 SV=1

+68

Accession Score Description
1 OPRI_PSEAE 77 Major outer membrane lipoprotein OS=Pseudomonas aeruginosa GN=oprI PE=3 SV=1

+69

Accession Score Description
1 ILVC_PSEE4 75 Ketol-acid reductoisomerase OS=Pseudomonas entomophila (strain L48) GN=ilvC PE=3 SV=1

+70

Accession Score Description
1 CH10_PSE14 75 10 kDa chaperonin OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=groS PE=3 SV=1
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