MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 179)


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+51

Accession Score Description
1 ACP_PSEPK 108 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+52

Accession Score Description
1 RS9_PSEE4 102 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1

+53

Accession Score Description
1 LEU1_PSEP1 97 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

+54

Accession Score Description
1 IF2_CAMHC 96 Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1

+55

Accession Score Description
1 DHSB_ECOLI 95 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+56

Accession Score Description
1 ATPG_PSEPG 95 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

-57

Accession Score Description
1 RL7_PSEP1 90 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1
Score Mass Matches Sequences emPAI
57.1 RL7_PSEP1 90 12592 4 (2) 3 (1) 0.19
50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1
5 samesets of RL7_PSEP1
RL7_PSEPF 90 12606 4 (2) 3 (1) 0.19
50S ribosomal protein L7/L12 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplL PE=3 SV=1
RL7_PSEPG 90 12592 4 (2) 3 (1) 0.19
50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain GB-1) GN=rplL PE=3 SV=1
RL7_PSEPK 90 12592 4 (2) 3 (1) 0.19
50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain KT2440) GN=rplL PE=3 SV=2
RL7_PSEPU 90 12592 4 (2) 3 (1) 0.19
50S ribosomal protein L7/L12 OS=Pseudomonas putida GN=rplL PE=3 SV=2
RL7_PSEPW 90 12592 4 (2) 3 (1) 0.19
50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain W619) GN=rplL PE=3 SV=1

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
239   517.3022 516.2949 516.2907 8.10 0 19 1.6 +9Score > 48 indicates identity
Score > 34 indicates homology
E.AIGQK.T + Deamidated (NQ)
283   572.3787 571.3714 571.3693 3.65 0 27 0.43 +1Score > 40 indicates identity
Score > 35 indicates homology
K.VNVIK.A
2005 +1 678.3642 1354.7138 1354.7093 3.39 1 72 3.2e-005 +1Score > 40 indicates identity U K.VDGAPQVVAEGVSK.E

+58

Accession Score Description
1 CYOA_PSEPU 87 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+59

Accession Score Description
1 KPRS_PSEPK 86 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+60

Accession Score Description
1 ACCA_PSEPK 86 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1
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