MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 179)

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+51

Accession Score Description
1 ACP_PSEPK 108 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+52

Accession Score Description
1 RS9_PSEE4 102 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1

+53

Accession Score Description
1 LEU1_PSEP1 97 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

+54

Accession Score Description
1 IF2_CAMHC 96 Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1

+55

Accession Score Description
1 DHSB_ECOLI 95 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

-56

Accession Score Description
1 ATPG_PSEPG 95 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1
Score Mass Matches Sequences emPAI
56.1 ATPG_PSEPG 95 31653 9 (4) 7 (3) 0.24
ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1
2 samesets of ATPG_PSEPG
ATPG_PSEPK 95 31558 9 (4) 7 (3) 0.24
ATP synthase gamma chain OS=Pseudomonas putida (strain KT2440) GN=atpG PE=3 SV=1
ATPG_PSEP1 95 31558 9 (4) 7 (3) 0.24
ATP synthase gamma chain OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpG PE=3 SV=1

-9 peptide matches (8 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
223   503.3218 502.3145 502.3115 6.03 0 9 3.3 +5Score > 46 indicates identity
Score > 27 indicates homology		 K.VAVSK.M
1296   510.7926 1019.5706 1019.5651 5.43 1 19 0.31 +1Score > 39 indicates identity
Score > 26 indicates homology		 K.ELQLIYNK.A
1383   533.7822 1065.5498 1065.5455 4.11 0 47 0.00077 +1Score > 40 indicates identity
Score > 28 indicates homology		 U R.AGYIVVSSDR.G
1514 +1 566.2874 1130.5602 1130.5567 3.10 0 56 0.00012 +1Score > 42 indicates identity
Score > 29 indicates homology		 U K.NATDNAGDLIK.E
1519   566.7681 1131.5216 1131.5408 -16.9 0 10 0.59 +1Score > 40 indicates identity
Score > 20 indicates homology		 U K.NATDNAGDLIK.E + Deamidated (NQ)
2451   525.2449 1572.7129 1572.6997 8.36 0 23 0.31 +1Score > 39 indicates identity
Score > 30 indicates homology		 K.HHWDYLYEPDAK.E
2476   527.9445 1580.8117 1580.8059 3.65 1 36 0.0022 +1Score > 40 indicates identity
Score > 22 indicates homology		 U R.QVIGHLANANPEYR.H
4267   976.5312 2926.5718 2926.5362 12.2 1 12 1 +1Score > 33 indicates identity
Score > 24 indicates homology		 U K.FINTMTQKPTVEQLVPLVATPDQDLK.H + Deamidated (NQ)

3 subsets and intersections (12 subset proteins in total)

Score Mass Subset of
ATPG_PSEMY 65 31680 56.1
ATP synthase gamma chain OS=Pseudomonas mendocina (strain ymp) GN=atpG PE=3 SV=1
ATPG_PSEE4 58 31602 56.1
ATP synthase gamma chain OS=Pseudomonas entomophila (strain L48) GN=atpG PE=3 SV=1
1 sameset of ATPG_PSEE4
ATPG_PSEPW 58 31545
ATP synthase gamma chain OS=Pseudomonas putida (strain W619) GN=atpG PE=3 SV=1
ATPG_PSE14 36 31458 56.1
ATP synthase gamma chain OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpG PE=3 SV=1
8 samesets of ATPG_PSE14
ATPG_PSEAB 36 31704
ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=atpG PE=3 SV=1
ATPG_PSEAE 36 31704
ATP synthase gamma chain OS=Pseudomonas aeruginosa GN=atpG PE=3 SV=1
ATPG_PSEA7 36 31704
ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain PA7) GN=atpG PE=3 SV=1
ATPG_PSEA8 36 31704
ATP synthase gamma chain OS=Pseudomonas aeruginosa (strain LESB58) GN=atpG PE=3 SV=1
ATPG_PSEFS 36 31488
ATP synthase gamma chain OS=Pseudomonas fluorescens (strain SBW25) GN=atpG PE=3 SV=1
ATPG_PSEPF 36 31518
ATP synthase gamma chain OS=Pseudomonas fluorescens (strain Pf0-1) GN=atpG PE=3 SV=1
ATPG_PSEU2 36 31458
ATP synthase gamma chain OS=Pseudomonas syringae pv. syringae (strain B728a) GN=atpG PE=3 SV=1
ATPG_PSEF5 36 31532
ATP synthase gamma chain OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=atpG PE=3 SV=1

+57

Accession Score Description
1 RL7_PSEP1 90 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+58

Accession Score Description
1 CYOA_PSEPU 87 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+59

Accession Score Description
1 KPRS_PSEPK 86 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+60

Accession Score Description
1 ACCA_PSEPK 86 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1
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