MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 179)

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+51

Accession Score Description
1 ACP_PSEPK 108 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

+52

Accession Score Description
1 RS9_PSEE4 102 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1

+53

Accession Score Description
1 LEU1_PSEP1 97 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

-54

Accession Score Description
1 IF2_CAMHC 96 Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1
Score Mass Matches Sequences emPAI
54.1 IF2_CAMHC 96 100767 3 (1) 3 (1) 0.02
Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1
17 samesets of IF2_CAMHC
IF2_CAMLR 96 96725 3 (1) 3 (1) 0.02
Translation initiation factor IF-2 OS=Campylobacter lari (strain RM2100 / D67 / ATCC BAA-1060) GN=infB PE=3 SV=1
IF2_PSEE4 96 91973 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Pseudomonas entomophila (strain L48) GN=infB PE=3 SV=1
IF2_PSEP1 96 91620 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=infB PE=3 SV=1
IF2_PSEPG 96 91294 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Pseudomonas putida (strain GB-1) GN=infB PE=3 SV=1
IF2_PSEPK 96 91576 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Pseudomonas putida (strain KT2440) GN=infB PE=3 SV=1
IF2_PSEPW 96 91289 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Pseudomonas putida (strain W619) GN=infB PE=3 SV=1
IF2_SYNE7 96 110858 4 (1) 4 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus elongatus (strain PCC 7942) GN=infB PE=3 SV=1
IF2_SYNP6 96 110787 4 (1) 4 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) GN=infB PE=3 SV=1
IF2_MICAN 96 109614 3 (1) 3 (1) 0.02
Translation initiation factor IF-2 OS=Microcystis aeruginosa (strain NIES-843) GN=infB PE=3 SV=1
IF2_CYAP7 96 121749 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Cyanothece sp. (strain PCC 7424) GN=infB PE=3 SV=1
IF2_SYNP2 96 105884 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=infB PE=3 SV=1
IF2_THEEB 96 104469 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Thermosynechococcus elongatus (strain BP-1) GN=infB PE=3 SV=1
IF2_CYAP4 96 108559 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=infB PE=3 SV=1
IF2_SYNJA 96 111852 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. (strain JA-3-3Ab) GN=infB PE=3 SV=1
IF2_SYNJB 96 113485 2 (1) 2 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. GN=infB PE=3 SV=1
IF2_SYNPW 96 119022 1 (1) 1 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. (strain WH7803) GN=infB PE=3 SV=1
IF2_SYNS3 96 118793 1 (1) 1 (1) 0.02
Translation initiation factor IF-2 OS=Synechococcus sp. (strain CC9311) GN=infB PE=3 SV=1

-3 peptide matches (3 non-duplicate, 0 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
704   393.7281 785.4416 785.4395 2.70 1 18 6.8 +5Score > 43 indicates identity
Score > 39 indicates homology		 U E.IGQAEIR.Q
1192   490.7799 979.5452 979.5338 11.7 0 35 0.059 +1Score > 38 indicates identity
Score > 35 indicates homology		 K.TSLLDYIR.N
2185   720.4236 1438.8326 1438.8144 12.7 0 96 4.2e-008 +1Score > 35 indicates identity U R.GPVATIIVQNGTLR.V + Deamidated (NQ)

+55

Accession Score Description
1 DHSB_ECOLI 95 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+56

Accession Score Description
1 ATPG_PSEPG 95 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+57

Accession Score Description
1 RL7_PSEP1 90 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+58

Accession Score Description
1 CYOA_PSEPU 87 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+59

Accession Score Description
1 KPRS_PSEPK 86 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+60

Accession Score Description
1 ACCA_PSEPK 86 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1
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