MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 51–60 (out of 179)


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+51

Accession Score Description
1 ACP_PSEPK 108 Acyl carrier protein OS=Pseudomonas putida (strain KT2440) GN=acpP PE=3 SV=1

-52

Accession Score Description
1 RS9_PSEE4 102 30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
Score Mass Matches Sequences emPAI
52.1 RS9_PSEE4 102 14597 9 (6) 3 (3) 1.12
30S ribosomal protein S9 OS=Pseudomonas entomophila (strain L48) GN=rpsI PE=3 SV=1
3 samesets of RS9_PSEE4
RS9_PSEP1 102 14583 9 (6) 3 (3) 1.13
30S ribosomal protein S9 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsI PE=3 SV=1
RS9_PSEPG 102 14597 9 (6) 3 (3) 1.12
30S ribosomal protein S9 OS=Pseudomonas putida (strain GB-1) GN=rpsI PE=3 SV=1
RS9_PSEPK 102 14583 9 (6) 3 (3) 1.13
30S ribosomal protein S9 OS=Pseudomonas putida (strain KT2440) GN=rpsI PE=3 SV=1

-9 peptide matches (6 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1073 +2 470.7520 939.4894 939.4814 8.53 0 43 0.0091 +1Score > 39 indicates identity
Score > 35 indicates homology
U R.SLDVFFGR.E
1076   940.4989 939.4916 939.4814 10.9 0 25 0.13 +1Score > 39 indicates identity
Score > 29 indicates homology
U R.SLDVFFGR.E
1361 +1 527.7523 1053.4900 1053.4839 5.81 0 52 0.00083 +1Score > 40 indicates identity
Score > 34 indicates homology
U M.SATQNYGTGR.R
2649   553.3107 1656.9103 1656.9060 2.59 0 39 0.0023 +1Score > 37 indicates identity
Score > 26 indicates homology
U R.VFLRPGTGNISINNR.S
2650   829.4631 1656.9116 1656.9060 3.42 0 45 0.0025 +1Score > 37 indicates identity
Score > 31 indicates homology
U R.VFLRPGTGNISINNR.S
2652   553.6462 1657.9168 1657.8900 16.2 0 29 0.02 +1Score > 37 indicates identity
Score > 25 indicates homology
U R.VFLRPGTGNISINNR.S + Deamidated (NQ)

2 subsets and intersections (14 subset proteins in total)

Score Mass Subset of
RS9_PSE14 93 14593 52.1
30S ribosomal protein S9 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsI PE=3 SV=1
6 samesets of RS9_PSE14
RS9_PSEF5 93 14610
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsI PE=3 SV=1
RS9_PSEFS 93 14607
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsI PE=3 SV=1
RS9_PSEMY 93 14637
30S ribosomal protein S9 OS=Pseudomonas mendocina (strain ymp) GN=rpsI PE=3 SV=1
RS9_PSEPF 93 14597
30S ribosomal protein S9 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsI PE=3 SV=1
RS9_PSESM 93 14593
30S ribosomal protein S9 OS=Pseudomonas syringae pv. tomato GN=rpsI PE=3 SV=1
RS9_PSEU2 93 14593
30S ribosomal protein S9 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsI PE=3 SV=1
RS9_AZOVD 66 14723 52.1
30S ribosomal protein S9 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsI PE=3 SV=1
6 samesets of RS9_AZOVD
RS9_PSEA7 66 14616
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsI PE=3 SV=1
RS9_PSEA8 66 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsI PE=3 SV=1
RS9_PSEAB 66 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsI PE=3 SV=1
RS9_PSEAE 66 14606
30S ribosomal protein S9 OS=Pseudomonas aeruginosa GN=rpsI PE=3 SV=1
RS9_PSEPW 66 14581
30S ribosomal protein S9 OS=Pseudomonas putida (strain W619) GN=rpsI PE=3 SV=1
RS9_PSEU5 66 14604
30S ribosomal protein S9 OS=Pseudomonas stutzeri (strain A1501) GN=rpsI PE=3 SV=1

+53

Accession Score Description
1 LEU1_PSEP1 97 2-isopropylmalate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=leuA PE=3 SV=1

+54

Accession Score Description
1 IF2_CAMHC 96 Translation initiation factor IF-2 OS=Campylobacter hominis (strain ATCC BAA-381 / LMG 19568 / NCTC 13146 / CH001A) GN=infB PE=3 SV=1

+55

Accession Score Description
1 DHSB_ECOLI 95 Succinate dehydrogenase iron-sulfur subunit OS=Escherichia coli (strain K12) GN=sdhB PE=1 SV=1

+56

Accession Score Description
1 ATPG_PSEPG 95 ATP synthase gamma chain OS=Pseudomonas putida (strain GB-1) GN=atpG PE=3 SV=1

+57

Accession Score Description
1 RL7_PSEP1 90 50S ribosomal protein L7/L12 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplL PE=3 SV=1

+58

Accession Score Description
1 CYOA_PSEPU 87 Ubiquinol oxidase subunit 2 OS=Pseudomonas putida GN=cyoA PE=3 SV=1

+59

Accession Score Description
1 KPRS_PSEPK 86 Ribose-phosphate pyrophosphokinase OS=Pseudomonas putida (strain KT2440) GN=prs PE=3 SV=1

+60

Accession Score Description
1 ACCA_PSEPK 86 Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=accA PE=3 SV=1
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