MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU2_PSEPK | 1121 | Elongation factor Tu-B OS=Pseudomonas putida (strain KT2440) GN=tufB PE=3 SV=1 | |
| 6 | EFTU_CYAP7 | 40 | Elongation factor Tu OS=Cyanothece sp. (strain PCC 7424) GN=tuf PE=3 SV=1 | |
| 5 | EFTU_ANATD | 50 | Elongation factor Tu OS=Anaerocellum thermophilum (strain DSM 6725 / Z-1320) GN=tuf PE=3 SV=1 | |
| 3 | EFTU_PSEFS | 701 | Elongation factor Tu OS=Pseudomonas fluorescens (strain SBW25) GN=tuf PE=3 SV=1 | |
| 2 | EFTU1_PSEPK | 1002 | Elongation factor Tu-A OS=Pseudomonas putida (strain KT2440) GN=tufA PE=3 SV=1 | |
| 4 | EFTU_CARRP | 145 | Elongation factor Tu OS=Carsonella ruddii (strain PV) GN=tuf PE=3 SV=1 | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPB_PSEPG | 972 | ATP synthase subunit beta OS=Pseudomonas putida (strain GB-1) GN=atpD PE=3 SV=1 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPA_PSEPK | 749 | ATP synthase subunit alpha OS=Pseudomonas putida (strain KT2440) GN=atpA PE=3 SV=1 | |
| 2 | ATPA_RICAH | 179 | ATP synthase subunit alpha OS=Rickettsia akari (strain Hartford) GN=atpA PE=3 SV=1 | |
| 3 | ATPA_VEREI | 174 | ATP synthase subunit alpha OS=Verminephrobacter eiseniae (strain EF01-2) GN=atpA PE=3 SV=1 | |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | CH60_PSEPK | 584 | 60 kDa chaperonin OS=Pseudomonas putida (strain KT2440) GN=groL PE=3 SV=1 | |
| 3 | CH602_SORC5 | 80 | 60 kDa chaperonin 2 OS=Sorangium cellulosum (strain So ce56) GN=groL2 PE=3 SV=1 | |
| 2 | CH601_ECOK1 | 123 | 60 kDa chaperonin 1 OS=Escherichia coli O1:K1 / APEC GN=groL1 PE=3 SV=1 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DLDH2_PSEPU | 541 | Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 5.1 | DLDH2_PSEPU |
541 | 50093 | 38 (16) | 15 (7) | 0.50 | |
| Dihydrolipoamide dehydrogenase OS=Pseudomonas putida GN=lpdG PE=1 SV=4 | |||||||
38 peptide matches (22 non-duplicate, 16 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 210 |
2 |
487.3259 | 486.3186 | 486.3166 | 4.22 | 0 | 32 | 0.87 | 1Score > 46 indicates identity Score > 43 indicates homology |
K.AGIVK.N | |
| 220 |
501.3415 | 500.3342 | 500.3322 | 4.02 | 0 | 19 | 0.24 | 1Score > 42 indicates identity Score > 25 indicates homology |
K.LLAGK.K | ||
| 330 |
308.2142 | 614.4138 | 614.4115 | 3.77 | 1 | 19 | 0.96 | 1Score > 38 indicates identity Score > 31 indicates homology |
R.KAGIVK.N | ||
| 334 |
308.6874 | 615.3602 | 615.3592 | 1.77 | 0 | 23 | 0.8 | 1Score > 45 indicates identity Score > 35 indicates homology |
K.VIADAK.T | ||
| 337 |
616.3693 | 615.3620 | 615.3592 | 4.65 | 0 | 32 | 0.25 | 1Score > 45 indicates identity Score > 38 indicates homology |
K.VIADAK.T | ||
| 348 |
1 |
312.1741 | 622.3336 | 622.3326 | 1.64 | 0 | 22 | 0.91 | 1Score > 41 indicates identity Score > 34 indicates homology |
K.ITFDK.L | |
| 476 |
345.1967 | 688.3788 | 688.3755 | 4.81 | 1 | 24 | 0.87 | 1Score > 43 indicates identity Score > 36 indicates homology |
K.TEQALK.A | ||
| 477 |
1 |
689.3865 | 688.3792 | 688.3755 | 5.36 | 1 | 39 | 0.14 | 4Score > 43 indicates identity |
K.TEQALK.A | |
| 494 |
1 |
700.4371 | 699.4298 | 699.4279 | 2.75 | 0 | 54 | 0.0029 | 1Score > 41 indicates identity |
U | K.AAQLGLK.T |
| 497 |
350.7225 | 699.4304 | 699.4279 | 3.64 | 0 | 33 | 0.4 | 1Score > 41 indicates identity |
U | K.AAQLGLK.T | |
| 568 |
727.4853 | 726.4780 | 726.4752 | 3.88 | 0 | 23 | 0.77 | 1Score > 36 indicates identity Score > 35 indicates homology |
U | K.LIVAVGR.R | |
| 571 |
2 |
364.2469 | 726.4792 | 726.4752 | 5.57 | 0 | 30 | 0.13 | 1Score > 34 indicates identity |
U | K.LIVAVGR.R |
D:\Xcalibur\Data\Jennifer\PRT1270 Rita DDA\PRT1270_T-BRSC_2_20250714115216.rawScore > 42 indicates identity Score > 36 indicates homology |
|||||||||||
| 1013 |
2 |
460.2514 | 918.4882 | 918.4811 | 7.82 | 0 | 51 | 0.0016 | 1Score > 42 indicates identity Score > 36 indicates homology |
U | K.ALLDSSWK.Y |
| -15.2 | 0 | 21 | 2 | 2 | IAIDSVSSK | ||||||
| 4.17 | 0 | 21 | 2 | 3 | ALINNMVK + Deamidated (NQ); Oxidation (M) | ||||||
| 4.17 | 0 | 21 | 2 | 3 | ALINNVMK + Deamidated (NQ); Oxidation (M) | ||||||
| 4.15 | 0 | 21 | 2 | 3 | ALLDQMTK | ||||||
| -4.41 | 0 | 21 | 2 | 3 | ALLNQGFR + Deamidated (NQ) | ||||||
| 4.17 | 0 | 20 | 2.5 | 7 | ALNLSLCK + Deamidated (NQ) | ||||||
| 4.15 | 1 | 15 | 7.1 | 8 | ALDMKDVK | ||||||
| -8.08 | 1 | 12 | 13 | 9 | MKIGQAVR + Deamidated (NQ); Oxidation (M) | ||||||
| -15.2 | 0 | 11 | 17 | 10 | ALQVTTGTK + Deamidated (NQ) | ||||||
| 1490 |
1 |
1120.6448 | 1119.6375 | 1119.6288 | 7.78 | 0 | 82 | 1.7e-006 | 1Score > 37 indicates identity |
U | K.NLTGGVATLFK.A |
| 1492 |
1 |
560.8281 | 1119.6416 | 1119.6288 | 11.5 | 0 | 60 | 8.6e-005 | 1Score > 36 indicates identity Score > 32 indicates homology |
U | K.NLTGGVATLFK.A |
| 1596 |
584.8092 | 1167.6038 | 1167.5996 | 3.60 | 0 | 58 | 0.00011 | 1Score > 40 indicates identity Score > 31 indicates homology |
U | K.ANGVTSIQGHGK.L | |
| 1598 |
1 |
390.2094 | 1167.6064 | 1167.5996 | 5.77 | 0 | 24 | 0.067 | 1Score > 40 indicates identity Score > 25 indicates homology |
U | K.ANGVTSIQGHGK.L |
| 1954 |
444.6112 | 1330.8118 | 1330.7973 | 10.9 | 1 | 49 | 0.00035 | 1Score > 28 indicates identity Score > 27 indicates homology |
U | K.ITFDKLIVAVGR.R | |
| 2807 |
866.9979 | 1731.9812 | 1731.9560 | 14.6 | 0 | 41 | 0.00083 | 1Score > 34 indicates identity Score > 23 indicates homology |
U | K.FDVVVIGAGPGGYVAAIK.A | |
| 3027 |
2 |
934.0580 | 1866.1014 | 1866.0727 | 15.4 | 1 | 86 | 9.9e-008 | 1Score > 28 indicates identity |
U | R.LGVIGAGVIGLELGSVWAR.L |
| 3029 |
2 |
623.0414 | 1866.1024 | 1866.0727 | 15.9 | 1 | 103 | 1e-009 | 1Score > 28 indicates identity Score > 25 indicates homology |
U | R.LGVIGAGVIGLELGSVWAR.L |
| 4033 |
879.4454 | 2635.3144 | 2635.2629 | 19.5 | 0 | 4 | 1.1 | 2Score > 38 indicates identity Score > 17 indicates homology |
U | R.GYIFVDDYCATSVPGVYAIGDVVR.G | |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| DLDH2_PSEAE |
332 | 50362 | 5.1 | ||
| Dihydrolipoamide dehydrogenase OS=Pseudomonas aeruginosa GN=lpdG PE=3 SV=1 | |||||
| 1 sameset of DLDH2_PSEAE | |||||
| DLDH_PSEFL |
332 | 50348 | |||
| Dihydrolipoyl dehydrogenase OS=Pseudomonas fluorescens GN=lpd PE=1 SV=3 | |||||
| DLDH_AZOVI |
315 | 49707 | 5.1 | ||
| Dihydrolipoyl dehydrogenase OS=Azotobacter vinelandii PE=1 SV=1 | |||||
| DLDH2_ARATH |
55 | 54237 | 5.1 | ||
| Dihydrolipoyl dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana GN=LPD2 PE=1 SV=1 | |||||
| 3 samesets of DLDH2_ARATH | |||||
| DLDH_SOLTU |
55 | 3910 | |||
| Dihydrolipoyl dehydrogenase (Fragment) OS=Solanum tuberosum PE=1 SV=1 | |||||
| DLDH_TRYBB |
55 | 50815 | |||
| Dihydrolipoyl dehydrogenase OS=Trypanosoma brucei brucei PE=3 SV=1 | |||||
| DLDH2_BACSU |
55 | 50632 | |||
| Dihydrolipoyl dehydrogenase OS=Bacillus subtilis GN=bfmBC PE=3 SV=1 | |||||
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL1_PSEE4 | 419 | 50S ribosomal protein L1 OS=Pseudomonas entomophila (strain L48) GN=rplA PE=3 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFG1_PSEPK | 337 | Elongation factor G 1 OS=Pseudomonas putida (strain KT2440) GN=fusA PE=3 SV=1 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS5_PSEE4 | 331 | 30S ribosomal protein S5 OS=Pseudomonas entomophila (strain L48) GN=rpsE PE=3 SV=1 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL4_PSE14 | 327 | 50S ribosomal protein L4 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplD PE=3 SV=1 | |
| 2 | RL4_PSEE4 | 318 | 50S ribosomal protein L4 OS=Pseudomonas entomophila (strain L48) GN=rplD PE=3 SV=1 | |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL18_PSEP1 | 310 | 50S ribosomal protein L18 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplR PE=3 SV=1 |
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