MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 179)

Page: Previous 1 2 3 4 5 6 7 8 9 10 … 18 Next 

+41

Accession Score Description
1 ATPD_PSEPG 126 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1

+42

Accession Score Description
1 GUAA_CELJU 126 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+43

Accession Score Description
1 RISB_PSEPG 125 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

+44

Accession Score Description
1 DNAK_PSEPK 123 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1

+45

Accession Score Description
1 SYA_PSEPG 122 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2

+46

Accession Score Description
1 ODP2_PSEAE 122 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

-47

Accession Score Description
Family member distances as a dendrogram 1 RL20_PSEE4 118 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
2 RL20_POLNS 44 50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1
Cut threshold

Score Mass Matches Sequences emPAI
RL20_PSEE4 118 13272 13 (5) 9 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
4 samesets of RL20_PSEE4
RL20_PSEP1 118 13272 13 (5) 9 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplT PE=3 SV=1
RL20_PSEPG 118 13272 13 (5) 9 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas putida (strain GB-1) GN=rplT PE=3 SV=1
RL20_PSEPK 118 13272 13 (5) 9 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas putida (strain KT2440) GN=rplT PE=3 SV=1
RL20_PSEPW 118 13272 13 (5) 9 (4) 0.93
50S ribosomal protein L20 OS=Pseudomonas putida (strain W619) GN=rplT PE=3 SV=1
RL20_POLNS 44 13738 4 (2) 3 (1) 0.17
50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1
1 sameset of RL20_POLNS
RL20_POLSQ 44 13742 4 (2) 3 (1) 0.17
50S ribosomal protein L20 OS=Polynucleobacter sp. (strain QLW-P1DMWA-1) GN=rplT PE=3 SV=1

-14 peptide matches (10 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U 1 2 Peptide
266   558.3630 557.3557 557.3537 3.66 0 21 2.1 +8Score > 40 indicates identity
Score > 37 indicates homology		 X K.QAVIK.A
326 +1 307.7166 613.4186 613.4163 3.87 0 37 0.049 +1Score > 36 indicates identity U X R.LIAGLK.K
472   343.6675 685.3204 685.3184 3.05 0 4 1.5 +3Score > 37 indicates identity
Score > 18 indicates homology		 X K.GYYGAR.S
579   365.2257 728.4368 728.4333 4.84 0 19 1.1 +2Score > 41 indicates identity
Score > 32 indicates homology		 X X R.ALWIAR.I
600   371.7642 741.5138 741.5112 3.53 1 10 0.73 +3Score > 31 indicates identity
Score > 21 indicates homology		 U X R.LIAGLKK.A
783   414.7050 827.3954 827.3926 3.47 0 33 0.071 +1Score > 38 indicates identity
Score > 34 indicates homology		 X X AGQYAYR.D
950   449.2264 896.4382 896.4352 3.42 0 42 0.00075 +1Score > 40 indicates identity
Score > 23 indicates homology		 U X R.TNGLSYSR.L
1016   460.2688 918.5230 918.5287 -6.14 0 40 0.04 +1Score > 39 indicates identity U X K.AAFAALVTR.I
1395 +2 536.3100 1070.6054 1070.5972 7.75 1 70 4.8e-005 +1Score > 40 indicates identity U X K.VLADLAVNEK.A

7 subsets and intersections (50 subset proteins in total)

Score Mass Subset of
RL20_PSEF5 102 13287 47.1
50S ribosomal protein L20 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplT PE=3 SV=1
3 samesets of RL20_PSEF5
RL20_PSEFL 102 13287
50S ribosomal protein L20 OS=Pseudomonas fluorescens GN=rplT PE=3 SV=1
RL20_PSEMY 102 13241
50S ribosomal protein L20 OS=Pseudomonas mendocina (strain ymp) GN=rplT PE=3 SV=1
RL20_PSEU5 102 13284
50S ribosomal protein L20 OS=Pseudomonas stutzeri (strain A1501) GN=rplT PE=3 SV=1
RL20_PSE14 101 13306 47.1
50S ribosomal protein L20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplT PE=3 SV=1
4 samesets of RL20_PSE14
RL20_PSEPF 101 13306
50S ribosomal protein L20 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplT PE=3 SV=1
RL20_PSESM 101 13306
50S ribosomal protein L20 OS=Pseudomonas syringae pv. tomato GN=rplT PE=3 SV=1
RL20_PSESY 101 13306
50S ribosomal protein L20 OS=Pseudomonas syringae pv. syringae GN=rplT PE=3 SV=1
RL20_PSEU2 101 13306
50S ribosomal protein L20 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplT PE=3 SV=1
RL20_PSEA7 95 13357 47.1
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain PA7) GN=rplT PE=3 SV=1
3 samesets of RL20_PSEA7
RL20_PSEA8 95 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplT PE=3 SV=1
RL20_PSEAB 95 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplT PE=3 SV=1
RL20_PSEAE 95 13357
50S ribosomal protein L20 OS=Pseudomonas aeruginosa GN=rplT PE=3 SV=1
RL20_AZOVD 42 13385 47.1
50S ribosomal protein L20 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rplT PE=3 SV=1
1 sameset of RL20_AZOVD
RL20_AZOVI 42 13429
50S ribosomal protein L20 OS=Azotobacter vinelandii GN=rplT PE=3 SV=1
RL20_ERWT9 42 13490 47.1
50S ribosomal protein L20 OS=Erwinia tasmaniensis (strain DSM 17950 / Et1/99) GN=rplT PE=3 SV=1
RL20_PROMH 37 13449 47.1
50S ribosomal protein L20 OS=Proteus mirabilis (strain HI4320) GN=rplT PE=3 SV=1
ARLY_LACRD 37 52058 47.1
Argininosuccinate lyase OS=Lactobacillus reuteri (strain DSM 20016) GN=argH PE=3 SV=1
+32 samesets of ARLY_LACRD

+48

Accession Score Description
1 RL19_PSEE4 115 50S ribosomal protein L19 OS=Pseudomonas entomophila (strain L48) GN=rplS PE=3 SV=1

+49

Accession Score Description
1 RS20_PSE14 110 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+50

Accession Score Description
1 RL21_PSEE4 109 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1
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