MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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41| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPD_PSEPG | 126 | ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1 |
42| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | GUAA_CELJU | 126 | GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1 |
43| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RISB_PSEPG | 125 | 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1 |
44| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAK_PSEPK | 123 | Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1 |
45| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SYA_PSEPG | 122 | Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2 |
46| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODP2_PSEAE | 122 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 46.1 | ODP2_PSEAE |
122 | 56788 | 9 (5) | 6 (3) | 0.13 | |
| Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2 | |||||||
9 peptide matches (6 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 196 |
1 |
473.3099 | 472.3026 | 472.3009 | 3.58 | 0 | 21 | 1.5 | 3Score > 40 indicates identity Score > 36 indicates homology |
K.AGVVK.S | |
| 339 |
309.1858 | 616.3570 | 616.3544 | 4.29 | 1 | 20 | 0.99 | 1Score > 44 indicates identity Score > 33 indicates homology |
M.SELIR.V | ||
| 816 |
421.7468 | 841.4790 | 841.4770 | 2.46 | 0 | 53 | 0.0023 | 1Score > 39 indicates identity |
U | R.VINGAAAAR.F | |
| 1261 |
2 |
505.3604 | 1008.7062 | 1008.6947 | 11.5 | 0 | 48 | 1.6e-005 | 1Score > 13 indicates identity |
U | K.LTVLPILLK.A |
| 1491 |
560.8268 | 1119.6390 | 1119.6400 | -0.85 | 0 | 2 | 7.2 | 7Score > 37 indicates identity Score > 23 indicates homology |
U | E.QKPAAAAPAPAK.A | |
| 2223 |
731.3689 | 1460.7232 | 1460.7147 | 5.85 | 1 | 42 | 0.00078 | 1Score > 41 indicates identity Score > 24 indicates homology |
U | K.ELPDFNSSLAPSGK.A |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ODP2_AZOVI |
102 | 65119 | 46.1 | ||
| Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Azotobacter vinelandii PE=1 SV=3 | |||||
47| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL20_PSEE4 | 118 | 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1 | |
| 2 | RL20_POLNS | 44 | 50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1 | |
48| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL19_PSEE4 | 115 | 50S ribosomal protein L19 OS=Pseudomonas entomophila (strain L48) GN=rplS PE=3 SV=1 |
49| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS20_PSE14 | 110 | 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1 |
50| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL21_PSEE4 | 109 | 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1 |
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