MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 41–50 (out of 179)

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+41

Accession Score Description
1 ATPD_PSEPG 126 ATP synthase subunit delta OS=Pseudomonas putida (strain GB-1) GN=atpH PE=3 SV=1

+42

Accession Score Description
1 GUAA_CELJU 126 GMP synthase [glutamine-hydrolyzing] OS=Cellvibrio japonicus (strain Ueda107) GN=guaA PE=3 SV=1

+43

Accession Score Description
1 RISB_PSEPG 125 6,7-dimethyl-8-ribityllumazine synthase OS=Pseudomonas putida (strain GB-1) GN=ribH PE=3 SV=1

-44

Accession Score Description
1 DNAK_PSEPK 123 Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1
Score Mass Matches Sequences emPAI
44.1 DNAK_PSEPK 123 68873 6 (3) 5 (3) 0.10
Chaperone protein dnaK OS=Pseudomonas putida (strain KT2440) GN=dnaK PE=2 SV=1
1 sameset of DNAK_PSEPK
DNAK_PSEP1 123 65566 6 (3) 5 (3) 0.11
Chaperone protein dnaK OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=dnaK PE=2 SV=1

-6 peptide matches (5 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1661   399.8719 1196.5939 1196.5898 3.40 0 19 1.6 +3Score > 40 indicates identity
Score > 34 indicates homology		 R.NQGDALVHSTR.K
1856   643.3656 1284.7166 1284.7038 10.0 0 58 0.00072 +1Score > 39 indicates identity U K.IDDVILVGGQTR.M
2501   532.9811 1595.9215 1595.9022 12.1 0 1 1.5 +3Score > 34 indicates identity
Score > 15 indicates homology		 K.DVLLLDVSPLTLGIE.T
3426 +1 1067.5731 2133.1316 2133.0954 17.0 1 31 0.0043 +1Score > 37 indicates identity
Score > 19 indicates homology		 U R.TTPSIVAYANDGEILVGQSAK.R
3924   835.0862 2502.2368 2502.1987 15.2 1 99 5e-008 +1Score > 38 indicates identity U K.SQVFSTADDNQSAVTIHVLQGER.K + Deamidated (NQ)

4 subsets and intersections (27 subset proteins in total)

Score Mass Subset of
DNAK_PSEMY 118 68817 44.1
Chaperone protein dnaK OS=Pseudomonas mendocina (strain ymp) GN=dnaK PE=2 SV=1
1 sameset of DNAK_PSEMY
DNAK_PSEU5 118 68693
Chaperone protein dnaK OS=Pseudomonas stutzeri (strain A1501) GN=dnaK PE=2 SV=1
DNAK_TOLAT 99 70189 44.1
Chaperone protein dnaK OS=Tolumonas auensis (strain DSM 9187 / TA4) GN=dnaK PE=2 SV=1
DNAK_PSEPG 60 68670 44.1
Chaperone protein dnaK OS=Pseudomonas putida (strain GB-1) GN=dnaK PE=2 SV=1
1 sameset of DNAK_PSEPG
DNAK_PSEPW 60 68873
Chaperone protein dnaK OS=Pseudomonas putida (strain W619) GN=dnaK PE=2 SV=1
DNAK_ACTSZ 58 68436 44.1
Chaperone protein dnaK OS=Actinobacillus succinogenes (strain ATCC 55618 / 130Z) GN=dnaK PE=2 SV=1
+21 samesets of DNAK_ACTSZ

+45

Accession Score Description
1 SYA_PSEPG 122 Alanyl-tRNA synthetase OS=Pseudomonas putida (strain GB-1) GN=alaS PE=3 SV=2

+46

Accession Score Description
1 ODP2_PSEAE 122 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OS=Pseudomonas aeruginosa GN=aceF PE=3 SV=2

+47

Accession Score Description
Family member distances as a dendrogram 1 RL20_PSEE4 118 50S ribosomal protein L20 OS=Pseudomonas entomophila (strain L48) GN=rplT PE=3 SV=1
2 RL20_POLNS 44 50S ribosomal protein L20 OS=Polynucleobacter necessarius (strain STIR1) GN=rplT PE=3 SV=1

+48

Accession Score Description
1 RL19_PSEE4 115 50S ribosomal protein L19 OS=Pseudomonas entomophila (strain L48) GN=rplS PE=3 SV=1

+49

Accession Score Description
1 RS20_PSE14 110 30S ribosomal protein S20 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsT PE=3 SV=1

+50

Accession Score Description
1 RL21_PSEE4 109 50S ribosomal protein L21 OS=Pseudomonas entomophila (strain L48) GN=rplU PE=3 SV=1
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