MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 179)


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+31

Accession Score Description
1 ATPF_PSEPK 165 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+32

Accession Score Description
1 RL22_PSE14 163 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1

+33

Accession Score Description
1 RS11_AZOVD 161 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1

+34

Accession Score Description
1 ETFB_PSEAE 154 Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

+35

Accession Score Description
1 RL6_PSEP1 153 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1

+36

Accession Score Description
1 RL25_PSEPK 151 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+37

Accession Score Description
1 ODB2_PSEPU 150 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+38

Accession Score Description
1 NUOG_PSEPK 150 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1

-39

Accession Score Description
1 RS2_PSEP1 138 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1
Score Mass Matches Sequences emPAI
39.1 RS2_PSEP1 138 27063 19 (7) 9 (4) 0.39
30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1
1 sameset of RS2_PSEP1
RS2_PSEPK 138 27063 19 (7) 9 (4) 0.39
30S ribosomal protein S2 OS=Pseudomonas putida (strain KT2440) GN=rpsB PE=3 SV=1

+19 peptide matches (10 non-duplicate, 9 duplicate)

8 subsets and intersections (393 subset proteins in total)

Score Mass Subset of
RS2_PSEPW 131 27095 39.1
30S ribosomal protein S2 OS=Pseudomonas putida (strain W619) GN=rpsB PE=3 SV=1
RS2_PSE14 106 27257 39.1
30S ribosomal protein S2 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsB PE=3 SV=1
6 samesets of RS2_PSE14
RS2_PSEF5 106 27057
30S ribosomal protein S2 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsB PE=3 SV=1
RS2_PSEMY 106 27219
30S ribosomal protein S2 OS=Pseudomonas mendocina (strain ymp) GN=rpsB PE=3 SV=1
RS2_PSEFS 106 27135
30S ribosomal protein S2 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsB PE=3 SV=1
RS2_PSEPF 106 27102
30S ribosomal protein S2 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsB PE=3 SV=1
RS2_PSESM 106 27241
30S ribosomal protein S2 OS=Pseudomonas syringae pv. tomato GN=rpsB PE=3 SV=1
RS2_PSEU5 106 27306
30S ribosomal protein S2 OS=Pseudomonas stutzeri (strain A1501) GN=rpsB PE=3 SV=1
RS2_AZOVD 100 27383 39.1
30S ribosomal protein S2 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsB PE=3 SV=1
1 sameset of RS2_AZOVD
RS2_PSEU2 100 27302
30S ribosomal protein S2 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsB PE=3 SV=1
RS2_PSEPG 99 27032 39.1
30S ribosomal protein S2 OS=Pseudomonas putida (strain GB-1) GN=rpsB PE=3 SV=1
RS2_CELJU 55 27098 39.1
30S ribosomal protein S2 OS=Cellvibrio japonicus (strain Ueda107) GN=rpsB PE=3 SV=1
RS2_ALCBS 45 28216 39.1
30S ribosomal protein S2 OS=Alcanivorax borkumensis (strain SK2 / ATCC 700651 / DSM 11573) GN=rpsB PE=3 SV=1
+75 samesets of RS2_ALCBS
COAX_PETMO 39 28537 39.1
Type III pantothenate kinase OS=Petrotoga mobilis (strain DSM 10674 / SJ95) GN=coaX PE=3 SV=1
+76 samesets of COAX_PETMO
RR2_CHLAT 35 25793 39.1
30S ribosomal protein S2, chloroplastic OS=Chlorokybus atmophyticus GN=rps2 PE=3 SV=2
+227 samesets of RR2_CHLAT

+40

Accession Score Description
1 SAHH_PSEPF 131 Adenosylhomocysteinase OS=Pseudomonas fluorescens (strain Pf0-1) GN=ahcY PE=3 SV=1
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