MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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31| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPF_PSEPK | 165 | ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1 |
32| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL22_PSE14 | 163 | 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1 |
33| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS11_AZOVD | 161 | 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1 |
34| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ETFB_PSEAE | 154 | Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1 |
35| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL6_PSEP1 | 153 | 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1 |
36| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL25_PSEPK | 151 | 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 |
37| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODB2_PSEPU | 150 | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 37.1 | ODB2_PSEPU |
150 | 45158 | 8 (5) | 7 (4) | 0.22 | |
| Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1 | |||||||
8 peptide matches (7 non-duplicate, 1 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 598 |
371.2546 | 740.4946 | 740.4908 | 5.14 | 0 | 23 | 0.44 | 1Score > 32 indicates identity |
U | R.ALVVALR.D | |
| 1133 |
479.2743 | 956.5340 | 956.5291 | 5.21 | 1 | 32 | 0.16 | 1Score > 41 indicates identity Score > 37 indicates homology |
U | R.ALDAGIELR.Y | |
| 1239 |
334.1719 | 999.4939 | 999.4886 | 5.24 | 0 | 13 | 0.88 | 1Score > 40 indicates identity Score > 25 indicates homology |
U | R.YVHGSGPAGR.I | |
| 1400 |
1 |
536.3585 | 1070.7024 | 1070.6852 | 16.1 | 0 | 45 | 0.00034 | 1Score > 23 indicates identity |
U | K.LTLLPFLVR.A |
| 1798 |
419.6120 | 1255.8142 | 1255.8016 | 10.00 | 1 | 30 | 0.0053 | 1Score > 20 indicates identity |
U | R.GKLTLLPFLVR.A | |
| 1960 |
445.9193 | 1334.7361 | 1334.7306 | 4.07 | 0 | 41 | 0.00065 | 1Score > 38 indicates identity Score > 22 indicates homology |
U | R.QPGDKPLASPAVR.K | |
| 3379 |
1056.0167 | 2110.0188 | 2109.9855 | 15.8 | 1 | 85 | 4.2e-008 | 1Score > 39 indicates identity Score > 24 indicates homology |
U | R.DFPQINATYDDEAQIITR.H + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ODB2_PSEAE |
70 | 45746 | 37.1 | ||
| Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas aeruginosa GN=bkdB PE=1 SV=1 | |||||
38| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NUOG_PSEPK | 150 | NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1 |
39| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEP1 | 138 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1 |
40| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SAHH_PSEPF | 131 | Adenosylhomocysteinase OS=Pseudomonas fluorescens (strain Pf0-1) GN=ahcY PE=3 SV=1 |
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