MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 179)

Page: Previous 1 2 3 4 5 6 7 8 9 … 18 Next 

+31

Accession Score Description
1 ATPF_PSEPK 165 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+32

Accession Score Description
1 RL22_PSE14 163 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1

+33

Accession Score Description
1 RS11_AZOVD 161 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1

+34

Accession Score Description
1 ETFB_PSEAE 154 Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

-35

Accession Score Description
1 RL6_PSEP1 153 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1
Score Mass Matches Sequences emPAI
35.1 RL6_PSEP1 153 19131 10 (5) 6 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1
6 samesets of RL6_PSEP1
RL6_PSEPG 153 19131 10 (5) 6 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain GB-1) GN=rplF PE=3 SV=1
RL6_PSEPK 153 19131 10 (5) 6 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain KT2440) GN=rplF PE=3 SV=1
RL6_PSEF5 153 19170 9 (5) 5 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rplF PE=3 SV=1
RL6_PSEFS 153 19185 9 (5) 5 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain SBW25) GN=rplF PE=3 SV=1
RL6_PSEPF 153 19184 9 (5) 5 (3) 0.41
50S ribosomal protein L6 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rplF PE=3 SV=1
RL6_PSEPW 153 19115 9 (5) 5 (3) 0.42
50S ribosomal protein L6 OS=Pseudomonas putida (strain W619) GN=rplF PE=3 SV=1

-10 peptide matches (7 non-duplicate, 3 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
785   414.7475 827.4804 827.4753 6.26 1 20 1.3 +2Score > 40 indicates identity
Score > 33 indicates homology		 K.LPSGVEVK.F
844   426.2184 850.4222 850.4185 4.44 1 11 0.59 +1Score > 42 indicates identity
Score > 21 indicates homology		 R.YADEVVR.R
1187 +1 489.2764 976.5382 976.5342 4.17 0 58 0.0011 +1Score > 41 indicates identity U K.FAGQQLSVK.G
1460   368.8992 1103.6758 1103.6703 4.98 1 23 0.26 +1Score > 30 indicates identity
Score > 30 indicates homology		 U R.KLQLVGVGYK.A
1461   552.8461 1103.6776 1103.6703 6.68 1 35 0.02 +1Score > 30 indicates identity U R.KLQLVGVGYK.A
1557 +1 383.5309 1147.5709 1147.5662 4.08 0 18 0.26 +1Score > 42 indicates identity
Score > 25 indicates homology		 U R.DFRPPEPYK.G
1637 +1 592.3473 1182.6800 1182.6721 6.76 1 83 1.7e-006 +1Score > 38 indicates identity U K.QLVGQVAAEIR.D

4 subsets and intersections (21 subset proteins in total)

Score Mass Subset of
RL6_PSE14 128 19506 35.1
50S ribosomal protein L6 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplF PE=3 SV=1
8 samesets of RL6_PSE14
RL6_PSESM 128 19266
50S ribosomal protein L6 OS=Pseudomonas syringae pv. tomato GN=rplF PE=3 SV=1
RL6_PSEU2 128 19236
50S ribosomal protein L6 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rplF PE=3 SV=1
RL6_PSEA7 128 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain PA7) GN=rplF PE=3 SV=1
RL6_PSEA8 128 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain LESB58) GN=rplF PE=3 SV=1
RL6_PSEAB 128 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rplF PE=3 SV=1
RL6_PSEAE 128 19087
50S ribosomal protein L6 OS=Pseudomonas aeruginosa GN=rplF PE=3 SV=1
RL6_PSEMY 128 19130
50S ribosomal protein L6 OS=Pseudomonas mendocina (strain ymp) GN=rplF PE=3 SV=1
RL6_PSEU5 128 19167
50S ribosomal protein L6 OS=Pseudomonas stutzeri (strain A1501) GN=rplF PE=3 SV=1
RL6_CHRVO 125 18841 35.1
50S ribosomal protein L6 OS=Chromobacterium violaceum GN=rplF PE=3 SV=1
3 samesets of RL6_CHRVO
RL6_SHELP 125 18777
50S ribosomal protein L6 OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) GN=rplF PE=3 SV=1
RL6_SHESH 125 18751
50S ribosomal protein L6 OS=Shewanella sediminis (strain HAW-EB3) GN=rplF PE=3 SV=1
RL6_SHEWM 125 18852
50S ribosomal protein L6 OS=Shewanella woodyi (strain ATCC 51908 / MS32) GN=rplF PE=3 SV=1
RL6_PSEE4 65 19015 35.1
50S ribosomal protein L6 OS=Pseudomonas entomophila (strain L48) GN=rplF PE=3 SV=1
RL6_ACIB3 35 19085 35.1
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AB307-0294) GN=rplF PE=3 SV=1
6 samesets of RL6_ACIB3
RL6_ACIB5 35 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AB0057) GN=rplF PE=3 SV=1
RL6_ACIBS 35 19113
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain SDF) GN=rplF PE=3 SV=1
RL6_ACIBT 35 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain ATCC 17978 / NCDC KC 755) GN=rplF PE=3 SV=2
RL6_ACIBY 35 19085
50S ribosomal protein L6 OS=Acinetobacter baumannii (strain AYE) GN=rplF PE=3 SV=1
RL6_AERHH 35 18568
50S ribosomal protein L6 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=rplF PE=3 SV=1
RL6_AERS4 35 18556
50S ribosomal protein L6 OS=Aeromonas salmonicida (strain A449) GN=rplF PE=3 SV=1

+36

Accession Score Description
1 RL25_PSEPK 151 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+37

Accession Score Description
1 ODB2_PSEPU 150 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+38

Accession Score Description
1 NUOG_PSEPK 150 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1

+39

Accession Score Description
1 RS2_PSEP1 138 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+40

Accession Score Description
1 SAHH_PSEPF 131 Adenosylhomocysteinase OS=Pseudomonas fluorescens (strain Pf0-1) GN=ahcY PE=3 SV=1
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