MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 179)

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+31

Accession Score Description
1 ATPF_PSEPK 165 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+32

Accession Score Description
1 RL22_PSE14 163 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1

+33

Accession Score Description
1 RS11_AZOVD 161 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1

-34

Accession Score Description
1 ETFB_PSEAE 154 Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1
Score Mass Matches Sequences emPAI
34.1 ETFB_PSEAE 154 26417 9 (5) 3 (1) 0.29
Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

-9 peptide matches (5 non-duplicate, 4 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
534   357.2021 712.3896 712.3868 4.05 1 18 0.33 +1Score > 38 indicates identity
Score > 26 indicates homology		 U K.VEAPAAR.S
931 +1 444.2527 886.4908 886.4872 4.10 0 27 1.4 +2Score > 42 indicates identity
Score > 41 indicates homology		 U R.TALALGADR.A
3729 +3 770.4375 2308.2907 2308.2638 11.6 1 39 0.0012 +1Score > 32 indicates identity
Score > 22 indicates homology		 U R.EIDGGLQTVALNLPAIVTTDLR.L
3730   1155.1528 2308.2910 2308.2638 11.8 1 59 3.5e-005 +1Score > 32 indicates identity
Score > 27 indicates homology		 U R.EIDGGLQTVALNLPAIVTTDLR.L
3733   1155.6514 2309.2882 2309.2478 17.5 1 77 2.1e-007 +1Score > 32 indicates identity
Score > 23 indicates homology		 U R.EIDGGLQTVALNLPAIVTTDLR.L + Deamidated (NQ)

+35

Accession Score Description
1 RL6_PSEP1 153 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1

+36

Accession Score Description
1 RL25_PSEPK 151 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+37

Accession Score Description
1 ODB2_PSEPU 150 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+38

Accession Score Description
1 NUOG_PSEPK 150 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1

+39

Accession Score Description
1 RS2_PSEP1 138 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+40

Accession Score Description
1 SAHH_PSEPF 131 Adenosylhomocysteinase OS=Pseudomonas fluorescens (strain Pf0-1) GN=ahcY PE=3 SV=1
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