MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita2 sp
MS data file : PRT1270_T-BRSC_2_20250714115216.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:39:15 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,778

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 31–40 (out of 179)

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+31

Accession Score Description
1 ATPF_PSEPK 165 ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1

+32

Accession Score Description
1 RL22_PSE14 163 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1

-33

Accession Score Description
1 RS11_AZOVD 161 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1
Score Mass Matches Sequences emPAI
33.1 RS11_AZOVD 161 13735 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1
17 samesets of RS11_AZOVD
RS11_PSE14 161 13721 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rpsK PE=3 SV=1
RS11_PSEA7 161 13735 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas aeruginosa (strain PA7) GN=rpsK PE=3 SV=1
RS11_PSEA8 161 13735 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas aeruginosa (strain LESB58) GN=rpsK PE=3 SV=1
RS11_PSEAB 161 13735 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=rpsK PE=3 SV=1
RS11_PSEAE 161 13735 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas aeruginosa GN=rpsK PE=3 SV=1
RS11_PSEE4 161 13765 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas entomophila (strain L48) GN=rpsK PE=3 SV=1
RS11_PSEF5 161 13735 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rpsK PE=3 SV=1
RS11_PSEFS 161 13721 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas fluorescens (strain SBW25) GN=rpsK PE=3 SV=1
RS11_PSEMY 161 13753 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas mendocina (strain ymp) GN=rpsK PE=3 SV=1
RS11_PSEP1 161 13765 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsK PE=3 SV=1
RS11_PSEPF 161 13721 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas fluorescens (strain Pf0-1) GN=rpsK PE=3 SV=1
RS11_PSEPG 161 13779 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas putida (strain GB-1) GN=rpsK PE=3 SV=1
RS11_PSEPK 161 13765 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas putida (strain KT2440) GN=rpsK PE=3 SV=1
RS11_PSEPW 161 13765 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas putida (strain W619) GN=rpsK PE=3 SV=1
RS11_PSESM 161 13721 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas syringae pv. tomato GN=rpsK PE=3 SV=1
RS11_PSEU2 161 13721 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rpsK PE=3 SV=1
RS11_PSEU5 161 13749 4 (4) 3 (3) 0.61
30S ribosomal protein S11 OS=Pseudomonas stutzeri (strain A1501) GN=rpsK PE=3 SV=1

-4 peptide matches (3 non-duplicate, 1 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
1485   560.8043 1119.5940 1119.5924 1.48 1 53 0.0031 +1Score > 41 indicates identity U R.AGQAALEYGLK.N
2498 +1 798.3843 1594.7540 1594.7488 3.28 0 92 4.4e-007 +1Score > 40 indicates identity U R.QGNALSWATSGGSGFR.G
3919   832.4509 2494.3309 2494.2816 19.7 0 43 0.01 +1Score > 35 indicates identity U K.TVVDGIAHIHASFNNTIVTITDR.Q + Deamidated (NQ)

2 subsets and intersections (34 subset proteins in total)

Score Mass Subset of
RS11_AERHH 141 13883 33.1
30S ribosomal protein S11 OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=rpsK PE=3 SV=1
+31 samesets of RS11_AERHH
RS11_SACD2 43 13955 33.1
30S ribosomal protein S11 OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=rpsK PE=3 SV=1
1 sameset of RS11_SACD2
RS11_TERTT 43 13913
30S ribosomal protein S11 OS=Teredinibacter turnerae (strain ATCC 39867 / T7901) GN=rpsK PE=3 SV=1

+34

Accession Score Description
1 ETFB_PSEAE 154 Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1

+35

Accession Score Description
1 RL6_PSEP1 153 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1

+36

Accession Score Description
1 RL25_PSEPK 151 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2

+37

Accession Score Description
1 ODB2_PSEPU 150 Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1

+38

Accession Score Description
1 NUOG_PSEPK 150 NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1

+39

Accession Score Description
1 RS2_PSEP1 138 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1

+40

Accession Score Description
1 SAHH_PSEPF 131 Adenosylhomocysteinase OS=Pseudomonas fluorescens (strain Pf0-1) GN=ahcY PE=3 SV=1
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