MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Rita2 sp |
| MS data file | : | PRT1270_T-BRSC_2_20250714115216.mgf |
| Database | : | SwissProt 57.15 (515,203 sequences; 181,334,896 residues) |
| Timestamp | : | 12 Aug 2025 at 23:39:15 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,778 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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31| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATPF_PSEPK | 165 | ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 31.1 | ATPF_PSEPK |
165 | 16945 | 12 (9) | 6 (4) | 0.92 | |
| ATP synthase subunit b OS=Pseudomonas putida (strain KT2440) GN=atpF PE=3 SV=1 | |||||||
| 1 sameset of ATPF_PSEPK | |||||||
| ATPF_PSEE4 |
165 | 17016 | 11 (9) | 5 (4) | 0.92 | ||
| ATP synthase subunit b OS=Pseudomonas entomophila (strain L48) GN=atpF PE=3 SV=1 | |||||||
12 peptide matches (8 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 237 |
1 |
516.3044 | 515.2971 | 515.2955 | 3.18 | 1 | 18 | 1.5 | 2Score > 47 indicates identity Score > 32 indicates homology |
K.LAAEI.- | |
| 434 |
336.6938 | 671.3730 | 671.3715 | 2.37 | 0 | 34 | 0.13 | 1Score > 41 indicates identity Score > 38 indicates homology |
K.AGQQLR.E | ||
| 1170 |
1 |
486.7601 | 971.5056 | 971.5036 | 2.12 | 1 | 53 | 0.00051 | 1Score > 41 indicates identity Score > 33 indicates homology |
U | R.AAQLVDEAR.E |
| 1280 |
508.2637 | 1014.5128 | 1014.5094 | 3.40 | 0 | 50 | 0.006 | 1Score > 40 indicates identity |
U | K.IADGLDAANR.A | |
| 1602 |
2 |
585.8305 | 1169.6464 | 1169.6404 | 5.15 | 1 | 50 | 0.0048 | 1Score > 40 indicates identity |
U | R.AQVGALAVGGAEK.I |
| 2930 |
602.9993 | 1805.9761 | 1805.9635 | 6.94 | 1 | 40 | 0.00055 | 1Score > 37 indicates identity Score > 20 indicates homology |
U | K.ILGATIDQNAHAELVNK.L | |
| 2936 |
603.3323 | 1806.9751 | 1806.9475 | 15.2 | 1 | 59 | 5e-005 | 1Score > 37 indicates identity Score > 29 indicates homology |
U | K.ILGATIDQNAHAELVNK.L + Deamidated (NQ) | |
| 2937 |
603.3326 | 1806.9760 | 1806.9475 | 15.7 | 1 | 31 | 0.014 | 1Score > 37 indicates identity Score > 25 indicates homology |
U | K.ILGATIDQNAHAELVNK.L + Deamidated (NQ) |
| Score | Mass | Subset of | |||
|---|---|---|---|---|---|
| ATPF_PSEP1 |
135 | 16959 | 31.1 | ||
| ATP synthase subunit b OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=atpF PE=3 SV=1 | |||||
| 2 samesets of ATPF_PSEP1 | |||||
| ATPF_PSEPG |
135 | 16945 | |||
| ATP synthase subunit b OS=Pseudomonas putida (strain GB-1) GN=atpF PE=3 SV=1 | |||||
| ATPF_PSEPW |
135 | 16945 | |||
| ATP synthase subunit b OS=Pseudomonas putida (strain W619) GN=atpF PE=3 SV=1 | |||||
| ATPF_PSE14 |
80 | 16988 | 31.1 | ||
| ATP synthase subunit b OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=atpF PE=3 SV=1 | |||||
| 3 samesets of ATPF_PSE14 | |||||
| ATPF_PSEU2 |
80 | 16988 | |||
| ATP synthase subunit b OS=Pseudomonas syringae pv. syringae (strain B728a) GN=atpF PE=3 SV=1 | |||||
| ATPF_PSEF5 |
80 | 16981 | |||
| ATP synthase subunit b OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=atpF PE=3 SV=1 | |||||
| ATPF_PSESM |
80 | 17018 | |||
| ATP synthase subunit b OS=Pseudomonas syringae pv. tomato GN=atpF PE=3 SV=1 | |||||
| ATPF_PSEA7 |
50 | 17003 | 31.1 | ||
| ATP synthase subunit b OS=Pseudomonas aeruginosa (strain PA7) GN=atpF PE=3 SV=1 | |||||
| 2 samesets of ATPF_PSEA7 | |||||
| ATPF_PSEAB |
50 | 17003 | |||
| ATP synthase subunit b OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=atpF PE=3 SV=1 | |||||
| ATPF_PSEAE |
50 | 17003 | |||
| ATP synthase subunit b OS=Pseudomonas aeruginosa GN=atpF PE=3 SV=1 | |||||
32| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL22_PSE14 | 163 | 50S ribosomal protein L22 OS=Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6) GN=rplV PE=3 SV=1 |
33| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS11_AZOVD | 161 | 30S ribosomal protein S11 OS=Azotobacter vinelandii (strain DJ / ATCC BAA-1303) GN=rpsK PE=3 SV=1 |
34| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ETFB_PSEAE | 154 | Electron transfer flavoprotein subunit beta OS=Pseudomonas aeruginosa GN=etfB PE=3 SV=1 |
35| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL6_PSEP1 | 153 | 50S ribosomal protein L6 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rplF PE=3 SV=1 |
36| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RL25_PSEPK | 151 | 50S ribosomal protein L25 OS=Pseudomonas putida (strain KT2440) GN=rplY PE=3 SV=2 |
37| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ODB2_PSEPU | 150 | Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex OS=Pseudomonas putida GN=bkdB PE=3 SV=1 |
38| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | NUOG_PSEPK | 150 | NADH-quinone oxidoreductase subunit G OS=Pseudomonas putida (strain KT2440) GN=nuoG PE=3 SV=1 |
39| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RS2_PSEP1 | 138 | 30S ribosomal protein S2 OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rpsB PE=3 SV=1 |
40| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | SAHH_PSEPF | 131 | Adenosylhomocysteinase OS=Pseudomonas fluorescens (strain Pf0-1) GN=ahcY PE=3 SV=1 |
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